期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 112, 期 6, 页码 1576-1579出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp711527v
关键词
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Laser-scanning coherent anti-Stokes Raman scattering (CARS) microscopy was used to image lipid domains in single bilayers without any labeling. On the basis of the molecular packing density difference between liquid-disordered (L-d) liquid-ordered (L-o), and gel (S-o) phases, clear vibrational contrasts were generated between coexisting domains in a single bilayer of DOPC/DPPC (1:1) and DOPC/DPPC/cholesterol (4:4:2). The method reported here can be potentially applied to study phase segregation in live cell membranes which are highly heterogeneous and dynamic.
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