Article
Neurosciences
Marco Manca, Piece Yen, Paolo Spaiardi, Giancarlo Russo, Roberta Giunta, Stuart L. Johnson, Walter Marcotti, Sergio Masetto
Summary: The residual I-Ca in both auditory and vestibular hair cells from Ca(V)1.3(-/-) mice was significantly reduced, but the K+ current profile changes in vestibular hair cells suggest that non-exocytotic transmission remains functional.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Neurosciences
Antonia Gonzalez-Garrido, Remy Pujol, Omar Lopez-Ramirez, Connor Finkbeiner, Ruth Anne Eatock, Jennifer S. Stone
Summary: Regenerated vestibular hair cells in adult mice show similarities to mature type II hair cells, but fail to fully recapture the properties of normal cells, displaying some characteristics of immature cells. Limitations in total cell number, cell type diversity, and extent of cellular differentiation suggest that interventions may be needed to promote full regeneration with potential for vestibular function recovery.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Adam J. Carlton, Jing-Yi Jeng, Fiorella C. Grandi, Francesca De Faveri, Federico Ceriani, Lara De Tomasi, Anna Underhill, Stuart L. Johnson, Kevin P. Legan, Corne J. Kros, Guy P. Richardson, Mirna Mustapha, Walter Marcotti
Summary: Sensory-independent Ca2+ spiking regulates the development of mammalian sensory systems. In immature cochlea, inner hair cells (IHCs) generate spontaneous Ca2+ action potentials (APs) either intrinsically or through intercellular Ca2+ waves. This study finds that intrinsic Ca2+ APs in IHCs, but not those induced by Ca2+ waves, play a role in the maturation and maintenance of hair bundles. By overexpressing the Kir2.1 potassium channel in IHCs, the study shows that absence of intrinsic Ca2+ APs leads to loss of mechanoelectrical transduction and upregulation of certain pathways related to morphogenesis and signaling in the Kir2.1-OE mice.
Article
Multidisciplinary Sciences
Zhenghong Bi, Xiang Li, Minhui Ren, Yunpeng Gu, Tong Zhu, Shuting Li, Guangqin Wang, Suhong Sun, Yuwei Sun, Zhiyong Liu
Summary: The study reveals the essential role of Tbx2 in the development of inner hair cells (IHCs) and suggests that co-upregulation of Tbx2 and Atoh1 in supporting cells can reprogram them into new IHCs with advanced differentiation status.
NATIONAL SCIENCE REVIEW
(2022)
Article
Allergy
Nicolas Giang, Marion Mars, Marc Moreau, Jose E. Mejia, Gregory Bouchaud, Antoine Magnan, Marine Michelet, Brice Ronsin, Geoffrey G. Murphy, Joerg Striessnig, Jean-Charles Guery, Lucette Pelletier, Magali Savignac
Summary: This study demonstrates that Ca(v)1.2 and Ca(v)1.3 in Th2 cells have non-redundant and synergistic functions, and deficiency in either one of these channels is sufficient to inhibit cardinal features of type 2 airway inflammation.
Article
Neurosciences
Jing-Yi Jeng, Adam J. Carlton, Stuart L. Johnson, Steve D. M. Brown, Matthew C. Holley, Michael R. Bowl, Walter Marcotti
Summary: The study compared age-related changes in inner hair cells (IHCs) in four mouse strains with different levels of progressive hearing loss. It found that the surface area of IHCs decreased with age, and the number of BK channels decreased in most mouse strains. The study also showed that the mechanoelectrical transducer (MET) current significantly decreased in mice with the Cdh23(ahl) allele, suggesting a direct contribution to age-related hearing loss progression.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Acoustics
Michael Faran, Miriam Furst
Summary: In recent years, experimental studies have shown that dysfunction of the inner-hair cells and their synapse to the auditory nerve is a significant contributor to hearing loss. This study presents a detailed biophysical model of the inner-hair cells in the auditory pathway, with the input as an acoustic signal and the output as the prediction of human audiometric thresholds. Different types of hearing loss were simulated by altering mechanical and biochemical parameters of the inner and outer hair cells. The results provide a common understanding of various hearing impairments.
JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Xiaolong Fu, Peifeng Wan, Ling Lu, Yingcui Wan, Ziyi Liu, Guodong Hong, Shengda Cao, Xiuli Bi, Jing Zhou, Ruifeng Qiao, Siwei Guo, Yu Xiao, Bingzheng Wang, Miao Chang, Wen Li, Peipei Li, Aizhen Zhang, Jin Sun, Renjie Chai, Jiangang Gao
Summary: In this study, two peroxisome-deficient mouse models were established to investigate the role of peroxisomes in hearing. The results showed that peroxisomes mainly function in the hair cells of cochleae and regulate BK channels, which are essential for auditory function. These findings suggest that BK channels could be potential targets for treating peroxisome-related hearing diseases.
Article
Neurosciences
Mitchell L. Heuermann, Sophia Matos, Deborah Hamilton, Brandon C. Cox
Summary: After insult, hair cells can regenerate from supporting cells within the first week of life. Regenerated cells express markers of both inner and outer hair cells and can form new synaptic connections. However, gene expression control is weakened in regenerated cells, leading to abnormal expression of hair cell types.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Hui Wang, Mingqi Xie, Giorgio Rizzi, Xin Li, Kelly Tan, Martin Fussenegger
Summary: Parkinson's disease (PD) is primarily caused by excessive activity-related Ca2+ oscillations, resulting in the selective loss of substantia nigra dopaminergic neurons. In this study, a synthetic-biology-inspired drug-discovery platform was used to identify potential PD drugs. Through virtual screening and deep-learning neural networks, the compound sclareol was identified as a promising candidate for PD pharmacotherapy. In vitro and in vivo experiments confirmed that sclareol selectively inhibits Ca(v)1.3, reduces the firing response of SNc DA neurons, and protects against DA neuronal loss and motor impairment in a mouse model of PD.
Article
Medicine, Research & Experimental
Nadine J. Ortner, Anupam Sah, Enrica Paradiso, Josef Shin, Strahinja Stojanovic, Niklas Hammer, Maria Haritonova, Nadja T. Hofer, Andrea Marcantoni, Laura Guarina, Petronel Tuluc, Tamara Theiner, Florian Pitterl, Karl Ebner, Herbert Oberacher, Emilio Carbone, Nadia Stefanova, Francesco Ferraguti, Nicolas Singewald, Jochen Roeper, Joerg Striessnig
Summary: Germline de novo missense variants of CACNA1D gene have been found to be associated with neurodevelopmental and endocrine dysfunction, demonstrating their disease-causing potential. In this study, mice carrying a specific CACNA1D variant were used to confirm its pathogenicity and provide insights into the underlying mechanism.
Article
Neurosciences
Ingrid Reichenberger, Claude J. Caussidier-Dechesne, Hans Straka
Summary: Calcium-binding protein calretinin is found in auditory but not vestibular afferent fibers in frogs, with differential distribution in sensory epithelia and ganglion cells. The absence of calretinin in vestibular ganglion cells is likely related to the lack of type I hair cells in anamniote vertebrates. The presence of calretinin in hair cells may play a role in encoding self-motion versus acoustic stimuli in frogs.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Kyung-Min Kwon, Myung-Jun Lee, Han-Saem Chung, Jae-Hong Pak, Chang-Jin Jeon
Summary: In this study, the organization of SST neurons in the Mongolian gerbil visual cortex was characterized using immunocytochemistry, quantitative analysis, and confocal microscopy. The results showed distinct features of SST neurons and provided some insight into their afferent circuitry in the gerbil VC. These findings may support future studies investigating the role of SST neurons in visual processing.
Article
Neurosciences
Lizbeth Grimaldo, Alejandro Sandoval, Paz Duran, Liliana Gomez Flores-Ramos, Ricardo Felix
Summary: This study investigates the degradation mechanisms of neuronal CaV1.3 channels by Parkin and suggests that Parkin promotes proteasomal degradation of CaV1.3, which may be relevant to the pathophysiology of Parkinson's disease.
JOURNAL OF NEUROPHYSIOLOGY
(2022)
Article
Neurosciences
Snezana Levic
Summary: This study investigated the functional expression of calcium-sensitive potassium currents and their role in spontaneous electrical activity in developing and regenerating hair cells in chick basilar papilla. The results showed that the calcium-sensitive potassium currents were downregulated during development and were highly sensitive to intracellular calcium. Additionally, these currents participate in the encoding of spontaneous electrical activity in hair cells.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)