Article
Chemistry, Analytical
Ivan Vuckovic, Aleksandar Denic, M. Cristine Charlesworth, Milovan Suvakov, Shane Bobart, John C. Lieske, Fernando C. Fervenza, Slobodan Macura
Summary: Several postprocessing methods for measuring protein concentrations in human urine using existing H-1 nuclear magnetic resonance (NMR) metabolomic spectra were described, including direct spectral integration, integration of different spectra, and matching protein patterns. The study found excellent correlation between NMR integrals and protein content measured by the BCA assay, and indicated that TSP spectral parameters can be good indicators of protein concentration.
ANALYTICAL CHEMISTRY
(2021)
Article
Cardiac & Cardiovascular Systems
Chiara Bruzzone, Ruben Gil-Redondo, Marisa Seco, Rocio Barragan, Laura de la Cruz, Claire Cannet, Hartmut Schaefer, Fang Fang, Tammo Diercks, Maider Bizkarguenaga, Beatriz Gonzalez-Valle, Ana Lain, Arantza Sanz-Parra, Oscar Coltell, Ander Lopez de Letona, Manfred Spraul, Shelly C. Lu, Elisabetta Buguianesi, Nieves Embade, Quentin M. Anstee, Dolores Corella, Jose M. Mato, Oscar Millet
Summary: Metabolic syndrome (MetS) is a complex condition without a clear medical definition. This study used NMR metabolomics to analyze urine samples from a European cohort, revealing a unique molecular signature of MetS. The results showed that MetS progression is associated with specific changes in the urine metabolome, which can be used to identify individuals with MetS and provide insight into the transitional states between healthy individuals and MetS patients.
CARDIOVASCULAR DIABETOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Victor Ribay, Arnab Dey, Benoit Charrier, Clement Praud, Joris Mandral, Jean-Nicolas Dumez, Marine P. M. Letertre, Patrick Giraudeau
Summary: Hyperpolarized nuclear magnetic resonance (NMR) methods, particularly Dissolution Dynamic Nuclear Polarization (d-DNP), greatly enhance the sensitivity of detecting C-13 NMR signals. This study demonstrates the first application of d-DNP-enhanced C-13 NMR analysis of biofluid (urine) at natural abundance, providing unprecedented resolution and sensitivity. Accurate quantitative information on multiple targeted metabolites can be obtained through a standard addition procedure.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Ivica Kopriva, Ivanka Jeric, Marijana Popovic Hadzija, Mirko Hadzija, Marijana Vucic Lovrencic
Summary: The proposed method introduces a library-based approach to quantify proportions of overlapping metabolites from H-1 NMR mixture spectra, addressing the challenge of metabolite concentration analysis. By utilizing the linear non-negative least squares (NNLS) problem, the method successfully made progress in differentiating metabolites that discriminate the T2DM group from the control group.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Panagiota D. Georgiopoulou, Styliani A. Chasapi, Irene Christopoulou, Anastasia Varvarigou, Georgios A. Spyroulias
Summary: Neonatal sepsis is a critical medical condition that occurs after preterm birth, and it is challenging to accurately diagnose or predict the disease. This study aimed to investigate the urine metabolomic profile of septic neonates through nuclear magnetic resonance (NMR) and identify potential biomarkers for distinguishing between healthy and diseased newborns.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Vasiliki Falaina, Charalambos Fotakis, Theodora Boutsikou, Thalia Tsiaka, Georgios Moros, Sotirios Ouzounis, Vasiliki Andreou, Zoi Iliodromiti, Theodoros Xanthos, Yvan Vandenplas, Nicoletta Iacovidou, Panagiotis Zoumpoulakis
Summary: This study compared the urine metabolic fingerprint of breastfed and synbiotic formula-fed neonates. No distinct differences were observed in the first two time points, but discrimination trends were observed after the 2nd month.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Zachary R. Wanner, Cormac G. Southam, Prachi Sanghavi, Naveenjyote S. Boora, Eric J. Paxman, Sean P. Dukelow, Brian W. Benson, Tony Montina, Gerlinde A. S. Metz, Chantel T. Debert
Summary: This study examined the metabolic changes post-sport-related concussion (SRC) through urine analysis, identifying specific metabolites associated with SRC and suggesting a more objective method for characterizing the condition. The correlations found between specific metabolites and factors like symptom burden and length of return an potential applications as biomarkers for SRC diagnosis and treatment.
FRONTIERS IN NEUROLOGY
(2021)
Article
Cell Biology
Cristina Solana-Manrique, Francisco Jose Sanz, Isabel Torregrosa, Martina Palomino-Schatzlein, Carolina Hernandez-Oliver, Antonio Pineda-Lucena, Nuria Paricio
Summary: The study investigates metabolic disturbances underlying the pathogenesis of Parkinson's disease (PD) using a Drosophila PD model. Through metabolomic analysis, the study finds that metabolic alterations are associated with PD and could potentially serve as therapeutic targets and biomarkers for the disease.
