期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 113, 期 36, 页码 12164-12168出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp9063559
关键词
-
资金
- Nano/Bio Science & Technology Program of MEST [2005-01325]
- KOSEF [RII-2008-052-02001-0, 2008-04306, 2007-8-1158]
- Korean Research Foundation [KRF-2005-005-J 13103]
- World Class University (WCU) program [R31-2008-000-10059-0]
Label-free and real-time detections of mismatched dsDNAs are demonstrated using MutS-protein-immobilized, single-walled carbon nanotube field effect transistor (SWNT-FET) devices. The E. coli MutS proteins specifically recognizing mismatched dsDNAs are immobilized on SWNT-FET devices that have been fabricated for high sensitivity using a shadow mask lithographic technique to obtain a thin and wide Schottky contact region. The MutS-immobilized SWNT-FETs have successfully detected 40 base pair dsDNAs having single G-T mismatches at the 20th base pair positions by displaying significant electrical conductance drops at as low as 100 pM concentration. Systematic control experiments have revealed that the signal changes indeed originated from specific recognitions of mismatched DNAs by the immobilized MutS proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据