Review
Chemistry, Analytical
Yuan Su, Longjiao Zhu, Yifan Wu, Zihong Liu, Wentao Xu
Summary: This paper summarizes the factors influencing aptamer performance during the SELEX process and provides an advanced screening strategy for aptamers against bacterial whole-cell components. The binding sites between aptamers and bacterial whole-cell components are examined, and sequence sites are analyzed from an aptamer perspective. Additionally, the application status and prospects of bacterial aptamers are briefly described.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Genetics & Heredity
Shahnawaz A. Baba, Shubham Jain, Naveen K. Navani
Summary: A new colorimetric method for monitoring the progress of nucleic acid aptamer selection is described, utilizing rolling circle amplification (RCA) and the activity of G-quadruplex/hemin DNAzyme. The method provides a quantitative measurement of SELEX progress by analyzing the signal generated by DNA pool bound to their target.
Review
Biotechnology & Applied Microbiology
Yixin Xu, Xin Jiang, Yanhong Zhou, Ming Ma, Minjin Wang, Binwu Ying
Summary: This review provides a comprehensive overview of the latest development of SELEX technology and focuses on the applications of aptamer-based technologies in infectious diseases, such as targeted drug-delivery, treatments, and biosensors for diagnosing. The challenges and the future development in this field of clinical application will also be discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Yujing Ding, Zihan Gao, Hao Li
Summary: A novel real sample assisted SELEX selection strategy was proposed to select aptamers with high detection performance, using SAR specific aptamer Apt-F2 as an example, which showed higher specificity and SAR binding affinity compared to Apt-B4 selected by standard SELEX. The aptasensors developed based on Apt-F2 demonstrated potential for detecting SAR in real milk or other animal-sourced food samples, with slightly superior detection limits and recovery rates compared to those based on Apt-B4.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Biology
Jonghoe Byun
Summary: Aptamers, short nucleic acid sequences capable of specific molecular binding, have shown significant potential in therapeutics and diagnostics. Despite challenges in clinical trials, novel technologies and advancements are fueling hope in the field of aptamers.
Review
Nanoscience & Nanotechnology
Shuaijian Ni, Zhenjian Zhuo, Yufei Pan, Yuanyuan Yu, Fangfei Li, Jin Liu, Luyao Wang, Xiaoqiu Wu, Dijie Li, Youyang Wan, Lihe Zhang, Zhenjun Yang, Bao-Ting Zhang, Aiping Lu, Ge Zhang
Summary: Aptamers, with oligonucleotide sequences, have capabilities to bind to specific target molecules and are attracting great attention in diverse clinical translations. They offer advantages such as prolonged storage life and feasibility of chemical modifications for enhancing stability.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Binfen Chen, Xinmei Yu, Ting Gao, Yaoyao Wu, Xiaojun Zhang, Sanshu Li
Summary: Aptamers are RNA or DNA molecules that can bind to ligands and have various applications such as biosensors, diagnostic tools, and therapeutic agents. The traditional method of selecting aptamers and integrating an expression platform has limitations, but this study introduces the use of allosteric DNAzymes known as aptazymes to overcome these drawbacks. The researchers used the Expression-SELEX technique to select aptazymes specifically activated by low concentrations of l-phenylalanine. Three aptazymes were characterized and found to have a high affinity for l-phenylalanine, improved catalytic rate in its presence, and the ability to discriminate against similar analogs. This work demonstrates the effectiveness of Expression-SELEX in enriching high-quality ligand-responsive aptazymes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Electrochemistry
Geanina Stefan, Oana Hosu, Karolien De Wael, Maria Jesus Lobo-Castanon, Cecilia Cristea
Summary: Aptamers have emerged as bio-mimetic molecular recognition elements in biomedicine, with various applications in diagnostics, drug delivery, therapeutics, and pharmaceutical analysis. Despite their advantages, there are challenges to be addressed, such as poor specificity and sensitivity, questionable clinical use, low drug loading, in vivo stability, and toxicity. Further technological advances and research are needed to fully realize the potential of aptamers in the biomedical field.