Review
Food Science & Technology
Valentina Maestrello, Pavel Solovyev, Luana Bontempo, Luisa Mannina, Federica Camin
Summary: Extra virgin olive oil (EVOO) is a high-quality product that has become a target of food fraud in recent years. The use of nuclear magnetic resonance (NMR) spectroscopy is reviewed as a reliable and rapid tool to authenticate EVOOs and detect adulteration and mislabeling.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Fisheries
Hua Yang, Xueni Qiu, Yun Zhao, Yangfang Ye, Weiwei Song, Changkao Mu, Ronghua Li, Chunlin Wang
Summary: This study investigated the optimal anesthetic concentration for cuttlefish using NMR and HPLC analysis. They found that ethanol at 10-30 mL L-1 successfully anesthetized cuttlefish, with recovery observed after 60 minutes at 10 mL L-1 concentration. Cuttlefish also reached a sedative state below 5 mL L-1 ethanol, with a maximum exposure time of 270 minutes. The action of ethanol led to changes in neurotransmitter levels, with increased inhibitory neurotransmitters and decreased excitatory neurotransmitters observed.
Review
Biochemistry & Molecular Biology
Jesus Brezmes, Maria Llambrich, Raquel Cumeras, Josep Guma
Summary: Metabolomics is a crucial method for discovering novel biomarkers and their application in precision medicine. NMR-based metabolomics in urine can serve as a powerful clinical tool in precision oncology. This review provides a comprehensive evaluation of NMR metabolomics for the detection of colorectal cancer (CRC) in urine, identifying potential relevant compounds and metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maxim Fomenko, Lyudmila Yanshole, Yuri P. Tsentalovich
Summary: Thermal and enzymatic reactions can significantly alter tissue metabolomic content during sample preparation. Stability of metabolites in human blood, serum, rat brain, and their extracts was evaluated. The results demonstrated that metabolites in tissue extracts remained stable for 24 hours at 4 degrees C. Serum and whole blood metabolomes were also relatively stable, with noticeable changes in whole blood only occurring after 1-2 hours at 4 degrees C, and becoming more significant after 24 hours. The most significant changes were observed in energy metabolites, with a decrease in ATP and ADP concentrations and below detectable levels for NAD, NADH, and NADPH. AMP, IMP, hypoxanthine, and nicotinamide showed a statistically significant increase. Brain tissue exhibited more rapid metabolomic changes during harvest and homogenization, with noticeable changes in all compound classes observed over longer time periods.
Article
Biochemistry & Molecular Biology
Kerti Ausmees, Nele Reimets, Indrek Reile
Summary: Parahydrogen hyperpolarization is a promising tool for sensitively enhancing NMR metabolomics, allowing for resolution and quantification of low-abundance metabolites. The reliable identification of analytes producing parahydrogen hyperpolarized NMR signals currently requires the addition of internal standards, which can be laborious. This study demonstrates that generating libraries of hyperpolarized analyte signals, similar to regular NMR metabolomics, can address this limitation.
Article
Neurosciences
Marina Botello-Marabotto, M. Carmen Martinez-Bisbal, Miguel Calero, Andrea Bernardos, Ana B. Pastor, Miguel Medina, Ramon Martinez-Manez
Summary: This study employed 1H NMR spectroscopy to conduct a metabolomic study in serum samples from patients with Alzheimer's disease (AD), mild cognitive impairment (MCI), and cognitively healthy controls (HC). The aim was to search for potential metabolite biomarkers. The results highlight the potential of 1H NMR metabolomics to support the diagnosis of dementia in a less invasive way, and provide a starting point for the study of potential biomarkers to identify MCI or HC subjects at risk of developing AD in the future.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Oncology
Annabel Steiner, Stefan Andreas Schmidt, Cara Sophie Fellmann, Johannes Nowak, Chin-Lee Wu, Adam Scott Feldman, Meinrad Beer, Leo L. Cheng
Summary: Prostate cancer is the second most diagnosed cancer in men. Current diagnostic standards lack reliability in detecting and characterizing prostate cancer. This study found that metabolomic information can differentiate between benign and malignant prostate tissue, and also predict future prostate cancer diagnosis, making it helpful for early detection.
