Article
Surgery
Yuto Hozaka, Hideyuki Oi, Souichi Satake, Yoshinori Uchino, Yuichi Goto, Tetsuya Idichi, Kiyonori Tanoue, Yoichi Yamasaki, Yota Kawasaki, Yuko Mataki, Hiroshi Kurahara, Akihiro Nakajo, Michiyo Higashi, Akihide Tanimoto, Tsuyoshi Sugiura, Takao Ohtsuka
Summary: This study investigated the association between specific microbiota and the clinicopathologic characteristics of intraductal papillary mucinous neoplasms of the pancreas. They found that the relative abundance of Firmicutes was lower and Proteobacteria and Fusobacteria were higher in the main duct operational taxonomic units compared to branch duct operational taxonomic units. Invasive intraductal papillary mucinous neoplasms had significantly higher relative abundances of Bacteroidetes and Fusobacteria. The relative abundance of the intestinal type was significantly lower in Firmicutes compared to the nonintestinal type.
Article
Oncology
Xuehua Kong, Yu Zhang, Linwei Xiang, Yan You, Yaqian Duan, Yuqing Zhao, Shue Li, Rui Wu, Jiangbo Zhang, Lan Zhou, Liang Duan
Summary: This study investigated the role of neutrophil extracellular traps (NETs) in Fusobacterium nucleatum (Fn)-related colorectal carcinoma (CRC) and its potential clinical significance. The results showed that Fn infection led to increased NETs production and promoted CRC progression through angiogenesis and metastasis. The combination of circulating NETs and carcinoembryonic antigen (CEA) could be used as a novel screening strategy for predicting CRC occurrence and metastasis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Microbiology
Caitlin A. Brennan, Geicho Nakatsu, Carey Ann Gallini Comeau, David A. Drew, Jonathan N. Glickman, Robert E. Schoen, Andrew T. Chan, Wendy S. Garrett
Summary: The study demonstrates that aspirin has specific antibacterial effects on Fusobacterium nucleatum, a bacterium associated with colorectal cancer. Individuals who use aspirin daily have lower levels of this bacterium in colon adenoma tissues. This suggests the potential for aspirin to modulate bacteria in vivo for improved colorectal cancer outcomes.
Article
Biochemistry & Molecular Biology
Shanru He, Xue Bai, Yongbin Xu
Summary: In this study, the structure of Fn0495, a thiolase in F. nucleatum, was analyzed to gain insights into its molecular mechanism. Fn0495 showed unique differences in the charge and structure of the substrate binding pocket compared to homologous proteins. Three conserved residues in Fn0495 were found near a potential substrate binding pocket. These findings contribute to our understanding of the molecular characteristics and roles of the thiolase family.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Jiali Chen, Pai Zhang, Yan Zhao, Jie Zhao, Xiaobo Wu, Ruijia Zhang, Ruitao Cha, Qingxin Yao, Yuan Gao
Summary: This study developed a nitroreductase-instructed supramolecular self-assembly system to enhance the sensitivity of colorectal cancer cells to chemotherapy drugs by agglutinating and eliminating F. nucleatum bacteria. The local introduction of these assemblies efficiently inhibited tumor growth in mice bearing F. nucleatum-infected colorectal cancer. This study broadens the application range of enzyme-instructed supramolecular self-assembly and provides a new approach to treat CRC.
Article
Multidisciplinary Sciences
Kaiyuan Zhu, Alejandro A. Schaeffer, Welles Robinson, Junyan Xu, Eytan Ruppin, A. Funda Ergun, Yuzhen Ye, S. Cenk Sahinalp
Summary: The authors develop CAMMiQ, a combinatorial optimization approach that utilizes variable length, unique and doubly-unique genomic segments, showing improved identification and quantification of distinct microbes in metagenomic sequence data.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Shu-chen Huangfu, Wen-bin Zhang, Hao-ran Zhang, Yang Li, Yi-ran Zhang, Jin-lin Nie, Xiao-dong Chu, Chang-shun Chen, Hai-ping Jiang, Jing-hua Pan
Summary: This study revealed that high levels of F. nucleatum are associated with poor prognosis in CRC patients, as well as with tumor growth, distant metastasis, poor differentiation, MSI-high, and KRAS mutation.
