期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 66, 期 -, 页码 170-175出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2012.03.041
关键词
Citrus medica var. sarcodactylis; Coumarin; Flavonoid; HPLC-DAD; Limonoid
资金
- Macau Science and Technology Development Fund [013/2008/A1, SKL/QRCM/EST/11-Y1/ICMS]
- Natural Science Foundation of Guangdong [8351063201000003]
- Jiangsu Province Academy of Chinese Medicine [RC1101, 2011NDDCM02003]
A high performance liquid chromatography coupled with diode array detector (HPLC-DAD) method was developed for simultaneous quantification of eleven major bioactive components including six coumarins, three flavonoids and two limonoids in Fructus Citri Sarcodactylis. The analysis was performed on a Cosmosil 5 C-18-MS-II column (4.6 mm x 250 mm, 5 mu m) with water-acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability and accuracy. It was found that the calibration curves for all analytes showed good linearity (R-2 > 0.9993) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) were less than 3.0 and 10.2 ng. The relative standard deviations (RSDs) for intra- and inter-day repeatability were not more than 4.99% and 4.92%, respectively. The sample was stable for at least 48 h. The spike recoveries of eleven components were 95.1-104.9%. The established method was successfully applied to determine eleven components in three samples from different locations. The results showed that the newly developed HPLC-DAD method was linear, sensitive, precise and accurate, and could be used for quality control of Fructus Citri Sarcodactylis. (C) 2012 Elsevier B.V. All rights reserved.
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