Article
Crystallography
Mitchell Paolello, Ilyes Bichari, Davinia Brouckaert, Mirvatte Francis, Dawn Yang, Gerard Capellades
Summary: This study developed a population balance model to investigate the feasibility of selectively inhibiting impurity crystallization kinetics. By using Raman spectroscopy for in-situ monitoring and calibrating a population balance model, the study achieved high purity close to equilibrium within the first two hours. The optimal approach for preventing impurity nucleation involves low impurity nucleation rate, high product seed purities, and adjustable crystallization time.
Article
Biochemical Research Methods
Laura Rolinger, Juergen Hubbuch, Matthias Ruedt
Summary: Monitoring the protein concentration and buffer composition during UF/DF step is crucial for biopharmaceutical production automation and real-time release testing. This study demonstrates the advantages of combining Raman spectroscopy with EKF for monitoring the UF/DF step, showing improved sensitivity and faster measurement speed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Mais Al-Attili, Carla Ferreira, Chris Price, Karen Faulds, Yi-Chieh Chen
Summary: Spatially offset Raman spectroscopy (SORS) is a subset of Raman spectroscopy used for probing subsurface compositions in non-homogenous media. In this study, a SORS probe was developed for the monitoring of pharmaceutical drying. The results showed that SORS could offer improved monitoring of pharmaceutical drying processes.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2023)
Review
Pharmacology & Pharmacy
Dorian Laszlo Galata, Lilla Alexandra Meszaros, Nikolett Kallai-Szabo, Edina Szabo, Hajnalka Pataki, Gyorgy Marosi, Zsombor Kristof Nagy
Summary: This paper introduces the analysis of images acquired by a digital camera with visible illumination and its applications in pharmaceutical manufacturing as a Process Analytical Technology (PAT). The development of machine vision and conversion to continuous manufacturing have accelerated the use of digital image analysis methods, enabling more comprehensive analysis and prediction of product quality attributes.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Raul S. Rangel-Gil, Nobel O. Sierra-Vega, Rodolfo J. Romanach, Rafael Mendez
Summary: This study presents the implementation of Raman spectrometer in a stream sampler for real-time monitoring of low drug concentration. Raman spectra were continuously acquired as powder blends flowed through the sampler operating at a paddle wheel speed of 10 RPM and used to develop a calibration model. The calibration model based on Partial Least Squares Regression (PLS-R) accurately quantified caffeine concentration in the range of 1.50% to 4.50% w/w. Evaluation of the model using test blends showed a root mean square error of prediction of 0.21% w/w and a low bias of -0.03% w/w. Variographic analysis demonstrated low process variance of real-time spectral acquisition. The results highlight the capability of the Raman spectrometer coupled with the stream sampler for monitoring low drug concentration in poor flowability blends.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Automation & Control Systems
Michel Thiel, Nicolas Sauwen, Tastiana Khamiakova, Tor Maes, Bernadette Govaerts
Summary: This paper introduces a practical framework based on different dimension-reduction and calibration methods for tracking chemical experiments organized in batches, aiming to visualize and interpret chemical reaction kinetics in real time or off-line, and predict chemical component concentrations over time. Results show that NMF and MCR provide better interpretability for chemical reactions compared to PCA, and unsupervised methods such as PCA, NMF or MCR can be used to predict concentrations of chemical compounds continuously with good precision and interpretability.
