Article
Engineering, Environmental
Shunxiang Gao, Yajun Cheng, Shenghai Zhang, Xin Zheng, Jihong Wu
Summary: This study presents a biosensor-based detection method for sensitive, specific, and rapid monitoring of TNF-alpha. The biosensor exhibited a wide detection range and good reproducibility, making it a promising approach for clinical applications.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Huimin Li, Jiaxing Di, Baowei Peng, Yuhong Xu, Ning Zhang
Summary: The study found that immunoliposomes may bind either monovalently or multivalently, mainly determined by the surface ligand density rather than the antibody valency. The valency of immunoliposomes correlated with the dissociation rate constant (K-off), but not with the association rate constant (K-on), and their binding capabilities did not necessarily increase with the surface anti-CD3 valency.
PHARMACEUTICAL RESEARCH
(2021)
Article
Fisheries
Chloe J. English, Angus Li, Andrew C. Barnes
Summary: This study attempted to optimize a single-step enzyme-linked immunosorbent assay (ELISA) using an Octet HTX instrument to quantify IgM specific to the hapten 2,4-dinitrophenol (DNP) in serum from Atlantic salmon. The assay proved to be a robust, repeatable, and high-throughput alternative to ELISA.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biophysics
Xiao Liu, Chunzheng Huang, Cao Qiu, Zijing Wang, Mengjun Cheng, Yan Zhang, Yinghan Qiao, Yuan Guan, Xin Feng, Changjiang Sun, Na Li, Fengyang Li, Liancheng Lei, Wenyu Han, Xiaoguang Zhang, Jingmin Gu
Summary: In this study, a novel method for the detection of S. aureus was established using the C54A mutant LysGH15 as the receptor in combination with BLI. This method can directly assay S. aureus whole cells with high specificity and distinguish between viable and dead S. aureus.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Multidisciplinary
Yi Tao, Lin Chen, Meiling Pan, Fei Zhu, Dong Zhu
Summary: Biosensors have shown significant developments in drug discovery, aiding in the screening and discovery of innovative drugs. This overview discusses recent progress in tailored biosensors, highlighting their mechanisms and applications in drug screening, efficacy assessment, and toxicity evaluation, as well as the incorporation of functional nanomaterials. Representative examples of different biosensor types are compared in terms of design strategy, response mechanism, and potential applications, demonstrating the challenges and prospects of biosensors-assisted drug discovery.
Article
Fisheries
Angus Li, Richard J. Harris, Bryan G. Fry, Andrew C. Barnes
Summary: Quantification of specific IgM for an antigen and determination of its avidity can be robustly and reproducibly achieved through BLI, offering a faster, more precise, and more repeatable alternative to ELISA. The BLI data showed consistent results, indicating that technical triplicates were unnecessary.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Allergy
Kai M. T. Sauerwein, Christoph B. Geier, Roman F. Stemberger, Raphael Rossmanith, Huseyin Akyaman, Peter Illes, Michael B. Fischer, Martha M. Eibl, Jolan E. Walter, Hermann M. Wolf
Summary: This study analyzed the production of IgG antibodies in a subgroup of CVID patients following mRNA vaccination with BNT162b2 SARS-CoV-2 vaccine. The results showed that CVID responders had lower levels of antibodies and impaired affinity maturation during booster response. Additionally, the follicular T-helper cell response was significantly reduced in CVID responders compared to healthy individuals.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Sumin Bian, Ying Tao, Zhoule Zhu, Peixi Zhu, Qiqin Wang, Hemmings Wu, Mohamad Sawan
Summary: Developed two types of fiber-optic biolayer interferometry (FO-BLI) biosensors for on-site carbamazepine (CBZ) monitoring, indirect and direct types. The direct FO-BLI biosensor achieved accurate and efficient detection of CBZ concentration in a short period of time, with good validation results.
