Article
Multidisciplinary Sciences
Peter Nguyen, Rony Eshaque, Barbara Anne Garland, Anthony Dang, Michael D. L. Suits
Summary: Advanced periodontitis is strongly associated with the presence of the bacterial consortia known as the red complex, which includes Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. In this study, the researchers investigated the genetic linkage and carbohydrate degradation capacity of T. forsythia, and identified a new polysaccharide utilization loci (PUL)-like operon. They also reported the crystal structures of two proteins encoded in this operon and demonstrated the enzymatic kinetics and degradation process of chondroitin sulfate A by these proteins.
Article
Multidisciplinary Sciences
Lise Lund Haheim, Dag S. Thelle, Kjersti S. Ronningen, Ingar Olsen, Per E. Schwarze
Summary: This study found that the antibody levels to the periodontitis-associated bacteria TF and TD were inversely correlated with the incidence of specific cancers, suggesting a potential association between oral infection and reduced immune response with cancer development.
Article
Microbiology
Suk Ji, Joong-Ki Kook, Soon-Nang Park, Yun Kyong Lim, Geum Hee Choi, Jae-Suk Jung
Summary: Periodontal disease is a common and costly condition that leads to tooth loss. This study analyzed the salivary microbiota to determine if specific bacterial species can distinguish the severity of periodontal disease. The findings suggest that P. gingivalis, T. forsythia, and F. alocis may serve as biomarkers for assessing the severity of periodontal disease in saliva.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Franck Zekre, Rolando Cimaz, Mireille Paul, Teresa Giani, Louis Waeckel, Anne-Emmanuelle Berger, Jean-Louis Stephan, Myriam Normand, Stephane Paul, Hubert Marotte
Summary: This study found that children with oligoarticular and ERA subsets in juvenile idiopathic arthritis (JIA) had higher IgG titers against P. gingivalis and P. intermedia, indicating the involvement of oral dysbiosis in the occurrence of JIA in these subgroups.
Article
Immunology
Anna Svard, Alf Kastbom, Karin Roos Ljungberg, Barbara Potempa, Jan Potempa, G. Rutger Persson, Stefan Renvert, Johan Sanmartin Berglund, Maria K. Soderlin
Summary: A Swedish study found that patients with rheumatoid arthritis (RA) had higher levels of saliva antibodies to Porphyromonas gingivalis (P. gingivalis) and these antibodies were associated with RA disease activity, but not with periodontitis or serum antibodies to citrullinated proteins (ACPA).
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Gina R. Lewin, Kendall S. Stocke, Richard J. Lamont, Marvin Whiteley
Summary: This study characterizes the behavior and virulence of the periodontal pathogen Porphyromonas gingivalis during human infection. It reveals that P. gingivalis is highly expressed in diseased samples, with genes related to virulence factors and growth/matabolism being actively expressed. The study also shows that the gene expression of P. gingivalis during periodontitis is highly conserved with logarithmic laboratory growth, suggesting that simple test tube growth can be used as a model for studying P. gingivalis biology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Seonhwa Park, Kiryeon Park, Hee Sam Na, Jin Chung, Haesik Yang
Summary: An electrochemical biosensor for sensitive detection of Porphyromonas gingivalis in saliva was developed without the need for washing and separation steps. This biosensor can be used as an initial diagnosis method to distinguish between healthy people and patients with periodontal diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Rheumatology
Joyce A. Lee, Ted R. Mikuls, Kevin D. Deane, Harlan R. Sayles, Geoffrey M. Thiele, Jess D. Edison, Brandie D. Wagner, Marie L. Feser, Laura K. Moss, Lindsay B. Kelmenson, William H. Robinson, Jeffrey B. Payne
Summary: The objective of this study was to quantify the association between anti-P. gingivalis serum antibody concentrations and the risk of developing rheumatoid arthritis (RA), and to quantify the associations among RA cases between anti-P. gingivalis serum antibody concentrations and RA-specific autoantibodies. The results showed no longitudinal elevations of anti-bacterial serum antibody concentrations in RA patients prior to RA diagnosis compared to controls, but anti-P. intermedia displayed significant associations with RA autoantibody concentrations prior to RA diagnosis.
