Article
Cell & Tissue Engineering
Jingqiong Xun, Chan Li, Meilu Liu, Yueming Mei, Qiongfei Zhou, Bo Wu, Fen Xie, Yuling Liu, Ruchun Dai
Summary: The study found that bone microdamage increased in aged osteoporotic rats after fatigue loading, and the osteogenic differentiation ability of bone mesenchymal stem cells decreased. However, treatment with serum exosomes from young rats highly expressing miRNA-19b-3p was able to improve this decreased osteogenic differentiation ability.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Svetlana Rodimova, Artem Mozherov, Vadim Elagin, Maria Karabut, Ilya Shchechkin, Dmitry Kozlov, Dmitry Krylov, Alena Gavrina, Vladislav Kaplin, Evgenii Epifanov, Nikita Minaev, Ksenia Bardakova, Anna Solovieva, Peter Timashev, Elena Zagaynova, Daria Kuznetsova
Summary: Homogeneous and heterogeneous 3D scaffolds were fabricated and their osteoinductive properties were evaluated through in vitro and in vivo experiments. The results showed that the heterogeneous scaffolds exhibited improved osteoinductive properties and enhanced bone tissue recovery. Furthermore, the use of label-free bioimaging methods was considered effective for assessing the differentiation efficiency of MSCs on scaffolds.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Medicine, Research & Experimental
Maria Rosa Iaquinta, Carmen Lanzillotti, Chiara Mazziotta, Ilaria Bononi, Francesca Frontini, Elisa Mazzoni, Lucia Oton-Gonzalez, John Charles Rotondo, Elena Torreggiani, Mauro Tognon, Fernanda Martini
Summary: Mesenchymal stem cells (MSCs) play a crucial role in tissue engineering and regenerative medicine for bone tissue and cartilage. The regulation of miRNAs is important for MSCs differentiation, and dysregulation of miRNAs may be associated with osteoporosis, bone fractures, and bone cancer. Bone-associated circulating miRNAs could be useful biomarkers for diagnosis, follow-up, and treatment of cancer and metastases.
Article
Engineering, Biomedical
Zhipo Du, Xinxing Feng, Guangxiu Cao, Zhending She, Rongwei Tan, Katerina E. Aifantis, Ruihong Zhang, Xiaoming Li
Summary: The study found that multi-walled carbon nanotubes (MCNTs) were more effective in activating specific signaling pathways than nano-hydroxyapatite (nHA) and could better promote osteogenic differentiation of human adipose-derived mesenchymal stem cells (HASCs). In in vivo experiments, MCNTs were also found to induce ectopic bone formation better than nHA.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Mariia S. Saveleva, Alexey N. Ivanov, Julia A. Chibrikova, Anatolii A. Abalymov, Maria A. Surmeneva, Roman A. Surmenev, Bogdan Parakhonskiy, Maria Lomova, Andre G. Skirtach, Igor A. Norkin
Summary: The study investigates the osteogenic and therapeutic potential of bioactive vaterite as a functional component of a fibrous polymeric scaffold for bone regeneration. Results show that vaterite accelerates new bone tissue formation and facilitates bone defect repair.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Lunhao Li, Yiyu Peng, Qingyue Yuan, Jing Sun, Ai Zhuang, Xiaoping Bi
Summary: The study demonstrated that LL37 promotes osteogenic differentiation in vitro and exhibits antibacterial properties. The combined scaffold of PSeD/hADSCs/LL37 showed superior osteogenic properties in the rat calvarial defect model, indicating high potential in clinical bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Yibo Zhang, Peng Wang, Haijun Mao, Yangheng Zhang, Liming Zheng, Pengjun Yu, Zhirui Guo, Lan Li, Qing Jiang
Summary: This study comprehensively evaluated the effects of PEGylated gold nanoparticles of different sizes on osteogenic differentiation, demonstrating excellent biocompatibility and size-dependent promotion of osteogenic effects on various cell types. The Wnt/beta-catenin signaling pathway was found to play a crucial role in GNPs-induced osteogenic differentiation. Further in vivo testing showed that 45-nm GNPs conjugated with PEG-hydrogel significantly stimulated bone regeneration in rabbits with femur defects, indicating promising applications for clinical translation.