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
(2021)
Review
Chemistry, Analytical
A. Silge, Karina Weber, D. Cialla-May, L. Mueller-Boetticher, D. Fischer, J. Popp
Summary: The application of Raman spectroscopic methods in life science has made significant progress, driven by advances in instrumentation, miniaturization, and interdisciplinary dialogue. These advancements have brought new possibilities for optical sensing and chemical imaging, contributing to advanced pharmaceutical development and manufacturing. Raman sensing, with its non-destructive and label-free quality testing capabilities, is particularly valuable for process-related aspects in bioprocessing. Additionally, the trend towards personalized medicine has increased the demand for individually tailored medicines, manufactured in public pharmacies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Michele Panzitta, Niccolo Calamassi, Cristina Sabatini, Marzia Grassi, Chiara Spagnoli, Vittoria Vizzini, Elisa Ricchiuto, Andrea Venturini, Andrea Brogi, Judit Brassier Font, Lino Pontello, Giorgio Bruno, Paola Minghetti, Maurizio Ricci
Summary: The European Pharmacopoeia includes chapters on Raman, NIR, and Chemometrics, and there is significant academia research on these topics. However, the implementation of spectroscopic techniques in the pharmaceutical industry is still slow. Successful applications of spectroscopy in pharmaceutical production departments highlight the need for industry-academia cooperation to expedite process innovation for the benefit of patients.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Ryo Ohashi, Aria Fujii, Kanako Fukui, Tatsuo Koide, Toshiro Fukami
Summary: Transmission Raman spectroscopy was used to develop a non-destructive quantitative analytical model for the assay of a crystal dispersion-type ointment containing acyclovir. The optimized model showed good prediction performance for different label claims, indicating its usefulness in product development and quality control.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Review
Chemistry, Analytical
S. Stranzinger, D. Markl, J. G. Khinast, A. Paudel
Summary: This review discusses methods for monitoring density variations of particulate matter by describing their principles and presenting application examples, comparing these techniques in terms of measurement and data processing time, resolution and its ability to be integrated in a process. From common techniques like near-infrared spectroscopy to more advanced methods like terahertz spectroscopy, these technologies are crucial for improving efficiency and quality in pharmaceutical manufacturing.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Michael Foster, William Brooks, Philipp Jahn, Jesper Hedberg, Andreas Andersson, And Lorna Ashton
Summary: This paper introduces a new deep UV Raman instrument that can provide high specificity and accuracy for biopharmaceutical products, with the potential for online measurement.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Chemistry, Analytical
Chen Liu, Lisa Mueller-Botticher, Chang Liu, Jurgen Popp, Dagmar Fischer, Dana Cialla-May
Summary: A Raman-based label-free analytical method was developed to detect ciprofloxacin in different matrices. The method allows for semiquantification and can overcome interference from excipients and carrier substances. Surface-enhanced Raman spectroscopy is applied for sensitive detection, and the method can also monitor the degradation of ciprofloxacin within pharmaceutical formulations.
Review
Chemistry, Applied
Jaka Orehek, Dusan Teslic, Blaz Likozar
Summary: This scientific paper provides an overview of continuous solution crystallization in the pharmaceutical industry, covering topics such as comparisons between different crystallizers, solid form structuring, and online process analytical technology. It discusses mechanistic multiscale modeling, model-based predictive control, and the introduction of new continuous systems like the coiled flow inverter crystallizer. Continuous crystallization is highlighted for its efficiency, on-demand production, and potential for optimization and intensification.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Chemistry, Analytical
Danuta Liberda, Paulina Koziol, Magda K. Raczkowska, Wojciech M. Kwiatek, Tomasz P. Wrobel
Summary: The study investigated the interference effects on spectral quality of pancreatic tissues measured in transmission and transflection mode with FT-IR microscopy. The removal of paraffin altered interference magnitude in transflection, providing a platform for detailed analysis of its effect on biological material spectra. Transflection mode measurements show potential in histological classification, but require comparison with transmission mode.
Article
Pharmacology & Pharmacy
Ryo Ohashi, Tatsuo Koide, Toshiro Fukami
Summary: Transmission Raman spectroscopy (TRS) has been found to have practical prediction performance for drug content in tablets, even with variations in granulation parameters. It also shows good prediction performance for tablets manufactured at different granulation scales and thicknesses. Therefore, TRS is a versatile non-destructive quantitative analysis method that can be applied in tablet manufacturing.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Instruments & Instrumentation
Christopher Corden, Pavel Matousek, Claudia Conti, Ioan Notingher
Summary: The study investigated the use of time-gated and micro-spatially offset Raman spectroscopy for sub-surface molecular analysis in optically turbid samples. Multiplexed spectral detection allowed fast acquisition of signals for three-dimensional Raman mapping, demonstrating the potential for practical applications in various fields.
