Article
Multidisciplinary Sciences
Xiang Feng, Gerrit J. Schut, Dominik K. Haja, Michael W. W. Adams, Huilin Li
Summary: Electron bifurcation allows energetically unfavorable biochemical reactions to occur. This study reports a new bifurcating flavoenzyme structure that couples hydrogen oxidation with NAD reduction through electron transfer. By elucidating its structure and function, we gain a deeper understanding of the electron transfer pathways in hydrogenases.
Article
Chemistry, Multidisciplinary
Armel F. T. Waffo, Christian Lorent, Sagie Katz, Janna Schoknecht, Oliver Lenz, Ingo Zebger, Giorgio Caserta
Summary: This study used cryogenic infrared and electron paramagnetic resonance spectroscopy to decipher the structural basis of the Ni-a-L intermediates in the regulatory [NiFe]-hydrogenase from Cupriavidus necator, revealing the importance of the protein scaffold in fine-tuning proton and electron dynamics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Microbiology
Qin Fan, Giorgio Caserta, Christian Lorent, Ingo Zebger, Peter Neubauer, Oliver Lenz, Matthias Gimpel
Summary: In this study, the production process of heterologous hydrogenase in E. coli was significantly improved to obtain catalytically active regulatory hydrogenase (RH). Important factors such as O-2 content, metal availability, production conditions, and co-expression of RH-specific maturase genes were optimized. The maturation of RH was successfully achieved during aerobic cultivation of E. coli by co-production of seven hydrogenase-specific maturases and a nickel permease. The improved production conditions resulted in a high yield of catalytically active RH and an increased space-time yield in E. coli compared to the native host C. necator. This strategy has important implications for the production of catalytically active [NiFe]-hydrogenases in biotechnologically relevant quantities.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Rhiannon M. Evans, Natalie Krahn, Bonnie J. Murphy, Harrison Lee, Fraser A. Armstrong, Dieter Soll
Summary: The study investigates the impact of substituting cysteine with selenocysteine on the function of [NiFe]-hydrogenases, revealing that while the activity of the enzymes is reduced after substitution, variants with selenocysteine show significant differences in O-2 tolerance and electrocatalytic properties.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Qin Fan, Saskia Waldburger, Peter Neubauer, Sebastian L. Riedel, Matthias Gimpel
Summary: This study successfully increased the yield and quality of NiFe-hydrogenase by optimizing culture conditions and designing a high-density fed-batch cultivation process. It provides a good opportunity to economically supply complex metalloproteins at high concentrations to meet the demand in basic and applied studies.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Multidisciplinary
Jinmei Yang, Yuxian Lu, Lei Jin, Chunxiao Zhao, Yuang Chen, Yang Xu, Fanfan Chen, Jiandong Feng
Summary: This study optically visualized proton transport pathways through nanopores and hydrophilic interfaces using fluorescence signals, revealing an increase in fluorescence intensity at negative voltage due to lateral transport. With the temporal resolution of optical imaging, the technique enabled a comprehensive analysis of proton transport dynamics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Giorgio Caserta, Vladimir Pelmenschikov, Christian Lorent, Armel F. Tadjoung Waffo, Sagie Katz, Lars Lauterbach, Janna Schoknecht, Hongxin Wang, Yoshitaka Yoda, Kenji Tamasaku, Martin Kaupp, Peter Hildebrandt, Oliver Lenz, Stephen P. Cramer, Ingo Zebger
Summary: Understanding the relationship between [NiFe]-hydrogenases and their resting states is crucial for elucidating their catalytic mechanism and enzymatic activity.
Article
Microbiology
Pier Francesco Di Leonardo, Giacomo Antonicelli, Valeria Agostino, Angela Re
Summary: Understanding the organizational and functional properties of hydrogen metabolism is crucial for creating a framework to support a hydrogen-fueled low-carbon economy. This study investigated the genomes of several industrially relevant acetogens and identified their hydrogenases' repertoire. The findings provide valuable knowledge on the functional specificity and potential applications of hydrogenases in biotechnological processes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
Christian Lorent, Vladimir Pelmenschikov, Stefan Frielingsdorf, Janna Schoknecht, Giorgio Caserta, Yoshitaka Yoda, Hongxin Wang, Kenji Tamasaku, Oliver Lenz, Stephen P. Cramer, Marius Horch, Lars Lauterbach, Ingo Zebger
Summary: A new experimental setup was developed to study metalloenzymes in detail, allowing the controlled preparation and characterization of catalytic intermediates using various spectroscopic techniques. By investigating two O-2-tolerant [NiFe]-hydrogenases as model systems, detailed insights into the catalytic mechanisms and vibrational fingerprints were revealed. Additionally, the study highlighted the importance of complementing X-ray crystallographic data with spectroscopic analyses.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Ravi Kumar, Matthias Stein
Summary: Recently, it was discovered that the oxygen tolerance of the [NiFe]-hydrogenase from H. thermoluteolus stems from an unusual coordination sphere of the active site nickel atom. In the oxidized state, a terminal cysteine residue is displaced by a bidentate coordinating nearby glutamate, resulting in its occupation of a third bridging position.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Giorgio Caserta, Sven Hartmann, Casey Van Stappen, Chara Karafoulidi-Retsou, Christian Lorent, Stefan Yelin, Matthias Keck, Janna Schoknecht, Ilya Sergueev, Yoshitaka Yoda, Peter Hildebrandt, Christian Limberg, Serena DeBeer, Ingo Zebger, Stefan Frielingsdorf, Oliver Lenz
Summary: In this study, the maturation process of [NiFe]-hydrogenase was investigated using biochemical analysis and various spectroscopic techniques, providing detailed insights into its complex maturation mechanism.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Physical
Yuanming Wang, Zepeng Kang, Lingling Zhang, Zhiguang Zhu
Summary: This study investigates the interactions between PfSHI and carbon nanotubes, revealing that electrostatic and hydrophobic interactions play a crucial role in the interaction between PfSHI and electrodes. Based on these findings, a high-performance biofuel cell was constructed, demonstrating an improved power output.