SEMINARS IN ARTHRITIS AND RHEUMATISM
(2023)
Article
Biotechnology & Applied Microbiology
Rui Li, Mengjie Hou, Liying Yu, Wen Luo, Jie Kong, Renmei Yu, Ruihan Liu, Qian Li, Lisi Tan, Chunling Pan, Hongyan Wang
Summary: This study aimed to investigate the effects of salivary histatin 5 (Hst5) on Porphyromonas gingivalis (P. gingivalis) biofilms in vitro and in vivo and the possible mechanisms. The results showed that 25μg/mL Hst5 effectively inhibited biofilm formation and increased concentrations of Hst5 increased the inhibitive effect. Hst5 could regulate membrane function and metabolic processes in P. gingivalis, and RpoD and FeoB proteins might play important roles in this process. Moreover, 100μg/mL Hst5 inhibited periodontal inflammation and alveolar bone loss in rat periodontitis through its antibacterial and anti-inflammatory effects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Immunology
Banndith Cheat, Coralie Torrens, Asmaa Foda, Brigitte Baroukh, Jeremy Sadoine, Lotfi Slimani, Veronique Witko-Sarsat, Olivier Huck, Marjolaine Gosset, Jerome Bouchet
Summary: Our study investigates the role of NLRP3 in periodontitis, demonstrating its ambiguous role in neutrophils and its interaction with Porphyromonas gingivalis. The study reveals that NLRP3 is involved in regulatory pathways that limit periodontitis in mouse models, but its protective effect is diminished in the presence of P. gingivalis. Overexpression of NLRP3 in connective tissue leads to increased production of mature IL-1 beta and mobilization of neutrophils, while NLRP3 deficiency results in lower IL-1 beta expression and almost no neutrophil mobilization.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ayub Hussein Rashid, Sarhang Sarwat Gul, Hoshyar Abdullah Azeez, Shokhan Hamaali Azeez
Summary: This study investigated the antibacterial and antibiofilm activities of Cuminum cyminum (CEO) and Foeniculum vulgare (FEO) essential oils on clinically isolated Porphyromonas gingivalis (P. gingivalis) and Prevotella intermedia (P. intermedia). Both CEO and FEO exhibited inhibition zones against the clinical strains, with MIC values of 3.125 & mu;L/mL and MBC of 6.25 & mu;L/mL against P. gingivalis, and MIC values of 0.195 & mu;L/mL and MBC of 3.125 & mu;L/mL against P. intermedia. The essential oils also showed moderate to strong antibiofilm activities against these bacteria. This study supports the use of CEO and FEO as adjuncts to periodontal therapy.
APPLIED SCIENCES-BASEL
(2023)
Article
Immunology
Xiaomiao Fan, Shaowen Zheng, Chen Chen, Li Lin, Hongyan Wang, Yuqin Shen, Yaping Pan, Chen Li
Summary: This study investigated the role of sialidase in immune evasion of P. gingivalis by examining its effects on macrophage polarization, antigen presentation, and phagocytosis. The results showed that Delta PG0352 strain, lacking the sialidase gene, was more easily cleared by macrophages and exhibited increased M1 polarization, antigen presentation, and phagocytosis compared to the wild-type strain. This suggests that sialidase plays an important role in P. gingivalis immune evasion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Shuhui Zhou, Yaoting Ji, Hantao Yao, Haiying Guo, Zichen Zhang, Zijun Wang, Minquan Du
Summary: This study found that ginsenoside Rd has inhibitory effects on periodontitis-related bacteria and inflammatory response, as well as the ability to inhibit bone resorption induced by periodontitis. The results provide potential evidence for the development of Rd as a medication for the control and prevention of periodontitis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Younggap Lim, Hyun Young Kim, Dohyun Han, Bong-Kyu Choi
Summary: This study analyzed the proteome and inflammatory response of extracellular vesicles (EVs) released from macrophages infected with the periodontal pathogen Tannerella forsythia. The results showed that T. forsythia infection enriched macrophage-derived EVs with pro-inflammatory cytokines and inflammatory mediators associated with periodontitis progression, while T. forsythia-derived vesicles carried various virulence factors and induced pro-inflammatory responses.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Marta Kaminska, Malgorzata Benedyk-Machaczka, Karina Adamowicz, Ardita Aliko, Kamila Drzazga, Klaudia Slysz, Ewa Bielecka, Jan Potempa, Piotr Mydel
