Article
Biochemistry & Molecular Biology
Hira Maryam, Zulfiqar Ali, Muhammad Abu Bakar Saddique, Fahim Nawaz
Summary: This study identified CYP706B1, CDNC, and 2ODD-1 as the most promising genes involved in gossypol biosynthesis in cotton plants. It also found that developing boll shell, developing embryo, leaf, and sepal have significant ability to synthesize gossypol. These findings provide a way for scientists to manipulate gossypol contents in economically important organs of cotton plant through targeted breeding.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yue Sun, Yifei Han, Kuang Sheng, Ping Yang, Yuefen Cao, Huazu Li, Qian-Hao Zhu, Jinhong Chen, Shuijin Zhu, Tianlun Zhao
Summary: The comprehensive utilization of cottonseeds is limited by the presence of pigment glands and gossypol. This study investigated the development of pigment glands in G. bickii, a wild cotton species, and identified the regulatory mechanisms involved. Single-cell RNA sequencing revealed the presence of distinct cell populations, including pigment gland cells, secretory cells, and apoptotic cells. Light and gibberellin were found to promote pigment gland formation, and three novel genes were identified to affect this process. These findings provide new insights into pigment gland morphogenesis and lay the foundation for future scRNA-seq investigations in cotton.
Article
Biochemistry & Molecular Biology
Yuanli Dai, Shang Liu, Dongyun Zuo, Qiaolian Wang, Limin Lv, Youping Zhang, Hailiang Cheng, John Z. Yu, Guoli Song
Summary: This study conducted a systematic analysis of MYB genes in cotton pigment gland development and identified certain members that may play a role in cotton pigment gland formation and gossypol biosynthesis. Through weighted gene co-expression network analysis, MYB genes related to gland development and gossypol biosynthesis were identified. These findings provide candidate genes for further research on the roles of MYB genes in cotton pigment gland formation, gossypol biosynthesis, and future crop improvement.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Plant Sciences
Yihao Zang, Chenyu Xu, Lisha Xuan, Lingyun Ding, JianKun Zhu, Zhanfeng Si, Tianzhen Zhang, Yan Hu
Summary: The study identified a novel stem pigment gland-forming gene, GoSPGF, responsible for glandless trait on the stem in cotton. Loss of GoSPGF significantly affects gossypol biosynthesis and alters the expression of related genes. This discovery provides insights into the regulation of glandular structure differentiation and formation in cotton, with potential implications for other plants with special gland structures.
Article
Agricultural Engineering
Lijiao Gu, Fei Wei, Pengyun Chen, Minsheng Yang, Zhi Liu
Summary: This study conducted a comprehensive analysis of KIN genes in cotton, identifying 172 putative GhKIN genes and classifying them into eight subfamilies. These GhKIN genes showed uneven distribution on chromosomes and their expansion was driven by segmental duplication. Different subfamily genes exhibited differences in gene structure and motif composition, but similarities were observed within the same subfamily. The promoter regions of GhKIN genes contained stress- and hormone-related cis-elements.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Biochemistry & Molecular Biology
Masood Jan, Zhixin Liu, Chenxi Guo, Yaping Zhou, Xuwu Sun
Summary: Cotton is a plant that can produce spinnable fibers. There are 50 described species of cotton, with only four species being cultivated and the rest being wild. The black structures on the surface of cotton organs, called gossypol glands or pigment glands, store terpenoid aldehydes. These glands provide a system for studying cell differentiation and organogenesis, but the genes and molecular mechanisms involved in gland formation are still not fully understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Ruifeng Cui, Xiaoge Wang, Waqar Afzal Malik, Xuke Lu, Xiugui Chen, Delong Wang, Junjuan Wang, Shuai Wang, Chao Chen, Lixue Guo, Quanjia Chen, Wuwei Ye
Summary: This study identified 74 RAFS genes from G. hirsutum, G. barbadense, G. arboreum and G. raimondii, and found that the gene family could be divided into four major clades. The gene structure was relatively conservative, and some genes might be regulated by gibberellins and abscisic acid. Transcriptome analysis suggested that genes in clade III are highly expressed in organs such as seed, root, cotyledon, ovule and fiber, and are involved in resistance to abiotic stress. Gene co-expression network analysis showed that key genes like GhRFS2A-GhRFS6A, GhRFS6D, GhRFS7D, and GhRFS8A-GhRFS11A have high expression levels under salt, drought, cold and heat stress.
