Article
Biochemistry & Molecular Biology
Sagar Satpathi, Tamaki Endoh, Peter Podbevsek, Janez Plavec, Naoki Sugimoto
Summary: The study demonstrates detailed interaction analysis between PCs and RNAs to verify the impact of PCs on RNA functions, using berberine as a model PC. It identifies a minimal RNA motif interacting with berberine and highlights the importance of electrostatic and stacking interactions for sequence selective interaction and RNA stabilization. This selective interaction with a relatively small RNA motif based on a chemical structure of a planer heterocyclic highlights the biological activities of various PCs mediated by interactions with functional RNAs.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Plant Sciences
Nekpen Erhunse, Surekha Kumari, Pooja Anmol, Pooja Singh, Ehimwenma Sheena Omoregie, Agam Prasad Singh, Upendra Sharma, Dinkar Sahal
Summary: This study identified the specialized metabolites responsible for the antiplasmodial activity of Annickia affinis, finding that protoberberine alkaloids isolated from the plant showed excellent antiplasmodial activity, particularly against the blood-stage malaria parasite. Additionally, these compounds also exhibited promising activity against the liver-stage malaria parasite.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Engineering, Environmental
Ke Zhang, Joseph Hodge, Anamika Chatterjee, Tae Seok Moon, Kimberly M. Parker
Summary: Double-stranded RNA undergoes alkaline hydrolysis at orders-of-magnitude slower rates compared to single-stranded RNA, and can remain intact for months at neutral pH. Increasing pH effectively attenuates enzymatic degradation of dsRNA without inducing alkaline hydrolysis, which differs significantly from ssRNA. Understanding these unique properties of dsRNA allows for greater control of stability in RNAi technology and provides insights into various applications, including isolation, detection, and inactivation of dsRNA viruses.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Agronomy
Ningbo Chen, Xin Dai, Qian Hu, Hongxiao Tan, Liping Qiao, Laifeng Lu
Summary: Postharvest quality deterioration is a major issue in food loss and waste, but the technique of spray-induced gene silencing (SIGS) offers a new solution. By spraying double-stranded RNA (dsRNA) onto the surface of plants, specific genes can be targeted for RNA interference, effectively reducing browning in freshly-cut potatoes. This study highlights the potential application of SIGS in controlling postharvest quality deterioration.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Steven R. Strezsak, Penny J. Beuning, Nicholas J. Skizim
Summary: A method for accurately determining the concentration of long dsRNA was developed using nucleic acid digestion and reversed-phase HPLC/UV methods. The study found that sequence design can dramatically change the extinction coefficient of the molecule, with ssRNA overhangs leading to a 5% reduction in the calculated extinction coefficient.
ANALYTICAL METHODS
(2021)
Article
Biochemistry & Molecular Biology
Wenbo Xu, Miao Zhang, Yangcun Li, Wanwan He, Shengchun Li, Jiang Zhang
Summary: RNA interference (RNAi) is an effective technology for pest management. This study demonstrates that RNAi can be used to control the 28-spotted potato ladybird by targeting the beta-Actin gene. Transgenic potato plants expressing the target gene showed enhanced resistance to the pest. These findings expand the application range of RNAi technology to Coccinellidae pests.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Aben Ovung, A. Mavani, Ambarnil Ghosh, Sabyasachi Chatterjee, Abhi Das, Gopinatha Suresh Kumar, Debes Ray, Vinod K. Aswal, Jhimli Bhattacharyya
Summary: This study investigated the binding of well-known antibiotic sulfonamide drugs with heme protein myoglobin using various methods. The results showed the formation of a stable 1:1 complex between the drugs and myoglobin, with high binding constants. Drug binding led to a decrease in helicity and structural perturbation of myoglobin, indicating the dominance of nonpolyelectrolytic forces in the interaction. The binding distance suggested the possibility of energy transfer between the drugs and the protein. The destabilization of protein structure upon binding was also observed. Molecular docking identified the probable binding sites of the drugs within protein pockets.
Article
Microbiology
Alexander Stevens, Katherine Muratore, Yanxiang Cui, Patricia J. Johnson, Z. Hong Zhou
Summary: The atomic structure of TVV2 reveals the interactions driving capsid assembly and the properties governing viral genome organization. Comparison with other dsRNA viruses suggests conserved strategies of RNA release and potential domains involved in viral RNA maintenance. This provides insights for future investigations into the interactions between Trichomonas vaginalis and its viruses.
