Article
Biochemistry & Molecular Biology
Szabolcs Dvoracsko, Laszlo Lazar, Ferenc Fulop, Marta Palko, Zita Zalan, Botond Penke, Livia Fulop, Csaba Tomboly, Ferenc Bogar
Summary: A new ensemble docking-based virtual screening protocol utilizing both agonist and antagonist bound S1R structures was developed in this study, leading to the identification of three novel high affinity S1R ligands, one of which exhibited notable S1R/S2R selectivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Amer H. Tarawneh, Pankaj Pandey, Lo'ay A. Al-Momani, Anastassiya Gadetskaya, Sultan T. Abu-Orabi, Robert J. Doerksen, Stephen J. Cutler
Summary: The CB2 receptor, as a potential target for treating various neuroinflammatory or neurodegenerative diseases, has attracted significant interest, and the search for new compounds as highly selective CB2 modulators is of great importance.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Anna Vlachodimou, Henk de Vries, Milena Pasoli, Miranda Goudswaard, Soon-Ai Kim, Yong-Chul Kim, Mirko Scortichini, Melissa Marshall, Joel Linden, Laura H. Heitman, Kenneth A. Jacobson, Adriaan P. IJzerman
Summary: A(2B) adenosine receptor (A(2B)AR) antagonists have therapeutic potential in inflammation-related diseases, but there is a need for novel antagonists. This study investigates the synthesis and biological evaluation of xanthine derivatives as A(2B)AR antagonists. The study finds a clear relationship between a ligand's A(2B)AR residence time and its functional effect, highlighting the importance of binding kinetics in early drug discovery.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Xiaojun Lyu, Vahid Hamedpour, Yui Sasaki, Zhoujie Zhang, Tsuyoshi Minami
Summary: A paper-based chemosensor array device (PCSAD) was proposed for simultaneous classification of 12 saccharides and quantification of fructose and glucose. The mechanism relied on indicator displacement assay (IDA) using catechol dyes, 3-nitrophenylboronic acid, and saccharides for qualitative, semiquantitative, and quantitative analyses. The chemosensors showed clear color changes for discrimination of saccharides and detection of fructose in commercial soft drinks.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Emma L. Farquharson, Ashlyn Lightbown, Elsi Pulkkinen, Tea Russell, Brenda Werner, Sam R. Nugen
Summary: Phages have potential in bacterial disease control and diagnostics, but current definitions of their host range are limited. A new filtration-based bacterial binding assay has been developed to evaluate phages' adsorptive capabilities.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Seiya Asai, Lenka Zakova, Irena Selicharova, Ales Marek, Jiri Jiracek
Summary: The study utilized an IR binding assay to determine insulin levels, showing comparable results to RIA kits. Arginine stimulated glucose-induced insulin secretion in MIN6 cells, while dopamine had inhibitory effects. The effects of serotonin varied with concentration levels. The IR binding assay allows for higher throughput and broader range of measurements compared to RIA kits.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Veterinary Sciences
Caroline Schoeller, Sandra Hoffmann, Stephanie Adolph, Ralf Regenthal, Getu Abraham
Summary: This study characterized the specific binding sites for [N-methyl-3H]-scopolamine in membranes of turkey heart chambers, revealing the presence of M2 and M3 receptor subtypes, with M2 being predominant. There were differences in receptor density between BUT Big 6 and Cro center dot llwitzer turkeys in heart chambers.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Mona Khoramjouy, Fatemeh Ahmadi, Mehrdad Faizi, Soraya Shahhosseini
Summary: This study applied a centrifugation method to separate free and bound radioligands, instead of using a filtration method, and optimized conditions for radioligand receptor binding studies of mu-opioid receptors, saturation, and competition. The results showed repeatable and reliable outcomes, demonstrating the effectiveness of the centrifugation method in screening new analogs of mu-opioid receptors.
PHARMACOLOGICAL REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Zsuzsanna Szabo, Balazs Dezso, Klara Fodor, Krisztian Szegedi, Tibor Flasko, Erzsebet Szabo, Gabor Olah, Eva Sipos, Nikoletta Dobos, Janos Gardi, Andrew V. Schally, Gabor Halmos
Summary: This study investigates the expression of LHRH and LHRH-R-I in transitional cell carcinoma (TCC) type of human bladder cancer, finding high incidence of expression of both in the samples examined. Positive immunostaining for LHRH-R-I with different expression intensity was found in all samples, showing a negative correlation with TCC grade.
