Article
Biochemistry & Molecular Biology
Adriana Elena Bulboaca, Alina Silvia Porfire, Vasile Rus, Cristina Ariadna Nicula, Corneliu Angelo Bulboaca, Sorana D. Bolboaca
Summary: This study aimed to evaluate the effect of liposomal epigallocatechin-gallate (LEGCG) compared with epigallocatechin-gallate (EGCG) solution on hepatic toxicity induced by gentamicin administration in rats. The results showed that LEGCG had better efficacy than EGCG in improving the oxidant/antioxidant balance, reducing inflammation, and protecting the liver from gentamicin-induced damage.
Article
Agriculture, Multidisciplinary
Yike Jiang, Ting Zheng, Weiping Jin, Yuxin Shi, Qingrong Huang
Summary: This study utilized chitosan and caseinophosphopeptides to improve the intestinal permeability of theaflavin-3,3'-digallate. The complexation between chitosan and caseinophosphopeptides was mainly driven by electrostatic interaction. The optimized nanocomplex successfully encapsulated theaflavin-3,3'-digallate and enhanced its intestinal permeability.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ayuni Yussof, Brian Cammalleri, Oluwanifemi Fayemiwo, Sabrina Lopez, Tinchun Chu
Summary: In this study, it was found that Theaflavin-3,3'-digallate (TFDG) has antibacterial and anti-spore activities, and can bind to bacterial proteins to reduce the expression of related genes. This suggests the potential application of TFDG as a broad-spectrum antibacterial and anti-spore agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Jinghui Zhou, Changwei Liu, Shimin Zhao, Ya Liu, Sheng Zhang, Qiang Zhao, Fen Wang, Gang Xu, Jianan Huang, Zhonghua Liu
Summary: Theaflavin-3,3'-digallate (TFDG) from black tea has various health benefits, but its application is limited due to low yields. In this study, tyrosinases from Bacillus megaterium were used to synthesize TFDG through directed evolution and high-throughput screening. Mutant Bmtyrc-3 showed significantly improved catalytic efficiency and higher production of TFDG. The broader substrate binding area of Bmtyrc-3 resulted in increased activity of substrates EGCG and ECG.
Article
Immunology
Yucheol Cheong, Minjin Kim, Jina Ahn, Hana Oh, Jongkwan Lim, Wonil Chae, Seung Won Yang, Min Seok Kim, Ji Eun Yu, Sanguine Byun, Yo Han Jang, Baik Lin Seong
Summary: The study investigated the potential use of catechins derived from green tea as adjuvants for subunit and inactivated vaccines, demonstrating their ability to enhance immune responses and provide protection against vaccines, including influenza virus.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Pengpeng Zhang, Wei Wu, Chunyu Du, Xiang Ji, Yaling Wang, Qiu Han, Hiaxia Xu, Cencen Li, Yongjie Xu
Summary: In this study, primary WAT and BAT SVF cells were isolated and induced to adipogenic differentiation in the presence or absence of EGCG. RNA-seq was used to analyze the gene regulation by EGCG in these two distinct adipose tissues and identify the key differences. The findings of this study have significant implications for obesity-related clinical and basic research.
Article
Chemistry, Applied
Jianyong Zhang, Hongchun Cui, Jiahuan Qiu, Xiaoqing Wang, Yixin Zhong, Caiping Yao, Lanying Yao, Qunxiong Zheng, ChunHua Xiong
Summary: This study aimed to improve the stability and bioavailability of EGCG in aqueous solutions and in vivo by preparing EGCG glycosylated arachin nanoparticles and EGCG glycosylated casein nanoparticles. The results showed that the DPPH scavenging activity of the nanoparticles was stable within temperature range of 25-70 degrees Celsius. EGCG enhanced the crosslinking effect of molecular particles in the glycosylation complexes solution. The glycosylated protein nanoparticles were stable to acid-base and enzymolysis in simulated gastrointestinal fluid.