Article
Plant Sciences
Jinqiu Fu, Shijing Xu, Huan Lu, Fanchi Li, Shengchun Li, Ling Chang, David G. Heckel, Ralph Bock, Jiang Zhang
Summary: Plant-mediated RNA interference (RNAi) using dsRNA or siRNA has shown promise in pest control, but little is known about the molecular mechanisms. This study found that siRNAs derived from transgenic plants may be more effective triggers of RNAi in Lepidoptera than dsRNAs.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Agronomy
Danielle Duanis-Assaf, Ilya Shlar, Ortal Galsurker, Olga Davydov, Dalia Maurer, Oleg Feygenberg, Elena Poverenov, Robert Fluhr, Noam Alkan
Summary: By incorporating dsRNA into LDH nano-clays, the stability of dsRNA is improved and it can serve as a long-term controlled-release mechanism to selectively control postharvest pathogen B. cinerea. The LDH-dsRNA complex shows continued efficacy after long-term storage and reduces gray mold development significantly.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Bong Jung Kang, Zakea Sultana, Marcy N. Wilder
Summary: Vitellogenesis-inhibiting hormone (VIH) negatively regulates reproduction in shrimp, and transcriptional silencing of Liv-SGP-G was successfully achieved with dsRNA injections. Although the treatment did not promote ovarian maturation, it was the first report of co-transcriptional repression of Liv-SGP-G using dsRNAs for homologous genes. This study indicates that subadult shrimp can respond to transcriptional silencing techniques.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Danielle Duanis-Assaf, Ortal Galsurker, Olga Davydov, Dalia Maurer, Oleg Feygenberg, Moshe Sagi, Elena Poverenov, Robert Fluhr, Noam Alkan
Summary: Pathogenic fungi cause postharvest losses, prompting the need for new control strategies. The development of dsRNA-ERG effectively inhibits fungal growth and grey-mould development, reducing the required fungicide amount.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Bailing Ren, Jingnan Cao, Yanqiu He, Sheng Yang, Jiang Zhang
Summary: RNA interference is a efficient technology for pest control, and expression of dsRNAs in transplastomic plants may have potential effects on non-target pests. The study showed that ACT plants had cross-resistance to another coleopteran insect with highly conserved nucleotide sequence, providing an opportunity for simultaneous control of different pests.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Virology
Mi-Sun Yoo, A-Tai Truong, Hana Jeong, Do-Hyun Hahn, Ju-Seong Lee, Soon-Seek Yoon, So-Youn Youn, Yun-Sang Cho
Summary: Sacbrood virus (SBV) infection poses a significant threat to Apis cerana colonies in South Korea. RNA interference (RNAi) targeting the VP3 gene is an effective method for protecting and treating SBV in vitro and in infected colonies. Laboratory-based experiments and large-scale field trials demonstrate the safety and efficacy of VP3 double-stranded RNA (dsRNA) treatment in increasing survival rates and preventing symptomatic SBV infections.
Article
Biochemistry & Molecular Biology
Xiaoxiao Zhang, Peng Li, Huanhuan Zhong, Fanming Yang, Fanzhou Liu, Gabriel Yedid, Yan Zeng
Summary: Proteins involved in RNA metabolism possess a double-stranded RNA-binding domain (dsRBD), with variations in sequences and functions. A study showed that a short insertion in the dsRBD of Drosha is important for its function, and extending the L1 region in other dsRBDs reduces RNA binding. This highlights the sensitivity of dsRBDs to perturbations in the L1 region and adds to the understanding of their structural and functional diversity.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2021)
Article
Mechanics
Suresh S. Kumar, Arjun Shyam Sundar, R. Deepak
Summary: The stress intensity factor (SIF) of opposite surface cracks in an un-notched round bar under tension and combined load conditions was determined using a numerical method. The influence of loading modes on SIF was investigated, considering different crack depth ratios and crack aspect ratios. It was found that the SIF distribution changed as the crack depth ratio increased under pure tensile loading, and nonuniform and asymmetric SIF spread was observed when torque was applied. The effect of mode-II and mode-III fractures was more pronounced under combined loading.