ELECTROCHIMICA ACTA
(2021)
Review
Biochemistry & Molecular Biology
Su Jin Lee, Junmin Cho, Byung-Hoon Lee, Donghwan Hwang, Jee-Woong Park
Summary: This article introduces the use of computational simulation methods to develop aptamers and minimize the workload required for screening and optimizing aptamers. Various methods, including conformational structure prediction, molecular docking, and molecular dynamic simulation, were investigated for the estimation of aptamer-target molecule binding. In addition, examples of machine learning and deep learning technologies used to predict the binding of targets and ligands in the development of new drugs are introduced. This review will be helpful in the development and application of in silico aptamer screening and characterization.
Article
Multidisciplinary Sciences
Alex M. Yoshikawa, Leighton Wan, Liwei Zheng, Michael Eisenstein, H. Tom Soh
Summary: This study presents a high-throughput screening process for generating high-specificity aptamers to multiple targets without the need for counterselection. The process can be easily adapted to different small-molecule analytes, accelerating the development of aptamer reagents with high specificity for target analytes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Junku Takao, Reina Nagai, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi
Summary: This study introduces a novel aptamer selection method based on microscale electrophoretic filtration, which enables simple operations, highly effective separation, and the use of relatively inexpensive apparatus.
Article
Chemistry, Analytical
Lingli Jiang, Min Wang, Yi Zhang, Haihua Chen, Yi Su, Yiyi Wang, Jun Sheng Lin
Summary: The study utilized capture-SELEX to select aptamers against roxithromycin and developed a colorimetric aptasensor for its detection, showing high affinity and specificity. The research laid a foundation for detecting roxithromycin in actual aquatic environments.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Changxin Li, Mingqian Song, Shijia Wu, Zhouping Wang, Nuo Duan
Summary: This study describes the screening strategy of LEV aptamers using Capture-SELEX and the construction of a dual-mode aptasensor for LEV detection. The aptamers showed good binding affinity and specificity, and the aptasensor demonstrated high sensitivity for LEV detection in milk.
Review
Cell Biology
Yang Liu, Nijia Wang, Chiu-Wing Chan, Aiping Lu, Yuanyuan Yu, Ge Zhang, Kangning Ren
Summary: Aptamers, single-strand oligonucleotides with binding capability to specific target molecules, are increasingly used in analysis, diagnosis, and medical treatment. However, conventional SELEX methods have limitations, leading to restricted applications of aptamers. Microfluidic technologies offer promise for rapid and high throughput aptamer selection due to their low cost, high speed, and automation advantages.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Chemistry, Analytical
Chen Lyu, Imran Mahmood Khan, Zhouping Wang
Summary: The emerging aptamer through SELEX, especially Capture-SELEX for small molecule selection, has shown significant potential, but also faces limitations that require further research and improvement.
Review
Biochemistry & Molecular Biology
Patricia de Carvalho Ribeiro, Lucas Felipe Oliveira, Daniel Mendes Filho, Ricardo Cambraia Parreira, Mariana Sousa Vieira, Bruno Lemes Marques, Elis Marra da Madeira Freitas, Walison N. N. Silva, Helton da Costa Santiago, Alexander Birbrair, Henning Ulrich, Valdo Jose Dias da Silva, Rodrigo R. R. Resende
Summary: Systemic arterial hypertension is a major risk factor for cardiovascular and renal diseases, and early recognition is important for prevention. The study of biomarkers is an important approach for diagnosing and predicting hypertension and related conditions. This review discusses the use of biomarkers in hypertension and associated diseases such as ischemic stroke, intracerebral hemorrhage, transient ischemic attack, acute myocardial infarction, angina pectoris, and chronic kidney disease. Establishing a pool of potential biomarkers can improve diagnosis, prognosis, risk assessment, therapy management, and pharmacological responses to enhance patients' quality of life and prevent unfavorable outcomes.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Physiology
Marcela Guimaraes Takahashi De Lazari, Celso Tarso Rodrigues Viana, Luciana Xavier Pereira, Laura Alejandra Ariza Orellano, Henning Ulrich, Silvia Passos Andrade, Paula Peixoto Campos
Summary: The study found that sodium butyrate can attenuate the inflammatory, angiogenesis, and fibrogenesis processes involved in peritoneal fibrosis, indicating its potential for improving the condition. This finding contributes to the development of new medications for treating peritoneal fibrosis.
