Article
Agronomy
Chamran Hemmati, Mehrnoosh Nikooei, Abdullah M. Al-Sadi
Summary: The study found that infection of phytoplasmas in Hishimonus phycitis leads to higher fecundity and nymph developmental rate, contributing to an increased overall fitness of the infected population. Additionally, infected female insects had lower survival rates compared to infected males, suggesting a potential fitness trade-off in response to phytoplasma infection.
Article
Plant Sciences
A. M. Al-Subhi, A. M. Al-Sadi, R. A. Al-Yahyai, Y. Chen, T. Mathers, Z. Orlovskis, G. Moro, S. Mugford, K. S. Al-Hashmi, S. A. Hogenhout
Summary: The Witches' Broom Disease of Lime (WBDL) caused by an insect-transmitted phytoplasma has led to a significant decrease in lime production in multiple countries. Research has shown that the gene encoding SAP11 of WBDL phytoplasma interacts with lime TCP transcription factors, inducing witches' broom symptoms and attracting insect vectors. High phytoplasma titers and expression levels of SAP11 were found in symptomatic leaves, suggesting that affected parts of trees contribute to WBDL epidemics by supporting higher phytoplasma levels and attracting vectors.
Article
Microbiology
Behcet Kemal Caglar, Serkan Pehlivan, Ekrem Atakan, Toufic Elbeaino
Summary: In two corn fields in Imamoglu town, Turkey, maize plants showed phytoplasma-like symptoms. Through PCR and sequence analysis, it was identified as Ligustrum witches' broom phytoplasma (LiWBP) with a distant relationship to 16SrII and 16SrXXV groups. This is the first report of LiWBP infecting maize and causing phyllody disease in Turkey, providing new molecular insights into its phylogenetic position.
Article
Plant Sciences
Leila Rastegar, Maryam Ghayeb Zamharir, Wen-Jing Cai, Hossein Mighani, Alireza Ghassempour, Yu-Qi Feng
Summary: The study found that in Mexican lime trees infected with lime witches'-broom phytoplasma, using Previcur Energy(TM) can induce plant resistance and reduce the severity of the disease. Additionally, the phytoplasma and resistance inducer may affect fundamental processes in plants through regulation of phytohormone levels.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
Article
Agriculture, Multidisciplinary
Behcet Kemal Caglar, Eray Simsek, Toufic Elbeaino
Summary: A study in Tufanbeyli, Adana, Turkey revealed an emerging witches' broom disease in juniper trees, caused by the Ligustrum witches' broom phytoplasma (LiWBP) and a newly discovered subgroup lineage. PCR analysis showed a high percentage of infected juniper trees and their nucleotide sequences showed a high degree of similarity to LiWBP. This study highlighted the unique evolutionary lineage and genetic divergence of this phytoplasma.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2023)
Article
Microscopy
Junhyung Park, Hyo-Jeong Kim, Yang Hoon Huh, Ki Woo Kim
Summary: The study revealed the presence of multivesicular bodies (MVBs) in phytoplasma-infected jujube leaves, connected to the plasma membrane appressed to the cell wall. Diseased leaves showed increased pore-plasmodesma unit thickness in sieve elements compared to control leaves. Phytoplasma produced pleomorphic cells in sieve elements with various extracellular structures observed, providing valuable insights into intracellular obligate cell wall-less prokaryotes and host phloem structures.
Article
Plant Sciences
Sina Noorizadeh, Reza Khakvar, Morteza Golmohammadi, Seyed Mehdi Bani Hashemian, Mohammad Mehdi Faghihi
Summary: The study revealed that Witches' broom disease of lime in Iran is associated with ribosomal subgroup 16SrII-B, with three genotypes recognized based on 16Sr virtual RFLP profiles. Phylogenetic analysis based on the rp and ftsH gene sequences showed clustering of all the strains as one group. The analysis of group II intron genes demonstrated the highest differentiation among 'Ca. P. aurantifolia'-related strains, indicating a higher genetic diversity.
TROPICAL PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Hamidreza Kabiri, Majid Siampour, Ghobad Babaei
Summary: The host range of two Iranian AlmWB phytoplasma strains, PEAB and AlKI, were examined through graft-inoculation assay. Both strains could infect almond, peach and plum seedlings, with more obvious symptoms in almond cultivars. The strains could not be detected in sweet cherry and Myrobalan plum seedlings. In addition, infection of apricot seedlings could only be detected in those inoculated with the PEAB strain.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Agronomy
Lolita M. Dolores, Joanne A. Langres, Marita S. Pinili, Merdelyn T. Caasi-Lit, Cris Q. Cortaga, Yron M. Retuta, Fe M. Dela Cueva
Summary: Cassava witches' broom (CWB) disease, caused by Candidatus Phytoplasma luffae 16SrVIII, is a major challenge for the cassava industry in the Philippines. This study reveals the widespread presence of CWB in the country and provides important information about the genetic diversity and structure of the phytoplasma strain. The findings contribute to a better understanding of the etiology of CWB and can help in developing effective strategies for disease detection and management.
