Article
Biochemistry & Molecular Biology
Li Li, Jiaxin Li, Xiaodan Yu, Ruipin Cao, Meiling Hong, Zuxian Xu, Jian Ren Lu, Yinglu Wang, Hu Zhu
Summary: This study demonstrated the potential of two newly designed antimicrobial peptides at subinhibitory concentrations as antiquorum-sensing agents against Pseudomonas aeruginosa. The peptides showed significant inhibition of QS-regulated virulence factors and biofilm formation, as well as downregulation of QS-related gene expression. The peptides induced structural alterations in the important QS-related protein LasR and bound to its hydrophobic groove.
BIOORGANIC CHEMISTRY
(2023)
Article
Agronomy
Somayeh Sadeqifard, Somayeh Mirmostafaee, Mohammad Reza Joharchi, Jaleh Zandavifard, Majid Azizi, Yoshiharu Fujii
Summary: Allelopathy is a physiological process where plants produce biologically active molecules that directly affect the growth and development of individuals of the same or other species. This study investigated the allelopathic activity of 123 medicinal and aromatic plants and explored their interaction effects. The results revealed different effects of the plants on the growth and development of lettuce, and demonstrated synergistic and antagonistic interactions among different plant species.
Article
Infectious Diseases
Tejas Suresh Khire, Wei Gao, Brian Bales, Kuangwen Hsieh, Greg Grossmann, Dong Jin M. Park, Christine O'Keefe, Arnyah Brown-Countess, Sara Peterson, Fan-En Chen, Ralf Lenigk, Alex Trick, Tza-Huei Wang, Christopher Puleo, Gabriele Bianco
Summary: Antimicrobial resistance is a global threat caused by the incorrect and overuse of antibiotics, resulting in the evolution of multi- and extreme drug-resistant bacterial strains. This study aims to provide phenotypic antimicrobial susceptibility testing information by combining short growth periods with PCR assays to predict minimum inhibitory concentration values of antibiotic treatment. The use of a single-vessel reaction system and microfluidic manifold allows for rapid point-of-care diagnostics.
Article
Food Science & Technology
Joana Gomes, Joana Barbosa, Paula Teixeira
Summary: This study aimed to evaluate the minimum inhibitory concentrations of three natural antimicrobial compounds against 37 microorganisms using agar dilution and drop diffusion methods. The results showed that lower pH values and the agar dilution method generally led to lower minimum inhibitory concentrations. However, the inhibition of different compounds varied under different conditions.
Article
Biochemistry & Molecular Biology
Jenna M. Greve, J. A. Cowan
Summary: Antibiotic resistance calls for innovative strategies and treatments. The tested antimicrobial peptides in this study exhibited broad-spectrum synergy and the ability to interact with various bacterial membranes. In most cases, the presence of the ATCUN motif enhanced the activity of the combined peptides. Additionally, the multifaceted peptide CP10A was found to have both cell membrane and intracellular activity, acting as a chameleon to enhance the activity of other peptides when needed. The results highlight the importance of diverse modes of action and the value of the metal-binding domain in enhancing the activity of antimicrobial peptides in combination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Jonatan A. N. Tillander, Karin Rilby, Karin Svensson Malchau, Susann Skovbjerg, Erika Lindberg, Ola Rolfson, Margarita Trobos
Summary: Prosthetic joint infections (PJIs) are a serious issue for patients and healthcare organizations, and current treatment methods have limited success rates. This study aims to evaluate the feasibility of an antibiotic regimen based on the minimum biofilm eradication concentration (MBEC) and explore the relationship between microbial properties, antimicrobial resistance, and infection outcomes.
