期刊
JOURNAL OF PEPTIDE SCIENCE
卷 18, 期 8, 页码 500-510出版社
WILEY-BLACKWELL
DOI: 10.1002/psc.2425
关键词
peptaibols; cytotoxicity; anthelmintic; Hypocreales; higher-energy collisional dissociation (HCD)
资金
- National Cancer Institute/National Institutes of Health, Bethesda, MD, USA [P01 CA125066]
- North Carolina Biotechnology Center [2011-CFG-8008]
- Kenan Institute for Engineering, Technology Science
As part of an ongoing investigation of filamentous fungi for anticancer leads, an active culture was identified from the Mycosynthetix library (MSX 70741, of the order Hypocreales, Ascomycota). The fungal extract exhibited cytotoxic activity against the H460 (human nonsmall cell lung carcinoma) cell line, and bioactivity-directed fractionation yielded peptaibols 112 and harzianums A (13) and B (14). Structure elucidation of 112 was facilitated by high-resolution MS/MS using higher-energy collisional dissociation and by high field NMR (950?MHz). The absolute configuration was determined by Marfey's analysis of the individual amino acids; the time required for such analysis was decreased via the development of a 10-min ultra performance liquid chromatography method. The isolated peptaibols (112), along with three other peptaibols isolated and elucidated from a different fungus (MSX 57715) of the same order (1517), were examined for activity in a suite of biological assays, including those for cytotoxic, antibacterial, and anthelmintic activities. Copyright (c) 2012 European Peptide Society and John Wiley & Sons, Ltd.
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