Article
Biochemistry & Molecular Biology
Ying Huang, Changzheng Gao, Wei Song, Wanqing Wei, Xiulai Chen, Cong Gao, Jia Liu, Jing Wu, Liming Liu
Summary: In this study, a microbial polyphenol oxidase from Bacillus megaterium was used to synthesize theaflavin-3,3 '-digallate (TFDG). By optimizing the conformation of the O-O bond dissociation, the catalytic efficiency of the enzyme was improved. The optimized conditions resulted in a significantly higher production of TFDG, indicating the great potential of this method in the synthesis of TFDG and other TFs.
Article
Immunology
Lei Zhang, Wenming Li, Zhenyang Hou, Zhidong Wang, Wei Zhang, Xiaolong Liang, Zerui Wu, Tianhao Wang, Xin Liu, Xiaole Peng, Xing Yang, Huilin Yang, Dechun Geng
Summary: By modulating autophagy, Theaflavin-3,3'-digallate (TFDG) can alleviate inflammation and promote anti-inflammatory M2 macrophage polarization, thus protecting joints against collagen-induced arthritis.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Plant Sciences
Qianjin Xie, Xiaqiang Cai, Xu Dong, Ying Wang, Minghui Sun, Lingling Tai, Yan Xu
Summary: The combination of EGCG with Vc and glycerol could effectively increase its stability and uric acid-lowering activity, which sheds light on the development and application of EGCG in the fields of food and medicine.
PHARMACEUTICAL BIOLOGY
(2021)
Article
Chemistry, Applied
Xia Yu, Xinghong Cai, Shuang Li, Liyong Luo, Jie Wang, Min Wang, Liang Zeng
Summary: By utilizing various experimental techniques such as fluorescence spectroscopy and UV-vis absorption spectroscopy, the interaction mechanism between theaflavin-3,3'-digallate (TFDG) and bovine serum albumin (BSA) was studied, demonstrating that hydrophobic interactions and hydrogen bonds play a dominant role in their interaction. This research provides the possibility for further development of nanoparticles with superior biochemical properties and potential exploration of BSA as a transport carrier for TFDG.
Article
Chemistry, Applied
Xin Luo, Jiankang Lu, Yongyan Wu, Wenshan Duan, Fengping An, Qun Huang, Lei Chen, Shaofeng Wei
Summary: The potential allergenicity of amandin was reduced by binding it with EGCG using different methods, such as alkaline, free radical, ultrasound-assisted alkaline, and ultrasound-assisted free radical methods. The binding of amandin to EGCG was found to be covalent or non-covalent, and was mainly through cysteine and glutamate residues. This study provides a new method and theoretical basis for developing hypoallergenic almond food.
Article
Chemistry, Applied
Qing-Qing Ye, Gen-Sheng Chen, Weichun Pan, Qing-Qing Cao, Liang Zeng, Jun-Feng Yin, Yong-Quan Xu
Summary: The study focused on the contribution of EGCG to astringency, the impact of mucin as a protein, and developed a predictive model to estimate the intensity of EGCG astringency.
Article
Agriculture, Multidisciplinary
Han Peng, Fereidoon Shahidi
Summary: In this study, ester derivatives of epigallocatechin gallate (EGCG) were synthesized and their antioxidant efficacy was evaluated. The results showed that the lipophilicity of the EGCG derivatives increased with the increasing chain length of the acyl group, which affected their antioxidant performance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Applied
Takashi Tanaka, Miho Yasumatsu, Mayu Hirotani, Yosuke Matsuo, Na Li, Hong-Tao Zhu, Yoshinori Saito, Kanji Ishimaru, Ying-Jun Zhang
Summary: This study investigated the reaction of Theanaphthoquinone (TNQ) with epigallocatechin-3-O-gallate (EGCg) in black tea leaves, revealing the formation of novel reaction products and providing new insights into the formation of black tea pigment thearubigins.