Article
Veterinary Sciences
Qiangde Duan, Shengmei Pang, Lili Feng, Jiaqi Liu, Linfen Lv, Baoliang Li, Yuxuan Liang, Guoqiang Zhu
Summary: Heat-labile enterotoxin enhances bacterial adherence by upregulating adhesin and enterotoxin expression in enterotoxigenic Escherichia coli. These findings provide insights into the pathogenic mechanism of heat-labile enterotoxin and enterotoxigenic Escherichia coli.
VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Alberto Prieto, Cynthia Lopez-Novo, Pablo Diaz, Jose Manuel Diaz-Cao, Gonzalo Lopez-Lorenzo, Claudia Anton, Susana Remesar, David Garcia-Dios, Ceferino Lopez, Rosario Panadero, Pablo Diez-Banos, Patrocinio Morrondo, Gonzalo Fernandez
Summary: Neonatal calf diarrhoea is a significant issue in cattle production worldwide, with enterotoxigenic Escherichia coli (ETEC) being one of the major pathogens. Veterinary surgeons commonly use antimicrobials to treat the disease, but this study found that all ETEC strains were resistant to three or more families of antimicrobials. Only a few antibiotics showed efficacy against most ETEC strains, while many strains were resistant to first-line antimicrobials used in veterinary practice. The study emphasizes the importance of performing antimicrobial susceptibility tests in each outbreak to ensure effective treatment and prevent the emergence of new resistance mechanisms.
Review
Immunology
Alaullah Sheikh, James M. Fleckenstein
Summary: Pathogenic Escherichia coli can be classified into specific variants (pathovars) based on their behavior and the expression of specific virulence factors. These pathogens interact with CEACAMs through both core elements common to all E. coli and pathovar-specific virulence traits that target the IgV regions of CEACAMs. Recent data suggests that these interactions may not only benefit the pathogen but also provide a mechanism for pathogen elimination.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Matej Hrala, Juraj Bosak, Lenka Micenkova, Jitka Krenova, Matej Lexa, Viktoria Pirkova, Zuzana Tomastikova, Ivana Kolackova, David Smajs
Summary: The study identified three potentially probiotic E. coli strains producing antimicrobial compounds that effectively inhibit pathogenic E. coli strains, showing promising results for preventing infections during the postweaning period of piglets.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Melibea Berzosa, Alzbeta Nemeskalova, Amaia Zuniga-Ripa, Miriam Salvador-Bescos, Eneko Larraneta, Ryan F. Donnelly, Carlos Gamazo, Juan M. Irache
Summary: Enterotoxigenic Escherichia coli (ETEC) infections are a major cause of acute diarrhea in children in developing countries and a common cause of diarrhea in international travelers. This study investigates the immune response induced by a recombinant LTB unit incorporated in microneedles (rLTB-MN) in mice.
Article
Biotechnology & Applied Microbiology
Pengpeng Xia, Yunping Wu, Siqi Lian, Guomei Quan, Yiting Wang, Guoqiang Zhu
Summary: The deletion of the FaeG subunit attenuates clinical symptoms and intestinal mucosal damage caused by F4ac infection, as well as reduces apoptosis in intestinal epithelial cells induced by F4ac. Targeted depletion of FaeG remains beneficial for the prevention or treatment of F4ac infection.
Article
Microbiology
Ian E. Hollifield, Natalya I. Motyka, Kaylynn A. Fernando, Jacob P. Bitoun
Summary: Enterotoxigenic E. coli (ETEC) is a common cause of secretory diarrhea in children and adults. The ability of ETEC to survive within macrophages and their interaction with the host immune system is not well understood.
Article
Microbiology
Ipshita Upadhyay, Kathryn L. Lauder, Siqi Li, Galen Ptacek, Weiping Zhang
Summary: Currently, there is no licensed vaccine for enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in developing countries and among travelers. A protein-based multivalent vaccine candidate called MecVax has been shown to induce antibodies that neutralize ETEC toxins and inhibit bacterial adherence. In this study, adult rabbits immunized with MecVax showed a significant reduction in intestinal colonization by ETEC, suggesting that MecVax may be an effective vaccine candidate against ETEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Melibea Berzosa, Alberto Delgado-Lopez, Juan Manuel Irache, Carlos Gamazo
Summary: This study focuses on Enterotoxigenic Escherichia coli (ETEC), a major concern in low-income countries with no licensed vaccine available. Bacterial vesicle-based vaccines show promise due to their safety and diverse virulence factors. The proposed method involves heat treatment and ultrafiltration to recover vesicles from bacterial cultures, and preliminary findings suggest their suitability for an ETEC vaccine.
