Article
Chemistry, Multidisciplinary
A. R. Ortiz Moreno, R. Li, K. Wu, R. Schirhagl
Summary: Lipid peroxidation is a process where reactive oxygen species attack lipids within cell membranes, leading to toxic and carcinogenic products. Understanding this process at nanoscale resolution is important and challenging due to the short lifespan and reactivity of the radical intermediates involved. In this study, lipid peroxidation of three artificial lipid bilayers on a diamond substrate was investigated using a diamond quantum sensing method called T-1-relaxometry, allowing for in situ measurements and imaging of radical intermediates.
Article
Chemistry, Multidisciplinary
Rashad Kariuki, Rowan Penman, Saffron J. Bryant, Rebecca Orrell-Trigg, Nastaran Meftahi, Russell J. Crawford, Chris F. McConville, Gary Bryant, Kislon Voitchovsky, Charlotte E. Conn, Andrew J. Christofferson, Aaron Elbourne
Summary: Nanomaterials have the potential to revolutionize biological and biomedical research, and this study provides detailed insights into how gold nanoparticles interact with phospholipid membranes. The study shows that the nanoparticles can be absorbed and internalized by fluid-phase membranes, and partially embed into gel-phase membranes, causing perturbations throughout the bilayers.
Article
Polymer Science
Che-Lun Chin, Lu-Jan Huang, Zheng-Xian Lu, Wei-Chun Weng, Ling Chao
Summary: This study demonstrates that the water absorption ability of dried PAAm hydrogel can facilitate the formation of hydrogel-SLBs. The high quality of the hydrogel surface is crucial for SLB formation, and minimizing oxygen exposure during drying can prevent the formation of an oxidized crust.
Article
Chemistry, Physical
Niki Baccile, Anyssa Derj, Cedric Boissiere, Vincent Humblot, Ariane Deniset-Besseau
Summary: The development of supported glycosylated lipid layers using microbial biosurfactants provides a versatile and robust method for preparing defect-free and homogeneous lipid monolayers. The method offers precise control of thickness below 10 nm and is compatible with aqueous solutions. Characterization techniques show that the glycosylated SLM are uniformly thick at 2.8 +/- 1.0 nm and defect-free at nanometer to centimeter scales.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Multidisciplinary
Hui Yin, Arielle C. Mensch, Christian A. Lochbaum, Isabel U. Foreman-Ortiz, Emily R. Caudill, Robert J. Hamers, Joel A. Pedersen
Summary: Supported lipid bilayers are important model systems for studying the interactions of proteins, peptides, and nanoparticles with biological membranes. The properties of the solid substrate, such as topography and coating, can affect the formation and properties of supported phospholipid bilayers. The interactions of nanoparticles with supported lipid bilayers depend on the substrate composition.
Article
Chemistry, Physical
Alessandra Luchini, Federica Sebastiani, Frederik Gronbaek Tidemand, Krishna Chaithanya Batchu, Mario Campana, Giovanna Fragneto, Marite Cardenas, Lise Arleth
Summary: Peptide discs, resembling 10 nm discoidal lipid bilayers, can be used as precursors for Supported Lipid Bilayers (SLBs) without the need to alter any experimental variables, allowing for faster formation compared to vesicle fusion. SLBs can be successfully formed from both synthetic and natural lipid mixtures, with traces of 18A peptide at the support surface having no effect on the SLB structure.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Myrto Kyropoulou, Saziye Yorulmaz Avsar, Cora-Ann Schoenenberger, Cornelia G. Palivan, Wolfgang. P. Meier
Summary: The study presents a refined and efficient method to produce solid supported polymer membranes based on amphiphlic triblock copolymers through vesicle fusion. Factors influencing the characteristics of polymer assemblies were explored, with the proper combination leading to uniform planar membranes. This approach brings many advantages to the development of synthetic planar membranes for bio-sensing and biotechnological applications.