Article
Engineering, Environmental
Shunxiang Gao, Qian Li, Shenghai Zhang, Xinghuai Sun, Hong Zhou, Yun Zhang, Jihong Wu
Summary: Precisely monitoring biomarkers is crucial for early disease warning, diagnosis, and prognosis. However, detecting targets with high sensitivity and specificity using interface-based biosensing methods remains challenging due to limited surface area and single kinetic responsiveness. In this study, a biolayer interferometry-based peptide-nucleic acid aptamer pair (BLI-PNAP) biosensor is introduced for specific monitoring of disease biomarkers at picomolar concentrations. The biosensor achieves good responsiveness by increasing molecular assembly density, ultra-high specificity through molecular dynamics selectivity, and enhanced sensitivity by introducing gold nanoparticles for signal amplification. The BLI-PNAP biosensor demonstrates a wide linear range, high sensitivity, and excellent performance in clinical serum samples, offering potential for point-of-care diagnosis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Microbiology
Da-Wei Zhang, Xiao-Shuang Xu, Rui Zhou, Zhiguo Fu
Summary: The natural compounds SA and SB, derived from medicinal plants, were found to bind to the CA CTD pocket of HIV-1 and exhibit antiviral effects. They were also shown to enhance CA assembly, which suggests their potential as chemical tools for modulating HIV-1 CA assembly.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Da-Wei Zhang, Liangxu Xie, Xiao-Shuang Xu, Yimin Li, Xiaojun Xu
Summary: The study identified protoporphyrin IX (PPIX) as a potential modulator of HIV-1 capsid protein (CA), promoting CA assembly and preferentially binding the CA hexamer. PPIX acts as a glue at the interfaces between CA subunits to accelerate multimerization. This discovery suggests that PPIX could be used as a new lead for CA modulation and has potential research applications.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Biomedical
Shunxiang Gao, Qian Li, Shenghai Zhang, Xinghuai Sun, Hong Zhou, Zhongfeng Wang, Jihong Wu
Summary: Glaucoma is a major cause of blindness worldwide and early detection and intervention are crucial for its prognosis. Monitoring the biomarker GDF15 allows for precise diagnosis and assessment of glaucoma, and this study demonstrates that it can also aid in screening for glaucoma risk and early diagnosis.
Article
Food Science & Technology
Xiaojuan Zhang, Yun Gao, Bowen Deng, Bo Hu, Luming Zhao, Han Guo, Chengfang Yang, Zhenxia Ma, Mingjuan Sun, Binghua Jiao, Lianghua Wang
Summary: This study successfully obtained an aptamer with high affinity and specificity for the challenging marine biotoxin GYM-A through Capture-SELEX, and established a novel aptasensor based on BLI technology for the detection of GYM-A, showing excellent performance.
Article
Chemistry, Analytical
Li Luo, Hua Xu, Chunyan Wang, Zhi Li, Lei Guo, Xinying Li, Longlong Luo, Jing Wang, Weijun Kang, Jianwei Xie
Summary: The increasing global threat of biotoxins has emphasized the challenges in the initial response, making the implementation of sensitive, specific, and rapid sensing methods highly valuable. This article explores the application of a double amplification strategy using immuno-gold nanoparticles in BLI and demonstrates its potential in measuring trace ricin.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Medicine, Research & Experimental
Maria U. Johansson, Christopher Weinert, Dietrich Alexander Reichardt, Dana Mahler, Dania Diem, Christian Hess, Diana Feusi, Simon Carnal, Julia Tietz, Noreen Giezendanner, Fabio Mario Spiga, David Urech, Stefan Warmuth
Summary: Upon reformatting antibody to single-chain variable fragment format, a previously hidden hydrophobic patch in the variable/constant domain interface becomes accessible for preexisting anti-drug antibody binding. To reduce the reactivity of preexisting antibodies and the exposed hydrophobic patch, mutations were introduced in this region. Through computational methods and experimental characterization, mutation of two threonine residues, Thr101 and Thr146, was found to be critical in eliminating preexisting antibody reactivity, which could have important implications in optimizing early drug development for antibody fragment-based therapeutics.
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)