期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 596, 期 22, 页码 5307-5318出版社
WILEY
DOI: 10.1113/JP276615
关键词
medial habenula; uncaging; acetylcholine
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM053395] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS069720] Funding Source: NIH RePORTER
- Deutsche Forschungsgemeinschaft (DFG) [N/A] Funding Source: Medline
- NIGMS NIH HHS [R01 GM053395] Funding Source: Medline
- NIH Funding Source: Medline
- NINDS NIH HHS [R01 NS069720] Funding Source: Medline
Photochemical release of neurotransmitters has been instrumental in the study of their underlying receptors, with acetylcholine being the exception due to its inaccessibility to photochemical protection. We caged a nicotinic acetylcholine receptor (nAChR) agonist, ABT594, via its secondary amine functionality. Effective photolysis could be carried out using either one- or two-photon excitation. Brief flashes (0.5-3.0 ms) of 410 nm light evoked large currents and Ca2+ transients on cell bodies and dendrites of medial habenula (MHb) neurons. Unexpectedly, photorelease of ABT594 also revealed nAChR-mediated Ca2+ signals along the axons of MHb neurons.
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