Article
Biochemical Research Methods
Tengfei Yuan
Summary: In this study, a sample-multiplexing LC-MS/MS method was developed and validated for the quantification of serum estrogens. The method demonstrated high accuracy and precision, and allowed simultaneous analysis of E1, E2, and E3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
You-Rim Lee, Seungyeon Lee, Sohyen Kwon, Jiyeong Lee, Hee-Gyoo Kang
Summary: Metabolomic research has been conducted in various fields, but the combination of forensic science and metabolomics is rare. This study identified and validated metabolite markers for determining the age of bloodstains within a week post-bleeding. These markers could be used as innovative tools for reconstructing crime scenes and suggesting initial investigative direction.
ANALYTICAL CHEMISTRY
(2022)
Article
Agronomy
Archis Pandya, Bjorn Thiele, Andres Zurita-Silva, Bjorn Usadel, Fabio Fiorani
Summary: The study evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions, showing considerable variability in saponin content in quinoa. Approximately 75% of the genotypes were classified as low-saponin content lines, which is promising for current and future quinoa breeding programs. Additionally, twelve saponins of the oleanane type were identified, including a previously unreported compound, contributing to the valorization of genetic resources for quinoa.
Article
Horticulture
Jung-Sung Chung, Hyeong-Cheol Kim, Su-Min Yun, Hyun-Jin Kim, Cheol-Soo Kim, Jeung-Joo Lee
Summary: Sulfur is an essential nutrient for plant growth and metabolism, and significantly affects the growth and metabolites of lettuce. Higher sulfur concentration treatment resulted in increased growth and metabolite content in lettuce compared to lower sulfur concentration treatment.
Article
Multidisciplinary Sciences
Hongjiang Xu, Yanan Li, Jing Zhang, Jinhua Zhang, Jianguang Lu, Xiquan Zhang, Ling Yang, Wenjie Zhao, Jun Feng
Summary: A rapid UHPLC-MS/MS assay method was developed for the determination of CMS E2 and colistin E2 in human plasma. The method demonstrated speed and high accuracy, and was successfully applied to the analysis of plasma samples obtained from Chinese healthy volunteers.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Applied
Ye Li, Jun Liang, Jia-Ning Gao, Yu Shen, Hai-Xue Kuang, Yong-Gang Xia
Summary: A new method combining liquid chromatography-tandem mass spectrometry (LC-MS/MS) and aldononitrile acetate derivatization (AND) has been developed for the comprehensive characterization of monosaccharide composition in plant derived polysaccharides. The optimized method can effectively separate 17 carbohydrates and is more powerful than traditional GLC-based methods for analyzing complex natural polysaccharides.
CARBOHYDRATE POLYMERS
(2021)
Review
Chemistry, Analytical
Lili Zhang, Na Li, Shengshuang Chen, Xiqing Bian, Mohamed A. Farag, Yahui Ge, Jianbo Xiao, Jian-Lin Wu
Summary: Carboxyl-containing compounds (CCCs) are crucial for the human body and their determination in food is challenging. Derivatization LC-MS technique plays a significant role in overcoming these challenges in food chemistry. This review comprehensively discusses CCCs in terms of structures, biological functions, and food sources, highlighting the importance of derivatization LC-MS as a powerful analytical tool.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Carl Jenkinson, Reena Desai, Malcolm D. McLeod, Jonathan Wolf Mueller, Martin Hewison, David J. Handelsman
Summary: This study analyzed the contribution of sulfate and glucuronide vitamin D metabolites relative to unconjugated levels in human serum. The results showed that sulfate conjugates formed a higher proportion of circulating vitamin D metabolites compared to glucuronide conjugates. The findings suggest that a combination of both conjugated and unconjugated measurements may provide a more accurate assessment of vitamin D status.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Review
Chemistry, Analytical
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: Glutathione, as the most abundant antioxidant in living organisms, plays a crucial role in cells due to its free thiol group. Its determination using liquid chromatography has been widely studied and proves significant in understanding the development of human diseases.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Ernesto de Jesus Zapata Flores, Nguyen Kim Ngan Bui, Sigrid Selberg, Koit Herodes, Ivo Leito
Summary: This study proposes derivatization reagents based on azobenzene, AzoB and AzoC, for LC-ESI-MS analysis of free amino acids in fermented beverages and juices. The results show that AzoB can be used for targeted and non-targeted analysis, while AzoC has advantages such as fast reaction and good reactivity with secondary amines but is affected by side products for quantitation.