EXPERIMENTAL PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Cecilia Villegas, Ivan Gonzalez-Chavarria, Viviana Burgos, Hector Iturra-Beiza, Henning Ulrich, Cristian Paz
Summary: This review summarizes the preclinical and clinical studies on the anticancer effects of epothilones from 2010 to 2022. Epothilones exhibit similar mechanisms of action to paclitaxel, inducing tubulin polymerization and apoptosis, and have low susceptibility to tumor resistance mechanisms. They are more effective than paclitaxel in combating refractory tumors. While most synthesized epothilone derivatives have failed in clinical trials, ixabepilone and utidelone are currently used in clinical practice. There is robust evidence that ixabepilone plus capecitabine or utidelone in combination with capecitabine improves the treatment of triple-negative breast cancer (TNBC). In recent years, innovative synthetic strategies have led to the development of new epothilone derivatives with improved activity against refractory tumors, surpassing ixabepilone and taxol. These compounds, together with specific delivery mechanisms, hold promise for the development of anticancer drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Karina Scheuermann, Celso Tarso Rodrigues Viana, Diego Carlos dos Reis, Marcela Guimaraes Takahashi de Lazari, Laura Alejandra Ariza Orellano, Clara Tolentino Machado, Leticia Cristine Cardoso dos Santos, Henning Ulrich, Luciano Santos Aggum Capettini, Silvia Passos Andrade, Paula Peixoto Campos
Summary: The effects of amitriptyline (AM) on foreign body reaction (FBR) after biomedical material implantation are not known. This study found that AM can inhibit early angiogenesis and fibrogenesis of the implants, but has no significant effects on 7-day-old implants.
Article
Neurosciences
Deidiane Elisa Ribeiro, Lyvia Lintzmaier Petiz, Talita Glaser, Agatha Oliveira-Giacomelli, Roberta Andrejew, Fernando de Azevedo Ribeiro Saab, Milena da Silva Milanis, Henrique Correia Campos, Vanessa Fernandes Arnaud Sampaio, Sophia La Banca, Beatriz Monteiro Longo, Claudiana Lameu, Yong Tang, Rodrigo Ribeiro Resende, Sergio T. Ferreira, Henning Ulrich
Summary: About 10 million new cases of dementia occur globally each year, with up to 70% attributed to Alzheimer's disease (AD). AD patients often experience non-cognitive symptoms known as behavioral and psychological symptoms of dementia (BPSDs), including sleep disorders and mood alterations such as depression and apathy. BPSDs have a significant impact on AD patients, caregivers, public health systems, and the economy. Understanding the role of purinergic signaling, particularly the A1, A2A, and P2X7 receptors, in AD may provide new therapeutic approaches for the disease and related conditions.
Review
Biochemistry & Molecular Biology
Stephanie Franczak, Henning Ulrich, Mariusz Z. Z. Ratajczak
Summary: Hematopoiesis is regulated by various mediators, including peptide-based growth factors, cytokines, and chemokines, as well as purinergic signaling pathways and the complement cascade. Recent studies have shown that these pathways interact with specific receptors expressed on hematopoietic stem/progenitor cells (HSPC). In addition, evidence suggests that some of these receptors can be activated by intrinsically expressed ligands. Furthermore, activation of the Nox2-ROS-NLRP3 axis coordinates the responses mediated by purinergic signaling and the ComC network.
PURINERGIC SIGNALLING
(2023)
Editorial Material
Neurosciences
Qing Ye, Si-Si Lin, Henning Ulrich, Yong Tang
NEUROSCIENCE BULLETIN
(2023)
Article
Polymer Science
Emilli C. G. Frachini, Jessica S. G. Selva, Paula C. Falcoswki, Jean B. Silva, Daniel R. Cornejo, Mauro Bertotti, Henning Ulrich, Denise F. S. Petri
Summary: CAF was loaded into Gel/Alg/MNP hydrogels to achieve sustained release, and the presence of Ca2+ and EMF enhanced the release of CAF. The released concentration of CAF from Gel/Alg/MNP hydrogels in PBS/CaCl2 at 37°C was 0.35 mM, promoting cell viability.
Article
Neurosciences
Henrique Correia Campos, Deidiane Elisa Ribeiro, Debora Hashiguchi, Talita Glaser, Milena da Silva Milanis, Christiane Gimenes, Deborah Suchecki, Ricardo Mario Arida, Henning Ulrich, Beatriz Monteiro Longo
Summary: The aim of this study was to investigate the effects of resistance exercise on preventing and recovering from AD-related neuropathological conditions in APP/PS1 mice. The results showed that 4 weeks of resistance exercise improved locomotor activity and memory in APP/PS1 mice, and reduced A beta plaques and systemic inflammation. These findings suggest that resistance exercise plays a role in alleviating AD symptoms and can be an effective complementary treatment for AD.
