Article
Microbiology
Jawad Alharake, Frederique Bidard, Thiziri Aouam, Catherine Senamaud-Beaufort, Antoine Margeot, Senta Heiss-Blanquet
Summary: The transcription factor RES2 in the T. reesei Rut-C30 strain contributes to higher protein secretion by adjusting the expression of genes involved in different steps of protein synthesis and the secretion pathway.
Review
Biochemistry & Molecular Biology
Muhammad Sohail, Noora Barzkar, Philippe Michaud, Saeid Tamadoni Jahromi, Olga Babich, Stanislav Sukhikh, Rakesh Das, Reza Nahavandi
Summary: Lignocellulose, a major component of plant cell walls, is a valuable feedstock for chemicals and energy production. Cellulase and xylanase are important enzymes with various industrial applications. However, little is known about the plant-cell-wall-degrading activities of yeasts.
Article
Multidisciplinary Sciences
Ewelina Golec, Alexander Ekstrom, Maciej Noga, Muhmmad Omar-Hmeadi, Per-Eric Lund, Bruno O. Villoutreix, Ulrika Krus, Katarzyna Wozniak, Olle Korsgren, Erik Renstrom, Sebastian Barg, Ben C. King, Anna M. Blom
Summary: The study finds that splice variants of CD59 exist in human and mouse pancreatic islets. These variants are associated with insulin granules and insulin secretion. In type 2 diabetes patients, the expression of these variants is significantly reduced, which may contribute to insulin deficiency.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Hongki Song, William T. Wickner, Adam Linstedt
Summary: The yeast vacuolar membrane fusion process can be achieved using a synthetic tether instead of the HOPS complex. This suggests that Sec17 and Sec18 play a crucial role in membrane fusion and may have a general role in promoting fusion across different cellular processes.
Article
Microbiology
Tyler N. Starr, Allison J. Greaney, Cameron M. Stewart, Alexandra C. Walls, William W. Hannon, David Veesler, Jesse D. Bloomid
Summary: SARS-CoV-2 virus continues to undergo mutations in the spike receptor-binding domain, affecting biochemical properties related to ACE2 receptor binding, folding stability, and antibody recognition. This study investigates the effects of these mutations in the Omicron BA.1 and BA.2 variants, showing potential changes in function compared to the ancestral strain. The research highlights the importance of ongoing surveillance and understanding the mutational landscape for effective monitoring and control of the virus.
Review
Biotechnology & Applied Microbiology
Aravind Madhavan, K. B. Arun, Raveendran Sindhu, Jayaram Krishnamoorthy, R. Reshmy, Ranjna Sirohi, Arivalagan Pugazhendi, Mukesh Kumar Awasthi, George Szakacs, Parameswaran Binod
Summary: Manufacture of recombinant therapeutics is a rapidly growing field in pharmaceuticals, with yeasts being established as important hosts for producing heterologous proteins. Advances in yeast gene manipulation tools and techniques have enabled customization of yeast cells for therapeutic protein synthesis, utilizing strategies such as secretory pathway engineering and glycosylation engineering.
MICROBIAL CELL FACTORIES
(2021)
Review
Biochemistry & Molecular Biology
Theoharis C. Theoharides, Duraisamy Kempuraj
Summary: In addition to allergic reactions, mast cells can play a critical role in homeostasis and inflammation through secretion of various mediators. The molecular mechanisms regulating this secretion, particularly the involvement of ERM proteins, are not well understood. Phosphorylation of moesin was found to be associated with the inhibition of mast cell secretion, suggesting a potential role for moesin in regulating this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Daylin Rubio-Ribeaux, Rogger Alessandro Mata da Costa, Renan Murbach Pereira, Paulo Ricardo Franco Marcelino, Fernanda Perpetua Casciatori, Julio Cesar dos Santos, Silvio Silverio da Silva
Summary: This study investigated the impact of LED light on bioemulsifier production by Aureobasidium pullulans LB83 in solid-state fermentation. The results showed that blue light significantly enhanced the production of bioemulsifier and cellulolytic enzyme activity. The bioreactor with blue LED light outperformed others in terms of bioemulsifier production.