BMC BIOINFORMATICS
(2021)
Article
Plant Sciences
Zhengxiu Ye, Lu Qiao, Xiangyin Luo, Xinyuan Chen, Xianlong Zhang, Lili Tu
Summary: The study identified 164 genes encoding GRAM domain proteins in four cotton species. Experimental results showed that GhGRAM31 may control fiber length, interact with other GRAM domain proteins, and participate in the regulation of multiple transcription factors.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Kai Cheng, Cangbao Lei, Siyuan Zhang, Qiao Zheng, Chunyan Wei, Weiyi Huang, Minghui Xing, Junli Zhang, Xiangyu Zhang, Xiao Zhang
Summary: In this study, the authors identified and analyzed the PRC2 core components in cotton, particularly upland cotton. They investigated their classifications, phylogenetic relationships, gene structures, domain organizations, subcellular localizations, protein interactions, tissue-specific expression patterns, and stress responsiveness. These findings provide insights into the evolution and composition of cotton PRC2 and lay the foundation for further research on their biological functions and regulatory mechanisms.
Article
Plant Sciences
Devendra Pandeya, LeAnne M. Campbell, Lorraine Puckhaber, Charles Suh, Keerti S. Rathore
Summary: Using a graft combination of Ultra-low gossypol cottonseed as a scion, it was confirmed that roots are not a source of terpenoids in the aboveground parts of a cotton plant. Gossypol and related terpenoids are present in the lysigenous glands in the aboveground parts of a cotton plant. However, roots produce significant amount of gossypol and different terpenoids. This study showed that the roots cannot replenish the gossypol content of the cottonseed-glands.
Article
Biochemistry & Molecular Biology
Muhammad Naeem, Muhammad Iqbal, Sami Ul-Allah, Hassan Javed Chaudhary, Wajad Nazeer, Javeria Ashraf, Faheem Shahzad Baloch
Summary: The study aimed to identify cotton genotypes with prominently expressed stress responsive genes. Evaluation of four cotton genotypes under drought and salinity stress conditions revealed pronounced expression of stress responsive genes in variety IUB-13, followed by IUB-222, suggesting their potential to cope with environmental hazards. Utilization of these genotypes in cotton breeding programs can be fruitful for developing varieties adaptable to climate change.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Plant Sciences
Maria E. Lisei-de-Sa, Paolo L. Rodrigues-Silva, Carolina Morgante, Bruno Paes de Melo, Isabela T. Lourenco-Tessutti, Fabricio B. M. Arraes, Joao P. A. Sousa, Rafael Galbieri, Regina M. S. Amorim, Camila B. J. de Lins, Leonardo L. P. Macedo, Valdeir J. Moreira, Gilanna F. Ferreira, Thuanne P. Ribeiro, Rodrigo R. Fragoso, Maria C. M. Silva, Janice de Almeida-Engler, Maria F. Grossi-de-Sa
Summary: Host-induced gene silencing (HIGS) targeting essential genes of root-knot nematode can significantly reduce gall formation and egg masses production, as well as improve cotton plant tolerance to Meloidogyne incognita infection without phenotypic penalty in transgenic plants.
Article
Agronomy
Dexin Liu, Xueying Liu, Yao Su, Xiao Zhang, Kai Guo, Zhonghua Teng, Jian Zhang, Dajun Liu, Zhengsheng Zhang
Summary: The study identified the gene GhMYB9 in Gossypium hirsutum plant as a key contributor to green pigments in cotton fibers. Silencing GhMYB9 resulted in white or light green fuzz in cotton fibers.
Article
Plant Sciences
Li Liu, Dan Wang, Jinping Hua, Xianhui Kong, Xuwen Wang, Juan Wang, Aijun Si, Fuxiang Zhao, Wenhao Liu, Yu Yu, Zhiwen Chen
Summary: In this study, three carbon-chain extension genes associated with fatty acid synthesis in upland cotton were cloned and characterized. These genes play important roles in oil accumulation in cotton seeds and fiber elongation. The expression patterns of these genes varied during different stages of seed development, and their overexpression resulted in increased oil content.
Article
Biochemistry & Molecular Biology
Zhen Peng, Xuran Jiang, Zhenzhen Wang, Xiaoyang Wang, Hongge Li, Shoupu He, Zhaoe Pan, Abdul Qayyum, Abdul Rehman, Xiongming Du
Summary: This study identified RAF genes in different cotton species and suggested that cotton evolution experienced purifying selection of the RAF gene family. The analysis of synteny and gene collinearity indicated that dispersed and segmental duplication events contributed to the expansion of RAFs in cotton.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
