Article
Biochemical Research Methods
Katerina Cokrtova, Vit Mares, Tomas Krizek
Summary: The feasibility of on-capillary derivatization of saccharides by aromatic amine-based fluorescent labeling agents was tested. Glucose was used as a model analyte and the direct reaction of the saccharide with the labeling agent in acetic acid yielded a stable labeled product that could be separated and detected effectively. The method showed excellent linearity and repeatability for a mixture of different types of saccharides.
Article
Biochemical Research Methods
Jana Krenkova, Filip Dusa, Richard Cmelik
Summary: In this study, a multi-cationic aminopyrene-based labeling tag was characterized for oligosaccharide analysis by capillary electrophoresis with laser-induced fluorescence detection. The APTMP labeling showed high reproducibility but lower reactivity compared to the commonly used APTS labeling. APTMP labeling remains promising for oligosaccharide labeling followed by CE-MS in a positive ion mode.
Article
Biochemical Research Methods
Lisa A. Holland, Yan He, Jessica R. Guerrette, Cassandra L. Crihfield, Lloyd Bwanali
Summary: An automated and cost-effective method based on capillary gel electrophoresis laser-induced fluorescence detection was developed for quantification of trace levels of plasmid topoisomers. The method has broad applicability for laboratories requiring routine analyses of plasmid topology for the development of new plasmid-based therapies and quality control of gene therapies utilizing supercoiled DNA. The detection of topoisomers was achieved by incorporating ethidium bromide in the separation medium, with improved detector response using a specific optical filter.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zaifang Zhu, Mark Lies, John Silzel
Summary: Using a laser driven light source for excitation, a native fluorescence detection (NFD) scheme for protein analysis was reported in this study. The results suggest that CE-NFD is a practical and powerful tool for protein characterization in the biopharmaceutical industry.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Denisa Smolkova, Richard Cmelik, Jana Lavicka
Summary: Capillary electrophoresis is widely used for oligosaccharide and glycan analysis, but the low electrophoretic mobility and lack of chromophore or fluorophore in saccharide molecules require modification before separation and detection. This review summarizes the labeling strategies used for oligosaccharide/glycan analysis, categorized based on the labeling mechanism and saccharide molecule. In addition to the commonly used 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, less common and recently developed tags applicable in glycomics are also summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
ShengHao Chen, YanLan Liang, YuanYuan Li, Yue Sun, Yang He
Summary: A microchip capillary electrophoresis method combined with laser-induced fluorescence detection was developed for separation and detection of physcion and rhein in rhubarb. The optimized conditions included using ammonium acetate-dimethyl sulfoxide as the buffer system, with a ratio of 60/20/20 (v/v/v) of hydro-organic solvent, and a separation voltage of 1.9 kV. The method showed good linearity, low limits of detection, satisfactory precision and accuracy. It is a simple, efficient, fast, sensitive, and low-cost method.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Ziyan Yu, Yaxiao Liao, Jie Liu, Qin Wu, Yu Cheng, Ke Huang
Summary: A novel colorimetric approach for detecting kanamycin based on the aggregation of gold nanoparticles (AuNPs) induced by kanamycin is presented in this study. The sensor showed a linear range of 0.5-3 nM and a limit of detection of 0.11 nM under optimal conditions. It was tested by monitoring kanamycin in six food samples with satisfactory spiked recoveries, making it a desirable choice for fast and highly sensitive detection of kanamycin.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yuanhang Wang, Jing Wu, Ping Sun, Jie Yu, Qiaosheng Pu
Summary: This study successfully developed a fully functional palmtop MCE analyzer by integrating multiple units and using 3D printing for fabrication. The device is characterized by its small size, lightweight, and low cost, and can be powered by a laptop or mobile power bank.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Mingxia Liu, Lixia Chen, Xiangjun Li, Jinghua Meng, Yu Bai, Huwei Liu
Summary: A chiral analytical method based on capillary electrophoresis and laser-induced fluorescence detection was developed for the simultaneous separation and detection of four stereoisomers of 3-hydroxyaspartate. By utilizing microwave-assisted derivatization and online sample concentration methods, the sensitivity was improved significantly, resulting in excellent analytical performance.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Yuling Li, Bowen Zhou, Keke Wang, Jing Zhang, Wenjian Sun, Li Zhang, Yinlong Guo
Summary: The mass-tagged chiral selector [d(0)]/[d(5)]-17 beta-EBC was successfully developed for the separation and identification of amino acid enantiomers, achieving good enantiomeric resolution for 19 chiral proteinogenic AAs in a quick analytical run. The linear calibration curve of enantiomeric excess using [d(0)]/[d(5)]-17 beta-EBC showed promising results, demonstrating its potential in peptide drug quality control and discovery of chiral disease biomarkers.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Yejing Huang, Xiufen Han, Xiao Yu, Shumei Wang, Haiyun Zhai
Summary: A capillary electrophoresis-indirect laser-induced fluorescence detection method was established for neomycin detection in fish. With optimal conditions, the limit of detection of neomycin was 5.0 ng/g, the recovery of fish samples is between 95.2 and 99.7%, and the relative standard deviation is between 2.7 and 3.6%. The study also investigated the effects of background buffer, pH, and separation voltage on the separation and analysis process.
