Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Susanne Huber, Klemens Losso, Gunther K. Bonn, Matthias Rainer
Summary: This study is the first to use DART-MS for the rapid quantification of cannabidiol (CBD) in CBD oils. The results show that the quantification of CBD by DART-MS is highly correlated with a validated LC-MS method. The relative standard deviation values of the DART-MS measurements are below the acceptable limit, demonstrating that DART-MS is a reliable and cost-effective alternative method for CBD oil quality control.
ANALYTICAL METHODS
(2022)
Article
Environmental Sciences
Alina T. Roman-Hubers, Thomas J. McDonald, Erin S. Baker, Weihsueh A. Chiu, Ivan Rusyn
Summary: This study examined the potential of IMS-MS as a high-throughput method for the chemical characterization of crude oils, finding it to be either equal or better than GC-MS in classifying the origins of crude oils and greatly increasing sample analysis throughput. The study demonstrated the utility of IMS-MS for rapid fingerprinting of complex samples and showed its advantages over traditional GC-MS-based analyses in emergency decision-making situations.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Review
Spectroscopy
Xiaoping Zhang, Vladimir Frankevich, Jianhua Ding, Yuanyuan Ma, Konstantin Chingin, Huanwen Chen
Summary: The direct mass spectrometry analysis of exhaled human breath has made significant progress in the past decade, including various methods such as selected ion flow tube mass spectrometry, proton transfer reaction mass spectrometry, and atmospheric pressure photoionization mass spectrometry. This article reviews recent developments in the analytical performance and applications of these methods in disease diagnosis, targeted molecular detection, and real-time metabolic monitoring.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Biochemical Research Methods
Robert B. Cody
Summary: Organic acid salts have low volatility and are not ideal for DART-MS analysis, but addition of a strong acid can convert them to the free acid form to facilitate qualitative analysis.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Markus Karjalainen, Anton Kontunen, Anna Anttalainen, Meri Makela, Soma Varga, Maiju Lepomaki, Osmo Anttalainen, Pekka Kumpulainen, Niku Oksala, Antti Roine, Antti Vehkaoja
Summary: Effective surgical margin assessment is crucial for good oncological outcomes, and new methods are being actively developed. One such method is the analysis of the chemical composition of surgical smoke to evaluate margins. In a simulated experiment using porcine tissues, we analyzed the delay in smoke transportation and evaluated its impact on tissue classification. The results showed that with a typical smoke evacuator setting, it takes about 1 second for the smoke to reach the sensor from the surgical plume.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Junhui Li, Rong Liu, Wenqing Gao, Jiancheng Yu, Keqi Tang
Summary: A detailed experimental characterization on ion storage biases in an ion funnel trap was conducted using cytochrome C and ubiquitin samples. It was observed that an ion overflow occurred when the capacity of the ion funnel trap was exceeded, resulting in a significant structural bias in the ion mobility spectrometry/time of flight mass spectrometry (IMS-TOF MS) spectrum. Increasing the RF voltage applied to the ion funnel trap amplified this effect. The specific charge state of the ions also played a role, with lower charge state ions showing a greater sensitivity to the ion structural bias.
Review
Chemistry, Analytical
Daniel G. Delafield, Gaoyuan Lu, Cameron J. Kaminsky, Lingjun Li
Summary: Mass spectrometry-based biomolecular analyses have become popular in research settings. Gas phase ion separation techniques provide a fourth dimension of analysis, enabling rapid characterization and high throughput profiling. This review highlights the latest developments and applications of high-end ion-mobility enabled instruments and their advantages for biomolecular research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Elena Canellas, Paula Vera, Cristina Nerin, Jeff Goshawk, Nicola Dreolin
Summary: Polyurethane adhesives used for bonding cork in champagne stoppers were found to contain non-intentionally added substances through ion mobility-time of flight-mass spectrometry analysis. Identified new compounds resulted from reactions of diisocyanates with acetic acid and ethanol, as well as malic acid or tartaric acid in champagne. Migration levels of these compounds exceeded the migration limit for Cramer class III compound.
Article
Chemistry, Applied
Xiaomin Li, Shuangqing Li, Hongmei Li, Jing Wang, Qin Luo, Xiong Yin
Summary: This study presents a robust method for simultaneous screening and quantitation of seven artificial sweeteners in alcoholic drinks using direct analysis in real time coupled with QTRAP mass spectrometry. The method is simple, linear, with low limit of quantification and accurate in monitoring the artificial sweeteners in various alcoholic beverages. The approach described could be suitable for large-scale application in routine quality control analysis of artificial sweeteners.
Article
Polymer Science
Torki A. Zughaibi, Robert R. Steiner
Summary: Polymeric fibers, particularly in carpet samples found at crime scenes, were analyzed using DART-AccuTOF(TM) method, successfully identifying and differentiating multiple polymer standards. The method was able to correctly identify known fiber compositions and confirm unknown compositions through FTIR, demonstrating its potential as a valuable addition to forensic laboratory analysis of carpet fibers.
Article
Chemistry, Analytical
Qingyu Hu, Yuting Sun, Xiyan Mu, Yulan Wang, Huiru Tang
Summary: Direct-infusion tandem mass spectrometry (DI-MS/MS) is valuable for quantitative metabolomics, MS imaging, and single cell studies, but cannot differentiate isomers/isobars with similar MS spectral features. In this study, the fragmentation pathways and characteristics of differential ion-mobility spectrometry (DMS) for four organic compounds were comprehensively investigated. A DI-DMS-MS/MS method was developed to simultaneously quantify these compounds. The method showed high accuracy and low limit-of-quantification, and clear differences between human and mouse urine samples were observed.
Article
Chemistry, Analytical
Aurelie Delvaux, Estelle Rathahao-Paris, Blanche Guillon, Sophie Cholet, Karine Adel-Patient, Francois Fenaille, Christophe Junot, Sandra Alves
Summary: The study introduces a high throughput and high resolution method for characterizing main oligosaccharides in breastmilk using ion mobility spectrometry, offering high identification level. Standard HMO isomers were investigated and could be distinguished effectively. Multiple mobility peaks were observed for some HMO species, but reduction at the reducing-end did not significantly improve isomer distinction.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Tyler S. Larson, Thomas J. Diprospero, Gary L. Glish, Matthew R. Lockett
Summary: In this study, a novel method was used to investigate the lipid profile changes in oxaliplatin-sensitive and resistant cells, revealing significant increases in triglycerides and cholesterol esters caused by oxaliplatin, which were correlated with lipid droplet formation.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Min Liu, Simin Wang, Wuxia Ge, Wentao Bi, David Da Yong Chen
Summary: This study systematically investigated the influence of host-guest interactions on the analytical performance of direct analysis in real time mass spectrometry (DART-MS) by studying the interactions between cyclodextrins (CDs) and Sudan dyes. The results showed that the host-guest interaction between CDs and Sudan dyes affected the quantitative analysis of the target compounds by lowering the signal intensity. Furthermore, the addition of organic solvents weakened the host-guest interaction and improved the signal intensity in DART-MS, particularly in solid-phase microextraction (SPME).
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)