MATERIALS & DESIGN
(2021)
Article
Engineering, Biomedical
Jiangfeng Li, Chunyi Wang, Guoxing Gao, Xing Yin, Ximing Pu, Bing Shi, Yang Liu, Zhongbing Huang, Juan Wang, Jingtao Li, Guangfu Yin
Summary: Achieving a balance between biodegradability and osteogenic capacity in biosynthetic bone grafts remains a challenge. In this study, we addressed this challenge by incorporating mesoporous bioactive glass (MBG) into poly(caprolactone-co-glycolide) (PGA-PCL) at gradient ratios. The MBG/PGA-PCL (PGC/M) scaffolds exhibited a highly porous structure with incorporated MBGs uniformly dispersed. The incorporation of MBG significantly improved the scaffold's surface properties, apatite-formation ability, pH stability, and promoted the attachment, spreading, viability, and proliferation of bone marrow stromal cells (BMSCs) on the scaffolds. Moreover, the scaffolds demonstrated enhanced ALP activity and osteogenesis-related gene expression in a dose-dependent manner. When implanted into rat cranial bone defects, the scaffolds with higher MBG incorporation ratios induced significantly better bone repair. These findings suggest that the PGC/M scaffolds are promising biomaterials for bone repair with tunable properties.
BIOACTIVE MATERIALS
(2022)
Article
Multidisciplinary Sciences
Lan Ma, Yijun Yu, Hanxiao Liu, Weibin Sun, Zitong Lin, Chao Liu, Leiying Miao
Summary: The study prepared berberine/polycaprolactone/collagen (BBR/PCL/COL) scaffolds with different concentrations of berberine through electrospinning, and systematically studied the influence of dosage on scaffold morphology, cell behavior, and in vivo bone defect repair. The results indicated that the scaffolds had stable release ability, good biocompatibility, and promoted osteogenic differentiation of dental pulp stem cells. The scaffold showed potential for tissue engineering applications in bone regeneration therapy.
SCIENTIFIC REPORTS
(2021)
Article
Nanoscience & Nanotechnology
Ruyin Lyu, Yuping Chen, Yajun Shuai, Jie Wang, Leihao Lu, Qichao Cheng, Jiangfeng Cai, Chuanbin Mao, Mingying Yang
Summary: We demonstrate a universal strategy for discovering peptides that can bind to and functionalize biomaterials using a Bombyx mori silk fibroin membrane as a model. By fusing two peptides, one screened for binding to the biomaterial and another derived from a growth factor, a new chimeric peptide is created to efficiently bind to the silk fibroin membrane. The chimeric peptide enables the membrane to induce osteogenic differentiation of human mesenchymal stem cells and stimulate bone regeneration in a rat model without causing inflammation.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Veterinary Sciences
Hlaing Phyo, Amira Aburza, Katie Mellanby, Cristina L. L. Esteves
Summary: Researchers found that the endometrium of dogs can be used as an alternative source of mesenchymal stem cells (MSCs), which have similar characteristics to MSCs obtained from adipose tissue for therapeutic applications.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hyung-Mun Yun, Mi Hyeon Cho, Hoibin Jeong, Soo Hyun Kim, Yun Hee Jeong, Kyung-Ran Park
Summary: This study investigated the effects of a flavonoid called trifolirhizin (trifol) derived from Sophora flavescens on osteogenic processes during osteoblast differentiation. It was found that trifol promoted osteoblast differentiation markers and stimulated major osteogenic signaling proteins. Trifol also increased osteoblast-mediated bone-forming phenotypes. These findings suggest that trifol may be an effective phytomedicine for bone diseases such as osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Jen-Chieh Lai, Hsin-Pei Li, Gary Ro-Lin Chang, Ying-Wei Lan, Yu-Hsuan Chen, Yan-Shen Tseng, Min-Yu Tu, Chien-Fu Chen, Hsiao-Ling Chen, Chuan-Mu Chen
Summary: This study found that kefir peptides (KPs) can promote fracture healing by promoting osteogenic differentiation and may be used as a nutritional supplement to accelerate healing.
