Article
Polymer Science
Xiaotong Fan, Ke Li, Siqi Liu, Tingting Wang, Yedong Ma, Zibiao Li, Chaobin He
Summary: Protein nanotubes were fabricated by coordinating imidazole-grafted horseradish peroxidase nanogels with Cu2+. The size of the nanotubes was adjustable by changing the amount of Cu2+ added, and a highly sensitive H2O2 detection system was established using these nanotubes. This work provides a facile method for constructing diverse and sophisticated functional protein nanomaterials.
Article
Environmental Sciences
Yixin Huang, Jinbin Lin, Jing Zou, Jiaxin Xu, Mengyun Wang, Huahua Cai, Baoling Yuan, Jun Ma
Summary: Introduction of ABTS as an electron shuttle in the HRP/H2O2 system enhanced the degradation efficiency of diclofenac under neutral pH conditions. The green-colored ABTS radical was identified as the main reactive species in the HRP/H2O2/ABTS system, showing no destruction and serving as an ideal electron shuttle. The reaction conditions including solution pH, HRP concentration, and H2O2 concentration impacted the formation of ABTS radical and the degradation of diclofenac in the system.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biophysics
Sara Bocanegra-Rodriguez, Carmen Molins-Legua, Pilar Campins-Falco, Fabien Giroud, Andrew J. Gross, Serge Cosnier
Summary: The non-covalent modification of carbon nanotube electrodes with pyrene derivatives is a versatile approach to enhance the electrical wiring of enzymes for biosensors and biofuel cells. The study found that pyrene-modified electrodes showed improved catalytic reduction with horseradish peroxidase (HRP), indicating great potential for biosensing and biofuel cell applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Environmental Sciences
Jing-Jing Liu, Jong-Gook Kim, Hye-Bin Kim, Shakya Abeysinghe, Ying-Wu Lin, Kitae Baek
Summary: Enzyme-based biocatalytic treatment is an effective method for degrading organic pollutants. In this study, horseradish peroxidase (HRP) was immobilized on functionalized rice straw biochar using glutaraldehyde as a crosslinker. The biochar was pretreated by electrochemical and acid treatments to enhance its immobilizing ability.
Article
Environmental Sciences
Luis Angel Zambrano-Intriago, Celia G. Amorim, Alberto N. Araujo, Dmitrij Gritsok, Joan Manuel Rodriguez-Diaz, Maria C. B. S. M. Montenegro
Summary: This work describes the development of an affordable, simple, and accurate electrochemical biosensor for the determination of glyphosate (GLY) in river and drinking water samples. The biosensor showed high reproducibility, excellent analytical performance, repeatability, and accuracy. It proves to be selective against other pesticides, organic acids, and inorganic salts.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Physical
Qing Liu, Kaiwei Wan, Yingxu Shang, Zhen-Gang Wang, Yiyang Zhang, Luru Dai, Chen Wang, Hui Wang, Xinghua Shi, Dongsheng Liu, Baoquan Ding
Summary: Self-assembling, histidine-rich peptides are capable of catalyzing H2O2 reduction reactions through the formation of periodic arrays of histidine residues, similar to haem-dependent peroxidases. These findings may have implications for the design of advanced biomimetic catalytic materials.
Article
Spectroscopy
Fatemeh Behrouzifar, Seyed-Ahmad Shahidi, Fereshteh Chekin, Shabnam Hosseini, Azade Ghorbani-HasanSaraei
Summary: A simple and sensitive colorimetric assay for hydrogen peroxide detection based on ABTS oxidation by UV-Vis spectroscopy was developed in this study. The HRP-rGO hybrid biosensor, with excellent detection limit and wide linear ranges, showed great potential for monitoring H2O2 in different beverages. The formation of green product with absorption peaks at 421, 655 and 737 nm was catalyzed by the HRP-rGO hybrid, which was confirmed by various characterization techniques.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Engineering, Environmental
Diego Alberto Morales Urrea, Analia Veronica Fernandez Gimenez, Yamila Eliana Rodriguez, Edgardo Martin Contreras
Summary: Immobilized HRP in calcium alginate beads showed efficient removal of azo-dye Orange II, especially in alkaline conditions and in the presence of hydrogen peroxide. A mathematical model was developed to predict and simulate experimental results under different conditions, with satisfactory validation against new data indicating high agreement between simulations and experiments.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Materials Science, Multidisciplinary
Jacopo Emilio Giaretta, Farshad Oveissi, Fariba Dehghani, Sina Naficy
Summary: The article presents a 3D printable paper-based sensor for detecting H2O2, which simplifies the fabrication process and detects H2O2 concentration by observing the change in resistance. The integration of enzyme enhances the detection performance of the sensor.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Chemistry, Analytical
Yunfei Zhang, Tingting Lin, Yi Shen, Hongying Li
Summary: In this study, a multi-scaled electrochemical biosensor was fabricated for the rapid detection of aflatoxin B1, showing exceptional performance due to the unique structures and synergistic effects of the components.
