Article
Biology
Marcelo de M. A. Coura, Eder A. Barbosa, Guilherme D. Brand, Carlos Bloch, Joao B. de Sousa
Summary: The incidence of colorectal cancer (CRC) is increasing in Brazil and there is a lack of reliable biomarkers for diagnosis and prognosis. Alterations in N-glycosylation profiles in serum and tissues of colon cancer patients were identified using mass spectrometry technologies. While serum N-glycans were mostly decreased, certain forms were overexpressed in tumor tissues. There was little correlation between tissue and serum N-glycosylation profiles in cancer patients. Total serum N-glycome analysis showed potential in discriminating patients from healthy controls, but compositions in serum did not parallel those found in tumor tissues.
Article
Chemistry, Analytical
Byeong Gwan Cho, Cristian D. Gutierrez Reyes, Mona Goli, Sakshi Gautam, Alireza Banazadeh, Yehia Mechref
Summary: Targeted mass spectrometric analysis, including techniques like selected reaction monitoring and multiple reaction monitoring, has been widely used in structural analysis of biomolecules. The recent innovation of parallel reaction monitoring (PRM) allows simultaneous detection of all product ions, simplifying the workflow and improving data quality. PRM offers advantages in reducing background interference and improving sensitivity, attributed to developments in sensitive mass analyzers like orbitrap and time of flight.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Yue Jia, Yu Lu, Xue Wang, Yuerong Yang, Meiyi Zou, Jie Liu, Wanjun Jin, Xiaoqin Wang, Guoliang Pang, Linjuan Huang, Zhongfu Wang
Summary: The study found that with prolonged lactation, the relative content of glycan derivatives of bovine lactoferrin changed, with the highest content observed in colostrum. Additionally, linkage-specific derivatization of sialic acid could distinguish between different sialylated N-glycan isomers.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Peilin Jiang, Wenjing Peng, Jingfu Zhao, Mona Goli, Yifan Huang, Yunxiang Li, Yehia Mechref
Summary: The complexity and heterogeneity of protein glycosylation present challenges in characterization and quantitation. This study introduces a metabolic isotope labeling strategy called GlyProSILC, which can label both the glycan motif and peptide backbone in the same batch of cells. It provides an accurate and effective quantitation approach for glycomics, proteomics, and glycoproteomics in a cell culture system.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Mengqi Luo, Tao Su, Qingyuan Cheng, Xue Zhang, Fei Cai, Zaiwen Yin, Fuping Li, Hao Yang, Fang Liu, Yong Zhang
Summary: In this study, the researchers used GlycoTCFM to profile the O-glycoproteome of human sperm and seminal plasma, revealing novel O-glycoproteins and their potential roles in sperm function and male infertility. The study also created a comprehensive O-glycoprotein database, providing valuable resources for further functional studies.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Plant Sciences
Hui Xu, Hang Yu, Jie Fu, Zheng-Wei Zhang, Jia-Chun Hu, Jin-Yue Lu, Xin-Yu Yang, Meng-Meng Bu, Jian-Dong Jiang, Yan Wang
Summary: This study revealed the interaction between plantamajoside and gut microbiota using high-resolution mass spectrometry and targeted metabolomics methods. The results showed that plantamajoside was rapidly metabolized into various active metabolites in the gut microbiota. Moreover, plantamajoside was found to affect short-chain fatty acid and tryptophan metabolism. These findings are significant for understanding the pharmacological effects of plantamajoside.
Article
Chemistry, Analytical
Byeong Gwan Cho, Peilin Jiang, Mona Goli, Sakshi Gautam, Yehia Mechref
Summary: This study investigated the viability of a newly commercialized micro array pillar nanoLC columns (mu PAC) for the analysis of permethylated glycans, demonstrating its efficient separation of different types of glycans and multiple advantages.
Article
Biochemical Research Methods
Rafael Maldonado-Hernandez, Orestes Quesada, Jose A. Gonzalez-Feliciano, Abel Baerga-Ortiz, Jose A. Lasalde-Dominicci
Summary: Cys-loop pentameric ligand-gated ion channels, including the nicotinic acetylcholine receptor (nAChR), are a dynamic group of proteins. Our study successfully produced a highly pure nAChR in detergent complex and conducted comprehensive research on its structure and function. Additionally, we analyzed the glycosylation in the nAChR subunits and identified high-mannose N-glycans.
Article
Agriculture, Multidisciplinary
Zhongyu Wang, Na Zhang, Wendan Wang, Yitong Li, Ignatius M. Szeto, Hongqiang Qin, Yan Jin, Mingliang Ye
Summary: The N-glycoproteomics analysis of human milk whey revealed changes in site-specific glycans without removing the attached glycans, providing insights into the role of protein N-glycosylation during infant development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Berfin Sucu, Ayse Bayraktar, Hatice Duman, Aysenur Arslan, Merve Kaplan, Melda Karyelioglu, Eda Ntelitze, Taner Tastekin, Seray Yetkin, Melih Erturk, Steven A. Frese, Bethany M. Henrick, Haci Mehmet Kayili, Bekir Salih, Sercan Karav
Summary: The study discusses the impact of protein glycosylation on gut microbiota and prebiotic production, and proposes a method to improve N-glycan isolation by recombinant production of novel enzymes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Construction & Building Technology
Stepanka Kuckova, Gabriela Rambouskova, Petra Junkova, Jiri Santrucek, Pavel Cejnar, Tatiana Anatolievna Smirnova, Ondrej Novotny, Radovan Hynek
Summary: The enrichment of mortar mixtures with natural organic materials has a long history, with protein additives proven to enhance mortar durability and strength. This study aimed to identify and quantify proteins in model mortars with various protein additives using mass spectrometry techniques. The results confirmed the possibility of identifying protein additives in century-old mortars, shedding light on traditional recipes.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Tyler J. Stewart, Kazuo Takahashi, Nuo Xu, Amol Prakash, Rhubell Brown, Milan Raska, Matthew B. Renfrow, Jan Novak
Summary: The nano-LC-MS analyses on clustered O-glycan biosynthesis on IgAl provide valuable insights into the enzymatic activities of glycosyltransferases and the effects of pre-existing glycans on subsequent activities. These findings have potential implications in understanding the molecular mechanisms involved in IgA nephropathy, and can be applied to the studies of other glycosyltransferases involved in mucin-type core 1 clustered O-glycosylation.
Article
Food Science & Technology
Nannan Li, Shuqin Li, Qirou Wang, Shuyu Yang, Yinuo Hou, Yan Gao, Xiangyang Zhang, Min Zhang, Haixia Chen
Summary: This study applied MALDI-TOF MSI and QTOF LC-MS/MS techniques to investigate the spatial distribution changes of low molecular weight compounds in garlic tissue during Laba garlic processing. It was found that some bioactive compounds were transformed or leached out during the processing, while new compounds related to pigments emerged. This study provides a basis for understanding the spatial distributions and changes of compounds in garlic tissue during Laba garlic processing, which may affect the bioactivities of garlic.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)