期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 589, 期 13, 页码 3309-3318出版社
WILEY
DOI: 10.1113/jphysiol.2011.209726
关键词
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资金
- National Institute on Aging [5R03AG033245]
- Arkansas Biosciences Institute
- Arkansas Tobacco Settlements Proceeds Act
The purpose of this study was to characterize neuropeptide Y (NPY) overflow and metabolism from isolated skeletal muscle arterioles of female rats. Gastrocnemius first-order arterioles were removed from young (2 months), young adult (6 months) and middle-aged (12 months) F344 female rats. Arterioles were isolated, cannulated and pressurized in a microvessel bath with field stimulation electrodes. NPY overflow from isolated arterioles was assessed at 0 s and 30 s post-field stimulation. Dipeptidyl peptidase IV (DPPIV) activity was quantified via fluorometric assay of whole vessel homogenate. In young adult and middle-aged rats, NPY overflow increased 0 s and 30 s following field stimulation. In young adult rats, DPPIV inhibition resulted in an increase in NPY overflow at 30 s, while middle-aged rats had no increase in NPY overflow with DPPIV inhibition (P < 0.05). DPPIV activity was influenced by factors such as age, vessel type, and endothelium (P < 0.05). The present data suggest that DPPIV plays a significant role in modulating the actions of NPY in arterioles of young adult females; however, this role appears to diminish with age.
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