Article
Biochemistry & Molecular Biology
Joseph V. W. Meeussen, Wim Pomp, Ineke Brouwer, Wim J. de Jonge, Heta P. Patel, Tineke L. Lenstra
Summary: The budding yeast TF Gal4 forms clusters that overlap with the GAL loci. Cluster number, density and size are regulated by the Gal4-inhibitor Gal80 and Gal4 concentration. Clustering aids TF recruitment to target genes, but non-DNA-bound Gal4 molecules inhibit transcription activation.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biology
Joel Frohlich, Pedro A. M. Mediano, Francesco Bavato, Alireza Gharabaghi
Summary: Pharmacological drug challenges are used to study the phenomenon of EEG delta oscillations persisting despite consciousness. Some drugs induce neural activity resembling cortical down states while participants remain conscious. These drugs can be valuable research tools for investigating the neural activity patterns necessary for consciousness.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ida Hoijer, Anastasia Emmanouilidou, Rebecka Ostlund, Robin van Schendel, Selma Bozorgpana, Marcel Tijsterman, Lars Feuk, Ulf Gyllensten, Marcel den Hoed, Adam Ameur
Summary: This study demonstrates that the use of CRISPR-Cas9 for genome editing can result in unintended off-target mutations that are heritable. These findings highlight the importance of pre-testing and assessing the risk of off-target effects in clinical applications.
NATURE COMMUNICATIONS
(2022)
Article
Cardiac & Cardiovascular Systems
Shiwei Qin, Meilin Xiang, Lei Gao, Xiaocheng Cheng, Dongying Zhang
Summary: This systematic review and meta-analysis found that higher serum uric acid (SUA) levels are associated with an increased risk of heart failure (HF) incidence, all-cause mortality, cardiac death, and HF rehospitalization in HF patients. Uric acid-lowering treatment was found to increase all-cause mortality in HF patients.
Article
Biochemistry & Molecular Biology
Kyle A. A. Sullivan, Iuliia Vitko, Kathryn Blair, Ronald P. P. Gaykema, Madison J. J. Failor, Jennifer M. M. San Pietro, Deblina Dey, John M. M. Williamson, Ruth L. L. Stornetta, Jaideep Kapur, Edward Perez-Reyes
Summary: Over a third of patients with temporal lobe epilepsy (TLE) are not effectively treated with current anti-seizure drugs, leading to the development of gene therapies. Injecting adeno-associated viral vectors (AAV) into the brain has been proven as a safe and feasible method. However, the regulation of AAV expression in therapeutic genes has not been achieved. This study demonstrates that drug-inducible gene therapies are effective in reducing spontaneous seizures and highlights the importance of evaluating the location and spread of AAV-based gene therapies in preclinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Tong Zhang, Ruowei Tang, Song Liu, Chao Chai, Yongsheng Chen, E. Mark Haacke, Shuang Xia
Summary: This study compared STAGE and hr-vwMRI for assessing MCA plaque vulnerability, finding that STAGE showed good efficiency. Patients with misery perfusion had different plaque characteristics compared to those without misery perfusion, with susceptibility and stenosis degree being independent predictors for the occurrence of misery perfusion.
EUROPEAN RADIOLOGY
(2021)
Article
Psychiatry
Megan Wiseman, Meagan Hinks, Darcy Hallett, Jacqueline Blundell, Ellen Sweeney, Christina M. Thorpe, Susan G. Walling, Ashlyn Swift-Gallant
Summary: Females are twice as likely as males to receive a diagnosis of post-traumatic stress disorder (PTSD). Findings from the study suggest that ovarian hormones contribute to the increased risk of PTSD in females, while lifestyle factors do not play a role in the risk of PTSD diagnosis.
JOURNAL OF PSYCHIATRIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xin Liu, Ye Zhang, Lucas D. Ward, Qinghong Yan, Tanggis Bohnuud, Rocio Hernandez, Socheata Lao, Jing Yuan, Fan Fan
Summary: A proteomics-based platform was developed to assess the abundance of off-target proteins, with 2813 proteins selected as the off-target proteome (SOTP) based on genetics and pharmacology evidence. Mass spectrometry was then used to identify 1828 proteins from the SOTP in 4 human cell lines.
SCIENTIFIC REPORTS
(2021)
Article
Surgery
Nikolaos Tsilimparis, Michel Bosiers, Timothy Resch, Giovanni Torsello, Martin Austermann, Fiona Rohlffs, Brandon Coates, Chyon Yeh, Tilo Koelbel
Summary: This study aimed to assess the clinical outcomes and target vessel patency of thoracoabdominal aortic aneurysms repair using the Zenith t-Branch Thoracoabdominal Endovascular Graft. The results showed durable repair with the t-Branch graft in patients treated for symptomatic or asymptomatic thoracoabdominal aortic aneurysms over a 2-year follow-up period.
