Article
Agriculture, Multidisciplinary
Jyoti Jaiswal, Amit Kumar Srivastav, Pradeep Kumar Rajput, Umesh C. S. Yadav, Umesh Kumar
Summary: This study aimed to improve the solubility and stability of cordycepin by encapsulating it in bovine serum albumin: beta-cyclodextrin nanoparticles. The synthesized nanoparticles showed sustained release of cordycepin and improved stability. In vitro studies also demonstrated greater cytotoxicity of the synthesized nanoparticles compared to free cordycepin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Physical
Mohd Sajid Ali, Jayaraman Muthukumaran, Monika Jain, Teresa Santos-Silva, Hamad A. Al-Lohedan, Noura Saad Al-Shuail
Summary: The study investigated the binding of the cephalosporin-class drug cefoperazone with bovine serum albumin using spectroscopic techniques and in silico methods. The importance of correcting for the inner filter effect in this type of study was emphasized, and the binding mechanism was found to involve hydrogen bonding and hydrophobic forces with almost 1:1 static binding between BSA and CFP. Molecular docking and molecular dynamics simulation suggested the presence of a putative binding site between BSA domains 1 and 3, which could be a target for future research related to BSA-drug binding.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Physical
Monika Kumari, Pratibha Kumari, Hemant K. Kashyap
Summary: The use of conventional solvents in biotransformation processes has raised concerns about environmental impact and challenges. Deep eutectic solvents (DESs) have emerged as promising alternatives with improved biocompatibility and potential applications in protein engineering and crystallization. This study investigates the impact of reline DES in its pure and hydrated forms on the structural stability and conformation of bovine serum albumin (BSA) protein using molecular dynamics simulations. The results reveal that reline induces structural perturbations and disrupts the secondary structure of BSA in a concentration-dependent manner. Despite the expansion, BSA structure in pure reline remains close to the native structure and can partially recover after re-equilibration.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Physical
Tatsuya Tsukahara, Kaito Sasaki, Rio Kita, Naoki Shinyashiki
Summary: In this study, dielectric relaxations of ice in low-concentration protein-water mixtures were investigated. The relaxation time of ice changes twice at specific temperatures in these mixtures, and below 200 K, the relaxation of ice separates into multiple relaxations. The previously reported relaxation of uncrystallized water was confirmed to be one of the multiple relaxations of ice.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Spectroscopy
Md. Zahirul Kabir, Hafsa Tayyab, Cem Erkmen, Sevinc Kurbanoglu, Saharuddin B. Mohamad, Bengi Uslu
Summary: The interactive association between the antifungal drug climbazole (CBZ) and the carrier protein bovine serum albumin (BSA) was investigated using fluorescence, absorption spectroscopy, and in silico techniques. The results confirmed the formation of a complex between CBZ and BSA. Hydrophobic forces, van der Waals forces, and hydrogen bonds were found to play key roles in stabilizing the complex. The binding of CBZ to BSA occurred near Sudlow's sites I and II, with a greater preference for site II.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Physical
Hurija Dzudzevic Cancar, Matic Belak Vivod, Vojko Vlachy, Miha Luksic
Summary: The stability of bovine serum albumin (BSA) solutions against phase separation caused by cooling the system is studied under the combined influence of added poly(ethylene glycol) (PEG) and alkali halide salts in water as solvent. The phase stability of the system depends on the concentration of the added PEG and its molecular mass, the concentration of the low molecular mass electrolyte and its nature, as also on the pH of the solution.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Applied
Mingmin Zhang, Robin A. Hutchinson
Summary: Three process variations were developed to synthesize dextran-bovine serum albumin (BSA) model conjugate through the Maillard reaction and a Schiff base-Amadori solid phase rearrangement mechanism. The purified conjugate solution was further heated to form nanogels with a hydrodynamic diameter of 195-400 nm.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Mohd Sajid Ali, Md Tabish Rehman, Hamad A. Al-Lohedan, Mohamed Fahad AlAjmi
Summary: The study showed weak binding between cuminol and BSA, mainly through a static quenching mechanism. The binding was supported by hydrophobic forces with a minor contribution from hydrogen bonding. While the secondary structure of BSA remained relatively unchanged at low cuminol concentrations, partial unfolding occurred with higher cuminol concentrations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Chemistry, Physical
Shirin Mollazadeh, Saeed Babaei, Mehdi Ostadhassan, Rezvan Yazdian-Robati
Summary: This study investigates the concentration-dependent assembly behavior of BSA molecules in the presence of protonated DOXs using molecular dynamics simulations. The results show that higher concentration leads to the formation of larger nanoparticles with a change in surface charge from negative to positive, and increased protein and loaded DOXs content. Additionally, the study highlights the significance of acidic amino acids and DOX for this assembly process. It suggests that increasing the molecular concentration can improve the drug delivery efficiency of BSA.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Physical
