期刊
JOURNAL OF PLANT BIOLOGY
卷 54, 期 1, 页码 23-32出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s12374-010-9139-2
关键词
AtGA2ox8; Dwarf; Seed germination; Chlorophyll content; The photosynthesis capacity
资金
- National 863 Plan of China [2007AA10Z127]
- National Natural Science Foundation of China [30800080]
Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing the amount of bioactive GA and hence reduced the plant height. As anticipated, the transgenic plant exhibited dwarf phenotype. Importantly, compared with the wild type, the transgenic plants had delayed the seed germination, increased the chlorophyll content (28.7-36.3%) and photosynthesis capacity (14.3-18.7%) in a single leaf. At the same time, the photosynthesis capacity of the whole plants was significantly enhanced (35.7-48.6%) due to the extra leaves and branches.
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