Article
Chemistry, Multidisciplinary
Rikhia Ghosh, Vahid Satarifard, Andrea Grafmueller, Reinhard Lipowsky
Summary: In this study, budding and fission processes of lipid nanovesicles with a size below 50 nm were reported using coarse-grained molecular dynamics simulations. The processes are influenced by solute concentration and solvent conditions, with budding being reversible and unexpected morphological transformations observed under poor solvent conditions. This reveals a nanoscale mechanism for the budding and fission of nanovesicles, arising from the interplay between membrane elasticity and solute-mediated membrane adhesion.
Article
Biochemistry & Molecular Biology
Guanghui Yang, Rui Zhou, Xuefei Guo, Chuangye Yan, Jianlin Lei, Yigong Shi
Summary: The development of GSIs and GSMs for Alzheimer's disease and cancer treatment presents a promising therapeutic opportunity. Cryo-electron microscopy structures reveal that these inhibitors and modulators target specific regions of gamma-secretase, interfering with substrate recruitment. Shared themes and variations in inhibitor and modulator recognition may guide the development of next-generation substrate-selective inhibitors.
Article
Biochemistry & Molecular Biology
Ruyue He, Furong Liu, Hui Wang, Shuai Huang, Kai Xu, Conggang Zhang, Yinghui Liu, Haijia Yu
Summary: In this study, it was discovered that ORP9 and ORP10 form a binary complex through intermolecular coiled-coil domain interaction. The PH domains of ORP9 and ORP10 specifically interact with PI4P and play a critical role in TGN targeting and regulating PI4P levels. ORP9 and ORP10 complex accelerate the transfer of PI4P between membranes and their PH domains participate in membrane tethering, while the ORD domains are required for lipid transport. Depletion of ORP9 and ORP10 leads to increased vesicle transport to the plasma membrane.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biophysics
Lihua Jiang, Qiong Wang, Jincan Lei, Ke Tao, Jing Huang, Shixian Zhao, Ning Hu, Jun Yang
Summary: The mechanisms for the failure of GLV electroformation in salt solutions are mainly two aspects: sodium chloride accelerates the assembly of membranes and reduces the permeability to aqueous solution; Sodium chloride increases the medium conductivity and decreases the electrohydrodynamic force.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Jeel Raval, Ales Iglic, Wojciech Gozdz
Summary: The study investigates the adhesion of lipid vesicles to a rigid flat surface and examines the influence of membrane curvature, adhesion strength, and reduced volume on stability and shape transformations. It determines the minimum adhesion strength needed to stabilize adhered vesicle shapes and discusses how changes in adhesion strength can induce vesicle budding. The importance of free vesicle shape for adhesion susceptibility is also highlighted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Inkyu Lee, Yoonjeong Choi, Dong-U Shin, Minjeong Kwon, Seohyun Kim, Hanul Jung, Gi-Hoon Nam, Minsu Kwon
Summary: Extracellular vesicles (EVs) are naturally released nanovesicles that have a lipid bilayer-bound form. Small EVs (sEVs) with a size of 200 nm have revealed various biological functions and potential as future drug modalities. However, there are limitations and obstacles in the clinical translation of sEV therapeutics. This review summarizes different types of sEV therapeutics, future improvements, and current strategies in large-scale production.
Article
Multidisciplinary Sciences
Mithu Baidya, Madhu Chaturvedi, Hemlata Dwivedi-Agnihotri, Ashutosh Ranjan, Dominic Devost, Yoon Namkung, Tomasz Maciej Stepniewski, Shubhi Pandey, Minakshi Baruah, Bhanupriya Panigrahi, Parishmita Sarma, Manish K. Yadav, Jagannath Maharana, Ramanuj Banerjee, Kouki Kawakami, Asuka Inoue, Jana Selent, Stephane A. Laporte, Terence E. Hebert, Arun K. Shukla
Summary: This study reveals that synthetic antibodies can be used to modulate GPCR trafficking and signaling in live cells.
NATURE COMMUNICATIONS
(2022)
Article
Polymer Science
Che-Lun Chin, Lu-Jan Huang, Zheng-Xian Lu, Wei-Chun Weng, Ling Chao
Summary: This study demonstrates that the water absorption ability of dried PAAm hydrogel can facilitate the formation of hydrogel-SLBs. The high quality of the hydrogel surface is crucial for SLB formation, and minimizing oxygen exposure during drying can prevent the formation of an oxidized crust.
