期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 166, 期 8, 页码 887-891出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2008.11.001
关键词
L-Phenylalanine; Phenylacetaldehyde; 2-Phenylethanol; Protoplast; [2, 3, 4, 5, 6-C-13(5)]shikimic acid
资金
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan
The isolated protoplasts of rose flowers were used to investigate the metabolic pathway in rose flower leading from shikimic acid or L-phenylatanine (L-Phe) to 2-phenylethanol (2PE), a dominant volatile compound in hybrid roses such as Rosa damascena Mill., R. 'Hoh-Jun', and R. 'Yves Piaget'. Deuterium-labeled L-Phe ([H-2(8)]L-Phe) was supplied to the protoplasts isolated from R. 'Yves Piaget' petals. The volatile end products ([H-2(n)]-2PE, n = 6-8) and their related intermediates ([H-2(n)]phenylacetaldehyde, n = 6-8) were detected in the protoplasts by gas chromatography-mass spectrometry (GC-MS). In addition, we chemically synthesized [2,3,4,5,6-C-13(5)]shikimic acid, a new stable isotopomer, to investigate the formation of 2PE from shikimic acid by GC-MS and nuclear magnetic resonance. We proposed the hypothetical biochemical pathway of 2PE from shikimic acid via chorismic acid, L-Phe, and phenylacetaldehyde. This protoplast system facilitates findings of metabolic intermediates and simplifies the complex branching biosynthetic pathways of floral scents to distinct individual events. (C) 2008 Elsevier GmbH. All rights reserved.
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