Letter
Immunology
Louis Wolff, Delphine Martiny, Veronique Yvette Miendje Deyi, Evelyne Maillart, Philippe Clevenbergh, Nicolas Dauby
Summary: This study reports 4 cases of Fusobacterium nucleatum bacteremia associated with COVID-19, 3 of which occurred simultaneously with COVID-19 diagnosis and 1 occurred on day 15 of intensive care. None of the patients had known risk factors for F. nucleatum bacteremia, suggesting that F. nucleatum infection could be a possible complication of COVID-19.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Immunology
Lijun Hu, Yan Liu, Xuehua Kong, Rui Wu, Qi Peng, Yan Zhang, Lan Zhou, Liang Duan
Summary: The research found that Fusobacterium nucleatum infection is closely associated with the development of colorectal cancer, primarily by promoting the polarization of M2 macrophages to exacerbate tumor malignancy. S100A9 is a key signaling molecule that regulates the activation of M2 macrophages. Targeting the TLR4/NF-kappa B/S100A9 cascade may serve as a promising therapeutic strategy for Fusobacterium nucleatum-associated colorectal cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Haibo Chang, Rajeev Mishra, Chaoping Cen, Yunqing Tang, Chenchen Ma, Sanjeev Wasti, Yuanyuan Wang, Qianying Ou, Kaining Chen, Jiachao Zhang
Summary: This study analyzed the gut microbiota of CRC patients in Hainan and identified region-specific bacterial and functional biomarkers, which could potentially serve as non-invasive markers for CRC detection. Further research on gut microbiota in different geographic regions, especially in the diverse population of China, is needed to expand the understanding of markers for CRC.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Felix Ellett, Nazli I. Kacamak, Carla R. R. Alvarez, Eduardo H. S. Oliveira, Hatice Hasturk, Bruce J. J. Paster, Alpdogan Kantarci, Daniel Irimia
Summary: Recent studies have found that Fusobacterium nucleatum (Fn), a common bacterium in the oral cavity, is associated with various systemic diseases. The mechanisms behind this association are still unclear. In this study, we use advanced technology to investigate the interactions between Fn and neutrophils. Our findings show that Fn can survive within human neutrophils and be transported over long distances by these cells. We validate these observations in vivo using a zebrafish model. These results support the hypothesis that bacterial dissemination by neutrophils may be a link between oral and systemic diseases, and could potentially lead to new therapeutic approaches.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tian Zhao, Jiaqi Chen, Shuai Liu, Jie Yang, Juan Wu, Leiying Miao, Weibin Sun
Summary: Periodontitis is a chronic infectious disease that can lead to various dental problems and even tooth loss. The dental plaque biofilm is a crucial factor in the development of periodontitis. Fusobacterium nucleatum plays an important role in the structure and ecology of dental plaque biofilms. Understanding the molecular mechanism of F. nucleatum during biofilm development is essential for controlling periodontitis. This study used RNA-sequencing and quantitative reverse transcriptase PCR (RT-qPCR) to analyze the gene expression profiles of F. nucleatum in the planktonic and biofilm phases. The results revealed significant differences in gene expression between the two states, with several genes related to metabolism, cell growth, division, oxidative stress, virulence, and oral malodor being differentially expressed. Additionally, the study identified lysine as a potential antibacterial and antibiofilm agent against F. nucleatum.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Lijuan Liu, Shengting Zhang, Xiaodan Zheng, Hongmei Li, Qi Chen, Kunhao Qin, Yafang Ding, Yunlin Wei
Summary: Intracellular Fe3+ plays a critical role in human health, and the development of efficient probes for its detection is crucial for early disease diagnosis. Carbon dots synthesized from Fusobacterium nucleatum demonstrate strong fluorescence, stability, and biocompatibility, with promising applications for intracellular Fe3+ detection in vivo.
ANALYTICAL METHODS
(2021)
Article
Microbiology
Cody A. Despins, Scott D. Brown, Avery Robinson, Andrew J. Mungall, Emma Allen-Vercoe, Robert A. Holt
Summary: The study found that F. nucleatum-induced host cell transcriptional modulation involves strong upregulation of genes related to immune migration and inflammatory processes, in addition to identifying genes strongly upregulated in a cell line-specific manner. Furthermore, extensive host cell epigenomic changes specific to host cell type were observed upon F. nucleatum exposure.