Aurica Precupas, Romica Sandu, Alexandru Vincentiu Florian Neculae, Andreea Neacsu, Vlad Tudor Popa
Summary: Morin was found to have two binding sites with bovine serum albumin and could increase the thermal stability of the protein. Molecular docking and dynamic simulations supported the binding receptors of Morin with BSA. The dual action of Morin was shown to stabilize the protein structure and promote aggregation.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Physical
Aleksandra Tesmar, Malgorzata M. Kogut, Krzysztof Zamojc, Ola Grabowska, Katarzyna Chmur, Sergey A. Samsonov, Joanna Makowska, Dariusz Wyrzykowski, Lech Chmurzynski
Summary: The study investigates the interactions between SDS and BSA, revealing at least two structurally distinct binding sites in BSA at low molar ratios. The formation of SDS-BSA complexes is driven by enthalpy, with the first binding site playing a crucial role in thermally stabilizing the helical conformation of BSA.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Review
Biochemistry & Molecular Biology
Xinhao Xu, Jinyu Hu, Huaqian Xue, Yingying Hu, Ya-nan Liu, Guanyang Lin, Liangle Liu, Ren-ai Xu
Summary: This paper reviews the applications of human and bovine serum albumin in biomedical engineering, including their structural differences, extraction methods, characteristics, functions, applications, and future research directions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Mykola Seredych, Kathleen Maleski, Tyler S. S. Mathis, Yury Gogotsi
Summary: This study reports on the formation of stable colloidal solutions of MXene nanosheets using a dispersing and electrostatic exfoliation method in the presence of albumin. The albumin-coated MXenes showed good stability and can find applications in various fields due to the significant role of protein adsorption onto nanomaterial surfaces.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Food Science & Technology
Lei Xu, Hu Hou, Brian Farkas, Kevin M. Keener, Allen L. Garner, Bernard Tao
Summary: This study investigated the effects of high voltage atmospheric cold plasma on bovine serum albumin, leading to structural alterations, protein precipitation, and peptide bond cleavage. The findings suggest that interactions between HVACP-induced reactive species and proteins can introduce significant changes to protein structure and composition in aqueous conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohd Sajid Ali, Jayaraman Muthukumaran, Monika Jain, Hamad A. Al-Lohedan, M. Abul Farah, Osama Ibrahim Alsowilem
Summary: This study investigates the binding mechanism of the anticancer drug gemcitabine with the plasma protein BSA using various experimental and computational methods. The formation of a complex between gemcitabine and BSA was confirmed, with the involvement of tyrosine residues in fluorescence quenching highlighted. The primary binding site of gemcitabine in BSA was identified as drug binding site 2 (DS II), with hydrophobic forces and hydrogen bonding being the dominant intermolecular forces.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Thermodynamics
Jun Fujimura, Minato Sugisaki, Eri Nishiyama, Masanobu Takatsuka, Kaito Sasaki, Naoki Shinyashiki, Itaru Tsukushi
THERMOCHIMICA ACTA
(2019)
Article
Thermodynamics
I. Dueramae, M. Yoneyama, N. Shinyashiki, S. Yagihara, R. Kita
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2019)
Article
Dermatology
Yuko Maruyama, Hayato Kamata, Seiei Watanabe, Rio Kita, Naoki Shinyashiki, Shin Yagihara
SKIN RESEARCH AND TECHNOLOGY
(2020)
Article
Chemistry, Physical
Mitsuki Fujii, Kaito Sasaki, Yurika Matsui, Shiori Inoue, Rio Kita, Naoki Shinyashiki, Shin Yagihara
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)
Article
Physics, Multidisciplinary
Kaito Sasaki, Kenta Bandai, Masanobu Takatsuka, Mitsuki Fujii, Minato Takagi, Rio Kita, Shin Yagihara, Hiroshi Kimura, Naoki Shinyashiki
FRONTIERS IN PHYSICS
(2020)
Article
Chemistry, Physical
Kaito Sasaki, Masanobu Takatsuka, Naoki Shinyashiki, Kia L. Ngai
Summary: Linking the dynamics of mixtures of water with hydrophilic molecules and polymers and hydrated proteins to glass-forming substances is a common practice in research community. The dynamics of poly(vinyl pyrrolidone)-water mixtures resemble that of highly asymmetric mixtures, both governed by the same physics. This study expands the understanding of the physics behind the dynamics in polymer-water mixtures, with potential implications for hydrated proteins.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Multidisciplinary Sciences
Risa Otagiri, Hideki Kawai, Masanobu Takatsuka, Naoki Shinyashiki, Akira Ito, Ryosuke Ikeguchi, Tomoki Aoyama
Summary: Electrical stimulation is a candidate for regeneration of damaged peripheral nerves, with tissue-specific electrical properties and differences in electrical reactions between injured and non-injured nerves potentially influenced by inflammation and edema. The suitable frequency range for interfacial polarization is critical for electrical stimulation of injured peripheral nerves.
