Article
Biochemistry & Molecular Biology
Heli Pan, Shiheng Lyu, Yanqiong Chen, Shirong Xu, Jianwen Ye, Guixin Chen, Shaohua Wu, Xiaoting Li, Jianjun Chen, Dongming Pan
Summary: The early-ripening cultivar 'Liuyuezaoyou' of Citrus grandis exhibits significant differences in phytohormones, sugar compounds, and organic acids compared to the wild type. Through high-throughput sequencing and RT-PCR, it was discovered that miRNAs play an important role in regulating the ripening process of Citrus grandis fruit, forming a complex network with transcriptomes. Further analysis revealed intricate interactions of miRNAs with mRNA targets, providing insights into the mechanisms underlying fruit ripening in this species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Mei Deng, Yaosheng Lin, Lihong Dong, Xuchao Jia, Yaolan Shen, Lei Liu, Jianwei Chi, Fei Huang, Mingwei Zhang, Ruifen Zhang
Summary: This study measured the properties of insoluble and soluble dietary fiber from citrus fruits, finding that Shatianyu had the highest fiber content and moderate to high levels of properties, potentially providing stronger health benefits.
Article
Chemistry, Applied
Mei Deng, Xuchao Jia, Lihong Dong, Lei Liu, Fei Huang, Jianwei Chi, Qin Ma, Dong Zhao, Mingwei Zhang, Ruifen Zhang
Summary: The Citrus genus is a good source of dietary flavonoids, with Shatianyu pulp containing various flavonoid compounds, among which naringin and rhoifolin exhibit high antioxidant activity. The structure of flavonoids also affects their antioxidant properties.
Article
Food Science & Technology
Mei Deng, Ruifen Zhang, Shuai Zhang, Qi Lu, Lihong Dong, Fei Huang, Xuchao Jia, Qin Ma, Jianwei Chi, Dong Zhao, Shijuan Yan, Mingwei Zhang, Citrus Grandis L. Osbeck
Summary: This study investigated the profiles and bioactivity of flavonoids in different tissue parts of Shatianyu (Citrus grandis L. Osbeck), including flavedo, albedo, septum, pulp, and seed. Fourteen flavonoids were identified using UHPLC-ESI-MS/MS in a multiple reaction monitoring (MRM) mode. The main flavonoids in flavedo, albedo, septum, and pulp were melitidin, naringin, and bergamjuicin, while naringin, melitidin, and neoeriocitrin were the major flavonoids in seed. Additionally, new structure flavonoids isolated from Shatianyu pulp, such as cigranoside C, D, E, F, bergamjuicin, and neoeriocitrin, were detected in all tissue parts. The flavedo and seed of Shatianyu showed potent pancreatic lipase inhibitory activity, indicating their potential for application in anti-obesity nutraceutical food.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Applied
Mei Deng, Lihong Dong, Xuchao Jia, Fei Huang, Jianwei Chi, Zafarullah Muhammad, Qin Ma, Dong Zhao, Mingwei Zhang, Ruifen Zhang
Summary: This study investigated the flavonoid profiles and bioactivity of commonly consumed pomelo and grapefruit cultivars in China. The results showed that both pomelo and grapefruit contained a variety of flavonoids, and exhibited strong antioxidant activity and pancreatic lipase inhibitory activity. These fruits are natural antioxidants and have anti-obesity potential.
Article
Plant Sciences
Arun Kumar Gupta, Subhamoy Dhua, Partha Pratim Sahu, Giulia Abate, Poonam Mishra, Andrea Mastinu
Summary: This study investigated the changes in phytochemical compounds in different parts of pomelo fruit during two consecutive growing seasons, revealing a systematic metabolism of these compounds during fruit development. The results have implications for the food processing industry in producing high-quality foods and introducing health-beneficial products into daily diets.
Article
Agronomy
Xiaodong Liu, Chengxiao Hu, Muhammad Riaz, Xiaoman Liu, Xuecheng Sun, Mulai Zhuang, Qiling Tan
Summary: Due to excessive fertilization, the fruit quality of the Guanxi pomelo has declined. The study found that high-quality fruit had higher levels of magnesium, iron, zinc, and boron, while lower levels of manganese and copper compared to sub-quality, common, and inferior fruit. Acid soils and low organic matter content led to imbalanced nutrient uptake, affecting fruit quality.