Article
Cell Biology
Jessie Qiaoyi Liang, Yao Zeng, Effie Yin Tung Lau, Yuting Sun, Yao Huang, Tingyu Zhou, Zhilu Xu, Jun Yu, Siew Chien Ng, Francis Ka Leung Chan
Summary: A newly developed probiotic formula was found to modulate the dysbiosis of gut microbiota associated with colorectal cancer by inhibiting the growth of pathogenic bacteria.
Article
Dentistry, Oral Surgery & Medicine
Z. Yonel, T. Kocher, I. L. C. Chapple, T. Dietrich, H. Voelzke, M. Nauck, G. Collins, L. J. Gray, B. Holtfreter
Summary: The study aims to develop and validate a score in dental settings to identify individuals at risk of undiagnosed nondiabetic hyperglycemia or type 2 diabetes. The model includes several variables such as age, sex, body mass index, smoking status, and dental factors. The predictive performance and clinical utility of the model were assessed.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Paola de Pablo, Stefan Serban, Isabel Lopez-Oliva, Joanna Rooney, Kirsty Hill, Karim Raza, Andrew Filer, Iain Chapple, Thomas Dietrich
Summary: This study investigated the impact of periodontal treatment on disease activity in patients with active rheumatoid arthritis (RA) through a randomized controlled trial. The results showed that periodontal therapy can significantly improve periodontal disease outcomes and overall RA disease activity, although complete resolution of periodontal inflammation was difficult to achieve in some cases.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Praveen Sharma, Caroline Kristunas, Iain L. Chapple, Thomas Dietrich
Summary: This study investigates the associations between periodontal health and patient-reported outcomes in a large non-specialist dental practice patient cohort, considering changes over time. The results show that worsening periodontal health is associated with increased odds of oral pain/discomfort, dietary restrictions, and poor dental appearance.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michaela Kearney, Paul R. Cooper, Anthony J. Smith, Henry F. Duncan
Summary: Exciting opportunities exist within regenerative endodontics for the development of targeted biomaterials that harness epigenetic machinery to control pulpitis and stimulate repair. In this study, small RNA sequencing and bioinformatic analysis were used to establish a miRNA expression profile for mineralising dental pulp cells (DPCs) and examine their interaction with histone deacetylase inhibitors and DNA methyltransferase inhibitors. The results showed that these inhibitors induced mineralisation but reduced cell growth, suggesting an increased and dynamic interaction between miRNA and epigenetic modifiers during DPC reparative processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
David Herrera, Tord Berglundh, Frank Schwarz, Iain Chapple, Soren Jepsen, Anton Sculean, Moritz Kebschull, Panos N. Papapanou, Maurizio S. Tonetti, Mariano Sanz
Summary: This study aims to develop clinical practice guidelines for the prevention and treatment of peri-implant diseases, emphasizing the need for interdisciplinary approaches and interventions at different stages. The guidelines provide recommendations for maintaining healthy peri-implant tissues and managing peri-implant diseases.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Melissa M. M. Grant, Ann E. E. Scott, John B. B. Matthews, Helen R. R. Griffiths, Iain L. C. Chapple
Summary: Curcumin can decrease inflammatory response induced by oral bacterium and be potentially useful in treating gingivitis. Its mechanism of action involves the modulation of transcription factors and enzymes related to the inflammatory cascade.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Correction
Dentistry, Oral Surgery & Medicine
Rabia Majeed, Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayati Azami, Noor Hayaty Abu Kasim, Mohamed Shady Nabhan, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed
Review
Dentistry, Oral Surgery & Medicine
Rabia Majeed, Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayati Azami, Noor Hayaty Abu Kasim, Mohamed Shady Nabhan, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed
Summary: This systematic review examined the effects of nano-sized cement particles on the properties of calcium silicate-based cements. A literature search was conducted and 17 eligible studies were included. The results showed that nano-calcium silicate-based cements have better physical, mechanical, and biological properties compared to commonly used cements. However, there were deficiencies in characterizing and verifying the nano-particle size, and the presence of additives may have influenced the properties.
