期刊
JOURNAL OF PHARMACOLOGICAL SCIENCES
卷 117, 期 2, 页码 106-115出版社
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.11118FP
关键词
NK1 receptor; NK2 receptor; NK3 receptor; human colon; smooth muscle
In this study, we attempted to clarify the mechanism of tachykinin-induced motor response in isolated smooth muscle preparations of the human colon. Fresh specimens of normal colon were obtained from patients suffering from colonic cancer. Using mucosa-free smooth muscle strips, smooth muscle tension with circular direction was monitored isometrically. Substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) produced marked contraction. All of these contractions were inhibited by saredutant, a selective NK2-R antagonist, but not by CP122721, a selective NK1-R antagonist or talnetant, a selective NK3-R antagonist. beta Ala(8)-NKA(4-10) induced concentration-dependent contraction similar to NKA, but Sar(9)-Met(11)-SP and Met-Phe(7)-NKB did not cause marked contraction. Colonic contraction induced by beta Ala(8)-NKA(4-10) was completely blocked by saredutant, but not by atropine. Tetrodotoxin or N-G-nitro-L-arginine methyl ester pretreatment significantly enhanced beta Ala(8)-NKA(4-10) induced contraction. lmmunohistochemical analysis showed that the NK2-R was expressed on the smooth muscle layers and myenteric plexus where it was also co-expressed with neuronal nitric oxide synthase in the myenteric plexus. These results suggest that the NK2-R is a major contributor to tachykinin-induced smooth muscle contraction in human colon and that the NK2-R-mediated response consists of an excitatory component via direct action on the smooth muscle and an inhibitory component possibly via nitric oxide neurons.
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