Article
Biochemistry & Molecular Biology
Wei Dong, Xubo Zhang, Yue Kong, Zhenwen Zhao, Ali Mahmoud, Lixian Wu, Bernard Moussian, Jianzhen Zhang
Summary: The study demonstrates that the midgut-specific CYP311A1 in fruit flies plays a crucial role in lipid distribution by shaping microvilli in a PE-dependent manner.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Immunology
Nathan R. Allen, Aspen R. Taylor-Mew, Toby J. Wilkinson, Sharon Huws, Helen Phillips, Russell M. Morphew, Peter M. Brophy
Summary: This study investigated the extracellular vesicles released by adult Calicophoron daubneyi, revealing their important role in establishment and maintenance of infection, as well as their potential to control rumen fluke infection and improve rumen efficiency by modulating microbial populations within the rumen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Hideki Kanda, Tsubasa Katsube, Wahyudiono, Motonobu Goto
Summary: A new method for preparing liposomes using liquefied DME was investigated in this study, which can avoid the toxicity and residue issues of conventional solvents, and is environmentally friendly and cost-effective.
Article
Chemistry, Analytical
Ruyan Niu, Xiaohui Chen, Zixin Sun, Liu Wang, Zining Wang, Chong Zhang, Dan Ding, Jichun Yang, Yongzhong Wang, Yang Luo
Summary: Cancer-derived small extracellular vesicles (csEVs) are important indicators for the presence and progression of malignancies, but discriminating csEVs from normal sEVs and detecting them at low abundance in early-stage cancer is challenging. In this study, the researchers developed a TESTER strategy based on MNAzyme-controlled recognition to selectively identify csEVs from complex clinical samples. The TESTER approach achieved highly sensitive and selective quantification of csEVs with a detection threshold of 72 particles/μL, and the researchers also constructed a new platform for bimolecular simultaneous recognition, providing insights for future development of bimolecular-activated detection switches.
ANALYTICA CHIMICA ACTA
(2023)
Article
Engineering, Electrical & Electronic
Qiuyuan Wang, Yi Zeng, Kai Yuan, Qingqi Zeng, Pingfan Gu, Xiaolong Xu, Hanwen Wang, Zheng Han, Kentaro Nomura, Wenhong Wang, Enke Liu, Yanglong Hou, Yu Ye
Summary: The efficiency of spintronic devices can be improved by generating higher effective magnetic fields with lower working currents. Spin-transfer torques can drive magnetic domain wall motion in a device composed of a single material. Here, we report magnetism modulation in Co3Sn2S2-a magnetic Weyl semimetal-via spin-transfer-torque-driven domain wall motion. The threshold current density for driving domain wall motion is less than 5.1 x 10(5) A cm(-2) at zero external field and less than 1.5 x 10(5) A cm(-2) at a moderate external field (0.2 kOe). The spin-transfer-torque effective field can reach as high as 2.4-5.6 kOe MA(-1) cm(2) at 150 K.
NATURE ELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Kathleen W. Swana, Terri A. Camesano, Ramanathan Nagarajan
Summary: Supported lipid bilayers (SLBs) on quartz crystals are versatile model systems for studying cell membrane behavior. This study successfully formed entirely anionic SLBs by treating the quartz crystal, which provides valuable model systems for studying interactions with Gram-positive bacterial membranes.
Article
Multidisciplinary Sciences
Maurice Michel, Carlos Benitez-Buelga, Patricia A. Calvo, Bishoy M. F. Hanna, Oliver Mortusewicz, Geoffrey Masuyer, Jonathan Davies, Olov Wallner, Kumar Sanjiv, Julian J. Albers, Sergio Castaneda-Zegarra, Ann-Sofie Jemth, Torkild Visnes, Ana Sastre-Perona, Akhilesh N. Danda, Evert J. Homan, Karthick Marimuthu, Zhao Zhenjun, Celestine N. Chi, Antonio Sarno, Elisee Wiita, Catharina von Nicolai, Anna J. Komor, Varshni Rajagopal, Sarah Muller, Emily C. Hank, Marek Varga, Emma R. Scaletti, Monica Pandey, Stella Karsten, Hanne Haslene-Hox, Simon Loevenich, Petra Marttila, Azita Rasti, Kirill Mamonov, Florian Ortis, Fritz Schoemberg, Olga Loseva, Josephine Stewart, Nicholas D'Arcy-Evans, Tobias Koolmeister, Martin Henriksson, Dana Michel, Ana de Ory, Lucia Acero, Oriol Calvete, Martin Scobie, Christian Hertweck, Ivan Vilotijevic, Christina Kalderen, Ana Osorio, Rosario Perona, Alexandra Stolz, Pal Stenmark, Ulrika Warpman Berglund, Miguel de Vega, Thomas Helleday
Summary: We describe a small molecule that interacts with OGG1, increases enzyme activity and generates a new lyase function. This molecule controls catalytic activity and alters the repair process of oxidative DNA damage. The findings may have therapeutic applications in various diseases and aging.