Article
Immunology
Jun-Young Park, Seung-Hak Cho
Summary: This study constructed a peptide-specific monoclonal antibody library against heat-labile enterotoxin A subunit (LT-A) through epitope mapping, providing a basis for the development of an ETEC vaccine. Monoclonal antibodies were produced to improve the specificity of LT-A detection using synthetic peptides. The antibodies showed specificity and neutralizing effects, highlighting their importance in ETEC vaccine production and antigen identification.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Veterinary Sciences
Martin Peter Rydal, Michela Gambino, Claus Bottcher Jorgensen, Louise Ladefoged Poulsen, Lone Brondsted, Jens Peter Nielsen
Summary: Host genotype plays a crucial role in the susceptibility to enterotoxigenic E. coli (ETEC), with CHCF1 genotype possibly corresponding to susceptibility towards ETEC F4ac/ab infection.
BMC VETERINARY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Carolina Y. Garcia, Hyesuk Seo, David A. Sack, Weiping Zhang
Summary: This study demonstrates the broad immunogenicity of a multivalent ETEC subunit vaccine candidate and suggests that it can be effectively delivered via the intradermal route. The vaccine induces functional antibodies against ETEC adhesins and toxins when administered intradermally.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Mohamed Rhouma, Charlotte Braley, William Theriault, Alexandre Thibodeau, Sylvain Quessy, Philippe Fravalo
Summary: The study revealed that the ETEC:F4 strain challenge significantly affected the diversity and composition of piglet fecal microbiota, while treatment with colistin sulfate only reduced the populations of E. coli/Shigella during the treatment period.
Article
Biotechnology & Applied Microbiology
Fatemeh Mohammadkhani, Seyed Latif Mousavi Gargari, Shahram Nazarian, Maryam Mafi
Summary: This study aimed to produce IgY antibodies against CEL chimeric protein and evaluate its protective effects against ETEC. The results showed that anti-CEL IgY at a concentration of 2 mg ml(-1) could decrease bacterial adhesion to HT-29 cells by 74%, and at a concentration of 750 μg ml(-1), the IgY antibody managed to neutralize the disruptive LT toxin effect on the Y1 cell line. Passive immunotherapy with anti-CEL IgY can prevent bacterial colonization and toxicity, thus facilitating in controlling enteric diseases caused by ETEC infection.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Melibea Berzosa, Alzbeta Nemeskalova, Alba Calvo, Gemma Quincoces, Maria Collantes, Felix Pareja, Carlos Gamazo, Juan Manuel Irache
Summary: ETEC is a major pathogen causing diseases and deaths in the human population, but there is currently no licensed vaccine available. This study proposes the use of OMVs isolated from ETEC clinical strains as a vaccine, which induces an immune response in mice. Oral administration of OMVs encapsulated in nanoparticles delays their transit through the gut.
Article
Multidisciplinary Sciences
Pengpeng Xia, Siqi Lian, Yunping Wu, Li Yan
Summary: Porcine aminopeptidase N (APN) is a promising candidate for the development of mucosal epithelium-targeted vaccines. This study compared the production of APN-specific monoclonal antibodies using traditional hybridoma technology and recombinant antibody expression method. Results showed that antibodies produced in CHO cells and mAbs from hybridomas could recognize and bind to the APN protein, laying the foundation for the development of therapeutics targeting different APN-specific epitopes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biotechnology & Applied Microbiology
Pengpeng Xia, Yunping Wu, Siqi Lian, Guomei Quan, Yiting Wang, Guoqiang Zhu
Summary: The deletion of the FaeG subunit attenuates clinical symptoms and intestinal mucosal damage caused by F4ac infection, as well as reduces apoptosis in intestinal epithelial cells induced by F4ac. Targeted depletion of FaeG remains beneficial for the prevention or treatment of F4ac infection.
Article
Biotechnology & Applied Microbiology
Xuanqiang Gu, Qianxi Hou, Jiaqi Liu, Pengpeng Xia, Qiangde Duan, Guoqiang Zhu
Summary: Salmonella Enteritidis (SE) causes disease transmission in poultry industry and human food poisoning. Detection using SE-exclusive sef operon allows rapid and specific detection for epidemiological investigation and eradication of SE infections.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Pengpeng Xia, Yunping Wu, Siqi Lian, Li Yan, Xia Meng, Qiangde Duan, Guoqiang Zhu
Summary: This paper summarizes the specificity of TLRs in recognizing microbial components, discusses their role in regulating immune responses, and proposes a novel therapeutic strategy using TLRs to combat microbial infections.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Jiaqi Liu, Dong Zhang, Siqi Lian, Xuanqiang Gu, Qianxi Hou, Pengpeng Xia, Guoqiang Zhu
Summary: This study evaluated the role of NirC in an O2:K1:H7 APEC strain, finding that deletion of the nirC gene altered bacterial characteristics, possibly by inhibiting c-di-GMP synthesis in response to nitrite stress in the environment.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Da Ao, Xueliang Liu, Pengpeng Xia, Hui Wang, Sen Jiang, Wanglong Zheng, Nanhua Chen, Francois Meurens, Jianzhong Zhu
Summary: Pig TLR8 exhibits species-specific residues interacting with IQDs, different from human TLR8 counterparts. Critical residues for pig TLR8 signaling were identified, some of which differ from human TLR8 residues. The study also reveals differences in pig TLR8 response to CL075 stimulation compared to human TLR8.