Article
Materials Science, Biomaterials
Qiuyao Jiang, Tianpei Li, Jing Yang, Catherine M. Aitchison, Jiafeng Huang, Yu Chen, Fang Huang, Qiang Wang, Andrew I. Cooper, Lu-Ning Liu
Summary: Researchers engineered the protein organelle in bacteria cells to encapsulate hydrogenases, resulting in improved hydrogen production and catalytic efficiency. This study provides a framework for developing new bio-inspired electrocatalysts for sustainable fuel and chemical production.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biotechnology & Applied Microbiology
Sven Lahme, Jaspreet Mand, John Longwell, Ramsey Smith, Dennis Enning
Summary: Methanogenic archaea play a role in MIC in oil field pipelines, with a newly discovered mechanism involving a (NiFe) hydrogenase. A specific qPCR assay was developed to detect the gene associated with this mechanism, offering a step toward understanding and managing MIC. The proposed MIC biomarker was detected in multiple oil fields, indicating widespread application potential.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Chemistry, Analytical
Ibukun Oluwaseun Shotonwa, Onome Ejeromedoghene, Adedoyin Oluseyi Adesoji, Yakubu Adekunle Alli, Caroline Akinremi, Sheriff Adewuyi
Summary: Iron-sulphur cofactors are important in biological redox reactions and mimicking them for hydrogen production has attracted global attention. Coordinatively saturated metal complex systems show remarkable activity for proton reduction compared to their unsaturated counterparts. The mechanisms of hydride formation in these systems can be investigated using experimental, spectroscopic, and computational techniques. This review highlights the connection between the electrochemistry and electrocatalysis of Fe-Fe-hydrogenase mimics and proposes future catalyst designs based on structure-catalytic activity relationships.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
R. Hunter Wilson, Serban Zamfir, Isaiah Sumner
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2017)
Article
Chemistry, Physical
Rebekah M. Soliday, Hayley Bunn, Isaiah Sumner, Paul L. Raston
JOURNAL OF PHYSICAL CHEMISTRY A
(2019)
Meeting Abstract
Biophysics
Katherine Elliott, N. Cole Seward, Heath Hampton, Isaiah Sumner
BIOPHYSICAL JOURNAL
(2019)
Meeting Abstract
Biophysics
Isaiah Sumner, Walker M. Jones, Aaron G. Davis, R. Hunter Wilson, Katherine L. Elliott
BIOPHYSICAL JOURNAL
(2019)
Letter
Chemistry, Physical
Killian Hull, Rebekah M. Soliday, Isaiah Sumner, Paul L. Raston
JOURNAL OF CHEMICAL PHYSICS
(2019)
Article
Chemistry, Multidisciplinary
Walker M. Jones, Aaron G. Davis, R. Hunter Wilson, Katherine L. Elliott, Isaiah Sumner
JOURNAL OF COMPUTATIONAL CHEMISTRY
(2019)
Article
Chemistry, Physical
Isaiah Sumner, Hannah Anthony
INTERNATIONAL JOURNAL OF QUANTUM CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Jay-Anne K. Johnson, Isaiah Sumner
Summary: Ubiquitination is a post-translational modification process that signals for various regulatory pathways, including protein degradation, by covalently binding small protein ubiquitin (Ub) to a lysine on the target protein. This study investigates the hypothesis that the RING E3 enhances ubiquitination efficiency by placing the E2 similar to Ub conjugate in a closed position. Molecular dynamics simulations show that the closed configuration strains the thioester bond and increases the populations of key hydrogen bonds, consistent with the experimental rate enhancement caused by the RING E3.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Meeting Abstract
Chemistry, Multidisciplinary
Rebekah Soliday, Isaiah Sumner, Paul Raston
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Meeting Abstract
Chemistry, Multidisciplinary
Ryan Wilson, Isaiah Sumner
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Meeting Abstract
Chemistry, Multidisciplinary
Walker Jones, Aaron Davis, R. Wilson, Serban Zamfir, Isaiah Sumner
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Astronomy & Astrophysics
Hayley Bunn, Rebekah M. Soliday, Isaiah Sumner, Paul L. Raston
ASTROPHYSICAL JOURNAL
(2017)
Meeting Abstract
Biophysics
Walker M. Jones, Aaron Davis, Isaiah Sumner, Serban Zamfir
BIOPHYSICAL JOURNAL
(2017)
Meeting Abstract
Biophysics
Isaiah Sumner, R. Hunter Wilson, Walker M. Jones, Aaron G. Davis, Serban Zamfir
BIOPHYSICAL JOURNAL
(2017)
Meeting Abstract
Biophysics
R. Hunter Wilson, Isaiah Sumner
BIOPHYSICAL JOURNAL
(2017)