Article
Microbiology
Troels Ronco, Line H. Kappel, Maria F. Aragao, Niccolo Biagi, Soren Svenningsen, Jorn B. Christensen, Anders Permin, Lasse Saaby, Kim Holmstrom, Janne K. Klitgaard, Artur J. Sabat, Viktoria Akkerboom, Monica Monaco, Marco Tinelli, Alexander W. Friedrich, Bimal Jana, Rikke H. Olsen
Summary: Multidrug-resistant pathogens are a serious global issue, and novel antimicrobials with new modes of action are urgently needed. In this study, a phenothiazine derivative (JBC 1847) was found to exhibit high antimicrobial activity against Staphylococcus aureus. The primary mechanism of action of JBC 1847 was the depolarization of the cell membrane, leading to the dissipation of the proton motive force and affecting essential bacterial processes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Yu Ri Kang, Doo Ryeon Chung, Jae-Hoon Ko, Kyungmin Huh, Sun Young Cho, Cheol-In Kang, Kyong Ran Peck
Summary: This study compared MSSA strains with high and low vancomycin MICs, and identified that mutations in the graR and walK genes may contribute to reduced vancomycin susceptibility in MSSA.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Environmental Sciences
Sirinthrar Wandee, Rathborey Chan, Wilai Chiemchaisri, Chart Chiemchaisri
Summary: The study found that in open pig farming systems, there is a high level of E. coli populations, antibiotic contamination in supplied water, and additional antibiotic usage, such as neomycin or colistin. Additionally, an increased occurrence of ARE was found in anaerobic digestion and waste stabilisation ponds, but they were not well correlated with their residual antibiotic concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Thea Brennan-Krohn, Alexandra Grote, Shade Rodriguez, James E. Kirby, Ashlee M. Earl
Summary: The combination of colistin and minocycline shows high synergy against resistant strains, as revealed by transcriptome sequencing findings.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Agriculture, Dairy & Animal Science
H. Taiyari, N. M. Faiz, J. Abu, Z. Zakaria
Summary: Although antibiotic treatment cannot eradicate avian mycoplasmosis, it can reduce the number of deaths. One major challenge of antibiotic treatments is the development of antimicrobial resistance among field isolates. Regular determination of minimum inhibitory concentrations of antibiotics is necessary to optimize treatment dosages.
JOURNAL OF APPLIED POULTRY RESEARCH
(2021)
Article
Infectious Diseases
Lucie Mala, Klara Lalouckova, Eva Skrivanova, Marketa Houdkova, Marie Strakova, Ladislav Kokoska
Summary: Bacterial skin diseases of livestock can be a serious global threat, and combination therapy with antimicrobial agents has been proven effective in overcoming bacterial resistance. This study found that zinc pyrithione in combination with gentamicin exhibited a strong synergistic effect against streptococcal strains, suggesting its potential use in the development of veterinary pharmaceutical preparations for the treatment of bacterial skin infections in livestock.
Article
Fisheries
Rute Irgang, Ruben Avendano-Herrera
Summary: This study analyzes the susceptibility patterns of Tenacibaculum dicentrarchi to tiamulin and suggests that tiamulin is a viable alternative to florfenicol for the treatment of tenacibaculosis in the Chilean salmon industry.
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Jielu Yan, Bob Zhang, Mingliang Zhou, Francois-Xavier Campbell-Valois, Shirley W. I. Siu
Summary: Antimicrobial peptides (AMPs) are potential candidates for replacing conventional antibiotics to combat drug resistance in pathogenic bacteria. To accelerate the evaluation process, we develop a deep learning method called MBC-Attention to predict the minimum inhibitory concentration of AMPs against Escherichia coli. The proposed model outperforms traditional machine learning methods.
Review
Chemistry, Multidisciplinary
Jessica T. Mhlongo, Ayman Y. Waddad, Fernando Albericio, Beatriz G. de la Torre
Summary: Antimicrobial peptides (AMPs) are promising candidates for combating multidrug resistance due to their heterogenous structures and broad-spectrum action. The combined use of AMPs with other antimicrobial agents provides a new arsenal of drugs with synergistic action, inhibiting the development of drug resistance. This review discusses various synergies of AMPs with antimicrobials and miscellaneous agents, providing guidance for improved clinical outcomes.
Article
Andrology
Lavanya Madugulla, Anandha Rao Ravula, Anand Kumar Kondapi, Suresh Yenugu
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2019)
Article
Endocrinology & Metabolism
Suresh Babu Munipalli, Suresh Yenugu
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2019)
Article
Reproductive Biology
Kumari Sangeeta, Suresh Yenugu
Article
Andrology
Suresh Babu Munipalli, Marri Reddy Mounika, Jamil Aisha, Suresh Yenugu
Article
Cell Biology
Kumari Sangeeta, Suresh Yenugu
CELL AND TISSUE RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Anandha R. Ravula, Suresh Yenugu
Summary: The study reveals that long-term exposure to a mixture of pyrethroids can lead to abnormalities in the transcriptome of factors involved in sperm production and development in rats.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Environmental Sciences
Anandha Rao Ravula, Suresh Yenugu
Summary: Studies on unintentional intake of pyrethroid pesticides similar to actual human exposure settings are rare. Exposure to low and high doses of pyrethroid mixture for 15 months resulted in abnormal lipid profile, liver function, and antioxidant enzyme activities, as well as damage to anatomical architecture in various tissues. This exposure also led to decreased sperm count, enzyme activities, impaired capacitation and acrosome reaction, and perturbations in gene expression related to male gamete production.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Toxicology
Anandha Rao Ravula, Suresh Yenugu
Summary: The use of pesticides has both benefits and risks to human health, with the main concern being their presence in water sources and the food chain. A review on pyrethroid-based insecticides shows their varying chemical and toxic nature, which can pose health hazards ranging from simple nausea to cancers. Studies focusing on the health hazards of pesticides at doses relevant to human exposure are lacking, suggesting a need for further research to provide concrete evidence on the risks of consuming pesticide contaminated food. Policy decisions to reduce pesticide residues in agricultural products and promote organic farming are recommended.
