Article
Pharmacology & Pharmacy
Donghui Lao, Rong Liu, Jianying Liang
Summary: By applying metabolomics, potential biomarkers for AIDS patients have been identified and verified, suggesting their potential role in the diagnosis of HIV/AIDS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Asmaa A. El Zaher, Ehab F. El Kady, Hussein M. El Messiry, Hind E. El Ghwas, Ola M. El Houssini
Summary: The study evaluated the bioavailability and pharmacokinetic properties of pantoprazole/amitriptyline in rabbit plasma using LC-MS/MS method. Effervescent granules formulation showed superior pharmacokinetic parameters in rabbits compared to marketed products.
Article
Medicine, Research & Experimental
Ni Luo, Ming Sun, Xuhua Han, Linling Li, Lei Wang, Zeneng Cheng
Summary: Mefunidone (MFD), a derivative of pirfenidone, showed promising anti-fibrosis and anti-inflammatory activity in preclinical evaluations. The metabolization of MFD mainly occurs through oxidation, N-demethylation, and hydroxylation pathways. Importantly, MFD did not induce or inhibit metabolic enzymes in preclinical investigations, supporting further clinical research on MFD.
Article
Chemistry, Analytical
Lanyu Liu, Qian Liu, Yan Wang, Yu Yuan, Feifan Xie
Summary: This study aimed to evaluate different combinations of polyanion-metal ion based solution system for phospholipids removal and elimination of matrix effects in human plasma. The optimized ZrOCl2-Ludox/DSS systems provided efficient phospholipids removal and adequate analyte recovery, and they also correctly eliminated ion suppression or enhancement of the non-polar and polar drugs.
ANALYTICA CHIMICA ACTA
(2023)
Article
Pharmacology & Pharmacy
Pengfei Li, Jun Peng, Yuexin Li, Lili Gong, Yali Lv, He Liu, Tianhong Zhang, Song Yang, Hongchuan Liu, Jinglai Li, Lihong Liu
Summary: This study systematically evaluated the pharmacokinetics, bioavailability, metabolism, and excretion of ASD in rats for the first time. The ultra-low oral bioavailability of ASD was confirmed to be due to poor gastrointestinal permeability, extensive pre-absorption degradation, and biotransformation. In addition to being excreted in urine and bile, ASD possibly undergoes complex metabolic elimination after intravenous administration.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Yang Tang, Michael Van Parys, Abigail Walker, Alexandra Drelick, Xiaorong Liang, Brian Dean, Liuxi Chen
Summary: In this study, a LC-MS/MS surrogate matrix assay was developed for accurate urea quantitation in various biological matrices. The assay demonstrated reliable and robust performance, meeting bioanalysis requirements for clinical studies. The potential of the assay for urea quantitation in other physiological matrices was also explored.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Biochemical Research Methods
Runcong Guo, Jiafeng Hu, Jiao Jing, Ying Liu, Jian Li, Yu Zhou, Hong Liu, Lei Zhou, Xiaoyan Chen
Summary: The LC-MS/MS method developed in this study provides a sensitive and reliable way to analyze the pharmacokinetics of fluoropezil and its metabolites in human plasma.
Article
Biochemical Research Methods
Ehab F. Elkady, Bushra M. Jaadan, Elsayed Ramadan, Ayman Abo Elmaaty
Summary: This study aimed to estimate atorvastatin (ATV) and ezetimibe (EZE) simultaneously in the presence of ATV major and active metabolites using a validated LC-MS/MS method. The proposed method exhibited good linearity (r(2) > 0.99), accuracy (92.02-109.94%), and precision (CV% <= 14) for ATV, EZE, 2-hydroxy ATV, and 4-hydroxy ATV. The applied liquid-liquid extraction resulted in reliable extraction recoveries for ATV, EZE, 2-hydroxy ATV, and 4-hydroxy ATV.
Article
Biochemistry & Molecular Biology
Kunqian Mu, Kaiwen Jiang, Yue Wang, Zihan Zhao, Song Cang, Kaishun Bi, Qing Li, Ran Liu
Summary: A LC-MS/MS method was developed to study the in vivo fate of beta-cyclodextrin. The results showed that beta-cyclodextrin has a wide distribution in rats' bodies and is rapidly excreted, indicating low accumulation and good safety.
