期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 166, 期 5, 页码 499-506出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2008.07.007
关键词
Glyceraldehyde-3-phosphate dehydrogenase; Hordeum vulgare; Lipids; Phospholipase D; Potassium deprivation
资金
- Tunisian Ministry of Higher Education, Scientific Research and Technology [LR02CB02]
To verify the possible involvement of lipids and several other compounds including hydrogen peroxide (H2O2) and glyceraLdehyde-3-phosphate dehydrogenase (G3PDH) in the response of Hordeum vulgare to early potassium deprivation, plants were grown in hydroponic conditions for 30d with a modified Hewitt nutrient solution containing 3 mM K+. They were then incubated for increasing periods of time ranging from 2 to 36h in the same medium deprived of K+. In contrast to leaves, root K+ concentration showed its greatest decrease after 6 h of treatment. The main lipids of the control barley roots were phospholipids (PL), representing more than 50% of the total lipids. PL did not change with treatment, whereas free sterols (FS) decreased following K+ deprivation, showing a reduction of approximately 17% after 36 h. With respect to the individual PL, 30 h K+ deprivation led to a reduction in phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylinositol (PI) levels, whereas phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and phosphatidic acid (PA) levels increased. The maximum PA accumulation and the highest phospholipase D (PLD) activation, estimated by an accumulation of phosphatidylbutanol (PtBut), were observed after 24 h of K+ starvation. At the root level, H2O2 showed the maximum value after 6h of incubation in -K solution. In parallel, G3PDH activity reached its minimum. On the basis of a concomitant stimulation of PLD activity and, consequently, PA accumulation, enhancement of H2O2 production, and inhibition of G3PDH activity, we suggest a possible involvement of these three compounds in an early response to K+ deprivation. (C) 2008 Elsevier GmbH. All rights reserved.
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