Article
Plant Sciences
Mitja Krizman, Jernej Jakse
Summary: The wild Foeniculum vulgare populations from the region of Istria were studied through genetic and chemical analyses. Significant differences were observed in both chemical profiles and genetic traits among populations. The combined use of chemical and genetic analyses proved to be a more comprehensive approach for genetic variation analysis.
Article
Veterinary Sciences
D. Endrawati, E. Kusumaningtyas
Summary: This study compared the molecular profiles of Trichophyton mentagrophytes and Microsporum canis based on the digestion of ITS fragments with different restriction enzymes. The results showed distinct digestion products between the two fungi, suggesting a specific RFLP marker for species identification of dermatophytes fungi.
JURNAL ILMU TERNAK DAN VETERINER
(2021)
Article
Plant Sciences
Petr Koutecky, Jan Prancl, Jiri Kosnar, Eva Koutecka, Johana Hanzlickova, Magdalena Lucanova, Michaela Nejedla, Zdenek Kaplan
Summary: This study analyzed different populations within the water crowfoot genus using morphology, genome size, and genetic data, revealing genetic relationships and evolutionary processes among populations, as well as potential hybridization events and polyploidization within populations.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Plant Sciences
Jiao Zhang, Xiulian Chi, Juying Zhong, Alisdair Fernie, Saleh Alseekh, Luqi Huang, Dan Qian
Summary: This study found non-concerted evolution of internal transcribed spacer (ITS) sequences in the genus Lycium, showing high levels of polymorphism. The ITS paralogues were identified as pseudogenes or functional copies, with distinct differences from their corresponding functional copies. The results suggest a link between the non-concerted evolution of ITS and recent divergence of tandem repeats within the Lycium genome and hybridization between species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Infectious Diseases
John Asekhaen Ohiolei, Hong-Bin Yan, Li Li, Mughees Aizaz Alvi, Rosline James Muku, Yao-Dong Wu, Nian-Zhang Zhang, Wen-Hui Li, Ai-Min Guo, Xue-Lin Wang, Bao-Quan Fu, Wan-Zhong Jia
Summary: The study focused on the mitochondrial nad4-nad5 genes of T. hydatigena from China, Nigeria, Pakistan, and Sudan, identifying distinct mitochondrial lineages and genetic polymorphism. A PCR-RFLP assay was developed to differentiate between the two mitochondrial haplogroups, providing valuable molecular evidence for further exploration of the epidemiological significance of these different mitochondrial variants.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biochemical Research Methods
Laurentiu Benga, Eva Engelhardt, W. Peter M. Benten, Werner Nicklas, Martin Sager
Summary: This study developed specific PCR assays for five Rodentibacter species based on ITS region sequence differences, demonstrating 100% specificity and sensitivity against a diverse collection of Pasteurellaceae isolated from rats and mice and other strains. The PCR assays showed high limits of detection and successfully detected Rodentibacter spp. DNA in clinical swabs of laboratory mice and rats. These assays represent the first diagnostic tools for R. ratti, R. heidelbergensis, and a beta-haemolytic Rodentibacter taxon, facilitating better understanding of their distribution and biology in the future.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Microbiology
Fernando Martinez-Hernandez, Fabiola Sanchez-Aguillon, Joel Martinez-Ocana, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Eduardo Lopez-Escamilla, Pablo Maravilla, Guiehdani Villalobos
Summary: This study evaluated the genetic variability of the ITS region and pfor A gene of Trichomonas vaginalis from female patients and explored its implications in the host-parasite relationship. Results showed two main clusters in the ITS haplotypes and suggested that pfor A could be used as a marker for confirming trichomonads genus and species.
Article
Microbiology
Aisha Umar
Summary: Laccases have high biotechnological potential in industries due to their ability to catalyze the oxidation of various compounds. This study screened 10 Trichoderma species for laccase production, with T. atroviride showing the highest laccase production among them. Further analysis included purification, molecular weight determination, amino acid sequence confirmation, and phylogenetic tree construction of the laccase from T. atroviride.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Parasitology
Woo Jun Bang, Min Hyeok Won, Seong Tae Cho, Jihun Ryu, Kwang Shik Choi
Summary: This study developed a rapid and simple multiplex PCR assay for six morphologically similar Aedini species in Korea. By generating species-specific fragments distinguishable by size, accurate identification of mosquito species was achieved, providing a useful tool for monitoring and controlling these vector mosquitoes.