FERMENTATION-BASEL
(2023)
Review
Food Science & Technology
Eleni Kamilari, Catherine Stanton, F. Jerry Reen, R. Paul Ross
Summary: This review highlights the biotechnological importance of the filamentous yeast-like fungus Geotrichum candidum, which is widely used as a starter in the dairy industry. The study found that aside from its role in the dairy and brewing industries, G. candidum has potential as a probiotic nutritional supplement in fish. Additionally, this species produces various biotechnologically important enzymes, antimicrobial compounds, and has bioremediation capabilities.
Article
Chemistry, Multidisciplinary
Mette Galsgaard Malle, Philipp M. G. Loeffler, Soren S-R Bohr, Magnus Berg Sletfjerding, Nikolaj Alexander Risgaard, Simon Bo Jensen, Min Zhang, Per Hedegard, Stefan Vogel, Nikos S. Hatzakis
Summary: In this study, a single-particle combinatorial multiplexed liposome fusion mediated by DNA is presented, enabling multistep and non-deterministic fusion of nanocontainers. Efficient and leakage-free stochastic fusion sequences were observed for arrays of target liposomes functionalized with individual DNA strands and labeled with distinct chromophores. The method allows high-throughput combinatorial multiplex screens using minimal material.
Article
Chemistry, Multidisciplinary
Ao Xia, Kai Lin, Tong Zhu, Yun Huang, Xianqing Zhu, Xun Zhu, Kaiyong Cai, Zidong Wei, Qiang Liao
Summary: Inspired by termite digestion, this study proposed a two-stage microreactor system loaded with enzymes for efficient hydrolysis of wheat straw. The results showed that the continuous action of cellulase and xylanase in this system significantly improved the enzymatic hydrolysis efficiency of lignocellulosic biomass and increased glucose production.
Review
Biochemistry & Molecular Biology
Azzurra Margiotta
Summary: The myelin sheath is an important insulating layer around nerves that allows fast and efficient nerve conduction. It is formed by different cells in the central and peripheral nervous system. The myelin sheath's structure and composition play a crucial role in maintaining proper nerve function. The proteins SNAREs and rabs have been found to be involved in regulating myelin formation and maintenance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maria Gorczyca, Jan Kazmierczak, Patrick Fickers, Ewelina Celinska
Summary: Overproduction of recombinant secretory proteins triggers changes in producer cell physiology, and exposure to suboptimal growth conditions may increase this response. Studying the effects of different environmental stresses on protein synthesis and host cell physiology can improve understanding of fundamental biological processes and provide clues for optimizing bioprocesses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anica Dadwal, Shilpa Sharma, Tulasi Satyanarayana
Summary: Cellulases play a crucial role in the bioconversion of renewable lignocellulosic biomass, but low production titres lead to high enzyme costs, affecting the success of ethanol-based biorefineries. Stable cellulases are important for their wider application in industrial processes active at acidic/alkaline pH ranges, organic solvents, and salt tolerance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Akira Hosomi, Chinatsu Okachi, Yudai Fujiwara
Summary: Soluble proteins with a signal peptide are translocated into the endoplasmic reticulum (ER), while some proteins without a signal peptide, like SOD1, can still enter the ER. SOD1 is a causative gene of ALS, and its secretion has been implicated in the pathogenesis of ALS. This study reveals that SOD1 is translocated into the ER lumen through the translocon Sec61 and subsequently secreted extracellularly, suggesting the potential of targeting SOD1 secretion as a therapeutic approach for ALS.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Gillian Cedras, Heinrich Kroukamp, Willem Heber Van Zyl, Riaan Den Haan
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Jie Li, Ming-Ming Zhang, Chun Wan, Riaan Den Haan, Feng-Wu Bai, Xin-Qing Zhao
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Kentaro Inokuma, Hiroki Kurono, Riaan den Haan, Willem Heber van Zyl, Tomohisa Hasunuma, Akihiko Kondo
METABOLIC ENGINEERING
(2020)
Review
Biotechnology & Applied Microbiology
S. A. Davison, R. den Haan, W. H. van Zyl
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Chemistry, Physical
Samkelo Malgas, Shaunita H. Rose, Willem H. van Zyl, Brett Pletschke
Article
Biotechnology & Applied Microbiology
Xue Zhang, Liang Wang, Qian Li, Riaan den Haan, Fan Li, Chen-Guang Liu, Feng-Wu Bai
