Article
Cell Biology
Rutambhara Purohit, Amal Kanti Bera
Summary: The study investigated the effect of Panx1 on P2X7R-mediated Ca2+ influx, and found that Panx1 attenuated the rise in Ca2+ concentration mediated by P2X7R, while also preventing cell death caused by P2X7R overactivation.
Article
Multidisciplinary Sciences
Juliana C. Correa-Velloso, Paula J. Bartlett, Robert Brumer, Lawrence D. Gaspers, Henning Ulrich, Andrew P. Thomas
Summary: Rat hepatocytes express multiple purinergic P2Y and P2X receptors, leading to distinct Ca2+ oscillations. These oscillation patterns are shaped by protein kinase C, resulting in unique cellular responses to different agonists.
Review
Biochemistry & Molecular Biology
James B. Ames
Summary: CaM and CaBP1 interact with CaV through binding to the IQ motif to oppositely control Ca2+-dependent channel activity, with CaM promoting CDI and CaBP1 preventing CDI and promoting CDF.
Article
Biochemistry & Molecular Biology
Woori Ko, Byung-Chang Suh
Summary: The membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) selectively modulates the gating of the TMEM16A(ac) channel in an agonistic manner, leading to the upregulation of TMEM16A(ac) functions in physiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Lu Wang, Sebastien Roger, Xuebin B. Yang, Lin-Hua Jiang
Summary: MSC release ATP and express various P2X and P2Y receptors, participating in Ca2+ signaling and regulating cell functions. The SOC channel plays a significant role in ATP-induced Ca2+ signaling in MSC.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2021)
Article
Neurosciences
David Castellano, Kunwei Wu, Angelo Keramidas, Wei Lu
Summary: This study demonstrates that Shisa7 regulates the single-channel properties of GABAARs, reducing the frequency of openings and accelerating their deactivation by governing the time spent between close and open states. These findings shed light on the temporal dynamics of GABAergic transmission.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Immunology
Avishay Dolitzky, Inbal Hazut, Shmulik Avlas, Sharon Grisaru-Tal, Michal Itan, Ilan Zaffran, Francesca Levi-Schaffer, Motti Gerlic, Ariel Munitz
Summary: This study demonstrates that eosinophils can interact with apoptotic cells, leading to polarized activation towards an anti-inflammatory phenotype. Apoptotic cells suppress the inflammatory responses of eosinophils and enhance the activation induced by specific cytokines. These findings reveal the heterogeneity of eosinophil activation patterns and highlight the potential role of apoptotic cells in regulating eosinophil polarization.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
A. Catalina Velez-Ortega, Ruben Stepanyan, Stephanie E. Edelmann, Sara Torres-Gallego, Channy Park, Desislava A. Marinkova, Joshua S. Nowacki, Ghanshyam P. Sinha, Gregory I. Frolenkov
Summary: TRPA1 channels are expressed in nociceptive neurons and in the cochlea, and their function in the cochlea is unknown. This study shows that TRPA1 activation in the supporting cells of the cochlea causes prolonged calcium responses, leading to contractions of pillar and Deiters' cells. TRPA1 channels are activated by oxidative stress and extracellular ATP, which are present after acoustic trauma. TRPA1 deficiency results in larger but less prolonged temporary shift in hearing thresholds, indicating its contribution to cochlear sensitivity regulation after noise exposure.
NATURE COMMUNICATIONS
(2023)
Review
Cell Biology
Gerald Thiel, Tobias Schmidt, Oliver G. Roessler
Summary: Ca2+ ions act as second messengers in the regulation of various cellular events, with a non-uniform distribution within the cytoplasm. Elevated Ca2+ concentration changes the genetic expression pattern of the cell by activating different transcription factors. The mode of Ca2+ entry and the role of enzymes as signal transducers play important roles in gene transcription regulation.
Article
Physiology
Francisco Jaque-Fernandez, Gonzalo Jorquera, Jennifer Troc-Gajardo, France Pietri-Rouxel, Christel Gentil, Sonja Buvinic, Bruno Allard, Enrique Jaimovich, Vincent Jacquemond, Mariana Casas
Summary: The study demonstrates functional interactions between muscle contraction and transcriptional activity in skeletal muscle, revealing a bidirectional regulation between Ca(V)1.1 and Panx1. By modulating the relationship between these proteins, muscle fiber contraction and transcription are affected.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Erika K. Harding, Ivana A. Souza, Maria A. Gandini, Vinicius M. Gadotti, Md Yousof Ali, Sun Huang, Flavia T. T. Antunes, Tuan Trang, Gerald W. Zamponi
Summary: Cannabinoids are a potential therapy for chronic pain, but their analgesic efficacy is often not reported in clinical trials. This study found that cannabinoids can produce analgesia by inhibiting voltage gate calcium channels, and different cannabinoids target different types of channels through distinct mechanisms. The cannabinoid CBD binds to and occludes the pore of the Ca(v)3.2 channel, alleviating inflammatory and neuropathic pain.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biology
Michael W. Country, Michael G. Jonz
Summary: By studying the responses of goldfish and rainbow trout retinal cells to hypoxia, it was found that goldfish maintain [Ca2+](i) stably by using mitochondrial ATP-dependent K+ channels (mK(ATP)) to resist excitotoxicity, while rainbow trout experience a rapid increase in [Ca2+](i) during hypoxia, which can be life-threatening.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Guizhen Fan, Mariah R. Baker, Lara E. Terry, Vikas Arige, Muyuan Chen, Alexander B. Seryshev, Matthew L. Baker, Steven J. Ludtke, David Yule, Irina I. Serysheva
Summary: In this study, the cryo-EM structures of IP(3)R1 were determined, providing atomic details of IP3, Ca2+ and ATP binding. The authors identified the molecular motions of key domains and sidechains that regulate ligand binding and gating behavior of IP3R channels.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Mengrong Li, Miaomiao Li, Jingjing Guo
Summary: Extensive molecular dynamics simulations of PTH1R-PTH complexes reveal that Ca2+ ions play a crucial role in regulating PTH1R activation through interactions with specific acidic residues, inducing protein conformational changes, and enhancing residue-residue communication.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)