Article
Oncology
Benjamin M. Vierra, Lily V. Saadat, Jason L. Hornick, Jyothi P. Jagannathan, Marco L. Ferrone, Andrew J. Wagner, Jiping Wang, Elizabeth H. Baldini, Chandrajit Raut, Mark Fairweather
Summary: This study investigated the rate and distribution of spine metastases in patients with myxoid liposarcoma (LPS) and found that spine metastases were common and associated with worse overall survival in these patients. Routine surveillance with spine MRI may not provide benefits for patients with localized disease but can be considered for those with known metastatic disease.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Chemistry, Analytical
Florent Penen, Rene Raave, Annemarie Kip, Sandra Heskamp, Per Malmberg
Summary: ToF-SIMS imaging is a quick and sensitive technique that can visualize HER2 specific radiotracers in ovarian and breast cancer xenograft tumor sections to reflect the heterogeneous distribution of trastuzumab.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Teresa Murta, Rory T. Steven, Chelsea J. Nikula, Spencer A. Thomas, Lucas B. Zeiger, Alex Dexter, Efstathios A. Elia, Bin Yan, Andrew D. Campbell, Richard J. A. Goodwin, Zoltan Takats, Owen J. Sansom, Josephine Bunch
Summary: Mass spectrometry imaging generates complex spectral and spatial data, which are often analyzed using unsupervised machine learning techniques. This study focuses on the impact of peak selection choices on the interpretation of analyses, highlighting the importance of selecting peaks based on specific criteria for more valuable and easier-to-interpret results.
ANALYTICAL CHEMISTRY
(2021)
Article
Biology
Konstantin Drexler, Katharina M. Schmidt, Katrin Jordan, Marianne Federlin, Vladimir M. Milenkovic, Gerhard Liebisch, Anna Artati, Christian Schmidl, Gregor Madej, Janina Tokarz, Alexander Cecil, Wolfgang Jagla, Silke Haerteis, Thiha Aung, Christine Wagner, Maria Kolodziejczyk, Stefanie Heinke, Evan H. Stanton, Barbara Schwertner, Dania Riegel, Christian H. Wetzel, Wolfgang Buchalla, Martin Proescholdt, Christoph A. Klein, Mark Berneburg, Hans J. Schlitt, Thomas Brabletz, Christine Ziegler, Eric K. Parkinson, Andreas Gaumann, Edward K. Geissler, Jerzy Adamski, Sebastian Haferkamp, Maria E. Mycielska
Summary: Citrate is essential for cancer cell metabolism, supplied by cancer-associated stroma (CAS) and controlled by cancer cells for progression and invasion. Depriving cancer cells of citrate significantly reduces growth and metastasis, indicating the importance of citrate uptake in cancer progression.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemistry & Molecular Biology
Lucy E. Flint, Gregory Hamm, Joseph D. Ready, Stephanie Ling, Catherine J. Duckett, Neil A. Cross, Laura M. Cole, David P. Smith, Richard J. A. Goodwin, Malcolm R. Clench
Summary: This study employed mass spectrometry imaging (MSI) modalities to characterize two aggregated cellular models of osteosarcoma, revealing fatty acid and phospholipid markers relevant to human tissue samples. The results suggested that the MG63 aggregoids were aggressive tumor models with metastatic-like potential, while the SAOS-2 aggregoids exhibited characteristics of cellular differentiation and bone development. Both OS aggregoid models shared similarities of metabolic behavior with different regions of OS human tissues.
Article
Chemistry, Analytical
Zdravko Siketic, Iva Bogdanovic Radovic, Marko Barac, Marko Brajkovic, Marijana Popovic Hadzija
Summary: MeV SIMS is a type of secondary ion mass spectrometry (SIMS) technique that uses MeV energy ions to desorb molecules from a sample surface. This study presents a new system for molecular imaging of organic materials using a continuous analytical beam and a start trigger based on the detection of secondary electrons. The system allows for the imaging of samples with a lateral resolution of about 20 μm and the analysis of samples of any thickness. The imaging capabilities of MeV SIMS are demonstrated on various samples to show its potential applications in biology and forensic science.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Alex M. Jaeger, Lauren E. Stopfer, Ryuhjin Ahn, Emma A. Sanders, Demi A. Sandel, William A. Freed-Pastor, William M. Rideout, Santiago Naranjo, Tim Fessenden, Kim B. Nguyen, Peter S. Winter, Ryan E. Kohn, Peter M. K. Westcott, Jason M. Schenkel, Sean-Luc Shanahan, Alex K. Shalek, Stefani Spranger, Forest M. White, Tyler Jacks
Summary: This study engineered an inducible affinity tag into the mouse MHC-I gene to precisely isolate MHC-I peptides from autochthonous pancreatic ductal adenocarcinoma and lung adenocarcinoma in vivo. It revealed that peptide presentation in lung adenocarcinoma is not predictable by mRNA expression or translation efficiency and may be driven by post-translational mechanisms. Vaccination with peptides presented by lung adenocarcinoma induced CD8(+) T cell responses.