Article
Chemistry, Multidisciplinary
Na Ren, Na Liang, Mengwei Dong, Zhichao Feng, Ling Meng, Chunhui Sun, Aizhu Wang, Xin Yu, Wenhan Wang, Juan Xie, Chao Liu, Hong Liu
Summary: In this study, researchers directed the differentiation of mesenchymal stem cells (MSCs) towards osteoblast lineage by using nanoscale zeolitic imidazolate framework-8 (ZIF-8) nanoparticles. They encapsulated the ZIF-8 nanoparticles with stem cell membranes (SCMs) to improve biocompatibility and targeted ability towards MSCs. The internalized SCM/ZIF-8 nanoparticles demonstrated excellent biocompatibility and increased osteogenic potentials of MSCs. Furthermore, the activated cyclic adenosine 3,5-monophosphate (cAMP)-PKA-CREB signaling pathway was found to dominate in accelerating osteogenic differentiation. In vivo experiments showed that SCM/ZIF-8 nanoparticles greatly promoted the formation of new bone tissue.
Article
Biochemistry & Molecular Biology
Eisner Salamanca, Yu-Hwa Pan, Ying-Sui Sun, Hao-Wen Hsueh, Odontuya Dorj, Wan-Ling Yao, Jerry Chin-Yi Lin, Nai-Chia Teng, Ikki Watanabe, Shinichi Abe, Yi-Fan Wu, Wei-Jen Chang
Summary: This study aimed to improve the properties of β-TCP by adding magnesium through hydrothermal methods, resulting in enhanced alkaline phosphatase activity and cell viability, promoting bone regeneration.β-TCP/Mg demonstrated potential application prospects in bone grafting and tissue engineering treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Hyeong Seok Kim, Wookyeong Song, Wonho Choo, Sungyoung Lee, Youngmin Han, Claudio Bassi, Roberto Salvia, Giovanni Marchegiani, Christopher L. Wolfgang, Jin He, Alex B. Blair, Michael D. Kluger, Gloria H. Su, Song Cheol Kim, Ki-Byung Song, Masakazu Yamamoto, Takashi Hatori, Ching-Yao Yang, Hiroki Yamaue, Seiko Hirono, Sohei Satoi, Tsutomu Fujii, Satoshi Hirano, Wenhui Lou, Yasushi Hashimoto, Yasuhiro Shimizu, Marco Del Chiaro, Roberto Valente, Matthias Lohr, Dong Wook Choi, Seong Ho Choi, Jin Seok Heo, Fuyuhiko Motoi, Ippei Matsumoto, Woo Jung Lee, Chang Moo Kang, Yi-Ming Shyr, Shin-E Wang, Ho-Seong Han, Yoo-Seok Yoon, Marc G. Besselink, Nadine C. M. van Huijgevoort, Masayuki Sho, Hiroaki Nagano, Sang Geol Kim, Goro Honda, Yinmo Yang, Hee Chul Yu, Jae Do Yang, Jun Chul Chung, Yuichi Nagakawa, Hyung Il Seo, Seungyeoun Lee, Hongbeom Kim, Wooil Kwon, Taesung Park, Jin-Young Jang
Summary: A novel nomogram based on radiologic findings of intraductal papillary mucinous neoplasms (IPMN) was developed and compared with existing American and Korean/Japanese nomograms. The results showed that this nomogram performed better than the American nomogram and was comparable to the Korean/Japanese nomogram. This nomogram would be clinically helpful in situations where tumor markers are not available.
JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
So Dam Lee, Haengdueng Jeong, Bo Ram Hwang, Byeong Min Yu, Yejin Cho, Ki Teak Nam, Hyunki Kim, Yong Chan Lee
Summary: Strains of Helicobacter pylori positive for CagA may contribute to the development of gastric cancer through the induction of CK2 activation and CK28 degradation. The downregulation of CK28 and subsequent activation of AKT and Snail signaling pathways may play a crucial role in the epithelial-to-mesenchymal transition (EMT) in gastric cancer. CK2 could be a potential molecular target for the treatment of H. pylori-infected gastric cancer.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Gastroenterology & Hepatology
Yeon Seung Chung, Youngsic Jeon, Jeong Eun Yoo, Taek Chung, Hyang Joo Ryu, Hyunki Kim, Hyungjin Rhee, Young Nyun Park
Summary: In this study, a method was developed to differentiate intrahepatic cholangiocarcinomas (iCCAs) from adenocarcinomas originating from extrahepatic organs adjacent to the liver. The combination of albumin mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) staining for filamin-A and cytokeratin 19 (CK19) showed better diagnostic performance than albumin ISH alone. The newly developed iCCA-score consisting of these three markers can accurately differentiate iCCA from extrahepatic adenocarcinomas.
HEPATOLOGY INTERNATIONAL
(2023)
Article
Oncology
Jung Yong Hong, Hee Jin Cho, Kum-Hee Yun, Young Han Lee, Seung Hyun Kim, Wooyeol Baek, Sang Kyum Kim, Yurimi Lee, Yoon-La Choi, Minsuk Kwon, Hyo Song Kim, Jeeyun Lee
Summary: This study investigated the molecular determinants of pazopanib response in refractory soft tissue sarcoma patients. It identified CDK4 amplification as a predictor of poor response and shorter progression-free survival. Additionally, the combination of pazopanib and durvalumab showed efficacy in patients with high immune/stromal cell infiltration and increased CD19 expression.
CANCER RESEARCH AND TREATMENT
(2023)
Article
Biophysics
Rui Tang, Kaelyn L. Gasvoda, Jacob Rabin, Eben Alsberg
Summary: Thermally stable proteins are important for resisting irreversible denaturation during thermal stress. In this study, the conformational and thermal stability of TGF-beta 1 were investigated, revealing its ability to recover native folding after thermal treatment. Further studies demonstrated the maintenance of its bioactive properties and potential applications in biomedicine and nutrition.
BIOPHYSICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Kwang Sik Lee, Bo Yeon Kim, Yun Hui Kim, Yong Soo Choi, Byung Rae Jin
Summary: In this study, a novel protein component called Amwaprin was identified and characterized from honeybee venom. Amwaprin showed inhibitory activity against microbial serine proteases and elastases, as well as microbicidal activity by damaging bacterial and fungal cell walls. It acts as a microbicidal and anti-elastolytic agent.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Nariman Nezami, Alex Behi, Sivasai Manyapu, Jane L. Meisel, Neil Resnick, David Corn, J. David Prologo
Summary: This study aimed to evaluate the feasibility, safety, and efficacy of intercostobrachial nerve (ICBN) cryoneurolysis for pain control in patients with postmastectomy pain syndrome (PMPS). Fourteen patients with PMPS underwent CT-guided percutaneous cryoneurolysis of the ICBN, resulting in a significant decrease in pain scores and interference with daily activities for up to 6 months. The procedure was technically successful in all patients, and no adverse events were reported.
JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY
(2023)
Article
Oncology
Young Soo Park, Myeong-Cherl Kook, Baek-hui Kim, Hye Seung Lee, Dong-Wook Kang, Mi-Jin Gu, Ok Ran Shin, Younghee Choi, Wonae Lee, Hyunki Kim, In Hye Song, Kyoung-Mee Kim, Hee Sung Kim, Guhyun Kang, Do Youn Park, So -Young Jin, Joon Mee Kim, Yoon Jung Choi, Hee Kyung Chang, Soomin Ahn, Mee Soo Chang, Song-Hee Han, Yoonjin Kwak, An Na Seo, Sung Hak Lee, Mee-Yon Cho
Summary: This edition is composed of standard data elements and conditional data elements. Standard data elements contain basic pathological findings and necessary items to predict the prognosis of GC patients, while conditional data elements include additional diagnostic and prognostic factors relevant to adjuvant therapy, such as molecular biomarkers. They are designed to allow pathologists to selectively choose appropriate items based on their institution's environment. The standardized pathology report aims to improve GC diagnosis and facilitate large-scale research.