Article
Environmental Sciences
Celia Alves, Margarita Evtyugina, Estela Vicente, Ana Vicente, Ismael Casotti Rienda, Ana Sanchez de la Campa, Mario Tome, Iola Duarte
Summary: This study collected PM2.5 samples near an industrial chemical pole and analyzed their OC, EC, trace elements, and organic constituents. The results showed that organic compounds, elemental carbon, and trace elements mainly originated from industrial emissions, nearby roads, and biomass burning. Some organic constituents were likely related to plasticizer ingredients and thermal stabilizers in industrial emissions. Additionally, principal component analysis revealed the contributions of different emission sources.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Mariana R. Silva, Gualter S. Silva, Sara Fernandes, Joao Almeida, Paulo Fonseca, Marco Oliveira, Helena Goncalves, Francisca Saraiva, Antonio S. Barros, Pedro G. Teixeira, Ricardo L. Lopes, Francisco Sampaio, Silvia O. Diaz, Joao Primo, Ricardo Fontes-Carvalho
Summary: This study aimed to determine the predictors of early recurrence (ER) and late recurrence (LR) after catheter ablation (CA) for atrial fibrillation (AF) and establish the risk of LR in patients who experience ER. The results showed that the risk of LR after CA was significantly higher in patients with ER (five-fold increased risk). These findings highlight the importance of clarifying the clinical role of the blanking period.
JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
(2023)
Article
Food Science & Technology
Evla D. F. Vieira, David Styles, Sergio Sousa, Carla Santos, Ana M. Gil, Ana M. Gomes, Marta W. Vasconcelos
Summary: Studies show that adding pulses to foods increases their nutritional value. This study focuses on lupin flour's effects on yogurt, finding that lupin-enriched yogurt is high in protein, fiber, and omega 3. Rheological tests also indicate that this yogurt is stiffer and more viscous. Sensory analysis shows no significant differences compared to controls, but bitterness, granularity, and after-taste are observed. Using lupin flour to enhance yogurt production could be an alternative for creating more nutritious products.
INTERNATIONAL JOURNAL OF GASTRONOMY AND FOOD SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Carlos Iglesias Pastrana, Juan Vicente Delgado Bermejo, Maria Noemi Sgobba, Francisco Javier Navas Gonzalez, Lorenzo Guerra, Diana C. G. A. Pinto, Ana M. Gil, Iola F. Duarte, Giovanni Lentini, Elena Ciani
Summary: Studies on the therapeutic properties and metabolomic profile of camel urine are limited and lack standardization. Standardization and interdisciplinary approaches are needed to improve the interpretation and replicability of results. Sample enlargement can help evaluate intra- and interindividual variability and distinguish factors that may affect the composition and bioactivity of camel urine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tatiana J. Carneiro, Martin Vojtek, Salome Goncalves-Monteiro, Ana L. M. Batista de Carvalho, Maria Paula M. Marques, Carmen Diniz, Ana M. Gil
Summary: This study used Nuclear Magnetic Resonance metabolomics to compare the effects of Pd(2)Spermine and cisplatin on the brain and liver. The results showed that Pd(2)Spermine had a stronger impact on the brain metabolome compared to cisplatin. Both drugs did not cause neuronal damage or inflammation. Pd(2)Spermine seemed to enhance brain anti-inflammatory and antioxidant mechanisms, while cisplatin caused more liver damage and increased the need for liver regeneration processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Sara Lopes Fernandes, Ricardo Ladeiras-Lopes, Mariana Silva, Gualter Silva, Ines Cruz, Silvia O. Diaz, Antonio S. Barros, Francisca Saraiva, Rita Faria, Joao Almeida, Paulo Fonseca, Helena Goncalves, Marco Oliveira, Nuno Ferreira, Joao Primo, Ricardo Fontes-Carvalho
Summary: Coronary artery calcium score (CACS) is associated with an increased risk of atrial fibrillation (AF) recurrence, particularly when the score exceeds 100.
JOURNAL OF CARDIOVASCULAR COMPUTED TOMOGRAPHY
(2023)
Article
Cell Biology
Daniela S. C. Bispo, Catarina S. H. Jesus, Katarzyna Romek, Ines M. C. Marques, Mariana B. Oliveira, Joao F. Mano, Ana M. Gil
Summary: This paper presents an untargeted NMR metabolomics study on the proliferation and osteogenic differentiation of human adipose mesenchymal stem cells (hAMSCs), aiming to identify potential metabolic markers that are dependent on or independent of cell donors. By analyzing the polar endometabolome of hAMSCs from two donors with distinct characteristics, the study suggests an 18-metabolites signature for cell proliferation and a set of 11 metabolites for osteogenesis. These signatures have been validated in a third donor and may contribute to monitoring and predicting the proliferation and osteogenic ability of different donors.
Article
Cardiac & Cardiovascular Systems
Ines Cruz, Sara Lopes Fernandes, Silvia O. Diaz, Francisca Saraiva, Antonio S. Barros, Joao Primo, Francisco Sampaio, Ricardo Ladeiras-Lopes, Ricardo Fontes-Carvalho
Summary: There is no independent association between epicardial adipose tissue (EAT) volume and attenuation and the risk of atrial fibrillation (AF) recurrence after AF ablation.
