Article
Chemistry, Physical
Mariska Thoresen, Samkelo Malgas, Mpho Stephen Mafa, Brett Ivan Pletschke
Summary: This study evaluated and compared the synergistic relationships between cellulases from different microbial sources, finding that not all endo-to-exo-cellulase interactions are synergistic. Maximizing compatibility between exo- and endo-cellulases is critical for designing improved synergistic cellulose-degrading cocktails for industrial-scale biomass degradation.
Article
Plant Sciences
Lavanya Mendu, Gayani Jalathge, Kamalpreet Kaur Dhillon, Nagendra Pratap Singh, Vimal Kumar Balasubramanian, Rebecca Fewou, Dennis C. Gitz, Junping Chen, Zhanguo Xin, Venugopal Mendu
Summary: The present study identified and characterized the function of the endo-1,4-beta-glucanase gene in sorghum, laying the foundation for future research on cell wall biosynthesis and biofuel production.
Article
Biochemistry & Molecular Biology
Huabiao Miao, Yuanhao Zhao, Yu Ma, Nanyu Han, Yuanyuan Zhe, Xianghua Tang, Zunxi Huang
Summary: In this study, the thermostability of CMCase-I enzyme was improved by engineering and inserting the C2 submodule. The resulting C2-CMCase-I chimera showed significantly enhanced stability and the formation of a new beta-sheet structure. This chimera has potential for industrial applications.
PROCESS BIOCHEMISTRY
(2022)
Article
Plant Sciences
Yang Shao, Ping An, Xiaohui Feng, Irshad Muhammad, Victoria Otie, Weiqiang Li, Yuanrun Zheng, Yunus Qiman
Summary: In salt-sensitive wheat cultivars, the elasticity of the root cell wall significantly decreased under salinity stress, while the elasticity was maintained in salt-tolerant cultivars. Root extension was largely dependent on elastic extension, and changes in chemical composition of the cell wall corresponded with cell wall extensibility and root growth in wheat cultivars with different degrees of salt tolerance.
PLANT GROWTH REGULATION
(2021)
Article
Biochemistry & Molecular Biology
Shumao Chai, Xueliang Zhang, Yuejiao Gao, Lishuang Nie, Chenchen Xiang, Mingming Cao, Shaochen Wang, Zhiyang Feng
Summary: A novel endo-/β-1,4-glucanase ZFYN1413-87 with strong enzyme activity was identified and studied in this research, showing 52% retained activity even after treatment in pH 3.0 buffer for 2 hours, representing a unique example in the GH6 family.
CARBOHYDRATE RESEARCH
(2021)
Article
Biochemical Research Methods
Takaaki Fujiwara, Ayumi Fujishima, Yui Nakamura, Kenji Tajima, Min Yao
Summary: This study investigates the hydrolytic activity and crystal structures of an endo-beta-1,4-glucanase from a bacteria that produces bacterial cellulose. The findings provide insights into the reaction process of the enzyme and propose a beta-1,4-glucan-trimming mechanism.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Food Science & Technology
Marinela Sokarda Slavic, Milan Kojic, Aleksandra Margetic, Marina Ristovic, Marija Pavlovic, Stefan Nikolic, Zoran Vujcic
Summary: The study focused on the impact of the enzyme endo-beta-1,3-1,4-glucanase from Bacillus subtilis 168 on improving the nutritional and bioactive properties of barley beta-glucan. A new procedure for isolating pure beta-glucan from barley flour was developed, enabling it to be free from starch contamination. The cloned enzyme showed high efficiency in degrading beta-glucan and increasing its antioxidant capacities, suggesting potential applications in functional foods.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hebin Li, Qingsong Hu, Xuan Hong, Zedong Jiang, Hui Ni, Qingbiao Li, Yanbing Zhu
Summary: MaCel5A is an endo-beta-1,4-glucanase isolated from Microbulbifer sp. ALW1, showing selective activity towards CMC and barley beta-glucan. It exhibits remarkable tolerance to high temperature and salt concentrations, making it a promising candidate for industrial applications.