MECHANICS BASED DESIGN OF STRUCTURES AND MACHINES
(2023)
Review
Rehabilitation
Yohali Burrola-Mendez, Sureshkumar Kamalakannan, Paula W. Rushton, Selsabil-A. Bouziane, Ed Giesbrecht, R. Lee Kirby, Rosemary J. Gowran, David F. Rusaw, Tomasz Tasiemski, Mary Goldberg, Marco Tofani, Jessica P. Pedersen, Jon Pearlman
Summary: This review summarized the research literature on wheelchair service provision education and found limited information on integrating wheelchair content into professional rehabilitation programs. It suggests prioritizing international partnerships, improving the quality and timeliness of training programs, and providing resources for educators to integrate wheelchair-related content.
DISABILITY AND REHABILITATION-ASSISTIVE TECHNOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Nurfaten Hamzah, Kamarul Imran Musa, Muhammad Hibatullah Romli, Xin Wee Chen, Mohd Zulkifli Abdul Rahim, Jafri Malin Abdullah, Mohd Azmi Suliman, Mohd Ismail Ibrahim, Tengku Alina Tengku Ismail, Iliatha Papachristou Nadal, Suresh Kumar Kamalakannan
Summary: The study aimed to translate and validate the CAFU instrument into Malay and test its validity and reliability among informal stroke caregivers in Malaysia. The results showed that the Malay-CAFU questionnaire had good relevancy, satisfactory item-level CVI, and high internal consistency. Therefore, the Malay-CAFU questionnaire is a valid and reliable instrument to assess the dependence level of stroke survivors and the upset level of informal stroke caregivers in Malaysia.
Review
Crystallography
Madunuri Chandra Sekhar, Eshwarappa Veena, Nagasamudram Suresh Kumar, Kadiyala Chandra Babu Naidu, Allam Mallikarjuna, Dudekula Baba Basha
Summary: This paper provides a review on recent advancements in piezoelectric materials and their applications in various fields such as catalysis, energy harvesting, and sensors. It also discusses the limitations of piezoelectric materials.
CRYSTAL RESEARCH AND TECHNOLOGY
(2023)
Article
Clinical Neurology
Sureshkumar Kamalakannan, Vijay Karunakaran, Ashwin Balaji, Aadi Sai Vijaykaran, Sivakumar Ramachandran, Ramakumar Nagarajan
Summary: ReWin, a digital therapeutics platform for stroke rehabilitation, was found to be feasible and acceptable in an Indian context through two phases of pilot testing. About 76% of participants used ReWin for more than half of the intervention period, and 90% of stroke care providers and 60% of stroke survivors and caregivers found the content of ReWin to be very relevant. Based on overall credibility, usability, and user-friendliness, 40% of stroke survivors and caregivers as well as 90% of stroke care providers rated the ReWin intervention as excellent.
FRONTIERS IN NEUROLOGY
(2023)
Review
Public, Environmental & Occupational Health
Tiago S. Jesus, Sureshkumar Kamalakannan, Sutanuka Bhattacharjya, Janet Prvu Bettger, Karen Jacobs, Helen Hoenig
Summary: This study describes a protocol for a mixed-methods systematic review aimed at identifying the usage and implementation of telerehabilitation in the delivery of rehabilitation services. The review will search six databases for relevant literature and use a framework synthesis approach to analyze the data. The results can inform future implementation efforts.
WORK-A JOURNAL OF PREVENTION ASSESSMENT & REHABILITATION
(2023)
Article
Health Policy & Services
Tiago S. Jesus, Karthik Mani, Sutanuka Bhattacharjya, Sureshkumar Kamalakannan, Claudia von Zweck, Ritchard Ledgerd
Summary: Occupational therapy workforce needs global strategy for strengthening, as there is a lack of research and resources, as well as various challenges and threats that need to be addressed.
INTERNATIONAL JOURNAL OF HEALTH PLANNING AND MANAGEMENT
(2023)
Article
Pediatrics
Manisha Shrestha, Gopal Bhandari, M. Optom, Sureshkumar Kamalakannan, Gudlavalleti Venkata Satyanarayana Murthy, Suresh Kumar Rathi, Anirudh Gaurang Gudlavalleti, Varun Agiwal, Hira Pant, Binod Pandey, Ramesh Ghimire, Daman Ale, Sajani Kayastha, Rakshya Karki, Daya Shankar Chaudhary, Raghunandan Byanju
Summary: This study aimed to evaluate the effectiveness of counseling and SMS/text messaging and phone calls to improve the follow-up rates among pediatric patients. The results showed that these interventions did not have a significant effect on improving the follow-up rate. However, the study provides useful insights for improving the follow-up rate among children with visual disabilities in Nepal and similar contexts.
