Article
Multidisciplinary Sciences
Martijn G. H. Sanders, Tamar Nijsten, Joost Verlouw, Robert Kraaij, Luba M. Pardo
Summary: The study found differences in the microbiome composition between patients with seborrheic dermatitis (SD) and individuals without diseases, mainly in the genus Staphylococcus.
Article
Microbiology
Laure Flurin, Matthew J. Wolf, Cody R. Fisher, Edison J. Cano Cevallos, James J. Vaillant, Bobbi S. Pritt, Daniel C. DeSimone, Robin Patel
Summary: This prospective pilot study evaluated the use of targeted metagenomic sequencing (tMGS) for early pathogen detection and identification in infective endocarditis (IE). The results indicate that blood-based tMGS may provide pathogen identification in subjects with IE, including culture-negative cases.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Food Science & Technology
Devin A. A. Bowes, Erin M. M. Driver, Sonja Savic, Qiwen Cheng, Corrie M. M. Whisner, Rosa Krajmalnik-Brown, Rolf U. U. Halden
Summary: This study demonstrates that wastewater-based epidemiology can be used for near real-time population dietary assessments, providing a promising tool for actionable and cost-effective data collection to support public health nutrition at the population scale.
Article
Integrative & Complementary Medicine
Yanan Wang, Li Yuan, Li Wang, Zhiyuan Xu, Hua Ruan, Xiangdong Cheng, Changwu Dong
Summary: This study explored the correlation between tongue and oral microbiota in patients with chronic gastritis. The microbial community structure of different tongue coating types was analyzed using 16S rDNA gene sequencing. Significant differences were found in the microbial diversity and abundance of tongue fur between patients with chronic gastritis and healthy controls. The dominant bacteria identified included Bacteroidetes, Proteobacteria, and Firmicutes.
JOURNAL OF TRADITIONAL CHINESE MEDICINE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Rodrigo Rodrigues Amaral, Tiago Braga, Jose F. Siqueira Jr, Isabela N. Rocas, Caio Tavora Coelho da Costa Rachid, Anna Gabriella Guimaraes Oliveira, Maria Ilma de Souza Cortes, Robert Mattew Love
Summary: This study used high-throughput sequencing technology to analyze the endodontic microbiome of teeth with asymptomatic apical periodontitis. The results revealed a high bacterial diversity and species composition variability among root canal samples. Large lesions showed a higher number of species but did not significantly differ from small lesions in bacterial diversity.
JOURNAL OF ENDODONTICS
(2022)
Article
Infectious Diseases
Shuai Wang, Peng Wang, Jun Liu, Chunxia Yang, Tianmeng Li, Jingxian Yang, Li Gu, Ming Wei
Summary: A new TaqMan real-time PCR assay was developed for rapidly detecting Nocardia spp. in respiratory samples. The assay exhibited high specificity, sensitivity, repeatability, and reproducibility, and yielded results within 3 hours of sample processing. It is expected to reduce the time required for diagnosing and treating nocardiosis.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Hubiao Jiang, Luqiong Lv, Temoor Ahmed, Shaomin Jin, Muhammad Shahid, Muhammad Noman, Hosam-Eldin Hussein Osman, Yanli Wang, Guochang Sun, Xuqing Li, Bin Li
Summary: This study found that metal oxide nanoparticles can reduce the incidence of tomato bacterial wilt and alter the composition and structure of the rhizosphere bacterial community. Among them, the application of CuO nanoparticles significantly affected the abundance of beneficial Streptomyces bacteria. This research provides evidence and strategies for using metal oxide nanoparticles to prevent and control soil-borne disease tomato bacterial wilt.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Laure Flurin, Matthew J. Wolf, Melissa M. Mutchler, Matthew L. Daniels, Nancy L. Wengenack, Robin Patel
Summary: This study evaluated the addition of next-generation sequencing (NGS) to Sanger sequencing-based 16S rRNA gene PCR assay for diagnosing bacterial infection. The results showed that adding NGS substantially improved test positivity compared to Sanger sequencing alone. The assay incorporating NGS was more sensitive than culture, especially in patients who had received antibiotic therapy.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Immunology
Laure Flurin, Matthew J. Wolf, Melissa M. Mutchler, Matthew L. Daniels, Nancy L. Wengenack, Robin Patel
Summary: The addition of NGS to the Sanger sequencing-based 16S rRNA gene PCR assay significantly improved test positivity. The clinical sensitivity of the combined assay was higher than traditional culture, especially in patients who had received antibiotic therapy.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Yeon Bee Kim, Tae Woong Whon, Joon Yong Kim, Juseok Kim, Yujin Kim, Se Hee Lee, Seong-Eun Park, Eun-Ju Kim, Hong-Seok Son, Seong Woon Roh
Summary: Although little is known about the urogenital archaeome, several reports have indicated its association with health in other body sites. This study aimed to determine the presence and diversity of archaeomes in the human urogenital tract. Analysis of urine samples from 373 asymptomatic individuals revealed the presence of diverse archaea, including Methanobacteriota, Thermoproteota, and Halobacteriota. The urogenital archaeome showed partial taxonomic compositional similarities with archaeomes from other body sites. These findings provide new insights into the role of archaea in urinary tract health.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Massa Saab, Denis Mestivier, Masoudreza Sohrabi, Christophe Rodriguez, Mahmood Reza Khonsari, Amirhossein Faraji, Iradj Sobhani
Summary: The study found a significant difference in biliary microbiota between extrahepatic cholangiocarcinoma (CCA) patients and controls without associated diseases, indicating a specific CCA-related dysbiosis. This suggests that a microbial community may be involved in the pathogenesis of CCA.
Article
Chemistry, Analytical
Xu Chen, Yue Ma, Shuangyu Miao, Dongnan Li, Ye Zhang
Summary: In this study, a multiplex competitive annealing mediated isothermal amplification (mCAMP) assay on a microfluidic chip was developed for the visual detection of Cronobacter sakazakii in powdered infant formula (PIF) samples. The assay showed high specificity and a limit of detection of 2.2 x 10(3) CFU g(-1). The results are visible to the naked eye, making it suitable for rapid analysis in resource-limited settings.
Article
Agriculture, Dairy & Animal Science
Laipeng Xu, Xiangli Sun, Xianhua Wan, Keke Li, Fuchun Jian, Wenting Li, Ruirui Jiang, Ruili Han, Hong Li, Xiangtao Kang, Yanbin Wang
Summary: Clostridium butyricum is considered as an antibiotic substitute that can enhance antioxidant capacity, mucosal barrier function, and stabilize the cecal microbiota, resulting in improved growth performance in broilers.
Article
Multidisciplinary Sciences
Alexander T. Neu, Eric E. Allen, Kaustuv Roy
Summary: The term core microbiome refers to a set of microbial taxa or attributes associated with those taxa that are characteristic of a host or environment, with little consensus on how it should be quantified in practice. Various metrics have been used, but many are susceptible to biases, highlighting the need for standardized metrics for studying core microbiomes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Hao-En Jan, Chin-Shiang Tsai, Nan-Yao Lee, Pei-Fang Tsai, Li-Rong Wang, Po-Lin Chen, Wen-Chien Ko
Summary: This case report describes a rare infection of Francisella novicida in Taiwan, in a patient with end-stage renal disease and a history of other medical conditions. The patient recovered after antimicrobial therapy.
EMERGING MICROBES & INFECTIONS
(2022)