Article
Biochemistry & Molecular Biology
Yu Xiong, Chao Wang, YuanFei Wu, Chunhua Luo, Dongping Zhan, Shizhen Wang
Summary: Using two-dimensional metal-organic layers (MOLs) as electron transfer mediators helps to enhance the sensitivity, reproducibility, and stability of redox enzyme-based sensors.
Article
Chemistry, Inorganic & Nuclear
Shota Hagiwara, Atsuya Momotake, Takashi Ogura, Sachiko Yanagisawa, Akihiro Suzuki, Saburo Neya, Yasuhiko Yamamoto
Summary: The catalytic cycle of a peroxidase-mimicking heme-DNAzyme involves an intermediate known as compound I, formed through O-O bond cleavage of an Fe3+-bound hydroperoxo ligand. Peroxidase assaying was performed to elucidate the effects of heme electronic structure and local heme environment on catalytic activity. The study revealed that the activity of the heme-DNAzyme can be controlled independently through heme electronic structure and local environment, enhancing the understanding of the structure-function relationship.
INORGANIC CHEMISTRY
(2021)
Article
Engineering, Multidisciplinary
Christina G. Siontorou, Konstantinos N. Georgopoulos
Summary: The study introduces a peroxide biosensor based on a metal-supported, self-assembled lipid membrane incorporated with horseradish peroxidase, demonstrating good analytical and operational characteristics, as well as reliable performance in real water samples.
RESULTS IN ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Maciej Sosna, Elena E. Ferapontova
Summary: The oriented coupling of redox enzymes to electrodes by reconstitution onto redox cofactors poses the requirements for a suitable self-assembled monolayer (SAM) design. The kinetics of electron transfer in binary SAMs composed of alkanethiols and hemin-conjugated linker molecules depends on the SAM composition and hemin surface density. The use of SAM-modified electrodes with loosely dispersed redox centers and rigid molecular wires is important for the effective on-surface reconstitution of proteins and enzymes.
Article
Chemistry, Multidisciplinary
Xinghua Chen, Lufang Zhao, Kaiqing Wu, Hong Yang, Qing Zhou, Yuan Xu, Yongjun Zheng, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: Advances in nanoscience have led to the exploration of nanozymes as enzyme alternatives. This study found that the substrate selectivity of the peroxidase-mimic M-N-C can be significantly altered by forming bound intermediates with different metals, leading to superior selectivity compared to commonly used horseradish peroxidase.
Review
Chemistry, Multidisciplinary
Yaohua Gu, Siao Li, Mingming Li, Xinyu Wang, Ying Liu, Keren Shi, Xiaoyan Bai, Qing Yao, Zhiqiang Wu, Huiqin Yao
Summary: In recent years, photocatalytic reactions using graphitic carbon nitride (g-C3N4) as the catalyst and enzyme-catalyzed degradation using horseradish peroxidase (HRP) have shown great potential in degrading organic pollutants. The coupling effect of g-C3N4 and HRP improves the efficiency of carrier separation and generates more active species, leading to the solar-driven non-selective destruction of organic pollutants. This study provides a detailed description of the alteration of g-C3N4 and the immobilization of HRP, and outlines recent developments in the photo-enzyme coupling of g-C3N4 and HRP.
Article
Biotechnology & Applied Microbiology
Keren Shi, Xiangming Liang, Mingming Li, Zhenhua Zhai, Ying Liu, Xiaoyan Shan, Yang Ma, Qianqian Gan, Huiqin Yao
Summary: In this study, hydrogel films of poly(N,N-diethylacrylamide) (PDEA) were synthesized on glassy carbon (GC) electrodes to investigate the switchable electrochemical behavior of isoniazid (INH), an electroactive pharmaceutical molecule. The electrochemical response of INH on PDEA-modified electrodes was found to be sensitive to pH, temperature, and salt concentration, leading to the design and construction of a 3-input/8-output combinational pharmaceutical logic gate network and a 3-to-8 decoder. Furthermore, the relationship between the oxidation peak current (Ipa) and INH concentration (CINH) was studied using differential pulse voltammetry (DPV). This research provides a new approach and model for designing drug sensors and has potential applications in intelligent medical diagnostics and drug release.
