Article
Chemistry, Analytical
Pengfei Zhang, Rui Wang, Zijian Wan, Xinyu Zhou, Guangzhong Ma, Jayeeta Kolay, Jiapei Jiang, Shaopeng Wang
Summary: The article introduces a total internal reflection-based evanescent scattering microscopy technique that can detect single proteins without labeling and provides a high-precision measurement method for protein binding kinetics.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Soocheol Kim, Jin Hwa Ryu, Hoesung Yang, Kyuwon Han, Hyunseok Kim, Kwangsoo Cho, Soyoung Park, Sang Gi Hong, Kangbok Lee
Summary: This paper presents a spectrometer-based surface plasmon resonance imaging (SPRi) technique without mechanical scanning. A polarized broadband light source illuminates an object via a gold-coated prism, and the reflected light is spatially modulated by a digital mirror device (DMD) and measured with a spectrometer. Reflectance spectral images are reconstructed using the Hadamard transform (HT), and a refractive index (RI) map is visualized by analyzing the resonance peak shift at each image pixel. The method is demonstrated to have high resolution, sensitivity, and dynamic detection range, with experimental results validating the simulations.
Article
Chemistry, Analytical
Prerit Mathur, Anna Fomitcheva Khartchenko, Stavros Stavrakis, Govind V. Kaigala, Andrew J. deMello
Summary: We developed a method for monitoring localized antigen-antibody binding on cell and tissue sections using fluorescence lifetime imaging. By measuring the difference in fluorescence decay times of tagged antibodies in free solution and in the bound state, we can track the binding fraction over time and determine the binding kinetics. This method allows us to measure binding constants and observe biomarker expression effects on kinetics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
F. Mousseau, C. Feraudet Tarisse, S. Simon, T. Gacoin, A. Alexandrou, C. I. Bouzigues
Summary: The use of lanthanide-doped nanoparticles as luminescent LFA probes offers higher sensitivity and measurement accuracy for detecting various biomolecules, including enterotoxins. Compared to gold nanoparticle-based LFAs, this method shows at least an order of magnitude improvement in analytical sensitivity and enables simultaneous quantitative detection of multiple biological toxins.
Article
Chemistry, Analytical
Edwin J. Ortiz-Riano, Diana L. Mancera-Zapata, Martha Ulloa-Ramirez, Fernando Arce-Vega, Eden Morales-Narvaez
Summary: Macromolecular association is important in biomedical sciences. This study presents a real-time biosensing platform using graphene oxide-modified microwell plates and fluorophore-labeled proteins to determine protein-binding constants. The platform proves to be comparable and consistent with existing methods in determining protein kinetics constants.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Yunshan Fan, Yara Aceta, Esther Hessong, Athena Bengston, Laris A. Biageyian, Quan P. Huynh, Robert M. Corn
Summary: Diffraction patterns observed in SPRI microscopy measurements of single gold nanorods exhibit complex behavior near the longitudinal plasmonic resonance band, with a unitless ratio parameter M-R created to describe the diffraction pattern distribution. Results show that this behavior only occurs in the anomalous dispersion region, when the laser wavelength used is shorter than the AuNR plasmonic resonance maxima.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Analytical
Lukas Wunderlich, Peter Hausler, Susanne Maerkl, Rudolf Bierl, Thomas Hirsch
Summary: A label-free sensor based on SPR imaging was proposed to monitor nanoparticles in fresh water, with improved sensitivity by enhancing the detector and using LED excitation for the light source. Different self-assembled monolayers were screened as receptors, with the best system being a dodecanethiol monolayer on the gold sensor surface. The sensor successfully detected lanthanide-doped nanoparticles of 29 nm in water.
Article
Biochemical Research Methods
Anik Mazumder, Mohammad Mozammal, Muhammad Anisuzzaman Talukder
Summary: Cell imaging is crucial for biomedical and microbiology studies, but three-dimensional imaging is challenging and requires expensive and complex equipment. This study proposes a low-cost imaging technique using surface plasmon coupled emission patterns to accurately image cells.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Optics
Yong Wei, Chen Shi, Chunlan Liu, Chunbiao Liu, Xingkai Wang, Yixiong Tang, Rui Wang, Zhihai Liu
Summary: In this paper, a 3D displacement sensor based on fiber SPR is proposed to measure micro displacement in X, Y and Z axes.
Article
Optics
Shu-cheng Lo, Chia-wei Lee, Ruey-lin Chern, Pei-kuen Wei
Summary: In this work, high-performance surface plasmonic sensors using gold nanostructures and Bragg photonic structures are presented. The hybrid Tamm-plasmon mode, resulting from the Fano coupling between higher order Tatum plasmon states and Bloch-wave surface plasmon polariton on the gold nanoslit array, demonstrates high surface sensitivity and resonant quality. The experimental results show that the hybrid mode has significantly higher sensitivity and better signal-to-noise ratios in biomolecular interaction measurement.