JMIR PEDIATRICS AND PARENTING
(2023)
Review
Rehabilitation
Tiago S. Jesus, Karthik Mani, Claudia von Zweck, Sutanuka Bhattacharjya, Sureshkumar Kamalakannan, Ritchard Ledgerd
Summary: To fulfill their societal role, occupational therapists need to exist in sufficient supply, be equitably distributed, and meet competency standards. However, worldwide occupational therapy workforce research is scant and inequitably distributed, uses suboptimal methods, and is underfunded. Concerted efforts are needed to strengthen occupational therapy workforce research.
AMERICAN JOURNAL OF OCCUPATIONAL THERAPY
(2023)
Article
Chemistry, Physical
G. Velmurugan, Jasgurpreet Singh Chohan, S. A. Muhammed Abraar, R. Sathish, S. Senthil Murugan, M. Nagaraj, S. Suresh Kumar, V. Siva Shankar, D. Elil Raja
Summary: Natural fibre-based nanocomposite is a revolutionary material with excellent strength-to-weight ratio and lightweight, widely used in construction and dynamic industries. This study investigated the effects of silicon nanoparticles on the moisture absorption, mechanical properties, flammability, and dynamic mechanical behavior of PP/jute fibre composites. The results showed that increasing the weight of SiO2 nanoparticles improved the modulus of elasticity, tension strength, and elongation at breakage of the composites. The addition of SiO2 nanoparticles also enhanced the fire characteristics and dynamic mechanical behavior. However, the dispersion of SiO2 nanoparticles in the composites needs to be improved.
Article
Chemistry, Multidisciplinary
Rajapriya Pandy, Senthuran Suresh Kumar, Perumal Suresh, Jamespandi Annaraj, Mohan Pandi, Shanmugaiah Vellasamy, Suresh Sagadevan
Summary: In this study, seven endophytic fungi from Millingtonia hortensis were screened and five of them were found to produce taxol. The fungal taxol showed significant antibacterial and antifungal activities against human and fungal pathogens. Moreover, it exhibited cytotoxic effects against human liver cancer cells, suggesting its potential as an anticancer agent.
Meeting Abstract
Surgery
Joohyun Kim, Suresh Kumar, Yongqiang Yang, Seung-Keun Hong, Johnny Hong
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Review
Clinical Neurology
Dimple Dawar, Sureshkumar Kamalakannan, Nistara S. Chawla, Jibil T. Mathew, Esha Mehmood, Unnati Bhatnagar, Jeyaraj Durai Pandian
Summary: The study reviewed the intervention and outcome measures for post-stroke cognitive impairments. It found that there is diversity and heterogeneity in the interventions and measures used. While technology is replacing conventional approaches, there is limited data on scalable solutions. Future research is needed for evidence-based assessment and management of cognitive impairments in post-stroke rehabilitation.
ANNALS OF INDIAN ACADEMY OF NEUROLOGY
(2023)
Article
Chemistry, Applied
Jhilmil Swapnalin, Suresh N. Kumar, Kadiyala Chandra Babu Naidu, Prasun Banerjee
Summary: This review investigates the applications of Electron paramagnetic resonance (EPR) in biological systems, discussing its latest developments in areas such as photosynthesis, metalloproteins, radical enzymes, and phospholipid membranes. By utilizing EPR spectroscopy, the dynamics and structure of biological systems can be calculated, providing a better understanding of the radical state of proteins compared to XRD or NMR techniques.
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
(2023)
Review
Rehabilitation
Sureshkumar Kamalakannan, Paula W. Rushton, Ed Giesbrecht, David F. Rusaw, Selsabil-A Bouziane, Melodie Nadeau, Jennifer McKee, Rosemary J. Gowran, R. Lee Kirby, Jessica P. Pedersen, Tomasz Tasiemski, Yohali Burrola-Mendez, Marco Tofanin, Mary Goldberg, Jon Pearlman
Summary: This planned scoping review aims to examine and synthesize the global literature on wheelchair service provision education for healthcare professional students, healthcare personnel and educators. The review will provide insights into the organization, content, evaluation, and integration models of wheelchair service provision education on a global scale.
DISABILITY AND REHABILITATION-ASSISTIVE TECHNOLOGY
(2023)