Summary: This study characterized the oscillatory behavior of yeast cells during continuous very-high-gravity ethanol fermentation, revealing that glucose transport and phosphorylation could be key nodes for regulating intracellular metabolism, and stress response genes were predominantly triggered and affected by ethanol inhibition in yeast cells.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
B. A. Brandt, T. Jansen, H. Volschenk, J. F. Gorgens, W. H. Van Zyl, R. Den Haan
Summary: The 2G fermentation environment for lignocellulose conversion presents unique challenges to fermentative organisms, including inhibitor stress, enzyme product tolerance stress, and other stresses. By utilizing rational engineering, reverse engineering, and adaptation strategies, identifying genes or alleles that contribute positively to the overall robustness of 2G industrial strains is essential.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kentaro Inokuma, Yuki Kitada, Takahiro Bamba, Yuma Kobayashi, Takahiro Yukawa, Riaan den Haan, Willem Heber van Zyl, Akihiko Kondo, Tomohisa Hasunuma
Summary: The knockout of CCW12 and CCW14 genes has a significant impact on the carrying capacity of heterologous proteins in yeast cell walls, with the co-knockout strains showing increased protein loading capacity. This was accompanied by an increase in cell wall thickness in the co-knockout strains, indicating a potential enhancement in protein carrying capacity. The CCW12 and CCW14 co-knockout strains are therefore promising hosts for constructing highly functional recombinant yeast strains using cell-surface display technology.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Chemistry, Physical
Sesethu Gift Njokweni, Annica Steyn, Marelize Botes, Marinda Viljoen-Bloom, Willem Heber van Zyl
Summary: The implementation of a biobased economy in developing countries like South Africa requires the utilization of sustainable feedstock technologies to produce valuable bio-based products. By utilizing different parts of biomass waste streams, high-value products can be produced.
Review
Biotechnology & Applied Microbiology
Riaan den Haan, Shaunita H. Rose, Rosemary A. Cripwell, Kim M. Trollope, Marthinus W. Myburgh, Marinda Viljoen-Bloom, Willem H. van Zyl
Summary: This review discusses the use of consolidated bioprocessing to address the high cost of exogenous enzymes in commercial bioethanol production, as well as strategies and lessons learned for improving recombinant protein production in Saccharomyces cerevisiae over the past forty years. Host strain selection and modification, as well as enzyme compatibility, are found to be crucial determinants for success.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biotechnology & Applied Microbiology
Francois Kruger, Riaan den Haan
Summary: The study successfully integrated and expressed xylanolytic enzyme-encoding genes. Secretion resulted in higher overall xylosidase activity when strains were cultivated on glucose, while cell-associated activity was higher when cultivated in the presence of xylose. The combination of both enzymes and cell-associated xylosidase production led to the best growth on both xylan and XOS substrates.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Bronwyn Jean Chetty, Kentaro Inokuma, Tomohisa Hasunuma, Willem Heber van Zyl, Riaan den Haan
Summary: Enhanced cellulase activity on the cell surface of engineered strains for CBP was achieved through overexpression of cellulase secretion genes. The improved strains showed significant increases in cellulolytic activity and ethanol yields, making them more efficient for 2G biofuel production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Odwa Jacob, Gert Rutger van Lill, Riaan den Haan
Summary: This study tested the integration of cellulase encoding genes into various sites on the yeast genome to inform the best strategy for creating cellulolytic strains for consolidated bioprocessing. Targeting specific genes to specific loci had dramatic effects on strain efficiency, highlighting the importance of careful genetic engineering in optimizing strains for lignocellulose conversion.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Letitia Minnaar, Riaan den Haan
Summary: This study evaluates the potential of natural and industrial strains of Saccharomyces cerevisiae to secrete cellulases. The results show that natural isolates have better secretion capacities for cellulases under high temperature and acetic acid conditions, and exhibit tolerance to various fermentation-associated stresses. These isolates could serve as promising chassis organisms for consolidated bioprocessing bioethanol production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Steffi A. Davison, Riaan den Haan, Willem Heber van Zyl
FEMS YEAST RESEARCH
(2019)