SENSORS AND ACTUATORS REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Chaonan Wang, Huiqin Yao, Zidan Cai, Senkai Han, Keren Shi, Zhenglong Wu, Shulan Ma
Summary: The development of low-cost and high-efficiency iodine sorbents is essential for nuclear pollution control. In this study, we intercalated the tin sulfide cluster of [Sn2S6](4-) into Mg/Al-type layered double hydroxides to obtain Sn2S6-LDH, which exhibited highly efficient capture performance for iodine in both vapor and solution forms. The adsorption capacity of Sn2S6-LDH for iodine vapor reached 2954 mg/g, mainly through physisorption, while the sorption capacity for iodine in solutions achieved a significantly large value of 1308 mg/g. During iodine capture, the reduction of I-2 molecules to I- ions by S2- in [Sn2S6](4-) led to the formation of SnI4, where the amount of captured iodine was four times that of Sn. The excellent iodine capture capability of Sn2S6-LDH makes it a promising material for trapping radioactive iodine.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Haiqing Yan, Ruxin Deng, Siqi Zhang, Huiqin Yao, Jiacheng Duan, Hanchen Bai, Yongliang Li, Rong Liu, Keren Shi, Shulan Ma
Summary: In this study, Cd-doped Ni3S2 nanosheet arrays were fabricated via a hydrothermal strategy and Cd/Ni ratio tuning. The optimized Cd-Ni3S2/NF-1/4 exhibited the best electrocatalytic performance for water splitting, requiring ultralow overpotentials for both the hydrogen evolution reaction and oxygen evolution reaction. Furthermore, the Cd-doped Ni3S2 demonstrated improved overall water splitting performance due to enhanced electroconductivity and active sites accessibility. This work provides an effective strategy for the fabrication of highly efficient bifunctional electrocatalysts for hydrogen production.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Chemistry, Physical
Chaonan Wang, Zihuan Yu, Huiqin Yao, Ruoxi Jin, Keren Shi, Yongliang Li, Shulan Ma
Summary: This work reports on the development of inexpensive and efficient electrocatalysts for the hydrogen evolution reaction in water splitting. By growing triangular nanosheet arrays of O-doped Co/Fe disulfides on carbon cloth, an optimized sample exhibited outstanding electrocatalytic performance with strong durability and low overpotential. O doping effectively modulated the electronic structure and increased the intrinsic catalytic activity.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Chemistry, Physical
Yizhu Wang, Jiaqi Shi, Ruiqi Xiao, Cong Xiao, Huiqin Yao, Lingchen Meng, Hongyun Liu
Summary: In this study, metal halide perovskites were used to construct molecular logic gates. The CsPbBr3@SiO2 film electrode system showed different responses to external stimuli, such as Fe(III) and dopamine. Multiple logic circuits were successfully simulated, including a ternary CONSENSUS logic gate with a dual-optical signal output.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Analytical
Xinyu Wang, Wenzheng Ma, Mingming Li, Weisong Yan, Deyu Rao, Xiaoyan Bai, Keren Shi, Xihong Guo, Huiqin Yao
Summary: Binary hydrogel films based on GDY/PDEA were polymerized and assembled on PG electrode surface, and the CV signals of PHz were enhanced by electrocatalysis of GDY layer. The pH-sensitive property of the system was determined by the presence of protonated PHz, and the structural alterations of PDEA hydrogels were attributed to temperature and salt concentration-response. A multi-stimulus response hydrogel film environmental sensor was constructed, including logic gate systems and logic devices.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Physical
Zhenhua Zhai, Xiaoyan Shan, Mingming Li, Ying Liu, Feng Yang, Fuke Wang, Zhiqiang Tian, Keren Shi, Huiqin Yao
Summary: Poly(N',N-diethylacrylamide-co-4-vinylphenylboronic acid) (P(DEA-co-VPBA)) hydrogel films were successfully assembled on pyrolytic graphite (PG) electrode by radical polymerization, exhibiting reversible thermal, glucose and pH-triggered electrochemical reactions. The thermal response was attributed to PDEA, glucose sensitivity to VPBA, and pH sensitivity to both PDEA and VPBA. The hydrogel film electrodes showed enhanced multi-response cyclic voltammetry (CV) signals through the electrocatalytic oxidation of L-cysteine by FDA, enabling the construction of a 4-input/9-output binary logic circuit and other complex biological computing systems.