Article
Medicine, General & Internal
Thuy-Linh Tran, Irene Tsai, Hyung Won Choi
Summary: Metastasis to the breast from non-mammary malignancies is rare and often indicative of advanced disease. Accurate diagnosis and timely treatment of breast metastasis are crucial for prognosis and treatment planning. Interestingly, unlike primary breast malignancies, non-mammary metastatic breast lesions typically exhibit benign characteristics on imaging. Knowledge of the patient's clinical history and comparison with previous breast imaging studies are essential for accurate assessment and appropriate recommendations. Imaging-guided biopsy is frequently necessary for definitive tissue diagnosis.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Oncology
Nuria Vaquero-Siguero, Nikolai Schleussner, Julia Volk, Manuel Mastel, Jasmin Meier, Rene Jackstadt
Summary: Colorectal cancer is one of the deadliest cancers globally, with metastasis being the main cause of patient mortality. The complex tumor ecosystem undergoes compositional changes at virtually every stage of CRC progression. However, clonal dynamics and associated niche-dependencies at these stages are currently unknown.
Article
Biochemistry & Molecular Biology
P. Bernhard, T. Feilen, M. Rogg, K. Froehlich, M. Cosenza-Contreras, F. Hause, C. Schell, O. Schilling
Summary: Clonal isolation is a crucial step in genome editing and cell engineering, impacting the cellular proteome in a cell line specific manner. The effects of clonal isolation on different biological processes vary and depend on the isolation method. Understanding the impact of clonal isolation is important when interpreting experimental data in these fields.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Chenyu Yang, Ran Wu, Haiqiang Liu, Liang Qin, Lulu Chen, Hualei Xu, Hao Hu, Jinrong Li, Hua Guo, Yiyang Shi, Dongxu Jiang, Qichen Hao, Jinchao Feng, Yijun Zhou, Xiangyi Liu, Gaopeng Li, Xiaodong Wang
Summary: In this study, polyacrylamide gel (PAAG) was successfully used as a new embedding medium for enhancing tissue imaging of metabolites via matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The results showed that PAAG embedding has advantages over commonly-used embedding media, including better morphology maintenance, absence of interference below m/z 2000, and more efficient ionization of metabolites. PAAG embedding has the potential to become a standard practice for metabolite MALDI tissue imaging, expanding the application scope of MALDI-MSI.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yiyang Shi, Hao Hu, Qichen Hao, Ran Wu, Lei Wang, Liang Qin, Wei Gu, Haiqiang Liu, Dongxu Jiang, Liya Hong, Yijun Zhou, Xiangyi Liu, Jinchao Feng, Kun Xue, Xiaodong Wang
Summary: Michler's ethylketone (MEK) has been successfully optimized as a novel matrix for the enhancement of lipid detection and imaging in tissues by MALDI-MSI. Its properties as a powerful MALDI matrix include strong UV absorption, mu m-sized crystals, uniform matrix-coating, super high vacuum chemical stability, low matrix-related ion interference, super soft ionization, and high lipid ionization efficiency.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Weiwei Tang, Jun-Jie Shi, Wei Liu, Xu Lu, Bin Li
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was applied to discover a glycosyltransferase (GT) named PgGT1 from the root of Platycodon grandiflorum. PgGT1 was found to catalyze the synthesis of platycoside E (PE) by sequentially attaching two beta-1,6-linked glucosyl residues to the glucosyl residue at the C3 position of platycodin D (PD). The study also revealed the crucial roles of residues S273, E274, and H350 in stabilizing the sugar donor and positioning the glucose for optimal glycosylation. These findings provide insights into the biosynthetic pathway of PE and have potential implications in industrial biotransformation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Simone Ciccolella, Camir Ricketts, Mauricio Soto Gomez, Murray Patterson, Dana Silverbush, Paola Bonizzoni, Iman Hajirasouliha, Gianluca Della Vedova
Summary: Recent studies have identified limitations in current computational methods for inferring tumor phylogenies. The new SASC approach, based on simulated annealing and using the Dollo-k model, demonstrates high accuracy in inferring cancer progressions.
