期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
卷 235, 期 -, 页码 7-13出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2012.02.022
关键词
Carriers; Liposomes; Stability; Phthalocyanines; Singlet oxygen
资金
- University of Buenos Aires [X-063]
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP 104]
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 1030]
The lipophilic tetrasubstituted sensitizer 2,9(10),16(17),23(24)-tetrakis-(1-adamantylsulfanyl) phthalocyaninatozinc(II) (ZnPc) was incorporated into different liposomal formulations in order to evaluate its photophysical properties, as well as the size, incorporation efficiency, thermotropic properties and stability of liposomes during a time period. Liposomes were prepared by the thin-layer evaporation technique followed by hydratation with buffer at pH 7.4 and 0.15 M NaCl. Small unilamellar vesicles (SUV) were obtained after sonicating the mixture. Phthalocyanine was incorporated efficiently into all the liposomal formulations. The shape of ZnPc spectra depended on the environment. The S-PEG liposomal formulation showed the lowest aggregation value. The average diameter of liposomes with different lipid compositions varied from 49.70 +/- 0.09 to 223.10 +/- 0.36 nm, with significant differences between the various liposomal preparations. The inclusion of cholesterol (CHOL) and polyethylene glycol (PEG) on the lipid bilayer did not influence the size of the particles. The formulations with PEG increased the stability of liposomes for at least 9 months. After a long storage, the most stable formulations were those without CHOL. Singlet molecular oxygen values ranged between 0.10 and 0.26. These values were higher than other zinc(II) phthalocyanines incorporated into dimyristoyl phosphatidylcholine (DMPC) liposomes or Tween 80. (C) 2012 Elsevier B.V. All rights reserved.
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