Article
Immunology
R. Abirami, S. K. Manoranjitham, S. Mohankumar, G. Karthikeyan
Summary: Cucumber mosaic virus (CMV) and Candidatus Phytoplasma australasia (Ca. P. australasia) were found to cause mixed infection in brinjal plants in India. The study also identified Solarium torvum as a host for CMV infection. These findings are significant for the research and disease control in brinjal cultivation.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Nicolas Quiroga, Camila Gamboa, Gabriela Medina, Nicoletta Contaldo, Fernando Torres, Assunta Bertaccini, Alan Zamorano, Nicola Fiore
Summary: The economic losses caused by 'Candidatus Liberibacter' and 'Candidatus Phytoplasma' in citrus have raised concerns in citrus-producing regions worldwide. These bacteria have not been reported in citrus species in Chile. However, during the years 2017 and 2019, 14 plants were found to be positive for previously unidentified phytoplasmas in citrus.
Article
Horticulture
Mingsheng Deng, Fuli Ma, Xinyue Zhang, Jinqiu Huang, Jian Yang, Mengting Chen, Junyong Zhou, Qibao Sun, Jun Sun
Summary: Jujube witches' broom (JWB), a devastating disease caused by 'Candidatus Phytoplasma ziziphi', induces symptoms such as phyllody in infected plants. Effector proteins secreted by phytoplasmas play a key role in inducing these symptoms, with SJP3 identified as a potential effector inducing phyllody in infected jujube plants. The study sheds light on the pathogenesis of JWB disease and its interaction with MADS-box genes related to floral organ identity and flowering time.
SCIENTIA HORTICULTURAE
(2021)
Article
Biology
De-Zhi Kong, Cai-Li Lin, Shao-Shuai Yu, Guo-Zhong Tian, Hai-Bin Ma, Sheng-Jie Wang
Summary: This study investigated the molecular diversity of Paulownia witches'-broom (PaWB) phytoplasmas from different geographical regions using multilocus sequence typing (MLST). The results showed high genetic diversity among the PaWB strains, and revealed geographical differentiation and genetic relatedness. The findings provide valuable information for epidemiological studies and PaWB disease control decisions.
Article
Biochemistry & Molecular Biology
Junqiang Yang, Zhongmei Shen, Pengyan Qu, Rui Yang, Anping Shao, Hao Li, Ailing Zhao, Chunzhen Cheng
Summary: This study reported the therapeutic effect of oxytetracycline hydrochloride (OTC-HCl) on jujube witches' broom disease (JWB) and revealed the impact of JWB infection and treatment on gene expression profiling in jujube.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Junyong Zhou, Fuli Ma, Yu Yao, Mingsheng Deng, Mengting Chen, Shanqi Zhang, Yamei Li, Jian Yang, Ning Zhang, Jingqiu Huang, Qibao Sun, Jun Sun
Summary: The study showed that two phytoplasma effectors, SJP1 and SJP2, induce witches' broom and increase lateral branches in jujube trees by repressing the auxin efflux channel. This finding suggests a potential strategy for controlling phytoplasma-associated diseases and a resource for creating new cultivars with varying degrees of shoot branching through gene editing breeding.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Virology
Kajal Kumar Biswas, Supratik Palchoudhury, Prosenjit Chakraborty, Utpal K. Bhattacharyya, Dilip K. Ghosh, Palash Debnath, Chandrika Ramadugu, Manjunath L. Keremane, Ravi K. Khetarpal, Richard F. Lee
Article
Biochemistry & Molecular Biology
Pranav Kumar, Vikram Dalal, Amol Kokane, Sweta Singh, Sapna Lonare, Harry Kaur, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biotechnology & Applied Microbiology
Sunil B. Kokane, Sumit Bhose, Amol Kokane, Mrugendra Gubyad, Dilip Kumar Ghosh
Article
Plant Sciences
Dilip Kumar Ghosh, Amol D. Kokane, Sunil B. Kokane, Jigme Tenzin, Mrugendra G. Gubyad, Phuntsho Wangdi, Ashutosh A. Murkute, Ashwani Kumar Sharma, Siddarame Gowda
Summary: Citrus, especially mandarin, is an economically important fruit crop in Bhutan, but faces challenges of early decline and low productivity due to the presence of Candidatus Liberibacter asiaticus and citrus tristeza virus (CTV). Molecular analysis revealed a high incidence of these pathogens in symptomatic citrus trees, providing valuable insights into their association with citrus decline in Bhutan.