Article
Pathology
Xudong Wang, Xiaohui Zhang, Wenling Pan, Yuedong Han, Yanwei Li, Haibin Sun, Pancras C. W. Hogendoorn, Hong Cheng
Summary: The metastasis of lung squamous cell carcinoma to an enchondroma in the femur of a patient with Ollier disease is an extremely rare occurrence. Thorough clinical and radiological information is crucial for accurate diagnosis, while immunohistochemistry can be used to confirm the difference in lineage.
Article
Pathology
Paul G. Kemps, Konnie M. Hebeda, Steven T. Pals, Robert M. Verdijk, King H. Lam, Annette H. Bruggink, Heleen S. de Lil, Bart Ruiterkamp, Koen de Heer, Jan A. M. van Laar, Peter J. M. Valk, Pim Mutsaers, Mark-David Levin, Pancras C. W. Hogendoorn, Astrid G. S. van Halteren
Summary: Research has found associations between histiocytic disorders and additional hematological malignancies with the same genetic alterations, indicating a common hematopoietic cell-of-origin in these patients. This highlights the importance of adequate staging, molecular analysis, and long-term follow-up of all histiocytosis patients.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2021)
Article
Pathology
Rohola Hosseini, Gerda E. M. Lamers, Erik Bos, Pancras C. W. Hogendoorn, Abraham J. Koster, Annemarie H. Meijer, Herman P. Spaink, Marcel J. M. Schaaf
Summary: The study used zebrafish larvae infected with Mycobacterium marinum as an animal model for tuberculosis. The results showed that Myd88 deficiency led to more compact granuloma structures and a reduced number of leukocytes. Additionally, the majority of bacteria in the Myd88 mutant were located extracellularly, while most bacteria in the wild type were intracellular.
Article
Cell Biology
Suk Wai Lam, Inge H. Briaire-de Bruijn, Tom van Wezel, Arjen H. G. Cleven, Pancras C. W. Hogendoorn, Anne-Marie Cleton-Jansen, Judith V. M. G. Bovee
Summary: This study aimed to investigate the frequency of NTRK fusions in bone tumors, and the results showed that the likelihood of finding an NTRK fusion in bone tumors is extremely low.
Article
Hematology
Paul G. Kemps, Jennifer Picarsic, Benjamin H. Durham, Zofia Helias-Rodzewicz, Laura Hiemcke-Jiwa, Cor van den Bos, Marianne D. van de Wetering, Carel J. M. van Noesel, Jan A. M. van Laar, Robert M. Verdijk, Uta E. Flucke, Pancras C. W. Hogendoorn, F. J. Sherida H. Woei-A-Jin, Raf Sciot, Andreas Beilken, Friedrich Feuerhake, Martin Ebinger, Robert Mohle, Falko Fend, Antje Bornemann, Verena Wiegering, Karen Ernestus, Tina Mery, Olga Gryniewicz-Kwiatkowska, Bozenna Dembowska-Baginska, Dmitry A. Evseev, Vsevolod Potapenko, Vadim V. Baykov, Stefania Gaspari, Sabrina Rossi, Marco Gessi, Gianpiero Tamburrini, Sebastien Heritier, Jean Donadieu, Jacinthe Bonneau-Lagacherie, Claire Lamaison, Laure Farnault, Sylvie Fraitag, Marie-Laure Jullie, Julien Haroche, Matthew Collin, Jackie Allotey, Majid Madni, Kerry Turner, Susan Picton, Pasquale M. Barbaro, Alysa Poulin, Ingrid S. Tam, Dina El Demellawy, Brianna Empringham, James A. Whitlock, Aditya Raghunathan, Amy A. Swanson, Mariko Suchi, Jon M. Brandt, Nabeel R. Yaseen, Joanna L. Weinstein, Irem Eldem, Bryan A. Sisk, Vaishnavi Sridhar, Mandy Atkinson, Lucas R. Massoth, Jason L. Hornick, Sanda Alexandrescu, Kee Kiat Yeo, Kseniya Petrova-Drus, Stephen Z. Peeke, Laura S. Munoz-Arcos, Daniel G. Leino, David D. Grier, Robert Lorsbach, Somak Roy, Ashish R. Kumar, Shipra Garg, Nishant Tiwari, Kristian T. Schafernak, Michael M. Henry, Astrid G. S. van Halteren, Oussama Abla, Eli L. Diamond, Jean-Francois Emile
Summary: ALK-positive histiocytosis is a rare and poorly characterized subtype of histiocytic neoplasm. This study provides the largest analysis of ALK-positive histiocytosis to date, describing the clinicopathologic and molecular characteristics of 39 cases. The study also highlights the robust responses observed in patients treated with ALK inhibition, particularly those with neurologic involvement.