REVISTA ESPANOLA DE CARDIOLOGIA
(2023)
Article
Biochemistry & Molecular Biology
Maite Garcia-Guasch, Eduard Escrich, Raquel Moral, Iola F. Duarte
Summary: The aim of this study was to investigate the effects of two phenolic compounds, hydroxytyrosol (HT) and luteolin (LUT), on the metabolism of breast cancer cells of different molecular subtypes. The researchers used untargeted metabolomics to study the metabolic responses of MDA-MB-231 and MCF-7 cells to treatment with these phenols. The results showed that the effects of the phenolic compounds varied depending on the cell type and included changes in mitochondrial metabolism, glycolysis, lipid metabolism, and cellular redox status.
Article
Oncology
Ana S. Dias, Catarina R. Almeida, Luisa A. Helguero, Iola F. Duarte
Summary: Tumor-associated macrophages (TAMs) are the most abundant immune cells in the breast tumor microenvironment, and their immune-suppressive functions promote breast cancer progression. The metabolic crosstalk between tumor cells and TAMs is important in modulating the tumor microenvironment towards anti-tumor immunity. This study investigates how tumor-educated macrophages (TEM) reprogram their metabolism and phenotype in response to different metabolic conditions imposed by breast cancer cell subtypes, providing new insights into the metabolic plasticity of TEM in the breast cancer microenvironment.
Article
Chemistry, Multidisciplinary
Maria V. Monteiro, Margarida Henriques-Pereira, Bruno M. Neves, Iola D. Duarte, Vitor M. Gaspar, Joao F. Mano
Summary: Tissue-derived decellularized extracellular matrix (dECM) combined with hyaluronic acid (HA) and tyramine can create highly physiomimetic tumor models for studying tumor-stroma interaction, which can be used for screening pancreatic cancer therapies.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cardiac & Cardiovascular Systems
Ana Filipa Ferreira, Maria Joao Azevedo, Juliana Morais, Fabio Trindade, Francisca Saraiva, Silvia Oliveira Diaz, Ines Nuno Alves, Mariana Fragao-Marques, Carla Sousa, Ana Paula Machado, Adelino Leite-Moreira, Benedita Sampaio-Maia, Carla Ramalho, Antonio Sousa Barros, Ines Falcao-Marques
Summary: Pregnant women with cardiovascular risk factors are at high risk of developing cardiovascular disease later in life. Our study found that pregnant women undergo cardiovascular remodeling during pregnancy, which reverses after delivery. Arterial hypertension, smoking, and obesity are independent predictors of increased left ventricular mass. Additionally, circulating biomarkers such as C-reactive protein and ST2/IL33 receptors may be associated with worse cardiac remodeling.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rita Nogueira-Ferreira, Ines Santos, Rita Ferreira, Dulce Fontoura, Claudia Sousa-Mendes, Ines Falcao-Pires, Andre P. Lourenco, Adelino Leite-Moreira, Iola F. Duarte, Daniel Moreira-Goncalves
Summary: Exercise training has positive effects on the management of metabolic syndrome, improving inflammation, exercise capacity, and muscle metabolism. It reduces muscle atrophy and fibrosis and enhances oxidative metabolism, reducing fatigue susceptibility.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Joao A. Rodrigues, Daniela S. C. Bispo, Monica G. Silva, Rita Araujo, Amadeu M. V. M. Soares, Rosa Freitas, Ana M. Gil
Summary: This study investigates the effects of exposure to 17-α-ethinylestradiol (EE2) on lipophilic extracts of Ruditapes philippinarum clams at 17 degrees C and 21 degrees C. The results show that EE2 exposure at 17 degrees C leads to a slight increase in membrane rigidity at low concentrations, while at higher concentrations, lipid metabolism stabilizes. In contrast, at 21 degrees C, lipid metabolism responds to EE2 at 125 ng/L, with the antioxidant docosahexaenoic acid (DHA) helping to mitigate oxidative stress. The study also identifies new potential markers of stress mitigation, such as high levels of phosphatidylcholine (PtdCho), polyunsaturated fatty acids (PUFAs), and linoleic acid, as well as low PUFA/glycine ratios.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Rita Araujo, Victoria Fabris, Caroline A. Lamb, Andres Elia, Claudia Lanari, Luisa A. Helguero, Ana M. Gil
Summary: The lipid metabolism adaptations of estrogen and progesteronereceptor-positive breast cancer tumors in relation to HD to HI tumor growth transition and HIR tumor acquisition of ET resistance were investigated. Results showed increased nonesterified cholesterol forms and phospholipids, and decreased triglycerides and fatty acids during tumor progression. These changes suggest a shift toward more activated cholesterol synthesis, β-oxidation, and phospholipid biosynthesis to sustain tumor growth. Certain compound levels and ratios were identified as potential markers for HD-HI-HIR progression.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