Summary: This study evaluated the antimicrobial effects of bestatin, a potential drug candidate for chronic periodontitis (CP). The results showed that bestatin exhibited bacteriostatic activity against periodontopathogens and controlled the formation and composition of the biofilm. Additionally, bestatin promoted the phagocytosis of periodontopathogens by immune cells and reduced inflammation. These findings suggest that bestatin is a promising choice for the treatment and prevention of periodontitis.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Obstetrics & Gynecology
Ozlem Naciye Sahin, Aysel Ozpinar, Muhittin Serdar
Summary: The study evaluated levels of MMP-1, TIMP-1, leptin, IL-6, and fatty acids in breastmilk from women who consumed omega-3. It found that MMP-1 levels were significantly lower in breastmilk from omega users, and positively correlated with omega 6:3 ratio, IL-6, and leptin levels. Maternal omega use may modify MMP-1 levels in breastmilk, impacting extracellular matrix remodelling and metabolic programming in early infancy.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Meeting Abstract
Rheumatology
A. Kor, S. Erten, E. F. Yurt, I. Dogan, H. Apaydin, M. A. Serdar, C. Bicer
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Medical Laboratory Technology
Hakan Apaydin, Cemile Koca Bicer, Emine Feyza Yurt, Muhittin Abdulkadir Serdar, Ismail Dogan, Sukran Erten
Summary: This study aimed to investigate the plasma levels of tryptophan (Trp) and its metabolites in patients with primary Sjogren's syndrome (pSS). The study included 34 pSS patients and 42 healthy individuals, and serum Trp and kynurenine (Kyn) concentrations were measured. The results showed that Trp metabolism was altered in pSS patients, suggesting a possible link between Trp metabolism and the pathogenesis of pSS.
LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Adnan Hasimi, Oezlem Dogan, Ceyhan Ceran Serdar, Muhittin A. Serdar
Summary: This study compares the concentrations of arginine and methylated arginine products in serum samples of COVID-19 patients to assess their association with disease severity. The results show that the concentrations of ADMA, SDMA, and L-NMMA in severe patients are significantly higher than in mild-moderate patients and the control group. Additionally, L-NMMA, CRP, and NLR are identified as independent risk factors for severe COVID-19 patients.
Article
Physics, Atomic, Molecular & Chemical
Zelal Zuhal Kaya, Muhittin Serdar, Fehime Aksungar, Meltem Kilercik, Mustafa Serteser, Ahmet Tarik Baykal
Summary: A novel method for measuring serum free light chains (FLCs) using immunoenrichment on micro-affinity chromatography in combination with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) detection was developed. The results showed good agreement between the proposed method and the FLC assay, making it a valuable tool for screening, prognostic stratification, and monitoring therapy responses in plasma cell disorders (PCDs).
EUROPEAN JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Zeliha Guney, Sivge Kurgan, Canan Onder, Cavid Mammadov, Muhittin A. Serdar, Meral Gunhan
Summary: This study aimed to evaluate the levels of ADMA, SDMA, and IL-1 beta in GCF from periodontitis patients. The results showed that the levels of IL-1 beta, ADMA, and SDMA in GCF were significantly higher in periodontitis patients compared to the control group, and they were positively correlated with clinical periodontal parameters. It suggests that ADMA and SDMA may be involved in the pathogenesis of periodontal disease.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Melis Yilmaz, Ekin Yay, Nur Atalay, Nur Balci, Sivge Kurgan, Hilal Toygar, Muhittin A. A. Serdar
Summary: This study aimed to determine the levels of arginine metabolites and IL-6 in saliva and serum of individuals with periodontitis and healthy individuals, and to evaluate their association with smoking status. The results showed that salivary concentrations of ADMA and SDMA were associated with periodontitis, and smoking increased the levels of arginine metabolites in the serum of periodontitis patients.