MOLECULAR CATALYSIS
(2023)
Article
Chemistry, Physical
Keren Shi, Yuren Luo, Guangyan Qian, Jiayu Luo, Mingming Li, Yuxiang Xiang, Huiqin Yao
Summary: In this study, Ag metal particles were introduced into CuFe2O4 and the oxygen vacancy concentration was increased. Different catalysts were prepared and tested, and it was found that 3% Ag-CuFe2O4 and 1% Ag-CuFe2O4-OVs showed the best performance. The effect of catalyst dosage and initial concentration of the solution on the catalytic efficiency was also investigated. The study concluded that the catalyst had good reusability.
SURFACES AND INTERFACES
(2023)
Article
Chemistry, Physical
Keren Shi, Guangyan Qian, Yueyue Kong, Mingming Li, Yuxiang Xiang, Huiqin Yao
Summary: The MCM-48/ZnFe2O4/BiOBr (MZBB) heterojunction photocatalyst was used to decompose rhodamine B (RhB) under visible light. The compound semiconductor degraded RhB by 99.03% after 60 min at a concentration of 40 mg/L and pH 6.0, with a total organic carbon (TOC) mineralization rate of 0.0631. The combination of n-type ZnFe2O4 and BiOBr improved the photocatalytic performance of the MCM-48/ZnFe2O4/ BiOBr composite, and the presence of n-n junctions promoted the separation of photogenerated carriers and prolonged recombination time.
SURFACES AND INTERFACES
(2023)
Article
Engineering, Environmental
Siao Li, Weisong Yan, Changyi Yang, Yanshu Zhu, Ying Liu, Yaohua Gu, Zhiqiang Wu, Keren Shi, Huiqin Yao
Summary: A novel photo-enzyme-coupled catalytic system was constructed by coupling horseradish peroxidase (HRP) with graphite carbon nitride (g-C3N4) through inorganic hybridization. The g-C3N4/HRP nanoflowers exhibited higher catalytic efficiency for the degradation of acridine, providing a promising platform for the degradation of acridine pollutants.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Review
Materials Science, Biomaterials
Yan Zu, Ziyu Wang, Huiqin Yao, Liang Yan
Summary: Radiotherapy (RT) is widely used in clinics and has advantages like strong penetration, high energy intensity, and low systemic side effects. However, tumor hypoxia during RT significantly reduces its therapeutic effect. Tumor hypoxia, a common characteristic of locally advanced solid tumors, promotes tumor cell proliferation, invasion, and metastasis. In recent years, the use of biocatalytic nanomaterials (BCNs) to reverse tumor hypoxia during RT has been explored. This review focuses on oxygen-generating BCNs and their potential in relieving tumor hypoxia in RT. The classification, engineering nanocatalytical activity of oxygen-generating BCNs, and combination therapy based on these BCNs are discussed. The challenges and prospects of using oxygen-generating BCNs in RT applications are summarized.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Materials Science, Biomaterials
Jiping Han, Yaohua Gu, Changyi Yang, Lingchen Meng, Runmei Ding, Yifan Wang, Keren Shi, Huiqin Yao
Summary: Nanozymes, nanomaterials with enzymatic activity, have been extensively studied by researchers. Single-atom nanozymes (SAzymes) with atomically dispersed active sites are leading the field of catalysis due to their exceptional performance. This review categorizes and describes SAzymes, explores methods for regulating their activity, and provides a comprehensive overview of the biosafety challenges associated with these enzymes.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Materials Science, Biomaterials
Jiping Han, Yaohua Gu, Changyi Yang, Lingchen Meng, Runmei Ding, Yifan Wang, Keren Shi, Huiqin Yao
Summary: Nanozymes, nanomaterials with enzymatic activity, have been extensively studied by researchers. Single-atom nanozymes (SAzymes) with atomically dispersed active sites are leading the field of catalysis due to their exceptional performance. This review categorizes and describes SAzymes based on their support type, explores methods for regulating their activity, and provides a comprehensive overview of the biosafety challenges associated with these enzymes.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Inorganic & Nuclear
Qian Wang, Lixiao Yang, Huiqin Yao, Zhenglong Wu, Rong Liu, Shulan Ma
Summary: This study demonstrates the excellent uranium capture ability of a ketoxime compound (DMG) intercalated magnesium-aluminum layered double hydroxide (DMG-LDH) at both high and low concentrations. The DMG-LDH shows a high sorption capacity and rapid sorption rate, making it an efficient material for uranium extraction from nuclear waste and seawater.
DALTON TRANSACTIONS
(2022)