Article
Polymer Science
Balachandar Vijayakumar, Masanobu Takatsuka, Rio Kita, Naoki Shinyashiki, Shin Yagihara, Sampathkumar Rathinasabapathy
Summary: In this study, non-ionic poly(N-isopropylacrylamide) (PNIPAM) microgel particles were synthesized using free-radical precipitation polymerization. The properties and behavior of these particles were investigated through dynamic light scattering and dielectric experiments.
Article
Chemistry, Physical
Marcin Pastorczak, Michal Nejbauer, Naoki Shinyashiki, Masanobu Takatsuka, Gonzalo Angulo, Yuriy Stepanenko, Czeslaw Radzewicz
Summary: The study observed for the first time the sub-nanosecond stage of the coil-to-globule transition of a polymer in water, which was made possible by a novel experimental approach. The increase in light scattering intensity around 300-400 ps after a temperature jump above the LCST suggests the rapid formation of local nuclei along the polymer chain in the observed stage of the transition.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Polymer Science
Isala Dueramae, Taisei Ishiyama, Ayumi Torigaki, Shinya Nakano, Kaito Sasaki, Rio Kita, Naoki Shinyashiki, Shin Yagihara, Yukiteru Katsumoto, Masaru Yoneyama
Summary: The dielectric relaxation of poly-(N-isopropylacrylamide) (PNiPAM) in 1,4-dioxane as a low-polarity solvent was investigated under various conditions. The study found that the relaxation is composed of two components: rotational motion of side groups at around 100 MHz and micro-Brownian motion of PNiPAM chain at around 1 MHz. These processes can be described by two Cole-Cole equations. The relaxation times of these processes showed temperature dependence, as evidenced by the activation energies obtained from the Arrhenius plot. Additionally, asymmetric relaxation was observed in the MHz region of PNiPAM in different solvents, suggesting a deeper understanding of PNiPAM's molecular dynamics.
Article
Polymer Science
Shin Yagihara, Seiei Watanabe, Yuta Abe, Megumi Asano, Kenta Shimizu, Hironobu Saito, Yuko Maruyama, Rio Kita, Naoki Shinyashiki, Shyamal Kumar Kundu
Summary: This paper presents a universal water structure analysis of several gels with different structures and gelation mechanisms including polymer gels, supramolecular gels composed of surfactant micelles, and cement gels. The results show that the hydrogen bond network (HBN) of water molecules in these gels undergoes changes at a molecular level, with a decrease in fractal dimension as cross-linking increases, leading to the fragmentation of collective water structures. The tau-beta diagrams analysis method proposed in this paper can be widely applied to characterize diverse gel structures and evaluate gelation processes.
Article
Polymer Science
Kenta Shimizu, Fumiya Abe, Yasuhiro Kishi, Rio Kita, Naoki Shinyashiki, Shin Yagihara
Summary: There are diverse gel materials used in various fields and their gelation mechanisms are extremely varied. In the case of hydrogels, it is challenging to understand the complex molecular mechanisms due to the interaction of water molecules through hydrogen bonding as solvents. This study used broadband dielectric spectroscopy (BDS) to elucidate the molecular mechanism of the structural formation of fibrous supramolecular gel by a low molecular weight gelator, N-oleyl lactobionamide/water mixture.
Article
Biology
Kahori Furuhata, Haruchika Masuda, Atsuko Sato, Kumiko Miyata, Naoki Shinyashiki, Rio Kita, Kotaro Imagawa, Tadashi Akamatsu, Shin Yagihara
Summary: This study examined the in vivo water structure dynamics (WSD) between physiological and pathophysiological statuses. The results showed that tumor-bearing mice exhibited aberrant WSD compared to healthy mice. Dielectric spectroscopy may provide novel pathophysiological perspectives in tumor-bearing living bodies.
Article
Chemistry, Physical
Balachandar Vijayakumar, Masanobu Takatsuka, Kaito Sasaki, Rio Kita, Naoki Shinyashiki, Shin Yagihara, Sampathkumar Rathnasabapathy
Summary: A broadband dielectric spectroscopy study was conducted to investigate the dielectric relaxation of ice in a partially crystallized poly(N-isopropylacrylamide) microgel aqueous suspension. Two relaxation processes were observed, one associated with the local chain motion of the microgel and interfacial polarization, and the other associated with ice. The temperature dependence of the ice relaxation time was found to be independent for concentrations ≥10 wt% and the influence of different polymer water mixtures on ice crystallization was studied.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Tatsuya Tsukahara, Kaito Sasaki, Rio Kita, Naoki Shinyashiki
Summary: In this study, dielectric relaxations of ice in low-concentration protein-water mixtures were investigated. The relaxation time of ice changes twice at specific temperatures in these mixtures, and below 200 K, the relaxation of ice separates into multiple relaxations. The previously reported relaxation of uncrystallized water was confirmed to be one of the multiple relaxations of ice.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)