Article
Biochemistry & Molecular Biology
Ramzi Khalil, Margien G. S. Boels, Palga Group, Bernard M. van den Berg, Jan A. Bruijn, Ton J. Rabelink, Pancras C. W. Hogendoorn, Hans J. Baelde
Summary: This study investigates the effect of heterozygous germline mutations in the EXT1 and EXT2 genes on the glomerular barrier function and the endothelial glycocalyx in humans. The results show that these mutations do not lead to (micro)albuminuria, specific renal phenotype or changes to the endothelial glycocalyx in humans. These findings contribute to the understanding of the role of EXT1 and EXT2 genes in pathophysiology.
MOLECULAR GENETICS AND GENOMICS
(2022)
Article
Pathology
Li Yang, Hongjuan Zhang, Xiaohui Zhang, Yongqiang Tang, Zhigang Wu, Yingmei Wang, Hai Huang, Xin Fu, Jiayan Liu, Pancras C. W. Hogendoorn, Hong Cheng
Summary: Denosumab-treated GCTB exhibits histological changes such as depletion of giant cells and intralesional bone deposition, which may lead to diagnostic difficulties. The post-therapy tumors have a similar mutation profile to classic GCTB but significantly differ morphologically. Awareness of the features of DT-GCTB and conducting immunohistochemical and molecular studies for histone H3 mutations are important in its assessment.
ANNALS OF DIAGNOSTIC PATHOLOGY
(2022)
Article
Oncology
Fanny Boyaval, Hans Dalebout, Rene Van Zeijl, Wenjun Wang, Arantza Farina-Sarasqueta, Guinevere S. M. Lageveen-Kammeijer, Jurjen J. Boonstra, Liam A. McDonnell, Manfred Wuhrer, Hans Morreau, Bram Heijs
Summary: This study investigates the changes in sugar molecules during the progression of colorectal cancer (CRC) and finds that these sugar signatures can clearly differentiate different stages of CRC progression, especially between pre-cancerous and cancerous tissue. The study also observes the spreading of glycosylation signatures into the tumor microenvironment.
Article
Oncology
David J. Barnes, Peter Dutton, Oyvind Bruland, Hans Gelderblom, Ade Faleti, Claudia Buhnemann, Annemiek van Maldegem, Hannah Johnson, Lisa Poulton, Sharon Love, Gesa Tiemeier, Els van Beelen, Karin Herbschleb, Caroline Haddon, Lucinda Billingham, Kevin Bradley, Stefano Ferrari, Emanuela Palmerini, Piero Picci, Uta Dirksen, Sandra J. Strauss, Pancras C. W. Hogendoorn, Emmeline Buddingh, Jean-Yves Blay, Anne Marie Cleton-Jansen, Andrew Bassim Hassan
Summary: This study reports a Bayesian designed phase II trial that investigates the efficacy of liposomal MTP-PE alone or in combination with ifosfamide in patients with metastatic and/or recurrent osteosarcoma. Despite poor recruitment and no objective responses, the study demonstrates the feasibility of the Bayesian design and the collection of tissue and serum samples for further analysis. Further investigation of liposomal MTP-PE in metastatic osteosarcoma is warranted.
Article
Oncology
Sanne Venneker, Robin van Eenige, Alwine B. Kruisselbrink, Ieva Palubeckaite, Alice E. Taliento, Inge H. Briaire-de Bruijn, Pancras C. W. Hogendoorn, Michiel A. J. van de Sande, Hans Gelderblom, Hailiang Mei, Judith V. M. G. Bovee, Karoly Szuhai
Summary: Giant cell tumor of bone (GCTB) is a bone neoplasm characterized by mutated neoplastic stromal cells. This study investigated the potential of histone deacetylase (HDAC) inhibitors as a therapeutic strategy to eliminate these neoplastic cells. The findings suggest that HDAC inhibition may improve the quality of life for GCTB patients who require life-long treatment.