Review
Dentistry, Oral Surgery & Medicine
Iain L. C. Chapple, Josefine Hirschfeld, Alpdogan Kantarci, Asaf Wilensky, Lior Shapira
Summary: Neutrophils, also called granulocytes, are myeloid cells that play a critical role in acute and chronic inflammation, as well as wound healing. They express a variety of surface receptors to facilitate their migration, recognition, and destruction of pathogens or damaged tissues. Neutrophils can undergo different forms of programmed cell death and exhibit heterogeneous behavior and biological repertoire.
PERIODONTOLOGY 2000
(2023)
Review
Dentistry, Oral Surgery & Medicine
Eisha Imran, Paul R. Cooper, Jithendra Ratnayake, Manikandan Ekambaram, May Lei Mei
Summary: This article provides a contemporary review of the potential beneficial effects of nanosized hydroxyapatite (nHAP) on caries lesions demonstrated in in vitro studies. Data showed that nHAP has the potential to promote mineralization in initial caries and has antimicrobial effects through metal substitution. Metal-substituted fluorhydroxyapatite nanoparticles can potentially possess dual action property and enhanced chemical stability and bioactivity, providing a promising synergistic strategy for the development of dental therapeutics.
Article
Dentistry, Oral Surgery & Medicine
Beant Singh Thandi, Rosemarie Jane, Vinay Chavda, Iain L. C. Chapple
Summary: This case report describes the successful diagnosis and management of a rare condition called non-ulcerating necrotising sialometaplasia, which was confounded by a simultaneous episode of pericoronitis. The importance of investigating suspicious lesions and listening to patients' complaints is emphasized.
BRITISH DENTAL JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Satnam S. Virdee, Nasir Z. Bashir, Milan Krstic, Josette Camilleri, Melissa M. Grant, Paul R. Cooper, Phillip L. Tomson
Summary: This study identified optimized parameters for periapical tissue fluid (PTF) paper point sampling and compared the proteomes of PTF from teeth with normal apical tissues (NAT) and asymptomatic apical periodontitis (AAP) using high-throughput proteomic analysis. The results showed that some proteins were closely associated with AAP and had good diagnostic performance. The findings provide a potential biomarker network for clinical applications and further validation is needed.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Infectious Diseases
Sian E. Faustini, Alex Cook, Harriet Hill, Saly Al-Taei, Jennifer Heaney, Elena Efstathiou, Chloe Tanner, Neal Townsend, Zahra Ahmed, Mohammad Dinally, Madeeha Hoque, Margaret Goodall, Zania Stamataki, Timothy Plant, Iain Chapple, Pitch Consortium, Adrian M. Shields, Alex G. Richter
Summary: This study investigated the importance of salivary SARS-CoV-2 antibodies and found that salivary antibody responses differ from serum antibody responses, typically lacking neutralizing activity. While saliva is an attractive non-invasive surveillance tool, it cannot be used as a proxy for serum antibody measurement.
JOURNAL OF INFECTION
(2023)
Review
Dentistry, Oral Surgery & Medicine
P. A. Miguez, E. Bash, M. L. Musskopf, S. A. Tuin, A. Rivera-Concepcion, I. L. C. Chapple, J. Liu
Summary: Proteoglycans are core proteins associated with highly variable carbohydrate/sugar moieties. These carbohydrates, named glycosaminoglycans, play multiple roles in inflammation, immunity, and tissue structure. Heparan sulfate, a highly sulfated polysaccharide, has been found to be involved in regulating interactions between damage associated molecular patterns and inflammatory receptors. Recent advancements in producing structurally defined heparan sulfate have provided new insights into its potential therapeutic applications for periodontitis and bone formation.
PERIODONTOLOGY 2000
(2023)
Article
Engineering, Biomedical
Thomas E. Robinson, Mathieu Y. Brunet, Iain Chapple, Adrian H. M. Heagerty, Anthony D. Metcalfe, Liam M. Grover
Summary: A novel approach using low-cost polysaccharide iota carrageenan is effective at preventing fibrotic diseases and improving quality of life. It inhibits collagen fibril formation, abrogates the TGF-beta 1 pathway, and provides lubrication. The study demonstrates the potential of self-delivering immunomodulatory polysaccharides as a safe and cost-effective platform for clinical benefit.
ADVANCED NANOBIOMED RESEARCH
(2023)