Article
Physics, Multidisciplinary
Shi Yu, Jianqiao Zhao, Ruizhi Chu, Xiao Li, Guoguang Wu, Xianliang Meng
Summary: This study presents mesoscale models for the anomalous diffusion of a polymer chain on a heterogeneous surface. The simulation results show that bead-spring chains exhibit sub-diffusion on charged lipid bilayers, while a simulated 17 base pairs double stranded DNA exhibits normal diffusion. The energy landscape experienced by short DNA chains is less heterogeneous compared to long DNA chains, resulting in different diffusion behaviors.
Article
Chemistry, Analytical
Agnieszka Wieckowska, Elzbieta Jablonowska, Marcin Jaskolowski, Renata Bilewicz
Summary: Metal nanostructures are commonly used in thin films to enhance the conductivity of electrodes. In this study, we show that even at very low surface concentrations, gold clusters act as remote electrodes, facilitating electron transfer to solution species when embedded in a lipid film. The presence of gold clusters enables efficient electron transfer through the lipid barrier, as demonstrated by the electrode processes of hexaammineruthenium(III). Reversibility of the electrochemical probe is restored with the addition of as low as 0.01 mol% of Au clusters dispersed in the outer leaflet of the lipid bilayer.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Claudia Lubrano, Ugo Bruno, Chiara Ausilio, Francesca Santoro
Summary: This study investigates the short-term depression of a biomembrane-based organic electrochemical transistor (OECT) through supported lipid bilayer mediation. The ionic barrier behavior of the lipid bilayer is utilized to enhance the neuromorphic operation of the OECT. These biomimetic neuromorphic devices pave the way for in vitro platforms resembling synapses to study and characterize synaptic plasticity loss in neurodegenerative diseases.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Physical
Avijit Maity, Soumya Kanti De, Anjan Chakraborty
Summary: The coating of proteins and lipids around nanoparticles, known as protein and lipid coronas, have promising biomedical applications. The formation of a lipid corona is a relatively new field, where high lipid concentration leads to unique lipid corona formation and low lipid concentration induces aggregation. The interaction between lipids and nanoparticles is time-dependent and influenced by surface charge.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Multidisciplinary
He Gao, Pei Wu, Pei Song, Bin Kang, Jing-Juan Xu, Hong-Yuan Chen
Summary: Plasmonic nanoparticles like gold and silver have potential as nanoprobes for biological sensing and imaging, with a demand for small gold nanoparticles (Au NPs) comparable in size to quantum dots and fluorescent proteins. Very small nanoparticles below the Rayleigh limit are difficult to visualize using light scattering with a dark-field microscope, and while a photothermal microscope can detect them, its imaging speed is too slow for live cell processes. The absorption modulated scattering microscopy (AMSM) method allows for imaging sub-10 nm Au NPs within a cellular medium, offering sensitivity similar to photothermal microscopy but with a much faster imaging speed suitable for dynamic tracking of small nanoparticles in living cells.
Review
Nanoscience & Nanotechnology
Andrea Mescola, Gregorio Ragazzini, Paolo Facci, Andrea Alessandrini
Summary: Antimicrobial peptides and lipopeptides are promising molecules against resistant bacterial infections, with broad-spectrum activity and rapid bactericidal action. They act on the bacterial membrane, disrupting its structure and permeability, through physical interactions that make it difficult for the bacteria to develop resistance. By understanding their mechanism of action, new synthetic peptides can be developed to enhance bactericidal activity. Atomic force microscopy provides a valuable technique to study the effects of antimicrobial peptides and lipopeptides on lipid bilayers, and can be used alongside fluorescence microscopy to investigate different lipids. This combination of techniques can help unravel the mechanisms of action at both micro and nanoscales and design more efficient antimicrobial compounds.