Article
Pharmacology & Pharmacy
Qianwa Liang, Sumit Joshi, Hui Liu, Yanbo Yu, Haiyang Zhao, Tammie L. S. Benzinger, Joel S. Perlmutter, Zhude Tu
Summary: The vesicular acetylcholine transporter (VAChT) plays a crucial role in the cholinergic system and alterations in it are associated with neurodegenerative disorders. A PET imaging tracer, [F-18]VAT, was recently developed to target VAChT in vivo with high affinity and selectivity. In vitro characterization of the tritiated counterpart of [F-18]VAT, [H-3]VAT, showed a saturated binding curve with VAChT-rich cell membrane extracts and binding inhibition by VAChT ligands, supporting [F-18]VAT as a suitable PET radiotracer for quantitatively assessing VAChT levels in vivo.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dimitrios Baltzis, George Z. Tsogas, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: A high-throughput fluorimetric assay for histidine was developed using a 96-well plates platform. The method detects histidine in human urine samples using a smartphone after illumination under UV light without the need for instruments. The results were in good agreement with an HPLC corroborative method, and the assay showed minimal interference from other substances in urine.
Article
Pharmacology & Pharmacy
Julie V. Selkirk, Andrea Bortolato, Yingzhuo Grace Yan, Nathan Ching, Richard Hargreaves
Summary: This study investigates the binding of multiple S1P receptor modulators to S1P(1) and S1P(5), and finds that they can all be considered interchangeable. Therefore, the choice of medication should be based on overall therapeutic profile, without concern for additive S1P pharmacology.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Ciara Frances Pugh, Brian Thomas DeVree, Solveig Gaarde Schmidt, Claus Juul Loland
Summary: This study functionally characterized purified, full length dDAT and investigated the effects of Na+ and Cl- binding on its affinity. The pharmacological profile of purified, full length dDAT was established through comparison with other systems, providing important insights for subsequent biophysical studies.
Article
Environmental Sciences
Elijah J. Petersen, Richard Uhl, Blaza Toman, John T. Elliott, Judy Strickland, James Truax, John Gordon
Summary: The Electrophilic Allergen Screening Assay (EASA) is a promising method for detecting the first key event in the adverse outcome pathway for skin sensitization. This study describes the redesign of EASA into a 96-well plate format that incorporates in-process control measurements to increase confidence in the assay results.
Article
Chemistry, Analytical
Adam Szmelter, Jason Jacob, David T. Eddington
Summary: Researchers have developed a device that can deliver 12 unique oxygen concentrations to cells in a 96-well plate, allowing for in-depth studies on the effects of varying oxygen concentrations on cell responses. This technology seamlessly integrates into biomedical research workflows, demonstrating the potential for increased throughput in oxygen studies.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Organic
Sreedhar Reddy Tummalapalli, Rohit Bhat, Craig Waitt, Henk Eshuis, David P. Rotella
TETRAHEDRON LETTERS
(2017)
Article
Multidisciplinary Sciences
Andrew M. Tobias, Dea Toska, Keith Lange, Tyler Eck, Rohit Bhat, Cheryl A. Janson, David P. Rotella, Ueli Gubler, Nina M. Goodey
Article
Chemistry, Medicinal
Sreedhar R. Tummalapalli, Rohit Bhat, Agnieszka Chojnowski, Monika Prorok, Tamara Kreissir, Ronald Goldberg, Stacie Canan, Natalie Hawryluk, Deborah Mortensen, Vikram Khetan, Jerome Zeldis, John J. Siekierka, David P. Rotella
ACS MEDICINAL CHEMISTRY LETTERS
(2018)
Article
Chemistry, Medicinal
Rohit Bhat, James A. Fishback, Rae R. Matsumoto, Jacques H. Poupaert, Christopher R. McCurdy
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2013)
Article
Chemistry, Medicinal
Rohit Bhat, Amna T. Adam, Jungeun Jasmine Lee, Gaspard Deloison, Yves Rouille, Karin Seron, David P. Rotella
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2014)
Article
Chemistry, Medicinal
Daniel J. Buzard, Sun Hee Kim, Juerg Lehmann, Sangdon Han, Imelda Calderon, Amy Wong, Andrew Kawasaki, Sanju Narayanan, Rohit Bhat, Tawfik Gharbaoui, Luis Lopez, Dawei Yue, Kevin Whelan, Hussien Al-Shamma, David J. Unett, Hsin-Hui Shu, Shiu-Feng Tung, Steve Chang, Ching-Fen Chuang, Michael Morgan, Abu Sadeque, Zhi-Liang Chu, James N. Leonard, Robert M. Jones
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2014)
Article
Chemistry, Medicinal
Rohit Bhat, Amna T. Adam, Jungeun Jasmine Lee, Thomas A. Gasiewicz, Ellen C. Henry, David P. Rotella
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2014)
Review
Chemistry, Medicinal
Sanju Narayanan, Rohit Bhat, Christophe Mesangeau, Jacques H. Poupaert, Christopher R. McCurdy
FUTURE MEDICINAL CHEMISTRY
(2011)
Article
Chemistry, Medicinal
Rohit Bhat, Sreedhar R. Tummalapalli, David P. Rotella
JOURNAL OF MEDICINAL CHEMISTRY
(2014)
Article
Medicine, General & Internal
Suvarna Patil, Netaji Patil, Charudatta Joglekar, Arvind Yadav, Anup Nilawar, Ulka Banavali, Rohit Bhat, Vijay Dombale, Bhushan Warpe, Rachana Mohite, Kiran Joshi
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)