Review
Oncology
Djoko Santoso, Mochammad Thaha, Maulana A. Empitu, Ika Nindya Kadariswantiningsih, Satriyo Dwi Suryantoro, Mutiara Rizki Haryati, Decsa Medika Hertanto, Dana Pramudya, Siprianus Ugroseno Yudho Bintoro, Nasronudin Nasronudin, Mochamad Yusuf Alsagaff, Hendri Susilo, Citrawati Dyah Kencono Wungu, Nicolaas C. Budhiparama, Pancras C. W. Hogendoorn
Summary: Osteitis fibrosa cystica (OFC) and Brown Tumours are rare bone lesions associated with chronic kidney disease. The mechanism of these conditions is not well understood, but recent literature suggests that factors such as overactivation of the renin-angiotensin-aldosterone system and chronic inflammation may contribute to their occurrence through osteoclast activation. In addition, hotspot KRAS mutations have been identified in these lesions, placing them in the spectrum of RAS-MAPK-driven neoplasms. Risk factors for OFC and Brown Tumours include age, gender, comorbidities, and certain medications. Diagnosis involves clinical symptoms of chronic bone pain and laboratory findings of hyperparathyroidism, as well as radiological imaging and histological examination.
Article
Physiology
Ramzi Khalil, Josephine D. D. Bonnemaijer, Reinhold Kreutz, Herman P. Spaink, Pancras C. W. Hogendoorn, Hans J. Baelde
Summary: Transmembrane protein 14A (TMEM14A) is an important protein involved in maintaining the integrity of the glomerular filtration barrier. It suppresses Bax-mediated apoptosis and its expression is increased in proteinuric renal diseases.
PHYSIOLOGICAL REPORTS
(2023)
Review
Immunology
Marloes M. A. R. van Dorst, Jeremia J. Pyuza, Gyaviira Nkurunungi, Vesla I. Kullaya, Hermelijn H. Smits, Pancras C. W. Hogendoorn, Linda J. Wammes, Bart Everts, Alison M. Elliott, Simon P. Jochems, Maria Yazdanbakhsh
Summary: There is considerable variation in efficacy of vaccines across populations and geographical regions, which is influenced by multiple environmental factors. Current interventions targeting these factors have had variable success, highlighting the need for a better understanding of the immunological mechanisms involved in vaccine responses.
NATURE REVIEWS IMMUNOLOGY
(2023)
Meeting Abstract
Oncology
Paul Kemps, Jennifer Picarsic, Benjamin H. Durham, Zofia Helias-Rodzewicz, Laura Hiemcke-Jiwa, Cor van den Bos, Marianne D. van Dewetering, Carel J. M. van Noesel, Pancras C. W. Hogendoorn, F. J. Sherida H. Woei-A-Jin, Raf Sciot, Andreas Beilken, Friedrich Feuerhake, Martin Ebinger, Robert Mohle, Falko Fend, Antje Bornemann, Verena Wiegering, Karen Ernestus, Tina Mery, Olga Gryniewicz-Kwiatkowska, Bozenna Dembowska-Baginska, Dmitry A. Evseev, Vsevolod Potapenko, Vadim V. Baykov, Stefania Gaspari, Sabrina Rossi, Marco Gessi, Gianpiero Tamburrini, Sebastien Heritier, Jacinthe Bonneau-Lagacherie, Claire Lamaison, Laure Farnault, Sylvie Fraitag, Jean Donadieu, Julien Haroche, Matthew Collin, Jackie Allotey, Majid Madni, Kerry Turner, Susan Picton, Pasquale M. Barbaro, Dina El Demellawy, Brianna Empringham, James A. Whitlock, Aditya Raghunathan, Amy A. Swanson, Mariko Suchi, Jon M. Brandt, Nabeel R. Yaseen, Joanna L. Weinstein, Irem Eldem, Bryan A. Sisk, Vaishnavi Sridha, Mandy Atkinson, Lucas R. Massoth, Jason L. Hornick, Sanda Alexandrescu, Kee Kiat Yeo, Kseniya Petrova-Drus, Stephen Z. Peeke, Laura S. Munoz-Arcos, Daniel G. Leino, David D. Grier, Robert Lorsbach, Somak Roy, Ashish R. Kumar, Shipra Garg, Nishant Tiwari, Kristian T. Schafernak, Michael M. Henry, Astrid G. S. van Halteren, Oussama Abla, Eli L. Diamond, Jean-Francois Emile
PEDIATRIC BLOOD & CANCER
(2022)
Review
Medicine, General & Internal
Alex Mremi, Jeremia J. Pyuza, Bilal Ahmad, Alice A. Andongolile, Adnan Sadiq, James J. Yahaya, Karoly Szuhai, Pancras C. W. Hogendoorn
Summary: Primary non-Hodgkin lymphoma of bone is a rare neoplasm composed of malignant lymphoid cells, and delay in diagnosis and treatment can significantly impact patient prognosis. A case study reported a 13-year-old male with this condition, who unfortunately passed away shortly after diagnosis due to the COVID-19 outbreak, highlighting the importance of timely follow-up and treatment for patients with this disease.
OXFORD MEDICAL CASE REPORTS
(2021)