APPLIED SPECTROSCOPY
(2021)
Article
Instruments & Instrumentation
Luca Ciaffoni, Pavel Matousek, William Parker, Elin A. McCormack, Hugh Mortimer
Summary: This study presents a generic analytical model for comparing the detection performance of Raman spectrometers based on grating and spatial heterodyne designs. It shows that the SNR ratio between the two types of spectrometers can vary by two orders of magnitude, with spatial heterodyne spectrometers potentially competing with compact grating designs in delivering comparable detection performance in a much smaller footprint.
APPLIED SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Sara Mosca, Priyanka Dey, Marzieh Salimi, Benjamin Gardner, Francesca Palombo, Nick Stone, Pavel Matousek
Summary: The proposed method utilizes Spatially Offset Raman Spectroscopy (SORS) to estimate the reduced scattering coefficient (mu(s)') of turbid samples, with high accuracy achieved through calibration on a PTFE model sample. Knowledge of mu(s)' in the NIR range is valuable for optimizing SORS illumination and geometric configurations, providing in-depth information about SORS measurements, and benefiting various photon applications in fields such as biomedical, pharmaceutical, security, forensic, and cultural sciences.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sara Mosca, Priyanka Dey, Marzieh Salimi, Benjamin Gardner, Francesca Palombo, Nick Stone, Pavel Matousek
Summary: This study investigates how SORS probes for specific depths in turbid samples, highlighting the reduced scattering coefficient of the medium as a key parameter in determining the probed zone. The proposed methodology was tested with experimental data, showing its relevance to a wide range of SORS applications and the importance of providing additional information for SORS measurements.
ANALYTICAL CHEMISTRY
(2021)
Article
Physics, Multidisciplinary
A. Botteon, M. Realini, C. Colombo, C. Conti, P. Matousek, C. Castiglioni
Summary: This study systematically investigates the micro-SORS capability to characterize a system where an agent diffuses into a turbid matrix, with potential applications in heritage science and other fields. The experiments show that the slope of the Raman intensity decay rate ratio can correlate with the penetration depth of the agent, providing a non-destructive analytical tool for monitoring conservation treatments and other applications involving agent penetration.
EUROPEAN PHYSICAL JOURNAL PLUS
(2021)
Article
Chemistry, Multidisciplinary
Mehrvash Varnasseri, Howbeer Muhamadali, Yun Xu, Paul I. C. Richardson, Nick Byrd, David Ellis, Pavel Matousek, Royston Goodacre
Summary: The study explored the use of Raman spectroscopy and SORS technology for the non-destructive detection of adulteration in extra virgin olive oil. Results demonstrated that these methods have significant potential for rapid and accurate analysis.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Analytical
Alessandra Botteon, Wang-Hyo Kim, Chiara Colombo, Marco Realini, Chiara Castiglioni, Pavel Matousek, Byung-Man Kim, Tae-Hyuk Kwon, Claudia Conti
Summary: This study demonstrates the ability to monitor the dye depth profile within the light active layer of dye-sensitized solar cells using a non-destructive/non-invasive Raman method, micro-spatially offset Raman spectroscopy (micro-SORS). The results show that micro-SORS can accurately discriminate between homogeneous and inhomogeneous dye depth profiles, and its analysis is reliable compared to destructive time-of-flight secondary ion mass spectrometry measurements.
ANALYTICAL CHEMISTRY
(2022)
Article
Instruments & Instrumentation
Max Dooley, Thomas Paterson, Louise Dexter, Pavel Matousek, Hamid Dehghani, Ioan Notingher
Summary: Spatially offset Raman spectroscopy (SORS) is a powerful technique for subsurface molecular analysis of optically turbid samples. Numerical modeling and experimental validation have shown that offsetting the laser source and detector in opposite directions can significantly improve spectral contrast and signal to noise ratio.
