期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 115, 期 5, 页码 1165-1174出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp107558e
关键词
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资金
- National Natural Science Foundation of China [11074747]
- Research Fund for the Doctoral Program of Higher Education of China [RFDP-20100071110006]
- Program for New Century Excellent Talents in University [NCET-08-0125]
A beta 25-35, a proteolytic fragment of the Alzheimer amyloid beta (A beta) peptide, is produced in the brains of Alzheimer's patients and retains the neurotoxicity of its full-length counterpart. The formation of pores/channels in membranes has been reported as one of the mechanisms responsible for A beta 25-35 toxicity. In addition, it has been proposed that pore/channel might be formed by the aggregation of A beta 25-35 in membranes into a beta-barrel structure. However, the structure of the beta-barrel and its perturbation on the ordering of lipid bilayer at atomic level remain elusive. In this study, we have investigated the interactions of three types of. preformed A beta 25-35 beta-barrels (labeled as barrels A, B, and C) with negatively charged palmitoyloleoylphosphatidylglycerol (POPG) lipid bilayers using all-atom molecular dynamics (MD) simulations. Each type of A beta 25-35 beta-barrel consists of eight beta-strands with positively charged side chains of lysine residues oriented toward the interior or exterior of the barrel. Barrels A, B, and C have respectively an out-of-register mixed parallel-antiparallel. (taken from our previous study), in-register mixed parallel-antiparallel, and in-register antiparallel beta-strand arrangements. Simulations have been performed by employing the initial configurations where the beta-barrels are fully or partially inserted into the bilayer. On the basis of nine independent 150 ns MD runs for the full-insertion system, we found that barrels A and C slightly affect the local ordering of lipid bilayer, while barrel B perturbs the local structure of membrane and even causes membrane leakage for water by forming nanometer-sized hydrophilic pore when lysine residues on its inner Side. Two 100 ns MD simulations on partial-insertion system show that partial insertion of A beta 25 35 beta-barrel in the bilayer results in a tendency to stay inside for barrel B. These results suggest that barrel B with Lys residues on its inner side is the most likely A beta 25-35 pore structure leading to membrane leakage. Our MD simulations provide significant insight into the atomic resolution structure of A beta 25-35 beta-sheet-rich pores and the membrane disruption mechanism induced by A beta 25-35 amyloid pores.
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