Article
Multidisciplinary Sciences
Haohao Wu, Charles Petitpre, Paula Fontanet, Anil Sharma, Carmelo Bellardita, Rolen M. Quadros, Paulo R. Jannig, Yiqiao Wang, J. Alexander Heimel, Kylie K. Y. Cheung, Simone Wanderoy, Yang Xuan, Konstantinos Meletis, Jorge Ruas, Channabasavaiah B. Gurumurthy, Ole Kiehn, Saida Hadjab, Francois Lallemend
Summary: The study identified three main types of proprioceptive neurons (Ia, Ib and II PNs) and found that they segregate into eight distinct subgroups. Importantly, Ia-PN subtypes are plastic, suggesting a role in adaptive proprioception during motor behavior.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Qin Zheng, Wenrui Xie, Debora D. Luckemeyer, Mark Lay, Xue-Wei Wang, Xinzhong Dong, Nathachit Limjunyawong, Yaqing Ye, Feng-Quan Zhou, Judith A. Strong, Jun-Ming Zhang, Xintong Dong
Summary: This article explores the mechanisms underlying spontaneous pain and identifies a unique form of abnormal spontaneous activity. The study shows that this activity is triggered by sympathetic nerves that grow into ganglia after injury and is correlated with spontaneous pain behaviors. The findings contribute to the development of new treatments for spontaneous pain.
Article
Multidisciplinary Sciences
Caroline Siebald, Philippe F. Y. Vincent, Riley T. Bottom, Shuohao Sun, Daniel O. J. Reijntjes, Marco Manca, Elisabeth Glowatzki, Ulrich Muller
Summary: This study reveals three molecularly defined subtypes of type I SGNs using single-cell RNA sequencing technology, but the extent to which they correspond to physiological subtypes is unclear. Using genetically marked SGN subtypes, the researchers found that Lypd1-CreERT2 expressing SGNs have a low SR and preferentially innervate the IHC modiolar side, while Calb2-CreERT2 expressing SGNs have a wider range of SRs and preferentially innervate the IHC pillar side.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
Ran Yang, Shanzi Yu, Lu Chen, Jiahui Ma, Zhenxing Li, Dong Huang
Summary: This study aimed to investigate the role and mechanism of action of Alkbh5 on trigeminal neuralgia. The results showed that the relative expression level of Alkbh5 was negatively correlated with the mechanical pain threshold in rats. Alkbh5 alleviated the mechanical hyperalgesia of the trigeminal neuralgia rat model by negatively regulating the expression of p-ERK.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Neurosciences
Shilin Jia, JinYue Liu, Yanhao Chu, Qing Liu, Lijia Mai, Wenguo Fan
Summary: The trigeminal ganglion is responsible for processing orofacial sensory information, and the communication between trigeminal ganglion neurons and other cells plays a crucial role in this process. Purinergic signaling, a widespread pathway in intercellular communication, contributes to neuromodulation and orofacial sensation. In this study, single-cell RNA sequencing was performed to identify different cell populations in the trigeminal ganglion and explore the expression patterns of genes associated with purinergic signaling.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Medicine, Research & Experimental
Yunmei Zhang, Yuan Zhang, Zizhang Wang, Yufang Sun, Xinghong Jiang, Man Xue, Yafeng Yu, Jin Tao
Summary: This study reveals that gentamicin decreases the I-DR in VG neurons through JNK and PKA-mediated signaling pathways, leading to neuronal hyperexcitability. Blocking JNK signaling and I-DR can attenuate this effect.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Engineering, Biomedical
M. Piotrkiewicz
Summary: Investigating MN physiology with computer simulations allows evaluation of human MNs' biophysical properties and understanding the role of PICs in shaping MU discharge patterns. Recent MN models focus on PICs and their interaction with synaptic inputs to produce various discharge patterns.
BIOCYBERNETICS AND BIOMEDICAL ENGINEERING
(2021)
Article
Clinical Neurology
Jiaomei Zhao, Yue Zhang, Xiangbo Liu, Yong Rao, Jia Fu, Lu Hua, Cehua Ou
Summary: The study found that the SK3 channel is expressed in the trigeminal ganglion of rats and has an impact on pain thresholds in trigeminal neuralgia. Activation of the SK3 channel can increase pain thresholds, while antagonists can decrease them.
NEUROLOGICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Lina A. Matschke, Marlene A. Komadowski, Annette Stoehr, Bolam Lee, Martin T. Henrich, Markus Griesbach, Susanne Rinne, Fanni F. Geibl, Wei-Hua Chiu, James B. Koprich, Jonathan M. Brotchie, Aytug K. Kiper, Amalia M. Dolga, Wolfgang H. Oertel, Niels Decher
Summary: This study characterizes the electrophysiological properties of neurons in Parkinson's disease (PD) models and suggests a role of Ca2+-activated K+ (SK) channels in PD by linking changes in LC firing rate to SK channel dysfunction.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Gehong Dong, Xuxiang Liu, Lifu Wang, Wenjuan Yin, Alyssa Bouska, Qiang Gong, Kunal Shetty, Lu Chen, Sunandini Sharma, Jibin Zhang, Carmen Lome-Maldonado, Leticia Quintanilla-Martinez, Yuping Li, Joo Y. Song, Wenyan Zhang, Yunfei Shi, Jinhui Wang, Lingbo Kong, Xiwei Wu, Jingwen Wang, Hong-Gang Liu, Lingfei Kong, Wenyong Sun, Weiping Liu, Lili Wang, Timothy W. McKeithan, Javeed Iqbal, Wing C. Chan
Summary: This study provides a comprehensive genomic analysis and clinical study of extra-nodal NK/T-cell lymphoma, nasal type (ENKTCL). It identifies factors associated with overall survival, reveals frequent oncogenic activation of the JAK/STAT3 pathway, and identifies genetic clusters associated with different clinical outcomes. The study also highlights a novel functional cooperation between activating STAT3 mutations and loss of the TSG, PRDM1.
Article
Neurosciences
Timothy OIsen, Alberto Capurro, Masa Svent, Nadia Pilati, Charles Large, Nick Hartell, Martine Hamann
Summary: Spontaneous subthreshold activity in the central nervous system plays a crucial role in processing and transmitting information, especially at auditory synapses. Excessive spontaneous activity can lead to auditory disorders. Positive modulation of Kv3 K+ currents can reduce synaptic bombardment onto DCN principal fusiform cells. Reducing synaptic noise through pre-synaptic modulation could be a potential therapeutic target for hearing disorders.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Cardiac & Cardiovascular Systems
Rui Song, Xiang-Qun Hu, Monica Romero, Mark A. Holguin, Whitney Kagabo, Daliao Xiao, Sean M. Wilson, Lubo Zhang
Summary: Pregnancy increases the association of BKCa channel subunits and alters the localization of RyR subtypes in uterine arteries. Knockdown of RyR1 or RyR2 decreases Ca2+ sparks and STOCs frequency, increases myogenic tone, while RyR3 knockdown has no significant effect. This study provides new insights into the mechanisms of uterine vascular adaptation to pregnancy through RyR subtypes.
CARDIOVASCULAR RESEARCH
(2021)
Article
Genetics & Heredity
Harini V. Gudiseva, Vrathasha Vrathasha, Jie He, Devesh Bungatavula, Joan M. O'Brien, Venkata R. M. Chavali
Summary: This study aimed to identify marker genes with differential gene expression and RGC subtypes in cultures of human-induced pluripotent stem cell (iPSC)-derived retinal ganglion cells. Single-cell sequencing was performed and cluster analyses were conducted to determine the RGC diversity and subtype specification within iPSC-RGCs. DEG analysis revealed subsets of RGCs with specific markers and significant differential expression associated with various biological processes and pathways.
Article
Neurosciences
Ryan J. Vaden, Jianguo G. Gu
Summary: This study characterizes the electrophysiological properties of trigeminal A beta-afferent neurons and investigates their mechanical and chemical sensitivity. The results show that different subtypes of these neurons respond differently to mechanical stimulation and sensory mediators.
Article
Physiology
Andrea Marcantoni, Giuseppe Chiantia, Giulia Tomagra, Enis Hidisoglu, Claudio Franchino, Valentina Carabelli, Emilio Carbone
Summary: In this study, we used microelectrode arrays to record spontaneous extracellular action potentials from rat chromaffin cells and compared them with intracellularly recorded action potentials. We found two distinct firing modes and observed that eAPs are regulated by different ion channels.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Review
Neurosciences
Luigi Sforna, Alfredo Megaro, Mauro Pessia, Fabio Franciolini, Luigi Catacuzzeno
CURRENT NEUROPHARMACOLOGY
(2018)
Article
Cell Biology
Paolo Rosa, Luigi Catacuzzeno, Luigi Sforna, Giorgio Mangino, Silvia Carlomagno, Gabriella Mincione, Vincenzo Petrozza, Giuseppe Ragona, Fabio Franciolini, Antonella Calogero
JOURNAL OF CELLULAR PHYSIOLOGY
(2018)
Article
Neurosciences
Carmen Mecca, Ileana Giambanco, Stefano Bruscoli, Oxana Bereshchenko, Bernard Fioretti, Carlo Riccardi, Rosario Donato, Cataldo Arcuri
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Maria Ricci, Francesco Ragonese, Beatrice Gironi, Marco Paolantoni, Assunta Morresi, Loredana Latterini, Bernard Fioretti, Paola Sassi
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Luigi Sforna, Fabio Franciolini, Luigi Catacuzzeno
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Nutrition & Dietetics
Roberto Spogli, Maria Bastianini, Francesco Ragonese, Rossana Giulietta Iannitti, Lorenzo Monarca, Federica Bastioli, Irina Nakashidze, Gabriele Brecchia, Laura Menchetti, Michela Codini, Cataldo Arcuri, Loretta Mancinelli, Bernard Fioretti
Article
Chemistry, Multidisciplinary
Cataldo Arcuri, Lorenzo Monarca, Francesco Ragonese, Carmen Mecca, Stefano Bruscoli, Stefano Giovagnoli, Rosario Donato, Oxana Bereshchenko, Bernard Fioretti, Ferdinando Costantino
Article
Agriculture, Dairy & Animal Science
L. Menchetti, L. Vecchione, I Filipescu, V. F. Petrescu, B. Fioretti, T. Beccari, M. R. Ceccarini, M. Codini, A. Quattrone, M. Trabalza-Marinucci, O. Barbato, G. Brecchia
Review
Physiology
Luigi Catacuzzeno, Luigi Sforna, Fabio Franciolini
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2020)
Article
Physiology
Luigi Catacuzzeno, Luigi Sforna, Fabio Franciolini, Robert S. Eisenberg
Summary: The generation of action potentials in excitable cells relies on the activation kinetics of voltage-gated Na (Na-V) and K (K-V) channels, with Na-V channels activating much faster. Recent experimental evidence shows that the difference in activation kinetics is due to the presence of a highly conserved threonine in Na-V channels and isoleucine in K-V channels within the gating pore of the voltage sensor domain. Through multiscale modeling, it has been determined that the faster activation kinetics of Na-V channels is attributed to a stronger dielectric polarization effect by threonine compared to isoleucine.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Biophysics
Luigi Catacuzzeno, Fabio Franciolini, Francisco Bezanilla, Robert S. Eisenberg
Summary: The activation of voltage-dependent ion channels is related to the movement of gating charges and gating currents. Analysis of fluctuations in macroscopic gating currents from a population of channels allows estimation of their magnitude. A mathematical model suggests that in real channels, the voltage sensor moves through a sequence of intermediate states, leading to frequent crossings of gating charges.
BIOPHYSICAL JOURNAL
(2021)
Article
Cell Biology
Luigi Sforna, Antonio Michelucci, Francesco Morena, Chiara Argentati, Fabio Franciolini, Massimo Vassalli, Sabata Martino, Luigi Catacuzzeno
Summary: Regulatory volume decrease (RVD) is critical in controlling cell functions like migration, and is mainly sustained by swelling-activated Cl- current (I-Cl,I-swell) modulated by cytoplasmic Ca2+. Piezo1 controls RVD and migration in HEK293T cells by modulating I-Cl,I-swell through Ca2+ influx, with different expression levels affecting cell migration ability.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Neurosciences
Luigi Catacuzzeno, Fabio Franciolini
Summary: This retrospective examines the voltage-sensing mechanisms and gating models of ion channels. It traces the history from the postulation of charged gating particles by Hodgkin and Huxley in 1952 to the discovery of gating currents 20 years later. In the early 1980s, the common architecture of voltage-dependent channels was identified, with the fourth segment (S4) serving as the voltage sensor. The development of the sliding helix model and the analysis of X-ray crystallographic structures have furthered our understanding of channel gating mechanisms.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Cell Biology
Antonio Michelucci, Luigi Sforna, Angela Di Battista, Fabio Franciolini, Luigi Catacuzzeno
Summary: Glioblastoma (GBM) is a deadly brain tumor that invades healthy brain tissue through cell migration and invasion. The precise nature of the K+ channels involved in cell volume regulation in this type of tumor has remained uncertain. This study found that hypotonic-induced cell swelling in GBM U87-MG cells triggers the opening of calcium-activated K+ channels, particularly BKCa and IKCa, which play a key role in the regulatory volume decrease following hypotonic shock.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Review
Physiology
Luigi Catacuzzeno, Franco Conti, Fabio Franciolini
Summary: This article reviews the retrospective on gating currents from their first recording in 1973 to the present day and their contribution to understanding channel gating. The past 50 years have witnessed great progress in elucidating the mechanisms of channel gating through the recording and analysis of gating currents. The review covers the development of the concept of gating particles and gating currents, early studies on specific channels, expansion to other channels and non-channel structures, and the translation of gating-charge/voltage-sensor movements into pore opening and associated pathologies.
JOURNAL OF GENERAL PHYSIOLOGY
(2023)