APPLIED SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Marzieh Salimi, Sara Mosca, Benjamin Gardner, Francesca Palombo, Pavel Matousek, Nicholas Stone
Summary: The development of new effective cancer treatment methods, especially photothermal therapy using plasmonically resonant metallic nanoparticles, has garnered attention. However, the effectiveness of photothermal therapy can be limited by parameters such as pain sensation experienced by the patient. This review summarizes studies on pain management and enhancing the efficiency of photothermal therapy.
Article
Spectroscopy
Chiara Castiglioni, Alessandra Botteon, Claudia Conti, Matteo Tommasini, Pavel Matousek
Summary: This study introduces an analytical physical description of micro-spatially offset Raman spectroscopy (micro-SORS) experiments in turbid samples and highlights its use for studying the penetration depth of chemical species into a matrix. The study demonstrates the relationship between the Raman intensity ratio and the concentration profiles of the analytes using a physical model and Monte Carlo simulation. The obtained formulas provide insights for interpreting micro-SORS data and comparing different samples.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Nai-Hao Yin, Anthony W. Parker, Pavel Matousek, Helen L. Birch
Summary: The purpose of this study is to determine if Raman spectroscopy can detect age-related changes in tendon matrix molecules. The study found that old age groups had significantly higher Raman intensities and fluorescence levels compared to young tendons. Principal component analysis was able to distinguish between different age groups and tendon types. There was also a significant correlation between Raman spectra and levels of advanced glycation end-product (AGE) collagen crosslinks. These findings provide a basis for quantifying age-related chemical modifications to tendon matrix molecules in intact tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Instruments & Instrumentation
Sara Mosca, Kay Sowoidnich, Megha Mehta, William H. Skinner, Benjamin Gardner, Francesca Palombo, Nicholas Stone, Pavel Matousek
Summary: In this work, we propose an advanced CCD read-out combined with SERDS for fast acquisition rates in Raman spectroscopy. The incorporation of a periodic mask and a smaller shift of charge on the CCD allowed for a 10-fold increase in acquisition speed compared to previous designs. The 10 kHz system outperformed earlier variants in different situations, enabling accurate data acquisition from moving samples and under varying ambient light conditions. The high-speed instrument has potential applications in disease diagnosis, accurate data acquisition, and monitoring rapidly evolving Raman signals.
APPLIED SPECTROSCOPY
(2023)
Article
Instruments & Instrumentation
Kay Sowoidnich, Michael Towrie, Pavel Matousek
Summary: This paper presents a holistic approach to addressing the problems of fluorescence interference, sample heterogeneity, and laser-induced sample heating in Raman spectroscopy. Long wavelength shifted excitation Raman difference spectroscopy (SERDS) combined with wide-area illumination and sample rotation is successfully applied for the investigation of selected wood species. The results demonstrate that SERDS is effective in separating the Raman spectroscopic fingerprints of wood species from intense fluorescence interference, and sample rotation with wide-area illumination allows for representative SERDS spectra within a short time.
APPLIED SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Benjamin Gardner, Pavel Matousek, Nicholas Stone
Summary: The article introduces a method to correct self-attenuation artifacts in Raman spectroscopy in deep biological tissue analysis. The method successfully reduces Raman spectral intensity variances and distortions in specific peak ratios, with potential to improve detection limits and depth in clinical specimen analysis.
Review
Multidisciplinary Sciences
Sara Mosca, Claudia Conti, Nick Stone, Pavel Matousek
Summary: Spatially offset Raman spectroscopy (SORS) is a spectroscopic technique that allows for non-invasive chemical characterization of diffusely scattering materials. It has been widely applied in fields such as disease diagnosis, explosive detection, non-destructive analysis of pharmaceutical products and art objects, with potential future applications.
NATURE REVIEWS METHODS PRIMERS
(2021)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)