FRONTIERS IN NEUROSCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Rebeca Perez, Viviana Burgos, Victor Marin, Antoni Camins, Jordi Olloquequi, Ivan Gonzalez-Chavarria, Henning Ulrich, Ursula Wyneke, Alejandro Luarte, Leandro Ortiz, Cristian Paz
Summary: Neurodegenerative disorders involve the progressive degeneration and death of neurons, and oxidative stress and neuroinflammation play crucial roles in their progression. Two major pathways, Nrf2 and NF-kappa B, have been identified as potential therapeutic targets due to their involvement in oxidative protection and the promotion of cytokine expression, respectively. Caffeic acid phenethyl ester (CAPE), a compound found in propolis, has shown neuroprotective activity by modulating these pathways. This review summarizes the biosynthesis and chemical synthesis of CAPE derivatives and discusses their neuroprotective activity in both in vitro and in vivo studies conducted from 2010 to 2023.
Article
Cell & Tissue Engineering
Micheli Mainardi Pillat, Ana Carolina Ayupe, Guilherme Juvenal, Carine Meinerz, Talita Glaser, Diogo Vieira da Silva Pellegrina, Daisylea Souza Paiva, Carlos Fernando Mello, Beatriz Monteiro Longo, Eduardo Moraes Reis, Henning Ulrich
Summary: Proteins involved in AD, such as APP and PS1, have a critical role in CNS development and immune response. Mutations in these proteins lead to abnormal expression in NPCs and resemble the brain pathology in adult AD patients.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Chemistry, Physical
Alex Carvalho Alavarse, Jean Bezerra Silva, Henning Ulrich, Denise Freitas Siqueira Petri
Summary: Hydrogels of poly(vinyl alcohol) (PVA)/sodium alginate (SA) with randomly distributed magnetic nanoparticles (MNPs) and magnetically oriented MNPs were prepared. Atomic force microscopy (AFM) and magnetic force microscopy (MFM) were used to evaluate the topography and magnetic domains distribution. The results showed that the orientation of magnetic domains led to an increase of surface roughness, which decreased the viability of HT-22 cells. These findings are important for situations where control of cell proliferation is necessary.
Article
Chemistry, Medicinal
Carla Fernanda Furtado Gardani, Eduardo Luiz Pedrazza, Victoria Santos Paz, Gabriele Goulart Zanirati, Jaderson Costa da Costa, Roberta Andrejew, Henning Ulrich, Juliete Nathali Scholl, Fabricio Figueiro, Liliana Rockenbach, Fernanda Bueno Morrone
Summary: This study investigated the expression of CD39 and CD73 enzymes in prostate cancer and their potential as therapeutic targets. The results showed higher levels of CD39 expression compared to CD73 in tissue samples and a correlation with Gleason score. In blood samples, CD39 expression in extracellular vesicles was significantly increased and positively correlated with ADP hydrolysis and Gleason score.
Article
Cell & Tissue Engineering
Yahaira Naaldijk, Lauren S. Sherman, Natalia Turrini, Yannick Kenfack, Mariusz Z. Ratajczak, Nizar Souayah, Pranela Rameshwar, Henning Ulrich
Summary: Neurodegenerative diseases continue to be a significant healthcare problem, and their association with neuroinflammation is well recognized. This study investigated the role of mesenchymal stem cells (MSC) in modulating activated microglial cells through exosome release. The findings suggest that MSC-induced exosomes can suppress the inflammatory response of M1 macrophages while promoting a more balanced inflammation in M2 macrophages. This insight into the potential of MSCs for tissue regeneration and neuroinflammation is valuable.
STEM CELL REVIEWS AND REPORTS
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Onesia Cristina Oliveira-Lima, Gustavo Almeida de Carvalho, Leandro Prado Assuncao, Alexandre Melo Bailao, Henning Ulrich, Antonio Carlos Pinheiro de Oliveira, Alexander Birbrair, Renato Santiago Gomez, Mauro Cunha Xavier Pinto
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)