Article
Biophysics
Autumn A. Anthony, Osman Sahin, Murat Kaya Yapici, Daniel Rogers, Aurelia R. Honerkamp-Smith
Summary: This study systematically measured the coefficient of interleaflet friction in supported lipid membranes using a microfluidic technique. The results showed that this technique provides reproducible measurements of the friction coefficient and is sensitive enough to detect differences in friction between different lipid membranes. Adding cholesterol significantly increased interleaflet friction. Additionally, fluid shear stress was found to reversibly induce the gel phase in supported lipid bilayers close to the gel-transition temperature.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Eduart Gutierrez-Pineda, Patrizia Andreozzi, Eleftheria Diamanti, Ramiro Anguiano, Ronald F. Ziolo, Sergio E. Moya, Maria Jose Rodriguez-Presa, Claudio A. Gervasi
Summary: Supported Lipid Bilayers (SLBs) on Polyelectrolyte Multilayers (PEMs) have large potential as models for developing sensor devices. SLBs can be designed with receptors and channels to create a sensing interface for ions and biomarkers. The fabrication of valinomycin-doped SLBs on PEMs as a model system for biophysical studies and for selective ion sensing is studied.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jeremiah Traeger, Dehong Hu, Mengran Yang, Gary Stacey, Galya Orr
Summary: Plant cell signaling relies on the cellular organization of receptor-like kinases (RLKs) within membrane nanodomains for efficient and specific signaling. Quantitative analysis of spatial organizations of RLKs can provide new understanding of plant responses to environmental stress.
Editorial Material
Chemistry, Multidisciplinary
Anke Krueger, Nianjun Yang, Robert J. Hamers
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Geochemistry & Geophysics
Jocelyn A. Richardson, Christopher R. Anderton, Arunima Bhattacharjee
Summary: Plants need potassium for vital biological processes, but most soils lack sufficient potassium. This study investigates how the fungus Fusarium sp. DS 682 breaks down potassium-rich soil minerals using synthetic soil habitats. The distribution of secondary clays formed by the fungus differs based on the proximity to a carbon source.
Article
Chemistry, Multidisciplinary
Sophia M. McClain, Moses H. Milchberg, Chad M. Rienstra, Catherine J. Murphy
Summary: The interaction between alpha-synuclein and synaptic vesicle mimics depends on the malleability and rigidity of the mimics, with higher binding affinities observed for rigid mimics. The lipid composition of the mimics also affects the interaction. The study provides insights into the behavior of alpha-synuclein and its relation to membrane properties.
Article
Chemistry, Analytical
Gregory W. Vandergrift, Kevin J. Zemaitis, Dusan Velickovic, Jessica K. Lukowski, Ljiljana Pasa-Tolic, Christopher R. Anderton, William Kew
Summary: We assessed the complementarity and complexity of data detected in mammalian lipidome mass spectrometry imaging (MSI) using MALDI and nano-DESI. Our results showed that while MALDI had greater molecular coverage and dynamic range capabilities, nano-DESI provided superior mass error. Both modes demonstrated comparable degrees of lipidome complexity. We also evaluated the need for ultrahigh mass resolving power for certain mass splits in advanced instrumentation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Kevin J. Zemaitis, Vivian S. Lin, Amir H. Ahkami, Tanya E. Winkler, Christopher R. Anderton, Dusan Velickovic
Summary: In order to better understand the overall metabolome of a species, researchers have developed a new workflow using chemical derivatization method at a cellular scale, which can accurately identify dozens of elusive phytocompounds. This method expands the application space of spatial metabolomics and can identify more metabolites compared to traditional methods. This work paves the way for spatial hormonomics in plant samples, enhancing the detection of carboxylates, aldehydes, and other carbonyls.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Sarah M. Yannarell, Eric S. Beaudoin, Hunter S. Talley, Alexi A. Schoenborn, Galya Orr, Christopher R. Anderton, William B. Chrisler, Elizabeth A. Shank
Summary: This study investigated the spatial organization and gene expression relationships for important genes in B. subtilis biofilms, expanding the description of cell populations. The results revealed that only a few subpopulations of cells have specific gene expression, while others exhibit simultaneous expression of multiple genes. These findings provide a framework for further studying and predicting the roles of diverse cellular phenotypes in B. subtilis biofilms.