Article
Microbiology
Do Young Kim, Jonghoon Kim, Yung Mi Lee, Soo Min Byeon, Jeong Hae Gwak, Jong Suk Lee, Dong-Ha Shin, Ho-Yong Park
Summary: The gene coding for an extracellular endo-beta-1,4-glucanase (GluL) from Antarctic lichen-associated bacterium was identified and expressed in Escherichia coli BL21. The recombinant enzyme showed high specificity and cold-adapted activity, making it a promising candidate for low-temperature processes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Vasileios Bampidis, Giovanna Azimonti, Maria de Lourdes Bastos, Henrik Christensen, Birgit Dusemund, Mojca Fasmon Durjava, Maryline Kouba, Marta Lopez-Alonso, Secundino Lopez Puente, Francesca Marcon, Baltasar Mayo, Alena Pechova, Mariana Petkova, Fernando Ramos, Yolanda Sanz, Roberto Edoardo Villa, Ruud Woutersen, Noel Dierick, Giovanna Martelli, Jaume Galobart, Jordi Ortuno, Montserrat Anguita
Summary: As requested by the European Commission, EFSA evaluated the safety and efficacy of the feed additive ROVABIO(R) ADVANCE containing endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase. The additive was found to be safe for weaned piglets and pigs for fattening, posing no concern for consumer safety. It is not irritant to the skin or eyes but can cause sensitization. No environmental risks were identified. However, due to insufficient data, the efficacy of the additive could not be concluded.
Article
Food Science & Technology
Efsa Panel On Food Contact Materials, Claude Lambre, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Yrjo Roos, Natalia Kovalkovicova, Yi Liu, Simone Lunardi, Giulio Di Piazza, Rita Ferreira de Sousa, Andrew Chesson
Summary: This article introduces a food enzyme produced by a non-genetically modified Trichoderma reesei strain AR-256 and used in seven food manufacturing processes. The estimated dietary exposure of the enzyme indicates that it does not raise safety concerns under the intended conditions of use. However, individuals with salmon allergy may have a risk of allergic reactions.
Article
Microbiology
Hadjer Djelid, Sigrid Flahaut, Corinne Vander Wauven, Yamina Oudjama, Serge Hiligsmann, Bertrand Cornu, Radia Cherfia, Maroua Gares, Noreddine Kacem Chaouche
Summary: This study investigated the production of an endo-1,4-beta-glucanase by a potential carboxymethylcellulose-hydrolyzing bacterial strain H1 using untreated olive mill wastes as substrate. The enzyme was purified and characterized, showing high enzymatic activity, thermostability, and halotolerance. It also demonstrated the ability to release cello-oligosaccharides. These findings suggest that the H1 endo-1,4-beta-glucanase may have significant commercial values for industry, argo-waste treatment, and other biotechnological applications.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Antri Georgiou, Simon Sieber, Chien-Chi Hsiao, Tatyana Grayfer, Jacob L. Gorenflos Lopez, Karl Gademann, Leo Eberl, Aurelien Bailly
Summary: After a century of research, it was found that the obligate beta-proteobacterial endosymbionts in leaf nodules of tropical Rubiaceae supply the alpha-D-glucose analogue (+)-streptol to their Psychotria hosts, inhibiting root growth selectively. The compound appears to specifically target meristematic root cells by affecting cell wall organization, potentially serving as an allelochemical. Moreover, the suppression of plant competitors' growth is proposed as a major factor in the formation and maintenance of the Psychotria-Burkholderia association.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Vasileios Bampidis, Giovanna Azimonti, Maria de Lourdes Bastos, Henrik Christensen, Birgit Dusemund, Mojca Fasmon Durjava, Maryline Kouba, Marta Lopez-Alonso, Secundino Lopez Puente, Francesca Marcon, Baltasar Mayo, Alena Pechova, Mariana Petkova, Fernando Ramos, Yolanda Sanz, Roberto Edoardo Villa, Ruud Woutersen, Jaume Galobart, Elisa Pettenati, Fabiola Pizzo, Joana Revez, Montserrat Anguita
Summary: Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) provided a scientific opinion on the renewal of the authorization of two enzyme additives produced by Trichoderma reesei for poultry and pigs. The Panel concluded that the additives comply with the current authorization conditions and have the potential to be efficacious, but may cause eye irritation and respiratory sensitization. However, no conclusions could be drawn on the skin irritancy/sensitization potential of the additives.