Article
Agronomy
Tengfei Pan, Muhammad Moaaz Ali, Jiangmei Gong, Wenqin She, Dongming Pan, Zhixiong Guo, Yuan Yu, Faxing Chen
Summary: The present study measured the fruit physiology and sugar-acid ratio of 24 pomelo cultivars grown in ten different locations of the subtropical region of China. The results showed the range of basic quality attributes and sugar-acid contents of these pomelo cultivars, as well as the characteristics of some cultivars with higher citric acid and pyruvate contents.
Article
Biochemistry & Molecular Biology
Li-Yun Lin, Chiung Chi Peng, Yi-Ping Huang, Kuan-Chou Chen, Robert Y. Peng
Summary: This study found that the waste of Citrus grandis Osbeck cv. Mato Peiyu fruits contains rich functional chemicals, including essential oil, p-synephrine, polyphenolics, and dietary fiber. The content of p-synephrine can serve as a marker of the internal maturation of these fruits.
Article
Biochemistry & Molecular Biology
Yu-Cheng Yeh, Lih-Shiuh Lai
Summary: The study investigated the structural and functional properties of Citrus grandis Osbeck seed mucilage obtained through different extraction methods. The findings revealed that CGO seed could serve as an excellent source of high methoxyl pectin with high intrinsic viscosity and molecular weight. The UAEE method with Celluclast(R) 1.5 L showed the highest extraction yield. EAE with cellulase had a negative impact on the purity of pectin. Overall, EAE with Celluclast(R) 1.5 L, followed by UAE and UAEE with Celluclast(R) 1.5 L, were the least affected extraction methods.
Article
Chemistry, Applied
Chuying Chen, Zhengpeng Nie, Chunpeng Wan, Zengyu Gan, Jinyin Chen
Summary: Chitosan coating has been proven to effectively suppress juice sac granulation and fruit senescence in pummelo fruits, by inhibiting lignin synthesis-related enzymes and enhancing the activities of cell wall degrading enzymes, ultimately maintaining cell wall modification and fruit quality.
Article
Food Science & Technology
Vernabelle Balmori, Marisa Marnpae, Charoonsri Chusak, Kritmongkhon Kamonsuwan, Kasinee Katelakha, Suvimol Charoensiddhi, Sirichai Adisakwattana
Summary: This study found that fermenting pomelo with Lacticaseibacillus paracasei can increase the count of viable lactic acid bacteria, organic acids, total polyphenols, and flavonoids, thereby enhancing its antioxidant activity and various functional attributes.
Article
Food Science & Technology
Sophia Chanu Warepam, Sujata Jena
Summary: This study investigated the effect of pomelo juice foam composition on foam properties and optimized the formulation for the production of high-quality stable foam-dried pomelo juice powder. The findings provide potential applications for the powder in bakery products, ice cream, and yogurt.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Food Science & Technology
Tuo Leng, Xiaoyi Hu, Yi Chen, Bei Gan, Jianhua Xie, Qiang Yu
Summary: Rapid identification and quantification of pork and duck meat adulterated in ground beef were successfully achieved using 1H NMR spectra combined with chemometrics. Principal component analysis (PCA) revealed that beef had higher levels of methionine and glutamine, which could serve as key markers for differentiating beef from pork and duck. Orthogonal partial least squares discriminant analysis (OPLS-DA) effectively discriminated between binary and ternary adulterated beef with 100% accuracy. Leucine, isoleucine, methionine, and glutamine were identified as potential markers for beef adulteration. Validation with external samples demonstrated the accuracy of the partial least squares (PLS) model in predicting levels of binary and ternary adulteration in beef, with determination coefficients R2p of 0.9153 and 0.9348, respectively, and root mean square error (RMSEP) of 0.1112 and 0.0821, respectively. These findings provide valuable insights into beef adulteration and enhance our understanding of the relationship between metabolites and meat.
Article
Food Science & Technology
Thuong Nhan Phu Nguyen, Chi Khang Van, Thu Trang Thi Nguyen, Thuan Van Tran, Quang Binh Hoang, Long Giang Bach
Summary: This study evaluated the encapsulation of pomelo essential oils using the spray drying technique. The optimal parameters were found to be a maltodextrin concentration of 30% (wt%/wt%), essential oil concentration of 1.5% (wt%/wt%), inlet temperature of 140 degrees C, and feed flow rate of 120 mL/h. These parameters resulted in the highest drying yields, microencapsulation yield, and microencapsulation efficiency.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)