MOLECULAR IMMUNOLOGY
(2021)
Article
Food Science & Technology
Peili Wang, Jiaxiang Zhang, Yanfei Chen, Haoran Zhong, Heng Wang, Jianji Li, Guoqiang Zhu, Pengpeng Xia, Luying Cui, Jun Li, Junsheng Dong, Qingqing Gao, Xia Meng
Summary: The study confirmed that clbG is essential for the synthesis of genotoxin colibactin in APEC XM strain, and its deletion reduced the likelihood of APEC meningitis in mice.
Article
Biochemistry & Molecular Biology
Siqi Lian, Jiaqi Liu, Yunping Wu, Pengpeng Xia, Guoqiang Zhu
Summary: Bacteria and viruses frequently co-infect the intestines, and one pathogen's infection can directly or indirectly affect the severity of infection by another pathogen. The presence of co-infection can lead to synergistic or antagonistic effects, which can impact the progression of the disease. This review discusses different scenarios triggered by different infection orders and summarizes the possible mechanisms of synergy or antagonism involved in co-infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Zoology
Yang Yang, Hong Zhou, Ji Shao, Xinyi Zhang, Pengpeng Xia, Mingxu Zhou, Qiangde Duan, Guoqiang Zhu
Summary: The objective of this study was to investigate the effect of the hcp1 gene on the virulence factors of F18 Shiga toxin-producing Escherichia coli. The results showed that the hcp1 gene is involved in bacterial motility, adherence, invasion, and secretion of Stx2e toxin.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Article
Immunology
Peili Wang, Jiaxiang Zhang, Yanfei Chen, Haoran Zhong, Heng Wang, Jianji Li, Guoqiang Zhu, Pengpeng Xia, Luying Cui, Jun Li, Junsheng Dong, Qingqing Gao, Xia Meng
Summary: Colibactin is synthesized by a genomic island and plays a significant role in various diseases. APEC XM strain induces meningitis in mice, and the deletion of clbH reduces colibactin production but does not affect the strain's ability to invade cells.
Review
Biology
Xia Meng, Mengping He, Pengpeng Xia, Jinqiu Wang, Heng Wang, Guoqiang Zhu
Summary: This article reviews the functions of sRNAs during Salmonella infection of host cells. sRNAs play important roles in helping Salmonella resist acidic environmental stress, regulate virulent genes for adhesion and invasion, adapt to oxidative stress within host cells, and promote survival within macrophages. By regulating gene expression, sRNAs contribute to the pathogenic mechanism of Salmonella.
Article
Microbiology
Xia Meng, Mengping He, Binjie Chen, Pengpeng Xia, Jinqiu Wang, Chunhong Zhu, Heng Wang, Guoqiang Zhu
Summary: RyhB-1 and RyhB-2 are small non-coding RNAs that regulate iron homeostasis in Salmonella by sensing iron concentration in the environment. They inhibit the growth of Salmonella Typhimurium in macrophages by modulating the expression of genes sicA and rtsB. The regulatory role of RyhBs and their targets in Salmonella Enteritidis is not well understood. We found that RyhB paralogs are expressed when Salmonella Enteritidis interacts with chicken macrophages. They downregulate survival-related virulence factors and attenuate the survival of Salmonella Enteritidis inside macrophages.
Review
Biochemistry & Molecular Biology
Pengpeng Xia, Li Yan, Xingduo Ji, Yunping Wu, Siqi Lian, Guoqiang Zhu
Summary: G protein-coupled receptor 39 (GPR39) is a zinc-sensing receptor that plays a crucial role in regulating animal intestinal health. It can sense changes in extracellular zinc ions, mediate ion transport, maintain intestinal homeostasis, and improve barrier function. GPR39 has become a potential target for studying animal intestinal diseases, providing new directions for prevention and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Veterinary Sciences
Pengpeng Xia, Siqi Lian, Yunping Wu, Li Yan, Guomei Quan, Guoqiang Zhu
Summary: Zinc plays a crucial role in the crosstalk between hosts and microbes, and imbalance in zinc concentrations may lead to changes in microbial infections. Studying the role of zinc can provide new avenues for treating microbial infections.
VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Binjie Chen, Xianchen Meng, Jie Ni, Mengping He, Yanfei Chen, Pengpeng Xia, Heng Wang, Siguo Liu, Guoqiang Zhu, Xia Meng
Summary: Research shows that two RyhB paralogs of Salmonella enterica serovar Enteritidis can upregulate Type III secretion system (T3SS) effectors, affecting the bacteria's invasion into intestinal epithelial cells, and thereby impacting its virulence.
VETERINARY RESEARCH
(2021)