Article
Dentistry, Oral Surgery & Medicine
V. Umut Bengi, Erkan oezcan, N. Isil Saygun, O. Sebnem Guler, Muhittin A. Serdar
Summary: This study aimed to evaluate the levels of adipokines in the gingival crevicular fluid (GCF) of periodontitis patients and compare them before and after non-surgical periodontal treatment. The results showed that the levels of visfatin and chemerin were significantly higher in the periodontitis group compared to the healthy group. After non-surgical periodontal treatment, the levels of chemerin significantly decreased. These findings suggest that visfatin and chemerin may play a role in the periodontal disease process.
Article
Biochemistry & Molecular Biology
Emine Feyza Yurt, Cemile Bicer, Muhittin A. Serdar, Selsuk Akan, Sukran Erten
Summary: Various studies have shown that the Kyn pathway is important in regulating the immune system. In this study, it was found that the Kyn pathway is accelerated and proinflammatory cytokine levels are decreased in patients with axSpA.
Article
Dentistry, Oral Surgery & Medicine
Canan Onder, Nihan Akdogan, Sivge Kurgan, Nur Balci, Ceyhan Ceran Serdar, Muhittin A. Serdar, Meral Gunhan
Summary: The aim of this study was to investigate the effects of smoking and periodontal inflammation on tryptophan-kynurenine metabolism and their correlation with clinical periodontal parameters. The study found that smoking exacerbates inflammation in the periodontium, leading to increased tryptophan destruction and decreased IDO activity by suppressing the kynurenine pathway (KP) in serum. Therefore, kynurenine and its metabolites may serve as significant biomarkers connecting smoking and periodontal disease.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Duygu Tas, Sivge Kurgan, Zeliha Guney, Muhittin A. Serdar, Dimitris N. Tatakis
Summary: This study aimed to determine the effects of smoking on early clinical outcomes and molecular biomarkers following root coverage surgery. The results showed no significant differences between smokers and nonsmokers in clinical parameters and molecular markers before and after surgery. Based on the findings, the effectiveness of root coverage surgery is similar in smokers and nonsmokers.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Infectious Diseases
Neval Yurttutan Uyar, Ahmed Kerem Sayar, Ayse Sesin Kocagoez, Muhittin Abduelkadir Serdar, Rehile Zengin, Zeynep Tugce Sarikaya, Meltem Kilercik, Veysel Balci, Mustafa Serteser
Summary: This study compared the diagnostic values of individual and composite biomarkers in predicting bacteremia in adults. The results showed that combined biomarkers, especially combined 2, had the best predictive performance and could be used as a diagnostic tool for bacteremia before culture results are available.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2023)
Article
Biochemistry & Molecular Biology
Esra Ayan, Hasan Demirci, Muhittin Abdulkadir Serdar, Francesca Palermo, Ahmet Tarik Baykal
Summary: This study aims to reveal the role of gut microbiota in the pathogenesis of Alzheimer's Disease (AD), particularly regarding changes in the proteome and molecular pathways. The data indicates that the microbiota, proteome, and molecular changes exist long before the manifestation of disease symptoms. Moreover, disparities exist between the normal-aged flora and the gut microbiota of late-stage AD mice, emphasizing the potential value of identified phyla, proteins, and pathways as markers for the early and late stages of AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Neval Yurttutan Uyar, Arda Ahmet, Muhittin Serdar, Sesin Kocagoz, Meltem Kilercik, Mustafa Serteser
Summary: This study aimed to evaluate the diagnostic accuracy of SARS-CoV-2 antibody tests in current and past infections, determine the proper testing time and cutoff values. The results showed that IgG and total antibody tests had better performance compared to other antibody types, especially after 10 days of symptom onset. These findings are important for the diagnosis and monitoring of COVID-19.
NEW MICROBIOLOGICA
(2022)
Article
Biochemistry & Molecular Biology
Mehem Kilercik, Ihsan Yozgat, Muhittin A. Serdar, Fehime Aksungar, Sema Gogus, Semra Solak, Zelal Zuhal Kaya, Ali Murat Yayla, Mustafa Serteser
Summary: This study used multiple regression analysis techniques to evaluate the effect size of each parameter in first trimester Down Syndrome risk analysis. The results showed that PAPP-A levels, NT levels, DS story, and hCG beta levels affect the first trimester risk analysis results.
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
(2022)