Article
Chemistry, Analytical
Takashi Anazawa, Motohiro Yamazaki, Shuhei Yamamoto, Ryoji Inaba
Summary: An ultra-small, highly efficient spectral-detection system was developed for detecting fluorescence from four emission points, allowing for highly sensitive and low-crosstalk seven-color detection. The system enables simultaneous quantification of up to seven fluorophores in each capillary, demonstrated through Sanger DNA sequencing and STR genotyping experiments.
Article
Biochemical Research Methods
Jianing Liu, Minmin Li, Xiaojuan Liu, Jing Huang, Li Yang
Summary: In this study, a robust and sensitive method for OGT assay based on CE-LIF method was developed. The method was successfully applied to detect OGT activity in clinical blood samples with a low limit of detection and satisfactory accuracy.
Article
Chemistry, Analytical
Jingjing Xue, Yiren Cao, Genhan Zha, Zixian Yu, Yuxin Wang, Weiwen Liu, Jicun Ren, Hua Xiao, Qiang Zhang, Chengxi Cao, Li Wei
Summary: In this study, a quadruple UV LED array was designed to directly irradiate gel electrophoresis chips at 275 nm. Simple detection setup using a 365 nm filter, UV camera lens, and CCD was integrated with the small gel electrophoresis chip to construct the IFI-GEC device. The LED array exhibited good evenness and enabled online imaging of the entire gel electrophoresis chip. The reproducible IFI-GEC detection was completed within 10 minutes with a low LOD of 40 ng for lysozyme detection, demonstrating the potential of the IFI-GEC device for stain-free online and portable protein detection.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Meagan R. Moser, Claire M. Smith, Genoveve G. Gutierrez, Christopher A. Baker
Summary: This article describes the design and development of a 3D printed instrument for Taylor dispersion analysis (TDA), which utilizes a fully 3D printed eductor as a vacuum source for pressure-driven flow. The instrument also features a dual-point laser-induced fluorescence (LIF) detection system with high sensitivity. The utility of the instrument is demonstrated through the sizing of R-Phycoerythrin at pM concentrations.
ANALYTICAL CHEMISTRY
(2022)
Article
Biology
Gergely Zachar, Tamas Jako, Istvan Vincze, Zsolt Wagner, Tamas Tabi, Eszter Balint, Szilvia Mezey, Eva Szoko, Andras Csillag
ACTA BIOLOGICA HUNGARICA
(2018)
Article
Biochemistry & Molecular Biology
Bence Szilagyi, Peter Kovacs, Gyorgy G. Ferenczy, Anita Racz, Krisztina Nemeth, Julia Visy, Pal Szabo, Janez Ilas, Gyorgy T. Balogh, Katalin Monostory, Istvan Vincze, Tamas Tabi, Eva Szoko, Gyorgy M. Keseru
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Article
Pharmacology & Pharmacy
Zsofia Ulakcsai, Fruzsina Bagamery, Eva Szoko, Tamas Tabi
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2018)
Article
Medicine, General & Internal
Laszlo-Istvan Baba, Melinda Kolcsar, Imre Zoltan Kun, ZsOfia Ulakcsai, Fruzsina Bagamery, Eva Szoko, Tamas Tabi, Zsolt Gall
MEDICINA-LITHUANIA
(2019)
Review
Clinical Neurology
Tamas Tabi, Laszlo Vecsei, Moussa B. Youdim, Peter Riederer, Eva Szoko
JOURNAL OF NEURAL TRANSMISSION
(2020)
Article
Clinical Neurology
Fruzsina Bagamery, Kamilla Varga, Kitti Kecsmar, Istvan Vincze, Eva Szoko, Tamas Tabi
JOURNAL OF NEURAL TRANSMISSION
(2020)
Article
Biochemistry & Molecular Biology
Fruzsina Bagamery, Kamilla Varga, Kitti Kecsmar, Istvan Vincze, Eva Szoko, Tamas Tabi
Summary: The study revealed that the impact of STZ on insulin signaling in differentiated SH-SY5Y cells differed from undifferentiated cells. Differentiation reduced sensitivity to the non-specific cytotoxicity of STZ and emphasized the development of insulin resistance.
NEUROCHEMICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Kornel Kiraly, David A. Karadi, Ferenc Zador, Amir Mohammadzadeh, Anna Rita Galambos, Mihaly Balogh, Pal Riba, Tamas Tabi, Zoltan S. Zadori, Eva Szoko, Susanna Furst, Mahmoud Al-Khrasani
Summary: There is ongoing debate on the effectiveness of opioids in the management of neuropathic pain, with dose escalation required to maintain analgesia leading to increased side effects. Literature suggests that angiotensin and its receptors may have an impact on pain transmission. The interaction between MOR and angiotensin receptors remains understudied in chronic pain, particularly neuropathy.
Article
Biology
Robert Gaspar, Judit Hajagos-Toth, Annamaria Schaffer, Anna Kothencz, Lilla Siska-Szabo, Eszter Ducza, Adrienn Csanyi, Tamas Tabi, Fruzsina Bagamery, Eva Szoko, Orsolya Kovacs, Tamara Barna, Reza Samavati, Mohsen Mirdamadi, Anita Sztojkov-Ivanov, Kalman Ferenc Szucs, Sandor G. Vari
Summary: This study investigates the effects of obesity on pregnant uterine contraction in a rat model and finds that obesity reduces uterine contractility and cytokine-induced inflammatory processes, demonstrating partial applicability of obese pregnant rats as a model for human processes.
Article
Biochemistry & Molecular Biology
Orsolya Geda, Tamas Tabi, Peter P. Lakatos, Eva Szoko
Summary: This study investigated the effects of DIMEB, HPBCD, RAMEA, and HPACD on ganglioside and cholesterol levels in rat brain synaptosomes. HPBCD showed selective cholesterol depletion capability, while RAMEA and HPACD selectively depleted gangliosides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Peter P. Lakatos, David Arpad Karadi, Anna Rita Galambos, Nariman Essmat, Kornel Kiraly, Rudolf Laufer, Orsolya Geda, Zoltan S. Zadori, Tamas Tabi, Mahmoud Al-Khrasani, Eva Szoko
Summary: Tolperisone, a centrally acting muscle relaxant, shows promising effects in alleviating neuropathic pain, restoring paw pressure threshold and glutamate levels in cerebrospinal fluid. Its mechanism of action primarily involves the blockade of ion channels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Nariman Essmat, Anna Rita Galambos, Peter P. Lakatos, David Arpad Karadi, Amir Mohammadzadeh, Sarah Kadhim Abbood, Orsolya Geda, Rudolf Laufer, Kornel Kiraly, Pal Riba, Zoltan S. Zadori, Eva Szoko, Tamas Tabi, Mahmoud Al-Khrasani
Summary: The current treatment of neuropathic pain is unsatisfactory, and new effective drugs or combination therapies are needed. This study tested the antinociceptive effect of oral tolperisone, pregabalin, and duloxetine against rat partial sciatic nerve ligation-induced tactile allodynia. Tolperisone and pregabalin showed a chronic antiallodynic effect, and the combination of tolperisone and pregabalin acutely alleviated allodynia and restored elevated CSF glutamate content.
Article
Respiratory System
Balazs Csoma, Andras Bikov, Lajos Nagy, Bence Toth, Tamas Tabi, Gergo Szucs, Zsolt Istvan Komlosi, Veronika Mueller, Gyoergy Losonczy, Zsofia Lazar
RESPIRATORY RESEARCH
(2019)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
