期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 166, 期 15, 页码 1700-1704出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2009.04.003
关键词
Citrus; Monoterpene synthase; Sabinene
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
We previously isolated two putative monoterpene synthase genes, RlemTPS1 and RlemTPS2, from rough lemon (Citrus jambhiri) and showed that gene expression of RlemTPS2 was induced by microbial attack. The protein product of RlemTPS2 was obtained using a prokaryotic expression system, and GC and GC-MS of monoterpene synthesis by RlemTPS2 determined that RlemTPS2 encodes a sabinene synthase. Sabinene has antifungal activity toward Alternaria alternata. Furthermore, site-directed mutagenesis identified one amino acid, lie, located at the front of the metal. ion binding motif as an important residue for the product specificity of sabinene synthase. (C) 2009 Elsevier GmbH. All rights reserved.
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