JOURNAL OF VASCULAR SURGERY
(2023)
Article
Neuroimaging
Li-Mei Lin, Jonathan Collard de Beaufort, Alice S. Wang, Jessica K. Campos, David A. Zarrin, Benjamen M. Meyer, Geoffrey P. Colby, Alexander L. Coon
Summary: This study investigated the off-label use of the Comaneci device for improving vessel wall apposition in post-deployed flow diversion stents (FDS). The results showed that out of 74 cases, the Comaneci device was used in 22 cases (29.7%) to enhance vessel wall apposition. The deployment and retrieval of the Comaneci device were successful in all cases, with only 2 cases (9%) requiring subsequent balloon angioplasty.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Article
Microbiology
Menghao Yu, Youfu Zhao
Summary: Resistance to spectinomycin is caused by specific gene mutations, with ribosomal RNAs and protein biosynthesis playing critical roles. Additionally, cell-wall recycling and purine biosynthesis also contribute to spectinomycin resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Sabrina Traxel, Julia Lehmann, Stefanie Richard, Semjon Sidorov, Felix Niggli, Christoph Berger, David Nadal, Simone Burgler
Summary: B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most common childhood malignancy. Research suggests that Th-cell-induced AID expression may play a role in the pathogenesis, but in actual patients, the contribution of AID remains unclear.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Pharmacology & Pharmacy
Henri Leinonen, Cheng Cheng, Marja Pitkanen, Christopher L. Sander, Jianye Zhang, Sama Saeid, Teemu Turunen, Alyaa Shmara, Lan Weiss, Lac Ta, Timothy Ton, Ari Koskelainen, Jesse D. Vargas, Virginia Kimonis, Krzysztof Palczewski
Summary: CB-5083, an inhibitor of p97/valosin-containing protein (VCP), initially failed in cancer treatment due to adverse effects on vision, but shows potential for treating neuromuscular VCP disease. Research indicates that CB-5083 has a reversible inhibitory effect on retinal photoreceptor function, without long-term consequences on retinal function or structure.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Hinda Doucoure, Florence Auguy, Servane Blanvillain-Baufume, Sandrine Fabre, Marc Gabriel, Emilie Thomas, Fleur Dambreville, Coline Sciallano, Boris Szurek, Ousmane Koita, Valerie Verdier, Sebastien Cunnac
Summary: This study investigates the transcriptional activation of Xoo strains in rice. Genome editing can confer broad resistance on Asian Xoo strains but not on African Xoo strains. The study also identifies a possible off-target gene for TalC and documents the first example of divergent transcription activation by a native TALE during infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yawen Hu, Hang Gong, Ziyang Lu, Pengpeng Zhang, Sinian Zheng, Jing Wang, Binnian Tian, Anfei Fang, Yuheng Yang, Chaowei Bi, Jiasen Cheng, Yang Yu
Summary: In this research, the crucial role of SsGSR1, a glycosylphosphatidylinositol (GPI)-anchored cell wall protein, in the pathogenicity of S. sclerotiorum was characterized. SsGSR1 is involved in maintaining cell wall architecture and integrity, as well as inducing rapid cell death in host plants. Furthermore, allelic variants of SsGSR1 were found in field isolates, indicating the importance of genetic variation in SsGSR1 in the virulence of S. sclerotiorum.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Genmin Lu, Polly Pine, Janet M. Leeds, Francis DeGuzman, Pratikhya Pratikhya, Joyce Lin, John Malinowski, Stanley J. Hollenbach, John T. Curnutte, Pamela B. Conley
Article
Hematology
Michael P. Reilly, Uma Sinha, Pierrette Andre, Scott M. Taylor, Yvonne Pak, Francis R. DeGuzman, Nisha Nanda, Anjali Pandey, Moritz Stolla, Wolfgang Bergmeier, Steven E. McKenzie
Article
Biochemistry & Molecular Biology
Genmin Lu, Francis R. DeGuzman, Stanley J. Hollenbach, Mark J. Karbarz, Keith Abe, Gail Lee, Peng Luan, Athiwat Hutchaleelaha, Mayuko Inagaki, Pamela B. Conley, David R. Phillips, Uma Sinha
Article
Pharmacology & Pharmacy
Greg Coffey, Andreas Betz, Francis DeGuzman, Yvonne Pak, Mayuko Inagaki, Dale C. Baker, Stanley J. Hollenbach, Anjali Pandey, Uma Sinha
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2014)
Article
Pharmacology & Pharmacy
Stephen E. Spurgeon, Greg Coffey, Luke B. Fletcher, Russell Burke, Jeffrey W. Tyner, Brian J. Druker, Andreas Betz, Francis DeGuzman, Yvonne Pak, Dale Baker, Anjali Pandey, Stanley J. Hollenbach, Uma Sinha, Marc M. Loriaux
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2013)
Article
Pharmacology & Pharmacy
Greg Coffey, Francis DeGuzman, Mayuko Inagaki, Yvonne Pak, Suzanne M. Delaney, Dan Ives, Andreas Betz, Zhaozhong J. Jia, Anjali Pandey, Dale Baker, Stanley J. Hollenbach, David R. Phillips, Uma Sinha
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2012)
Article
Hematology
N Nanda, P Andre, M Bao, K Clauser, F Deguzman, D Howie, PB Conley, C Terhorst, DR Phillips
Article
Medicine, Research & Experimental
P André, SM Delaney, T LaRocca, D Vincent, F DeGuzman, M Jurek, B Koller, DR Phillips, PB Conley
JOURNAL OF CLINICAL INVESTIGATION
(2003)
Article
Multidisciplinary Sciences
V Ramakrishnan, F DeGuzman, M Bao, SW Hall, LL Leung, DR Phillips
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2001)
Article
Hematology
C Poujol, V Ramakrishnan, F DeGuzman, AT Nurden, DR Phillips, P Nurden
THROMBOSIS AND HAEMOSTASIS
(2000)