Article
Immunology
Chenzhe Gao, Marwa Yagoub Farag Koko, Mingxing Ding, Weichen Hong, Jianping Li, Na Dong, Mizhou Hui
Summary: This study investigated the effects of intestinal alkaline phosphatase (IAP) in controlling intestinal inflammation and alleviating associated insulin resistance (IR). The researchers found that IAP can be used as a natural anti-inflammatory agent to reduce intestinal inflammation-induced IR.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sudha B. Singh, Henry C. Lin
Summary: Intestinal alkaline phosphatase (IAP) is a multifunctional protein that plays a protective role in the gut, with defects leading to various gastrointestinal disorders. Studies using exogenous IAP supplementation and cellular overexpression have helped to elucidate its mechanisms in innate immunity. Furthermore, IAP has been shown to induce autophagy, highlighting its significant impact on gut health.
Article
Medicine, Research & Experimental
Yang Liu, Paul M. Cavallaro, Byeong-Moo Kim, Tao Liu, Hongyan Wang, Florian Kuehn, Fatemeh Adiliaghdam, Enyu Liu, Robin Vasan, Ehsan Samarbafzadeh, Matthew Z. Farber, Junhui Li, Meng Xu, Vidisha Mohad, Michael Choi, Richard A. Hodin
Summary: Liver fibrosis is associated with gut barrier dysfunction, and IAP has been identified as a potential therapy to prevent liver fibrosis. Oral IAP supplementation can protect the gut barrier and prevent liver fibrosis via a TLR4-mediated mechanism.
Article
Biochemistry & Molecular Biology
Rotimi Olusanya Arise, Olusola Mercy Adetiwa, Raphael Idowu Adeoye, Sylvia Omonirume Malomo
Summary: This study investigated the effects of sodium butyrate (SB) and taurine on rat intestinal alkaline phosphatase (RIA), as well as the interaction of taurine and/or SB with bacterial lipopolysaccharides on ALP activity. The results showed that taurine and SB enhanced the hydrolysis activity of RIA, especially in the presence of bacterial lipopolysaccharides. The study concluded that taurine and SB are activators of RIA and their positive synergistic interaction in the presence of bacterial lipopolysaccharides may play a role in attenuating bacterial LPS-mediated diseases.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Zhen Luo, Zeze Wang, Ping Li, Yulong Tan, Genlin He, Xiaoqian Liu, Tingting Shen, Xuesen Yang, Xue Luo
Summary: This study demonstrates that intestinal alkaline phosphatase (IAP) can improve intestinal barrier function and intestinal permeability by increasing intestinal tight junctions. It reduces systemic inflammation and multiple organ injury, ultimately improving the survival rate of heatstroke.
Article
Multidisciplinary Sciences
Yi Liu, Efren Reyes, David Castillo-Azofeifa, Ophir D. Klein, Todd Nystul, Diane L. Barber
Summary: Liu et al. find that intracellular pH dynamics regulate the secretory cell fate decision of intestinal stem cells. They observe an intracellular pH gradient in mouse small intestinal crypts, with the lowest pH in crypt stem cells and increasing along the crypt column. Disrupting this gradient inhibits crypt budding and Paneth cell differentiation, which can be rescued by exogenous WNT. They demonstrate that intracellular pH dynamics acts downstream of ATOH1, with increased pH promoting differentiation toward the secretory lineage. These findings establish a role for intracellular pH dynamics in cell fate decisions within an adult stem cell lineage.
NATURE COMMUNICATIONS
(2023)
Review
Microbiology
Gilberto Maia Santos, Shamila Ismael, Juliana Morais, Joao R. Araujo, Ana Faria, Conceicao Calhau, Claudia Marques
Summary: This article explores the important role of intestinal alkaline phosphatase (IALP) in leaky gut related diseases, discusses its function in maintaining intestinal homeostasis and barrier function, and explores the use of IALP as a biomarker and strategies for modulating its activity.
Article
Agriculture, Dairy & Animal Science
Wijesooriya Mudhiyanselage Nadeema Dissanayake, Malavige Romesha Chandanee, Sang-Myeong Lee, Jung Min Heo, Young-Joo Yi
Summary: This study investigated the role of intestinal alkaline phosphatase (IAP) in antibiotic-induced dysbiosis. The results showed that IAP activity decreased, RNA expression of tight junction proteins (TJP) decreased, and proinflammatory cytokine levels increased, which may affect intestinal integrity and function.
Article
Immunology
Sudha B. Singh, Cristina N. Coffman, Matthew G. Varga, Amanda Carroll-Portillo, Cody A. Braun, Henry C. Lin
Summary: Tight junctions are crucial for intestinal barrier integrity. This study reveals that Desulfovibrio vulgaris, a resident commensal bacterium, can contribute to leaky gut, providing new insights into the underlying mechanism of diseases associated with increased intestinal permeability.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Anku Guha, Mihir Sahoo, Khorsed Alam, D. Krishna Rao, Prasenjit Sen, Tharangattu N. Narayanan
Summary: In this study, the structural changes in water coordination in high alkaline electrolytes were investigated using experimental and density functional theory methods. The results showed that these structural changes could be a possible reason for the reduced catalytic activity of platinum electrodes in high pH environments.
Article
Chemistry, Analytical
Weiming Lin, Shuxia Lai, Dechan Lu, Qun Zhang, Xueliang Lin, Juqiang Lin, Jing Wang, Zufang Huang
Summary: Liquid-phase-based direct surface-enhanced Raman spectroscopy (SERS) has extensive applications in (bio)chemical analyses. However, existing techniques are generally constrained by signal fluctuations caused by the evaporation effect. This study proposes an acousto-assisted liquid-marble-based SERS platform, which achieves non-destructive and rapid coalescence of liquid marbles and high-sensitivity SERS detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Microbiology
Xiaojuan Tan, Xi Cheng, Jingjing Xiao, Qianqian Liu, Dongsheng Du, Minghui Li, Yang Sun, Jinwei Zhou, Guoping Zhu
Summary: Depletion of inorganic phosphate affects the virulence and biofilm formation of Pseudomonas aeruginosa. The deletion of the lapA gene leads to phenotypic changes and reduces the virulence of PAO1 in both a porcine skin model and Caenorhabditis elegans.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biophysics
Samy M. Shaban, Byeong-Seok Moon, Do-Gi Pyun, Dong-Hwan Kim
Summary: The study developed a colorimetric sensor based on the enzymatic activity of ALP and demonstrated a wider dynamic linear range and lower limit of detection through the quantitative assay of the enzymatic growth of AgNPs mediated by pAP.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Xiaolin Sheng, Dandan Liu, Sujani K. Gamage, Ying Luo, Emilie Viennois, Didier Merlin, Suri S. Iyer
Summary: The study found that the fecal IAP levels were significantly lower in mice with severe colitis compared to those with mild colitis. Mice treated with anti-TNF-Alpha showed significantly higher levels of IAP compared to untreated mice.
Article
Chemistry, Multidisciplinary
Mengting Lv, Huanan Li, Hua Cao, Teng Wang, Chengdian He, Yi Liang, Xiang Mao, Zhenyu Wang
Summary: This study presents a novel alkaline-responsive drug carrier, CS-GL, fabricated using a microfluidic approach with ultrasound assistance. The carrier exhibits high drug encapsulation efficiency and stable structure, enabling rapid drug release under both acidic and alkaline conditions.