Article
Microbiology
Lyubomir Dimitrov Stanchev, Iben Moller-Hansen, Pawel Lojko, Catarina Rocha, Irina Borodina
Summary: The transportome of Saccharomyces cerevisiae consists of membrane-bound proteins, but little is known about them. This study introduces a screening method using C-13-labeled stable isotopes and targeted LC-MS/MS to identify metabolite transporters in Saccharomyces cerevisiae. The results demonstrate the potential of this method in high-throughput screening for native metabolite transporters.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Maxime Meloche, Martin Jutras, Isabelle St-Jean, Simon de Denus, Gregoire Leclair
Summary: A sensitive LC-MS/MS assay was developed and validated for quantification of (S)-metoprolol and its metabolite in human plasma. The method showed stability and accuracy for clinical use, which could be further explored for investigating the clinical impact of (S)-MET concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Engineering, Environmental
Yu-Ning Hu, Jin-Tao Zhan, Pei-Rong Bai, Na An, Jun-Jie Tan, Yan-Zhen Wang, Quan- Fei Zhu, Yu-Qi Feng
Summary: In this study, a click chemistry-assisted mass spectrometry strategy was developed to profile the metabolites of DEHP in rats. By labeling and detecting the metabolites of DEHP, a total of 247 metabolites were successfully screened, including some previously unrevealed metabolites. The discovery of these new DEHP metabolites provides additional insights for understanding the metabolism of DEHP and exploring its toxicity mechanism in the future.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Agriculture, Multidisciplinary
Bo-Min Kim, Hyeon-Jeong Lee, Yeong H. Song, Hyun-Jin Kim
Summary: Increasing NaCl concentration led to decreased growth and mineral imbalance in spinach plants. NaCl irrigation resulted in decreased levels of most metabolites, affecting the nutritional quality of spinach.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemical Research Methods
I. G. Bressan, S. F. Llesuy, C. Rodriguez, A. Ferloni, A. R. Dawidowski, S. B. Figar, M. Gimenez
Summary: In 2015, glyphosate was classified as probably carcinogenic to humans by the International Agency for Research on Cancer, leading to increased public concerns about its environmental and health risks. A simple LC-MS/MS method was developed for quantification of glyphosate in human urine and successfully applied to 52 urine samples from exposed subjects in northern Argentina.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Qin C. Ji, Xiaohui Xu, Eric Ma, Jane Liu, Shenita Basdeo, Guowen Liu, William Mylott, David W. Boulton, Jim X. Shen, Bruce Stouffer, Anne-Francoise Aubry, Mark E. Arnold
ANALYTICAL CHEMISTRY
(2015)
Review
Biochemical Research Methods
Jim X. Shen, Guowen Liu, Yue Zhao
EXPERT REVIEW OF PROTEOMICS
(2015)
Article
Biochemical Research Methods
Long Yuan, Alan Schuster, Jim X. Shen, Pamela Garrison-Borowski, Anne-Francoise Aubry
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2015)
Article
Biochemical Research Methods
Yue Zhao, Guowen Liu, Aida Angeles, Lora L. Hamuro, Kevin J. Trouba, Bonnie Wang, Renuka C. Pillutla, Binodh S. DeSilva, Mark E. Arnold, Jim X. Shen
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2015)
Article
Biochemical Research Methods
Jane Liu, Long Yuan, Guowen Liu, Jim X. Shen, Anne-Francoise Aubry, Mark E. Arnold, Qin C. Ji
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2015)
Article
Biochemical Research Methods
Yue Zhao, Guowen Liu, Aida Angeles, Lisa J. Christopher, Zhaoqing Wang, Mark E. Arnold, Jim X. Shen
Article
Chemistry, Analytical
Guowen Liu, Yue Zhao, Aida Angeles, Lora L. Hamuro, Mark E. Arnold, Jim X. Shen
ANALYTICAL CHEMISTRY
(2014)
Article
Biochemical Research Methods
Yue Zhao, Guowen Liu, Hongwu Shen, Jim X. Shen, Anne-Francoise Aubry, Lakshmi Sivaraman, Mark E. Arnold
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2014)
Article
Biochemical Research Methods
Yue Zhao, Guowen Liu, Yifei Liu, Long Yuan, Dara Hawthorne, Jim X. Shen, Mausumee Guha, Anne Aubry
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2013)
Article
Biochemical Research Methods
Xiaohui (Sophia) Xu, Stephen R. Dueker, Lisa J. Christopher, Pete N. Lohstroh, Chi Fung (Anther) Keung, Kai (Kevin) Cao, Samuel J. Bonacorsi, Laura Cojocaru, Jim X. Shen, W. Griffith Humphreys, Bruce Stouffer, Mark E. Arnold
Article
Biochemical Research Methods
Xiaohui Xu, Qin C. Ji, Mohammed Jemal, Carol Gleason, Jim X. Shen, Bruce Stouffer, Mark E. Arnold
Article
Biochemical Research Methods
Janice Pursley, Jim X. Shen, Alan Schuster, Oanh T. Dang, Jim Lehman, Michael H. Buonarati, Yan Song, Anne-Francoise Aubry, Mark E. Arnold
Article
Biochemical Research Methods
Xiaohui (Sophia) Xu, Hao Jiang, Lisa J. Christopher, Jim X. Shen, Jianing Zeng, Mark E. Arnold
Editorial Material
Biochemical Research Methods
Yue Zhao, Guowen Liu, Jim X. Shen, Anne-Francoise Aubry
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)