JOURNAL OF GASTRIC CANCER
(2023)
Review
Pathology
Young Soo Park, Myeong-Cherl Kook, Baek-hui Kim, Hye Seung Lee, Dong-Wook Kang, Mi-Jin Gu, Ok Ran Shin, Younghee Choi, Wonae Lee, Hyunki Kim, In Hye Song, Kyoung-Mee Kim, Hee Sung Kim, Guhyun Kang, Do Youn Park, So -Young Jin, Joon Mee Kim, Yoon Jung Choi, Hee Kyung Chang, Soomin Ahn, Mee Soo Chang, Song-Hee Han, Yoonjin Kwak, An Na Seo, Sung Hak Lee, Mee-Yon Cho
Summary: The first edition of 'A Standardized Pathology Report for Gastric Cancer' was initiated by the Gastrointestinal Pathology Study Group of the Korean Society of Pathologists and published 17 years ago. This second edition consists of standard data elements and conditional data elements, including basic pathologic findings and items necessary for predicting the prognosis of GC patients. Other factors relevant to adjuvant therapy are classified as conditional data elements. The standardized pathology report will be helpful for GC diagnosis and facilitate collaborative studies.
JOURNAL OF PATHOLOGY AND TRANSLATIONAL MEDICINE
(2023)
Article
Entomology
Yun Hui Kim, Bo Yeon Kim, Yong Soo Choi, Kwang Sik Lee, Byung Rae Jin
Summary: The study found that feeding nurse bees peppermint oil (PO) and a cocktail of pathogens can promote the induction of immune memory, increase body weight, and upregulate marker genes.
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
(2023)
Review
Engineering, Biomedical
Bikram Adhikari, Michael A. Stager, Melissa D. Krebs
Summary: The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. Recent developments focus on biomaterials that direct cell behavior using properties such as charge, peptide presentation, and micro- or nano-geometry, which could significantly advance the development of clinically relevant medical devices and enhance patient care.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Chemistry, Multidisciplinary
Sung-Won Hwang, Chung-Man Lim, Cong Truc Huynh, Hossein Moghimianavval, Nicholas A. Kotov, Eben Alsberg, Allen P. Liu
Summary: This study presents a strategy to confer mechano-responsive behavior on hydrogels by embedding hybrid vesicles. Under mechanical stress, these vesicles release encapsulated compounds that control the hydrogel network, offering promise for diverse biomedical applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Fisheries
Yun Hui Kim, Bo Yeon Kim, Yong Soo Choi, Kwang Sik Lee, Byung Rae Jin
Summary: Feeding honeybees with a heat-killed pathogen cocktail can induce transgenerational immune responses, as shown by the upregulation of vitellogenin and defensin-1 in nurse bees. The Toll signaling pathway and the expression of defensin-1 were also upregulated upon ingestion of the pathogen cocktail. Furthermore, the introduction of the cocktail resulted in increased resistance to pathogen infection in worker larvae.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kari Martyniak, Sean Kennedy, Makan Karimzadeh, Maria A. Cruz, Oju Jeon, Eben Alsberg, Thomas J. Kean
Summary: In this study, a high-throughput human articular chondrocyte reporter system was used to optimize the combination of GelMA and OMA for lubricin expression. The 14% GelMA / 2% OMA formulation was identified as the optimal one for lubricin secretion. This strategy allows for rapid analysis of the effects of biomaterial composition and cell manipulation on lubricin expression, which could enhance therapeutic strategies for cartilage regeneration.
BIOENGINEERING-BASEL
(2023)
Article
Polymer Science
Michael A. Stager, Bikram Adhikari, Alexandra Raichart, Melissa D. Krebs
Summary: Hydrogels are important wound-healing materials that are usually synthesized using a radical polymerization approach. However, the development of initiator-free hydrogels has gained attention due to their excellent cytocompatibility and ease of scale-up. In this study, initiator-free zwitterionic hydrogels were developed using a photopolymerization process under cytocompatible conditions. These hydrogels exhibited a wide range of mechanical characteristics, resist nonspecific protein adsorption, and showed sustained release of proteins and small molecules. Additionally, these self-initiated hydrogels significantly reduced inflammatory macrophage activation in vitro.