Article
Chemistry, Multidisciplinary
Deepti Sharan, Daniel Wolfson, Curtis M. Green, Paul Lemke, Alessandra G. Gavin, Robert J. Hamers, Z. Vivian Feng, Erin E. Carlson
Summary: The use of complex metal oxide nanoparticles has increased significantly in recent years, particularly in electric vehicle batteries. However, our understanding of their impact on environmental organisms has not kept pace with their usage. This study examines the effects of chronic exposure to nanoscale lithiated nickel manganese cobalt oxide (NMC) on the bacterium Shewanella oneidensis. The results show that chronic exposure to NMC leads to genetic-level variation in the bacteria, including the production of reactive oxygen species (ROS) and DNA damage.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Matthew T. Gole, Mohan T. Dronadula, Narayana R. Aluru, Catherine J. Murphy
Summary: Understanding the adsorption behavior of proteins on rough and wrinkled surfaces is crucial for biosensor and flexible biomedical device applications. This study investigates the nanoscale adsorption behavior of immunoglobulin M (IgM) and immunoglobulin G (IgG) on wrinkled and crumpled surfaces using atomic force microscopy (AFM). The results show that the presence of negative curvature on the wrinkled surface reduces protein surface coverage, mainly due to geometric hindrance and reduced binding energy, while smaller IgG molecules are not affected by this degree of curvature.
NANOSCALE ADVANCES
(2023)
Article
Biophysics
Michael J. Taylor, Hoshin Kim, William Kew, Amity Andersen, Arunima Bhattacharjee, Mark H. Engelhard, Christopher R. Anderton
Summary: In beam-based ionization methods, the substrate plays a crucial role in the desorption mechanism of molecules from surfaces. Atomistic simulations combined with density functional theory calculations were used to study the desorption of a sphingolipid from a model surface. The results provide insights into the effects of biointerfacial lipid ordering on SIMS imaging.
Article
Chemistry, Multidisciplinary
Maegen Kincanon, Catherine J. Murphy
Summary: The self-assembly of colloidal nanocrystals is of great interest for creating hierarchical nanomaterials with advanced function. Gold nanocrystals can enhance local electric fields and provide plasmonic fields for surface-enhanced spectroscopies. A selective biotinylation method was developed for gold nanorods, allowing for self-assembly upon addition of streptavidin. The self-assembly was analyzed for different sized nanorods, showing high yield for larger nanorods and low yield for smaller nanorods.
Article
Chemistry, Multidisciplinary
Shreyasi Sengupta, Swapnil B. Ambade, Tana L. O'Keefe, Falak Tawakalna, Jenny K. Hedlund Orbeck, Robert J. Hamers, Z. Vivian Feng, Christy L. Haynes, Zeev Rosenzweig
Summary: This study investigates the environmental fate of InSe nanosheets and the impact of EGCG-coated nanosheets on bacterial growth.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Thomas R. Kuech, Nasim Ganji, Caroline Anastasia, Marco D. Torelli, Eric S. Melby, Arielle C. Mensch, Emily R. Caudill, Ralf Zimmermann, Robert J. Hamers, Joel A. Pedersen
Summary: The interactions between cationic polymer-wrapped diamond nanoparticles (PAH-DNPs) and lipid bilayers are influenced by the phospholipid composition and surface charge. Electrostatic attractions and repulsions play key roles in the initial attachment and final extent of nanoparticle adhesion. The formation of a lipid corona on PAH-DNPs reduces the effective charge density and affects the adhesion extent. The inclusion of charged phospholipids decreases the attachment efficiency and extent by reducing the electrostatic attraction between PAH-DNPs and the bilayers.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Christian A. Reardon-Lochbaum, Ravithree D. Senanayake, Rocio Amaro Marquez, Kha Trinh, Khoi Nguyen L. Hoang, Tobias Rangel Guillen, Catherine J. Murphy, Robert J. Hamers, Joel A. Pedersen, Rigoberto Hernandez
Summary: Supported lipid bilayers are commonly used as model systems for studying interactions between biological membranes and proteins or nanoparticles. The choice of substrate material, such as gold or SiO2, influences the interactions and adsorption behavior of anionic nanoparticles and cytochrome c to the bilayers. The underlying substrate material plays a role in determining the extent of interaction between nanoscale analytes and supported lipid bilayers.
ENVIRONMENTAL SCIENCE-NANO
(2023)