CRITICAL REVIEWS IN TOXICOLOGY
(2021)
Review
Obstetrics & Gynecology
Patra Priyadarshini Priyanka, Suresh Yenugu
Summary: CCDC proteins with coiled-coil domains play crucial roles in various physiological and pathological processes, influencing important biological functions, cancer pathogenesis, and lifetime disease risk. Extensively studied, there is ample opportunity for further exploration of their involvement in different functions in the future.
REPRODUCTIVE SCIENCES
(2021)
Article
Cell Biology
Kumari Sangeeta, Suresh Yenugu
Summary: This study found that ablation of SPAG11A by active immunization renders the epididymis susceptible to oncogenesis, and this protein may be one of the factors that contributes to the rarity of epididymal cancer.
CELL AND TISSUE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Anandha R. Ravula, Suresh Yenugu
Summary: Residual amounts of pyrethroids were found in rice and vegetables in the Indian market, posing a daily exposure risk to consumers. A study on male Wistar rats exposed to pyrethroid mixture equivalent to human consumption levels revealed increased oxidative stress, disruptions in antioxidant enzyme activities, and anatomical damage to organs and male reproductive tissues. This exposure was also found to affect liver function and lipid profile. The findings indicate that exposure to pyrethroids present in food can lead to systemic and reproductive toxicity, emphasizing the need for further research and policy measures to reduce pesticide levels in food products.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Andrology
Patra Priyadarshini Priyanka, Anandha Rao Ravula, Suresh Yenugu
Summary: The misuse of pesticides has led to the contamination of food products, with residual amounts of pyrethroids being detected in agricultural products sold in the Indian market. A study on male rats showed that long-term exposure to a mixture of pyrethroids resulted in reduced fertility and sperm count, altered hormonal concentrations related to male reproductive function, and testicular genotoxicity. These toxic effects may also occur in humans who consume agricultural products containing residual amounts of pyrethroids throughout their lifetime.
Article
Obstetrics & Gynecology
Suresh Babu Munipalli, Suresh Yenugu
Summary: Uroplakins (UPKs) play important roles in forming barriers and maintaining tissue integrity in the bladder and other urinary tract tissues. This study investigated the impact of Upk1a gene knockout in mice, revealing reduced fecundity and sperm count, as well as impaired bacterial clearance capacity. Transcriptome analysis of the testes identified differentially expressed genes associated with various biological processes. These findings suggest that Upk1a has multiple functions in the male reproductive tract and other tissues.
REPRODUCTIVE SCIENCES
(2023)
Article
Obstetrics & Gynecology
Suresh Babu Munipalli, Suresh Yenugu
Summary: In this study, yeast two-hybrid screening was used to identify the interaction partners of UPK1A, and Regucalcin (RGN) and proteasome subunit beta 1 (PSMB1) were discovered as potential interaction partners. The co-localization and interaction between UPK1A and RGN or PSMB1 were confirmed through high-resolution microscopy and Pearson's coefficient. The interaction between UPK1A and RGN or PSMB1 may be necessary for spermatogenesis, considering the subfertility caused by UPK1A knockout and the documented roles of RGN and PSMB1 in spermatogenesis.
REPRODUCTIVE SCIENCES
(2023)
Article
Microbiology
Roja Rani Pallavali, Vijaya Lakshmi Degati, Venkata Ramireddy Narala, Kiran Kumar Velpula, Suresh Yenugu, Vijaya Raghava Prasad Durbaka
Summary: The study demonstrated that under dynamic growth conditions, bacteriophages can effectively reduce biofilm formation by Staphylococcus aureus, Pseudomona aeruginosa, Klebsiella pneumoniae, and Escherichia coli. These results support the concept of developing bacteriophages as alternatives to antibiotics for treating bacterial infections.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2021)