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Biochemical Research Methods
Sunil B. Kokane, Amol D. Kokane, Pragati Misra, Ashish J. Warghane, Pranav Kumar, Mrugendra G. Gubyad, Ashwani Kumar Sharma, Kajal Kumar Biswas, Dilip Kumar Ghosh
MOLECULAR AND CELLULAR PROBES
(2020)
Article
Multidisciplinary Sciences
Dilip Kumar Ghosh, Sunil B. Kokane, Siddarame Gowda
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Vikram Dalal, Brajesh Kumar Savita, Poonam Dhankhar, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma
Summary: The study utilized structure-based drug design approach to screen and identify potent molecules against 2Cys Prx, which showed higher binding affinity with Prx compared to its substrate. Molecular dynamics analysis revealed that the Prx-inhibitor complexes were more stable.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biotechnology & Applied Microbiology
Sunil B. Kokane, Pragati Misra, Amol D. Kokane, Mrugendra G. Gubyad, Ashish J. Warghane, Datta Surwase, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: Tristeza is an economically important disease of citrus caused by Citrus tristeza virus, which has caused significant losses to the industry worldwide. Studies on virus distribution and tropism in citrus plants are necessary to understand the reason behind this scenario.
Article
Biotechnology & Applied Microbiology
Amol D. Kokane, Kapil Lawrence, Sunil B. Kokane, Mrugendra G. Gubyad, Pragati Misra, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: A RT-qPCR assay targeting a specific region of the ICRSV coat protein gene was developed and optimized for the detection of the virus, showing high specificity and sensitivity. This assay can be useful for certification programs and plant quarantine stations to manage the disease effectively.
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Ruchi Rani, Amol D. Kokane, Dilip Kumar Ghosh, Shailly Tomar, Ashwani Kumar Sharma
Summary: In this study, the recombinant cytoplasmic 2-Cys peroxiredoxin from Citrus sinensis (CsPrx) was purified and characterized. The reduced state of the protein had higher alpha-helical content. CsPrx was found to provide protection against ROS and free radicals, and it also promoted cell proliferation and wound healing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Dilip Kumar Ghosh, Amol Kokane, Sunil Kokane, Krishanu Mukherjee, Jigme Tenzin, Datta Surwase, Dhanshree Deshmukh, Mrugendra Gubyad, Kajal Kumar Biswas
Summary: This study detected a high incidence of citrus tristeza virus (CTV) infection in Mandarin orange samples from Bhutan using reverse transcription PCR (RT-PCR). Genetic variability among Bhutanese CTV variants was analyzed by sequencing four independent genomic regions and comparing with GenBank deposited sequences. The study identified unexpected diversity among the Bhutanese CTV variants and also discovered a resistant-breaking variant. The findings contribute to the understanding of CTV diversity and provide insights into pathogenicity assessments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Vikram Dalal, Brajesh Kumar Savita, Md Shahid Alam, Anamika Singh, Mrugendra Gubyad, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma
Summary: In this study, the Bacterioferritin comigratory protein family 1 Cys peroxiredoxin from Candidatus Liberibacter asiaticus (CLaBCP) was biochemically studied and screened for potential antibacterial molecules. The results showed that CLaBCP has enzymatic activity and undergoes structural changes at different pH and temperature ranges. A structure-based drug design method was used to identify more efficient molecules, and molecular dynamics studies confirmed their stability.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Plant Sciences
Dilip Ghosh, Sunil Kokane, Brajesh Kumar Savita, Pranav Kumar, Ashwani Kumar Sharma, Ali Ozcan, Amol Kokane, Swadeshmukul Santra
Summary: Huanglongbing (HLB), also known as citrus greening, is a devastating disease of citrus trees caused by Candidatus Liberibacter asiaticus (CLas) bacteria, which are transmitted by psyllid vectors. Current control measures for HLB are ineffective in the long term, leading to difficulty in preventing disease spread. To combat HLB, a multipronged management strategy is necessary, targeting the pathogen, host, and vector.
Article
Biochemistry & Molecular Biology
Pranav Kumar, Vikram Dalal, Nidhi Sharma, Sunil Kokane, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma