期刊
JOURNAL OF PEDIATRIC SURGERY
卷 44, 期 4, 页码 759-766出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.jpedsurg.2008.06.006
关键词
Hirschsprung's disease; Hirschsprung's-associated enterocolitis; Aganglionic megacolon; Microsurgery; Pullthrough; Ednrb-B-null mice
资金
- Lippey Family Endowment
- Walter and Shirley Wang Endowed Chair in Pediatric Surgery at Cedars-Sinai Medical Center
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [K08DK090281] Funding Source: NIH RePORTER
Background/Purpose: The study aimed to develop a mouse model of post-pullthrough Hirschsprung's disease that will allow investigation of mechanisms that Cause postoperative complications. Methods: We developed a novel microsurgical pullthrough operation on Balb/C mice and evaluated its effect on growth rate and stooling pattern. Histologic assessment of the pullthrough colon was performed. The pullthrough operation was then performed on Ednrb-/- mice that have aganglionic megacolon and Ednrb+/+ littermate controls, and the outcomes compared. Results: The Balb/C pullthrough group had 97% survival at I week and 70% survival at 2 weeks. Body weight of the pullthrough animals declined 15% in the first week after surgery and Subsequently normalized. The stooling pattern showed consistently softer stools in the pullthrough group, but no difference in frequency compared to controls. Histopathologic analyses 4 weeks postoperatively showed well-healed coloanal anastomoses. Two-week survival after pullthrough surgery in Ednrb-/- and Ednrb+/+ mice was 50.0%, and 69.2%, respectively (P = NS). Increased mortality in the Ednrb-/- mice was related to the technical challenge of performing microsurgery on smaller-sized mice with poor baseline health status. Conclusions: Our microsurgical pullthrough operation in mice is feasible and allows systematic investigations into potential mechanisms mediating post-pullthrough complications and poor long-term results in mouse models of Hirschsprung's disease. (C) 2009 Elsevier Inc. All rights reserved.
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