Article
Biochemical Research Methods
Youjun Zeng, Zhaogang Nie, Dongyun Kai, Jiajie Chen, Yonghong Shao, Weifu Kong, Zhengqiang Yuan, Ho-Pui Ho, Fangteng Zhang
Summary: This paper presents a novel WSPRi sensing system with a high anti-interference capability and low cost. By introducing devices such as an acousto-optic tunable filter and a halogen lamp, real-time recording of brine concentration and formation of the coffee ring effect were successfully achieved, and the performance of the biosensor was improved. The high anti-interference capability and accuracy of this system will greatly contribute to the exploration of biomolecule behaviors in biological and biochemistry studies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Chun-Yuan Wang, Yungang Sang, Xinyue Yang, Soniya S. Raja, Chang-Wei Cheng, Haozhi Li, Yufeng Ding, Shuoyan Sun, Hyeyoung Ahn, Chih-Kang Shih, Shangjr Gwo, Jinwei Shi
Summary: This study demonstrates a strong coupling system achieved by coupling localized surface plasmon modes. Under specific conditions, a root N scaling of Rabi splitting energy is observed, along with a confirmed giant Rabi splitting in the visible spectral range. Additionally, the coupling strength reaches the ultrastrong coupling regime in some cases, representing about 10% of the mode energy.
Article
Chemistry, Multidisciplinary
Yu Chen, Meng Wang, Kai Zheng, Yaguang Ren, Hao Xu, Zhongzheng Yu, Feifan Zhou, Chengbo Liu, Junle Qu, Jun Song
Summary: This study presents an effective strategy to synthesize antimony nanopolyhedrons and successfully observes the modulation effect of different morphologies on their localized surface plasmon resonance in experimentation. Antimony nanopolyhedrons exhibit excellent performance in PTCE and good photothermal stability, as well as biocompatibility. The research also demonstrates the construction of multifunctional nanomedicines based on antimony nanopolyhedrons for efficient photoacoustic-imaging-guided synergistic photothermal/immune-therapy of tumors in vivo.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Analytical
Martina Vrablova, Katerina Smutna, Ivan Koutnik, Tomas Prostejovsky, Radim Zebrak
Summary: Glyphosate is a widely used pesticide. In order to tackle the issue of glyphosate removal, photocatalysis technology and a new sensor approach have been introduced. The aim of the research was to design and test an SPRi biosensor suitable for glyphosate degradation and the experimental results were recorded.
Article
Chemistry, Multidisciplinary
Yi-Nan Liu, Zhen-Ting Lv, Wen-Li Lv, Dong-Feng Liu, Xian-Wei Liu
Summary: We developed a label-free and noninvasive imaging method to measure electron transfer in microbial cells. By imaging the redox reaction of cytochromes in microbial cells, we obtained the electrochemical activity parameters of individual cells, revealing heterogeneities in electron transfer at the single-cell level.
Article
Engineering, Electrical & Electronic
Yisbel Eloisa Marin, Veronica Toccafondo, Philippe Velha, Simona Scarano, Stefano Tirelli, Alessandro Nottola, Yoonah Jeong, Hyunpyo Jeon, Sanghun Kim, Maria Minunni, Fabrizio Di Pasquale, Claudio J. Oton
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2019)
Article
Biophysics
Roberta D'Agata, Noemi Bellassai, Matteo Allegretti, Andrea Rozzi, Sasa Korom, Alex Manicardi, Elisa Melucci, Edoardo Pescarmona, Roberto Corradini, Patrizio Giacomini, Giuseppe Spoto
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Analytical
Roberta D'Agata, Noemi Bellassai, Maria Chiara Giuffrida, Angela Margherita Aura, Christian Petri, Peter Kogler, Graziella Vecchio, Ulrich Jonas, Giuseppe Spoto
Summary: Surface plasmon resonance (SPR) is widely used for detecting biomolecular systems, but faces challenges when analyzing blood plasma or serum samples due to non-specific adsorption. Traditional polymeric layers for antifouling may not be fully effective for SPR detection due to limitations in layer thickness. A new pentrimeric system is proposed for sensitive SPR detection with less than 2 nm thickness, showing potential for improving antifouling properties in molecular diagnostics.
Article
Biophysics
Meenu Selvaraj, Pierpaolo Greco, Matteo Sensi, Gulseren Deniz Saygin, Noemi Bellassai, Roberta D'Agata, Giuseppe Spoto, Fabio Biscarini
Summary: A dual gate/common channel organic transistor architecture was designed for quantifying the concentration of one of the strands of miRNA-21 in solution, showing high sensitivity and low detection limit. The dose curves were described using an analytical function derived from a thermodynamic model, indicating the interplay of different equilibria. The lower binding free energy characteristic of hybridization on the device surface compared to reaction in solution suggests a partially inhibiting effect of the surface and presence of competing reactions.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemical Research Methods
Noemi Bellassai, Roberta D'Agata, Giuseppe Spoto
Summary: Nucleic acid nanotechnology focuses on designing and developing synthetic nucleic acid strands to create nanosized functional systems. The structural properties and conformational polymorphism of nucleic acid sequences make nucleic acid nanostructures attractive in biosensing applications. Recent advances in biosensing have been derived from molecular beacon and DNA origami structures, each offering unique characteristics and potentials in the field.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Polymer Science
Roberta D'Agata, Noemi Bellassai, Vanessa Jungbluth, Giuseppe Spoto
Summary: Strategies for developing antifouling surface coatings are essential for surface plasmon resonance (SPR) sensing in various analytical fields, such as clinical diagnostics and food quality control. This review emphasizes the importance of using appropriate antifouling materials to achieve excellent biosensing performance and highlights progress in overcoming challenges related to target detection in complex samples.
Article
Chemistry, Multidisciplinary
Noemi Bellassai, Roberta D'Agata, Almudena Marti, Andrea Rozzi, Stefano Volpi, Matteo Allegretti, Roberto Corradini, Patrizio Giacomini, Jurriaan Huskens, Giuseppe Spoto
Summary: A new nanoparticle-enhanced assay simplifies the detection of tumor DNA in patient plasma, while a dual-functional low-fouling PLL-based surface layer allows for the detection of mutated DNA at attomolar levels without PCR amplification.
Article
Chemistry, Analytical
Marzia Calcagno, Roberta D'Agata, Giulia Breveglieri, Monica Borgatti, Noemi Bellassai, Roberto Gambari, Giuseppe Spoto
Summary: This study presents a nanoparticle-enhanced strategy based on surface plasmon resonance imaging (SPRI) for non-invasive prenatal fetal sex determination, allowing for detection of male cell-free fetal DNA without the need for preliminary amplification of DNA target sequence. The developed protocol successfully reveals male cell-free fetal DNA in the plasma of pregnant women at different gestational ages, including early gestational ages. This approach could pave the way for faster and cost-effective non-invasive prenatal testing.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Francesca Torrini, Lapo Renai, Simona Scarano, Massimo Del Bubba, Pasquale Palladino, Maria Minunni
Summary: This study examined the color development of common anthocyanins and anthocyanin-rich extracts using zinc-anthocyanin complexes as molecular probes. It was found that the absorbance in the blue region increased in the presence of excess zinc ions at acidic pH for certain derivatives, possibly due to base stabilization. The assay conditions were applied to natural extracts and demonstrated the effectiveness of this colorimetric method for quantifying anthocyanins.
Article
Chemistry, Analytical
Federica Battaglia, Francesca Bonelli, Micaela Sgorbini, Luigi Intorre, Maria Minunni, Simona Scarano, Valentina Meucci
Summary: In this study, a new sandwich-type immunoenzymatic assay based on a molecularly imprinted polymer (MIP) was developed for the determination of procalcitonin (PCT) in veterinary species. This method offers a promising analytical tool for the detection of disease biomarkers in veterinary patients, where validated assays are currently lacking.
ANALYTICAL METHODS
(2022)
Article
Biophysics
Francesca Torrini, Federica Battaglia, Pasquale Palladino, Simona Scarano, Maria Minunni
Summary: This study reports the development of a novel mimetic based on polynorepinephrine for IgG detection using label-free and real-time Surface Plasmon Resonance (SPR) sensing. The designed platform exhibited excellent analytical performances and stability for detecting IgG antibodies, making it a promising tool for serological point-of-care testing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Mariagrazia Lettieri, Michele Spinelli, Laura Caponi, Simona Scarano, Pasquale Palladino, Angela Amoresano, Maria Minunni
Summary: We developed a simple colorimetric assay for catecholamine detection in human urine, demonstrating the time-dependent formation and molecular weight of melanochrome (MC) and 5,6-indolequinone (IQ) derived from levodopa (LD), dopamine (DA), and norepinephrine (NE) oxidation. This assay can quantitatively detect LD and DA in urine, showing potential applicability in therapeutic drug monitoring and clinical chemistry.
Article
Chemistry, Analytical
Mariagrazia Lettieri, Simona Scarano, Laura Caponi, Andrea Bertolini, Alessandro Saba, Pasquale Palladino, Maria Minunni
Summary: We developed a simple and low-cost assay for copper in urine using a serotonin-derived fluorophore. The assay showed good reproducibility and low detection limits in buffer and artificial urine, and demonstrated excellent analytical performances in human urine samples. This is the first example of copper ion detection exploiting the fluorescence quenching of a biopolymer, offering a potential diagnostic tool for copper-dependent diseases.
Article
Biotechnology & Applied Microbiology
Mariagrazia Lettieri, Pasquale Palladino, Simona Scarano, Maria Minunni
Summary: CuNCs offer advantages such as stability, ease of fabrication, affordability, unique photoluminescent properties, and high biocompatibility, making them a promising nanomaterial for various detection strategies. They demonstrate high sensitivity in different detection platforms, particularly in complex biological matrices.
SENSORS AND ACTUATORS REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Gina Elena Giacomazzo, Pasquale Palladino, Cristina Gellini, Gianluca Salerno, Veronica Baldoneschi, Alessandro Feis, Simona Scarano, Maria Minunni, Barbara Richichi