PARASITES & VECTORS
(2021)
Article
Food Science & Technology
Alice Giusti, Enrica Ricci, Laura Gasperetti, Marta Galgani, Luca Polidori, Francesco Verdigi, Roberto Narducci, Andrea Armani
Summary: This study aimed to build an ITS gene dataset to aid the Italian Health Service in mushroom identification. By retrieving and filtering ITS sequences from GenBank and BOLD databases, as well as collecting wild specimens, a dataset of 5977 sequences was successfully established to support regional poisoning cases.
Article
Mycology
Junichiro Hiruma, Honoka Nojo, Hitoshi Tsuchihashi, Hiromitsu Noguchi, Masataro Hiruma, Kazutoshi Harada, Rui Kano
Summary: In this study, T. interdigitale isolates were obtained from Japanese patients and their ITS types were investigated. The results showed that all 29 isolates belong to ITS type II, while one clinical isolate of T. mentagrophytes was similar to ITS type II*. Additionally, a terbinafine-resistant strain of T. interdigitale also belonged to ITS type II.
Article
Plant Sciences
Svetlana Goryunova, Anastasia Sivolapova, Oksana Polivanova, Evgeniia Sotnikova, Alexey Meleshin, Natalia Gaitova, Anna Egorova, Anatoly Semenov, Ekaterina Gins, Alina Koroleva, Evgeny Moskalev, Elena Oves, Oleg Kazakov, Aleksey Troitsky, Denis Goryunov
Summary: This study conducted a comparative analysis of chloroplast genomes in potato accessions with different cytoplasm types and estimated their time of origin. The research confirmed the presence of two main groups of chloroplast genomes among cultivated potato and revealed the genetic relationship among different cytoplasm types in potato.
Article
Immunology
Gobena Ameni, Alebachew Messele Kebede, Aboma Zewude, Musse Girma Abdulla, Rahel Asfaw, Mesfin Mamo Gobena, Martina Kyalo, Francesca Stomeo, Balako Gumi, Teshale Sori
Summary: This study conducted a phylogenetic analysis of Histoplasma capsulatum isolated from cases of epizootic lymphangitis (EL) in horses in central Ethiopia and evaluated their relationship with isolates from other countries and clades. By analyzing the internal transcribed spacer (ITS) region of rRNA genes, the study identified different clades of H. capsulatum and revealed that the Ethiopian isolates were closely related to isolates from Latin America and Eurasia, but distantly related to isolates from North America.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Agronomy
Mostafa Nafaa, Samah Mohamed Rizk, Tahany Abdel-Ghafar Ahmed Aly, Mohamed Abdel-Salam Rashed, Diaa Abd El-Moneim, Abir Ben Bacha, Mona Alonazi, Mahmoud Magdy
Summary: In this study, fungal diversity in newly reclaimed land in Egypt was investigated. The results showed that these fungi can enhance nutrient availability in the soil and produce the plant growth hormone IAA. The findings suggest the potential of developing fungal biofertilizers for improving agriculture reclamation processes and practices.