Article
Biology
Mohammad Mosleh Uddin, Suzana Afrin Lima, Tanim Jabid Hossain, Newton Kar, Yeasmin Zahan, Babamale AbdulKareem Olarewaju
Summary: Insects of the order Coleoptera are known for their cellulolytic activity, with this study revealing the presence of cellulolytic endo-1,4-beta-D-glucanase activity in the gut fluid of Hoplasoma unicolor. The qualitative and quantitative analysis showed optimal activity at pH 5.3, suggesting the potential industrial applications of the cellulase enzymes found in this insect.
TROPICAL LIFE SCIENCES RESEARCH
(2021)
Article
Plant Sciences
Ko Hirano, Reiko Masuda, Wakana Takase, Yoichi Morinaka, Mayuko Kawamura, Yoshinobu Takeuchi, Hiroki Takagi, Hiroki Yaegashi, Satoshi Natsume, Ryohei Terauchi, Toshihisa Kotake, Yasuyuki Matsushita, Takashi Sazuka
Article
Agronomy
Kotaro Miura, Eiji Yamamoto, Yoichi Morinaka, Tomonori Takashi, Hidemi Kitano, Makoto Matsuoka, Motoyuki Ashikari
Article
Plant Sciences
Ko Hirano, Koichiro Aya, Yoichi Morinaka, Shiro Nagamatsu, Yutaka Sato, Baltazar A. Antonio, Nobukazu Namiki, Yoshiaki Nagamura, Makoto Matsuoka
PLANT AND CELL PHYSIOLOGY
(2013)
Article
Plant Sciences
Ko Hirano, Koichiro Aya, Mari Kondo, Ayako Okuno, Yoichi Morinaka, Makoto Matsuoka
PLANT CELL REPORTS
(2012)
Article
Plant Sciences
Tomoaki Sakamoto, Yoichi Morinaka, Yoshiaki Inukai, Hidemi Kitano, Shozo Fujioka
Article
Multidisciplinary Sciences
Ayako Okuno, Ko Hirano, Kenji Asano, Wakana Takase, Reiko Masuda, Yoichi Morinaka, Miyako Ueguchi-Tanaka, Hidemi Kitano, Makoto Matsuoka
Article
Multidisciplinary Sciences
Kenji Yano, Yoichi Morinaka, Fanmiao Wang, Peng Huang, Sayaka Takehara, Takaaki Hirai, Aya Ito, Eriko Koketsu, Mayuko Kawamura, Kunihiko Kotake, Shinya Yoshida, Masaki Endo, Gen Tamiya, Hidemi Kitano, Miyako Ueguchi-Tanaka, Ko Hirano, Makoto Matsuoka
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Plant Sciences
Genki Chaya, Shuhei Segami, Moeka Fujita, Yoichi Morinaka, Yukimoto Iwasaki, Kotaro Miura
Summary: This study reveals that both DEP1 and OsGGC2 genes are positive regulators of plant height and their functions are redundant.
Article
Biochemistry & Molecular Biology
Tory Chhun, Koichiro Aya, Kenji Asano, Eiji Yamamoto, Yoichi Morinaka, Masao Watanabe, Hidemi Kitano, Motoyuki Ashikari, Makoto Matsuoka, Miyako Ueguchi-Tanaka
Article
Agronomy
Eiji Yamamoto, Tomonori Takashi, Yoichi Morinaka, Shaoyang Lin, Hidemi Kitano, Makoto Matsuoka, Motoyuki Ashikari
THEORETICAL AND APPLIED GENETICS
(2007)
Article
Plant Sciences
Yuko Yamamoto, Noriko Kamiya, Yoichi Morinaka, Makoto Matsuoka, Takashi Sazuka
Article
Plant Sciences
Yoichi Morinaka, Tomoaki Sakamoto, Yoshiaki Inukai, Masakazu Agetsuma, Hidemi Kitano, Motoyuki Ashikari, Makoto Matsuoka
Article
Plant Sciences
M Hajduch, H Tanaka, Y Morinaka, Y Otake, H Nakamura, T Kayano, Y Koga-Ban
BIOLOGIA PLANTARUM
(2005)
Article
Biotechnology & Applied Microbiology
T Sakamoto, Y Morinaka, K Ishiyama, M Kobayashi, H Itoh, T Kayano, S Iwahori, M Matsuoka, H Tanaka
NATURE BIOTECHNOLOGY
(2003)