Article
Biochemistry & Molecular Biology
Alessandro Di Giorgi, Giorgia Sprega, Lourdes Poyatos, Esther Papaseit, Clara Perez-Mana, Annagiulia Di Trana, Maria Rosaria Vari, Francesco Paolo Busardo, Simona Pichini, Simona Zaami, Alfredo Fabrizio Lo Faro, Magi Farre
Summary: The aim of this study was to determine the excretion of methylone and its metabolites in sweat after controlled doses of 50, 100, 150, and 200 mg of methylone were ingested by twelve healthy volunteers in a clinical trial. Methylone and its metabolites, 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone (MDC), were detected in sweat patches using liquid chromatography-tandem mass spectrometry. Methylone and MDC were found in sweat after 2 hours and reached their highest concentration at 24 hours following the administration of doses. However, HMMC was not detectable at any time interval. Sweat has proven to be a suitable matrix for determining the concentration of methylone and its metabolites in clinical and toxicological studies, indicating recent drug consumption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Tanja R. Zijp, Tim J. Knobbe, Kai van Hateren, Jan Roggeveld, Hans Blokzijl, C. Tji Gan, Stephan J. L. Bakker, Erwin M. Jongedijk, Daan J. Touw
Summary: A straightforward and rapid method was developed for determination of plasma tacrolimus in solid organ transplant recipients, using liquid chromatography tandem mass spectrometry (LC-MS/MS). This method is suitable for measurement of tacrolimus in plasma and will facilitate ongoing observational and prospective studies on the relationship of plasma tacrolimus concentrations with clinical outcomes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lea Wagmann, Aline C. Vollmer, Lucas Lauder, Felix Mahfoud, Markus R. Meyer
Summary: The study aimed to monitor adherence to antihypertensive medication by developing a quantification procedure for drug concentrations in blood plasma. Results showed that plasma concentration interpretation is more complex than urine screening but can effectively assess adherence. Further data is needed to determine if plasma quantification is superior in assessing adherence to antihypertensive medication.
Article
Biochemical Research Methods
Kaiyue Ren, Yanjuan Yuan, Qing Shao, Hongqun Qiao
Summary: A highly sensitive method for simultaneous determination of [F-19]-alfatide and alfatide using liquid chromatography/tandem mass spectrometry was developed and validated. The method showed high specificity, precision, and accuracy, with an extended linear range. It was successfully applied to the pharmacokinetic study of [F-19]-alfatide in rats.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Pharmacology & Pharmacy
Tianming Ren, Runzhi Li, Liqiang Zhao, J. Paul Fawcett, Dong Sun, Jingkai Gu
Summary: An innovative bioassay was developed to simultaneously quantify TPGS and its metabolite PEG1000, and it was used to investigate the pharmacokinetics, tissue distribution, and excretion of TPGS and PEG1000 in rats. The results showed that TPGS had poor oral absorption and low bioavailability, and after intravenous administration, TPGS and PEG1000 were mainly distributed in the spleen, liver, lung, and kidney and slowly eliminated in urine and feces as PEG1000. Additionally, TPGS only weakly inhibited CYP3A4 activity in human liver microsomes.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Analytical
Josefine Schulz, Robin Michelet, Jan F. Joseph, Markus Zeitlinger, Fabian Schumacher, Gerd Mikus, Charlotte Kloft
Summary: The pharmacokinetics of Voriconazole (VRC) is not fully understood, posing challenges for its therapeutic use. A bioanalytical LC-MS/MS assay was developed and validated to simultaneously quantify VRC and its metabolite in human plasma, ultrafiltrate, and microdialysate. The assay demonstrated accuracy and precision, and its suitability was shown in clinical and in vitro experiments. This method allows for increased sampling in vulnerable populations and provides a higher temporal resolution in microdialysis studies, which contributes to a better understanding of VRC's pharmacokinetics and therapy optimization.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)