Article
Entomology
Su Ryeo Park, Do Eun Lee, Hwa Yeun Nam, Juil Kim, Si Hyeock Lee, Ju Hyeon Kim
Summary: Due to the morphological similarity and individual color variation of noctuid moth species, particularly at the larval stage, it is difficult to diagnose them in the field. In this study, we identified species-specific ITS1 sequences and developed two molecular species diagnosis protocols for efficient and accurate species identification. These protocols enable rapid and robust species diagnosis using a single multiplex PCR step, which is essential for proper management strategies.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Plant Sciences
Ni-Ni-Win, Takeaki Hanyuda, Aki Kato, Hiroshi Kawai
Article
Geography, Physical
Mayuri Inoue, Kotaro Shinmen, Hodaka Kawahata, Takashi Nakamura, Yasuaki Tanaka, Aki Kato, Chuya Shinzato, Akira Iguchi, Hironobu Kan, Atsushi Suzuki, Kazuhiko Sakai
GLOBAL AND PLANETARY CHANGE
(2012)
Article
Biotechnology & Applied Microbiology
Poh-Kheng Ng, Phaik-Eem Lim, Aki Kato, Siew-Moi Phang
JOURNAL OF APPLIED PHYCOLOGY
(2014)
Article
Environmental Sciences
Aki Kato, Mana Hikami, Naoki H. Kumagai, Atsushi Suzuki, Yukihiro Nojiri, Kazuhiko Sakai
MARINE ENVIRONMENTAL RESEARCH
(2014)
Article
Plant Sciences
Daphne Georgina Faria, Aki Kato, Shoichiro Suda
Article
Marine & Freshwater Biology
Aki Kato, Masasuke Baba, Shoichiro Suda
PHYCOLOGICAL RESEARCH
(2013)
Article
Environmental Sciences
M. C. Nash, B. N. Opdyke, U. Troitzsch, B. D. Russell, W. H. Adey, A. Kato, G. Diaz-Pulido, C. Brent, M. Gardner, J. Prichard, D. I. Kline
NATURE CLIMATE CHANGE
(2013)
Article
Plant Sciences
Aki Kato, Masasuke Baba
Article
Biotechnology & Applied Microbiology
Sakie Yoshioka, Aki Kato, Kazuhiko Koike, Noboru Murase, Masasuke Baba, Lawrence M. Liao
JOURNAL OF APPLIED PHYCOLOGY
(2020)
Article
Plant Sciences
Ni-Ni Win, Takeaki Hanyuda, Aki Kato, Hiromori Shimabukuro, Masayuki Uchimura, Hiroshi Kawai, Mutsunori Tokeshi
Summary: The taxonomic status and species diversity of the brown algal genus Padina were assessed based on DNA sequences and morpho-anatomy, revealing the presence of four distinct new species. The phylogenetic analyses provided insights into the biogeographic characteristics of Padina, with species in the Pacific Ocean showing shared distributions. Molecular time trees indicated an estimated divergence time for the genus Padina in the mid-Cretaceous.
JOURNAL OF PHYCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu Minamida, Hiroshi Matsuura, Takahiro Ishii, Kosuke Sato, Takashi Kamada, Aki Kato, Yukimasa Yamagishi, Tsuyoshi Abe, Norio Kikuchi, Minoru Suzuki
Summary: The chemical composition of three Laurencia species from the Seto Inland Sea of Japan has been examined, revealing the presence of distinct compounds in each species. Laurencia sp. and L. saitoi contain known compounds, while L. okamurae produces a unique compound characteristic to this species in Japan.
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
(2021)
Article
Plant Sciences
Aki Kato, Daniela Basso, Annalisa Caragnano, Graziella Rodondi, Line Le Gall, Viviana Pena, Jason M. Hall-Spencer, Masasuke Baba
Summary: After analyzing morpho-anatomical data and DNA sequences, it was discovered that the so-called "Lithophyllum okamurae" actually consisted of two species, Lithophyllum okamurae and a new cryptic species named L. neo-okamurae. These two species share similarities in morphology but can be distinguished by specific characteristics.
Article
Plant Sciences
Aki Kato, Narongrit Muangmai, Masasuke Baba
Summary: Using DNA sequence data and morpho-anatomical features, this study confirmed that the non-geniculate coralline alga Lithophyllum okamurae includes two infraspecific taxa, L. okamurae f. trincomaliense and L. okamurae f. validum, which should be treated as independent species. Morphologically, L. trincomaliense and L. validum are almost indistinguishable, with the shape of branch tips being the only distinguishable feature.
Article
Plant Sciences
Takashi Hosoi, Aki Kato
Summary: Geniculate coralline algae in temperate waters have dense, carpet-like growth and are commonly found in intertidal rocky habitats or beneath canopy-forming seaweeds in shallow subtidal zones. This study determined the optimal growth conditions for sporelings of two dominant geniculate species in temperate waters around Japan, Amphiroa cf. zonata and Corallina berteroi. The growth temperatures for these two species were comparable to temperate seaweeds commonly found in the same region, but their upper critical temperatures were higher than temperate kelps and similar to other macroalgae. Differences in irradiance effects on growth were related to the species' distributions in their local coastal environments.
Article
Ecology
S. Ohki, T. Irie, M. Inoue, K. Shinmen, H. Kawahata, T. Nakamura, A. Kato, Y. Nojiri, A. Suzuki, K. Sakai, R. van Woesik